Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 880,369 T→C 6.6% I38V (ATA→GTA)  gstB ← glutathione S‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913880,3690TC 6.6% 279.6 / 7.2 92I38V (ATA→GTA) gstBglutathione S‑transferase
Reads supporting (aligned to +/- strand):  ref base T (48/37);  new base C (3/3);  total (52/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

CGATCCACAGGCGTTTTTGCCCGTACTGTGCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGTAATCATACTCACCCCTTTTCCGACAGGCAGCCACAGGCT  >  NC_000913/880222‑880513
                                                                                                                                                   |                                                                                                                                                
cGATCCACAGGCGTTTTTGCCCGTACTGTGCCGCCAGATAGCGGACAGTGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTAt                                                                                                                                                  >  7:231305/1‑148 (MQ=255)
  aTCCACAGGCGTTTTTGCCCGTACTGTGCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATcc                                                                                                                                                >  2:127183/1‑148 (MQ=255)
    ccACAGGCGTTTTTGCCCGTACTGTGCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATccc                                                                                                                                               >  3:55609/1‑147 (MQ=255)
      acaGGCGTTTTTGCCCGTACTGTGCCGCCAGATAGCGGACAATGGCGTTTGATGCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCAAGAAAATCAGCATCGTGATTTATCCCaaa                                                                                                                                            <  5:70523/148‑1 (MQ=255)
          gCGTTTTTGCCCGTACTGTGCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCa                                                                                                                                        <  5:34300/148‑1 (MQ=255)
           cGTTTTTGCCCGTACTGTGCCGCCAGATAGCGGACAATGGCGTTTGCTTCCCAAAGAATGAGATCACTTTCGTCGTCTCGCAACACCGGCACCAGCCCGGGAGGGTTCATCGCCAGAACATCAGCATCGGGATTTACCCCAAACTccc                                                                                                                                       >  7:249729/1‑146 (MQ=255)
           cGTTTTTGCCCGTACTGTGCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCTCGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTACCCCAAACTCAc                                                                                                                                       >  7:249727/1‑148 (MQ=255)
           cGTTTTTGCCCGTACTGTGCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCTCGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTACCCCAAACTCAc                                                                                                                                       >  7:255562/1‑148 (MQ=255)
             tttttGCCCGTACTGTGCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACgg                                                                                                                                     >  4:274014/1‑148 (MQ=255)
              ttttGCCCGTACTGTGCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGc                                                                                                                                    >  7:140428/1‑148 (MQ=255)
                   cccGTACTGTGCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCcgcg                                                                                                                               <  2:241768/148‑1 (MQ=255)
                    ccGTACTGTGCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCCGCCCGTTAGGGTACATCGCCAGAAAATCAGCATCGAGAATTAACCCAAACTCACGTCCcccg                                                                                                                               >  2:38576/1‑147 (MQ=255)
                     cGTACTGTGCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCCCGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCcgc                                                                                                                                >  1:331069/1‑145 (MQ=255)
                      gTACTGTGCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCgag                                                                                                                             >  8:316491/1‑147 (MQ=255)
                      gTACTGTGCCGCCAGATAGCGGACAATGGCGTTTGATTCACAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGAACCAGCCCGGTAGGGTTCATCGCCAGTAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCgag                                                                                                                             >  5:193475/1‑147 (MQ=255)
                        aCTGTGCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCCCCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAAt                                                                                                                          >  2:206733/1‑148 (MQ=255)
                         cTGTGCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAAt                                                                                                                          <  6:339568/147‑1 (MQ=255)
                          tgtgCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAAttt                                                                                                                        >  6:262240/1‑148 (MQ=255)
                           gtgCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGTTTTATCCCAAACTCACGGCCCGCGAGAATTTg                                                                                                                       >  2:255392/1‑148 (MQ=255)
                           gtgCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTg                                                                                                                       >  4:41768/1‑148 (MQ=255)
                            tgCCGCCAGATAGCTGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCACGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTATCCCAAACTCACGGCCCGCGAGAATTTg                                                                                                                       <  6:8162/146‑1 (MQ=255)
                                  cAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCGAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATaa                                                                                                                <  4:112320/148‑1 (MQ=255)
                                    gATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAgg                                                                                                              >  1:130526/1‑148 (MQ=255)
                                    gATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTACATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAgg                                                                                                              >  6:375501/1‑148 (MQ=255)
                                     aTAGCGTACAATGGCGTTTGATTCGCCAAGAATGAGATCACTTTCGTCGTCACTCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCCGCATCGTGATTTATCCCAAACTCACGGCCCGCTAGAATTTTCTCATAAGGt                                                                                                             <  6:81603/148‑1 (MQ=255)
                                     aTAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGCTTTATCCCAACCTCACGGCCCGCGAGAATTTGCTCATAAGGt                                                                                                             <  3:186046/148‑1 (MQ=255)
                                      tAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTa                                                                                                            <  7:72229/148‑1 (MQ=255)
                                        gCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAg                                                                                                           <  4:55609/147‑1 (MQ=255)
                                         cGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGtt                                                                                                         <  1:127183/148‑1 (MQ=255)
                                          ggACAATGGCGGTTGATTCCCAAAGAATGCGAGCACTTTCGGCGTCCCGCCACAACGGCACCAGCCCGTTAGGGTTCCTCGCCAGAAACTCAGCATCGGGCTTTATCCCAACCTCACGGCCCGCGAGAAttt                                                                                                                        <  3:177423/132‑1 (MQ=255)
                                                 ggCGTTTGATTACCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCTCGCGAATTTGCTCATAAGGTAGTTCCAGttct                                                                                                 >  5:241375/1‑148 (MQ=255)
                                                  gCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGTCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGttctt                                                                                                <  8:45134/148‑1 (MQ=255)
                                                      ttGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAg                                                                                            >  7:76024/1‑148 (MQ=255)
                                                      gtGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCCTCGCCAGAAGATCCGCATCGTGATTTATCCCAACCTCCCGGCCCGCGAGACTTTGCTCATAAGGTAGTTCCAGTTCGTCGAg                                                                                            >  6:357678/2‑148 (MQ=255)
                                                         aTTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGt                                                                                         <  5:262240/148‑1 (MQ=255)
                                                             ccAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAg                                                                                      >  8:278438/1‑147 (MQ=255)
                                                              cAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGc                                                                                     >  1:214571/1‑147 (MQ=255)
                                                               aaaGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCTGCCCGTTAGGGTTCATCGCCAGAAAATCCGCATCGTGATTTATACCAAACTCACGGCCCGCGAGAATTTTATCATAAGGTAGTTCCAGTTATTCGAGCGTCAGCaa                                                                                   >  5:330072/1‑148 (MQ=255)
                                                               aaaGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCaa                                                                                   >  2:220210/1‑148 (MQ=255)
                                                                aaGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAAATTCCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCat                                                                                   >  7:23774/1‑146 (MQ=255)
                                                                     tGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACttt                                                                             <  8:177016/148‑1 (MQ=255)
                                                                        gaTCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACtttttt                                                                          <  7:779/148‑1 (MQ=255)
                                                                         aTCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACttttttt                                                                         <  7:316491/148‑1 (MQ=255)
                                                                         aTCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACtttttt                                                                          >  6:146904/1‑147 (MQ=255)
                                                                              tttCGTAGTCACGCAACAACGGCACCAGCCCGGTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTa                                                                                                            <  4:203531/108‑1 (MQ=255)
                                                                               ttCGTCGTCACGCAACACCGGCACCAGCCCGTTAGGGTTCATCGCCTGAAAATCAGCATCGTGATTTATGCCGACCTCAGGGCCCGCGAGAATTTGCTCATAAGGTac                                                                                                           >  3:203531/1‑107 (MQ=255)
                                                                                tcgtcgTCTCGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGtt                                                                    <  6:193475/146‑1 (MQ=255)
                                                                                tcgtcgTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGtt                                                                    <  1:366284/146‑1 (MQ=255)
                                                                                  gtcgtcACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGt                                                                 <  3:274014/147‑1 (MQ=255)
                                                                                      tcACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACt                                                                               >  1:54939/1‑129 (MQ=255)
                                                                                      tcACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACt                                                                               <  2:54939/129‑1 (MQ=255)
                                                                                      tcACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAat                                                            <  4:172037/148‑1 (MQ=255)
                                                                                       cACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGt                                                                                                             >  7:75751/1‑98 (MQ=255)
                                                                                       cACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGt                                                                                                             <  8:75751/98‑1 (MQ=255)
                                                                                       cACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAat                                                            >  7:304335/1‑147 (MQ=255)
                                                                                        aCGCAACACCGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCACGATCGTGATTTATCGCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAatt                                                           <  1:131455/147‑1 (MQ=255)
                                                                                        aCGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAatta                                                          <  5:22393/148‑1 (MQ=255)
                                                                                         cGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGCTTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAattat                                                         <  1:38576/148‑1 (MQ=255)
                                                                                          gCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAattatt                                                        <  8:140428/148‑1 (MQ=255)
                                                                                           caacaaCGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAattat                                                         <  1:207571/146‑1 (MQ=255)
                                                                                           caacaaCGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAattat                                                         >  2:207571/1‑146 (MQ=255)
                                                                                           caacaaCGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTc                                                       >  1:57792/1‑148 (MQ=255)
                                                                                           caacaaCGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGGTCTTCGAGCGTCCGCAATACTTTTTTTACGTTAGTTGAATTATTc                                                       >  8:358266/1‑148 (MQ=255)
                                                                                             acaacGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTTGTTGAATTATTCCg                                                     >  2:46997/1‑148 (MQ=255)
                                                                                             acaacGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCg                                                     <  1:220210/148‑1 (MQ=255)
                                                                                              caacGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGTTTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGa                                                    <  1:255392/148‑1 (MQ=255)
                                                                                              caacGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGa                                                    <  5:146904/148‑1 (MQ=255)
                                                                                                    cACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCcac                                              <  3:41768/148‑1 (MQ=255)
                                                                                                     aCCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCca                                               >  4:249282/1‑146 (MQ=255)
                                                                                                      ccAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTAAGTTAGTTGAATTATTCCGACCCCACAg                                            >  2:142209/1‑148 (MQ=255)
                                                                                                       cAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTACCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGc                                           <  8:249727/148‑1 (MQ=255)
                                                                                                       cAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTACCCCAAACTCACGGCCCGCGAGAATTTGCTCATAACGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGc                                           <  8:255562/148‑1 (MQ=255)
                                                                                                       cAGCCCGTTAGGGGTCATCGCCAGAAAATCAGCATCGTGATTTACCCCAAACTCACGGCCCGCGAGAATGTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGGCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGc                                           <  8:249729/148‑1 (MQ=255)
                                                                                                         gCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCg                                          >  4:105811/1‑147 (MQ=255)
                                                                                                           ccGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGTaa                                       >  7:376979/1‑148 (MQ=255)
                                                                                                            cGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGTAAt                                      <  3:178301/148‑1 (MQ=255)
                                                                                                                             aGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGCGCGTCAGCAATACTTTTTTTACGTTTGTTGAATTATTCCGACCCCCCAGCGTAATCGTACTCCCCCCTTTTc                     >  8:368884/1‑148 (MQ=255)
                                                                                                                             aGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGTAATCATACTCACCCCTTTTc                     >  3:246078/1‑148 (MQ=255)
                                                                                                                                 aaTCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGTAATCATACTCACCCCTTTTCCGAc                 <  5:375501/148‑1 (MQ=255)
                                                                                                                                          cGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGTAATCATACTCACCCCTTTTCCGACAGGCAGCca        >  8:116860/1‑148 (MQ=255)
                                                                                                                                          cGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGTAATCATACTCACCCCTTTTCCGACAGGCAGCca        >  7:264100/1‑148 (MQ=255)
                                                                                                                                          cGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGTAATCATACTCACCCCTTTTCCGACAGGCAGCca        >  3:167233/1‑148 (MQ=255)
                                                                                                                                          cGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGTAATCATACTCACCCCTTTTCCGACAGGCAGCca        >  1:203559/1‑148 (MQ=255)
                                                                                                                                          cGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGTAATCATACTCACCCCTTTTACGACAGGCAGCca        >  1:331206/1‑148 (MQ=255)
                                                                                                                                          cGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGTAATCATACTCACACCTTTTCCGACAGGCAGCca        >  5:108775/1‑148 (MQ=255)
                                                                                                                                            tGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGTAATCATACTCACCCCTTTTCCGACAGGCAGCca        >  1:120629/1‑146 (MQ=255)
                                                                                                                                             gATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGTAATCATACTCACCCCTTTTCCGACAGGCAGCCACAg     >  3:127000/1‑148 (MQ=255)
                                                                                                                                              aTTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGt                                                                 >  7:75109/1‑87 (MQ=255)
                                                                                                                                              aTTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGt                                                                 <  8:75109/87‑1 (MQ=255)
                                                                                                                                              aTTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGTAATCATACTCACCCCTTTTCCGACAGGCAGCCACAgg    >  3:293994/1‑148 (MQ=255)
                                                                                                                                                ttATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGTAATCATACTc                               >  3:254262/1‑119 (MQ=255)
                                                                                                                                                ttATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGTAATCATACTc                               <  4:254262/119‑1 (MQ=255)
                                                                                                                                                ttATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGAAATCATACTCACCCCTTTTCCGACAGGCAGCCACAGGCt  >  5:173236/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CGATCCACAGGCGTTTTTGCCCGTACTGTGCCGCCAGATAGCGGACAATGGCGTTTGATTCCCAAAGAATGAGATCACTTTCGTCGTCACGCAACAACGGCACCAGCCCGTTAGGGTTCATCGCCAGAAAATCAGCATCGTGATTTATCCCAAACTCACGGCCCGCGAGAATTTGCTCATAAGGTAGTTCCAGTTCTTCGAGCGTCAGCAATACTTTTTTTACGTTAGTTGAATTATTCCGACCCCACAGCGTAATCATACTCACCCCTTTTCCGACAGGCAGCCACAGGCT  >  NC_000913/880222‑880513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: