Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 77,914 A→G 5.3% A98A (GCA→GCG setA → broad specificity sugar efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091377,9140AG 5.3% 233.2 / 3.1 75A98A (GCA→GCGsetAbroad specificity sugar efflux system
Reads supporting (aligned to +/- strand):  ref base A (38/33);  new base G (2/2);  total (40/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

ATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGT  >  NC_000913/77771‑78061
                                                                                                                                               |                                                                                                                                                   
aTCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGTCAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCAttt                                                                                                                                                  >  1:117948/1‑147 (MQ=255)
aTCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCAttt                                                                                                                                                  >  1:182517/1‑147 (MQ=255)
 tCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTaa                                                                                                                                                >  7:41847/1‑148 (MQ=255)
  cGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAAt                                                                                                                                               >  7:276425/1‑148 (MQ=255)
    gCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAAtag                                                                                                                                             >  1:326987/1‑146 (MQ=255)
      ctctTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGTATTTAATCGTc                                                                                                                                           <  8:356410/148‑1 (MQ=255)
      ctctTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGt                                                                                                                                            >  5:165654/1‑147 (MQ=255)
      ctctTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGt                                                                                                                                            >  4:105029/1‑147 (MQ=255)
        ctTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAACACGTTCTGACAGTCAGGGCGATCGGCTAAACCTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGATAGTGTTTGAATTTAATAGTCAt                                                                                                                                         >  6:354528/1‑148 (MQ=255)
        ctTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCAt                                                                                                                                         >  4:158549/1‑148 (MQ=255)
         ttttATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCAt                                                                                                                                         >  8:112951/1‑147 (MQ=255)
          tttATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGTTTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTa                                                                                                                                       >  6:116457/1‑148 (MQ=255)
                cGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGAc                                                                                                                                 >  2:3184/1‑148 (MQ=255)
                             tGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTc                                                                                                                                           >  8:221962/1‑125 (MQ=255)
                             tGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTc                                                                                                                                           <  7:221962/125‑1 (MQ=255)
                              gCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATc                                                                                                                                     >  2:146636/1‑130 (MQ=255)
                              gCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATc                                                                                                                                     <  1:146636/130‑1 (MQ=255)
                              gCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCtgt                                                                                                                    <  3:105029/147‑1 (MQ=255)
                               cTGGGAGCGGCGTAAGCCTCTGGTTGGCAAAACGGTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTgtgg                                                                                                                  <  4:353483/148‑1 (MQ=255)
                                    aTCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCATGGCGATCGGCGAAACCAGATTATATTTTGCTGTTTGATTGCTATCGGCTCTGCGCGAGTGTGTGCATTTAATCGTCATTATCTGACGCTTATCCCCTGTGGTGTGc                                                                                                             >  6:186699/1‑148 (MQ=255)
                                    aTCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGTGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCGTTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGc                                                                                                             >  5:154793/1‑148 (MQ=255)
                                    aTCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCGTTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGc                                                                                                             >  3:53284/1‑148 (MQ=255)
                                        gCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCt                                                                                                         <  3:158549/148‑1 (MQ=255)
                                         cGTAAGCCTCTGGTTGGCAATACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACg                                                                                                                                <  4:204221/124‑1 (MQ=255)
                                         cGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACg                                                                                                                                >  3:204221/1‑124 (MQ=255)
                                         cGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTgtgg                                                                                                                  <  7:158520/138‑1 (MQ=255)
                                         cGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTgtgg                                                                                                                  >  8:158520/1‑138 (MQ=255)
                                            aaGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTgtgg                                                                                                                  >  5:265034/1‑135 (MQ=255)
                                            aaGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTgtgg                                                                                                                  <  6:265034/135‑1 (MQ=255)
                                              gCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCAtc                                                                                                   <  4:284139/148‑1 (MQ=255)
                                                         gCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAAt                                                                                         <  2:211093/147‑1 (MQ=255)
                                                         gCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAAt                                                                                         <  5:373515/147‑1 (MQ=255)
                                                         gCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATa                                                                                        <  5:116457/148‑1 (MQ=255)
                                                            aaaCGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACgg                                                                                     >  1:72445/1‑148 (MQ=255)
                                                             aaCGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCTTAATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATActg                                                                                     >  5:363569/1‑145 (MQ=255)
                                                                          tCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTa                                                                       >  1:36808/1‑148 (MQ=255)
                                                                          tCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTa                                                                       <  7:47165/148‑1 (MQ=255)
                                                                             gggCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGAAGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACTGCAATGACACTGTTAttt                                                                    >  6:157199/1‑148 (MQ=255)
                                                                               gCGATCGGCGAAAACTGATTATATTTTTCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATg                                                                                >  3:81056/1‑134 (MQ=255)
                                                                               gCGATCGGCGAAAACTGATTATATTTTCCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATg                                                                                <  4:81056/134‑1 (MQ=255)
                                                                                cGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGct                                                                 <  8:41847/148‑1 (MQ=255)
                                                                                cGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGct                                                                 <  6:165654/148‑1 (MQ=255)
                                                                                 gagcgccGAAAACAGATTATATTTTGCTCTTTTATCGCTAGCGGAAATGCTCTATTGTTTGCATTTAATCTTCATTATCTGACGCTTATCACCTTTTGTGTGCTTCTGGCATCTCTGGCCAATACTGCAATGCCACAGTTATTTGctc                                                                <  2:90447/142‑1 (MQ=255)
                                                                                  aTCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGctct                                                               >  1:302699/1‑148 (MQ=255)
                                                                                        gAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGcgcg                                                         <  1:192295/148‑1 (MQ=255)
                                                                                          aaaCTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCgg                                                        >  6:91055/1‑147 (MQ=255)
                                                                                             cTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGGGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAat                                                    <  2:326987/148‑1 (MQ=255)
                                                                                              tGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCCTCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAata                                                   >  4:165794/1‑148 (MQ=255)
                                                                                                aTTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCGTTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAata                                                   <  6:154793/146‑1 (MQ=255)
                                                                                                aTTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCGTTTAATCGTCATTATCTGACGCTTATCACCTGTGGGGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAata                                                   <  4:53284/146‑1 (MQ=255)
                                                                                                aTTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCAtct                                                                                                  <  1:215996/99‑1 (MQ=255)
                                                                                                aTTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATg                                                 <  5:79525/148‑1 (MQ=255)
                                                                                                aTTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATAACCTGTGGTGTGCTTCTGGCAtct                                                                                                  >  2:215996/1‑99 (MQ=255)
                                                                                                    tatTTTGCTGTTTGAGGGCTATCGGCAATGCGCTATTGTTCGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATACGCGGa                                             <  8:302940/148‑1 (MQ=255)
                                                                                                             gTTTGATGGCTATCGGCATTGCGCTATTGTTTGCATTTAATGGTCTTTATCTGAGGCTTATCTCCTGTGGTGTGCTTCTGGCATCTCTGGCCACTACGGCAATTCCAGAGTTATTTGCTCTGGCGCGGGTATATGCGGATAACTCGgc                                    >  3:194215/1‑148 (MQ=255)
                                                                                                             gTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGgc                                    >  8:71596/1‑148 (MQ=255)
                                                                                                             gTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGgc                                    >  2:222391/1‑148 (MQ=255)
                                                                                                             gTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACACGgc                                    >  7:193877/1‑148 (MQ=255)
                                                                                                              tttGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGgcg                                   <  1:3184/148‑1 (MQ=255)
                                                                                                                      cTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAgt                           <  8:115282/148‑1 (MQ=255)
                                                                                                                         tCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAgtg                          <  1:222391/146‑1 (MQ=255)
                                                                                                                           ggCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAgtggtg                       <  2:117948/147‑1 (MQ=255)
                                                                                                                           ggCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGa                      <  2:36808/148‑1 (MQ=255)
                                                                                                                                tGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATATCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGtt                  >  3:101244/1‑147 (MQ=255)
                                                                                                                                 gcgcTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGtt                  >  2:162237/1‑146 (MQ=255)
                                                                                                                                  cgcTATTGTTTGCATTTAATCGTAATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGCCAATGCCACAGTTATTTGCTCGGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGAAGTTTa                <  5:186699/147‑1 (MQ=255)
                                                                                                                                    cTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGGGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCt             >  1:154698/1‑148 (MQ=255)
                                                                                                                                      aTTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCg           >  8:11926/1‑148 (MQ=255)
                                                                                                                                      aTTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCg           >  5:223806/1‑148 (MQ=255)
                                                                                                                                       ttGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCgg          >  5:137132/1‑148 (MQ=255)
                                                                                                                                          tttGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTg        <  7:71596/147‑1 (MQ=255)
                                                                                                                                          tttGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTg        <  1:162237/147‑1 (MQ=255)
                                                                                                                                          tttGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGa       <  5:91055/148‑1 (MQ=255)
                                                                                                                                           ttGCATTTAATCGTGATTTTCTTACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACTGCAATGCGACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAAGTCGGCGCGAGAAGTGGTGATGTTTAGCGCGGGGa       <  8:193877/147‑1 (MQ=255)
                                                                                                                                           ttGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGCTGTTTAGCTCGGTGAt      >  2:350749/1‑148 (MQ=255)
                                                                                                                                               aTTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtgcgt  <  4:74399/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
ATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGAAAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGT  >  NC_000913/77771‑78061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: