Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,052,264	A→G	5.8%	intergenic (+24/+25)	gnsA → / ← yccM	putative phosphatidylethanolamine synthesis regulator/putative 4Fe‑4S membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,052,264	0	A	G	5.8%	265.3 / 5.6	86	intergenic (+24/+25)	gnsA/yccM	putative phosphatidylethanolamine synthesis regulator/putative 4Fe‑4S membrane protein
Reads supporting (aligned to +/- strand):  ref base A (34/47);  new base G (2/3);  total (36/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

GCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCA  >  NC_000913/1052121‑1052410                                                                                                                                                |                                                                                                                                                   gcgcAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGt                                                                                                                                                 <  6:6755/147‑1 (MQ=255)       aaaaTCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTc                                                                                                                                          >  6:218292/1‑148 (MQ=255)             gCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCtt                                                                                                                                     <  4:258239/147‑1 (MQ=255)              ccGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTTAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCttgt                                                                                                                                   <  3:175970/148‑1 (MQ=255)                     gAACAAGAATACTGGGAAAGAAGTCTCTGAAATTCTCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAAt                                                                                                                            <  6:86859/148‑1 (MQ=255)                      aaCAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTaa                                                                                                                             <  1:35927/146‑1 (MQ=255)                      aaCAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTGGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGGGATGTTCCGCTTGTTGCTAAt                                                                                                                            <  3:325390/147‑1 (MQ=255)                       aCAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAAttt                                                                                                                          <  1:197889/148‑1 (MQ=255)                       aCAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTC‑AAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTa                                                                                                                         <  2:277265/148‑1 (MQ=255)                        cAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTa                                                                                                                         <  8:361483/148‑1 (MQ=255)                         aaGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAg                                                                                                                        >  5:288875/1‑148 (MQ=255)                         aaGAATACAGGGAAAGAAGTCTCTGAAATTAGCTTCACCTCACGAGAAAAAATGACCGTGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAg                                                                                                                        <  3:194075/148‑1 (MQ=255)                           gAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATATAGTg                                                                                                                      <  3:158597/148‑1 (MQ=255)                            aaTACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGGTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGa                                                                                                                     <  3:327273/148‑1 (MQ=255)                              tACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAATGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTATTGAc                                                                                                                    >  8:90473/1‑147 (MQ=255)                               aCAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGAcc                                                                                                                   >  5:44856/1‑147 (MQ=255)                                  gggAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATc                                                                                                               <  4:299722/148‑1 (MQ=255)                                  gggAAAGAAGTCTCTGAAATTCGCTTCACCGCAAGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTTATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATc                                                                                                               <  3:355908/148‑1 (MQ=255)                                     aaaTAAGTGTCAGAAATTCGCTTCACCGCACGAGAAAAAATGAGCGTGCTTGAAAGTTATGAAGTCAAATTCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCAtt                                                                                                            <  3:193890/148‑1 (MQ=255)                                      aagaagTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGGGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTg                                                                                                           <  1:361166/148‑1 (MQ=255)                                      aagaagTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTAAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTg                                                                                                           >  5:72545/1‑148 (MQ=255)                                         aagTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGgcg                                                                                                        >  3:287424/1‑148 (MQ=255)                                          agTCTCTGAAATTCGCTTCTCCGCACGAGAAAAAATGACCGGGCTTGATTGTTATGATGTCTATATGAATATAATGTGATTTTGTGATCATCGCCCCGTGCGAGGTGATGTTCCGCTTGTTGGTTTTTTAGTGACGTCTCATTGgcgc                                                                                                       >  2:202595/1‑148 (MQ=255)                                            tctcTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCtt                                                                                                     >  7:80572/1‑148 (MQ=255)                                             ctctGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTtg                                                                                                    >  6:268134/1‑148 (MQ=255)                                               ctGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTtgtg                                                                                                  >  2:211803/1‑148 (MQ=255)                                               ctGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTtgtg                                                                                                  >  7:122879/1‑148 (MQ=255)                                                tGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCttgttg                                                                                                                                 >  5:303667/1‑116 (MQ=255)                                                tGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCttgttg                                                                                                                                 <  6:303667/116‑1 (MQ=255)                                                   aaTTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGaa                                                                                               <  6:288875/147‑1 (MQ=255)                                                    aTTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAAtt                                                                                             <  4:142321/148‑1 (MQ=255)                                                     ttCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTTTGAACATCACCACGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTTACCAATCATTGGCGCTTGTTGAATTa                                                                                            <  6:82726/148‑1 (MQ=255)                                                         cTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCg                                                                                        <  2:376314/148‑1 (MQ=255)                                                            cACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTc                                                                                      <  4:13255/147‑1 (MQ=255)                                                             aCCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAAttt                                                                                                                          <  3:349462/110‑1 (MQ=255)                                                             aCCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAAttt                                                                                                                          >  4:349462/1‑110 (MQ=255)                                                             aCCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCgg                                                                                    <  8:122879/148‑1 (MQ=255)                                                               cGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTa                                                                                  <  3:119314/148‑1 (MQ=255)                                                               cGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTa                                                                                  <  1:29517/148‑1 (MQ=255)                                                                 cACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCTTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACa                                                                                <  2:252553/148‑1 (MQ=255)                                                                 cACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTTATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACa                                                                                <  3:235295/148‑1 (MQ=255)                                                                 cACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCTCTTGTGGAATTAAGCGTCGGTACa                                                                                <  1:211803/148‑1 (MQ=255)                                                                 cACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACa                                                                                <  1:137835/148‑1 (MQ=255)                                                                 cACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACa                                                                                <  3:40442/148‑1 (MQ=255)                                                                     agaAAAAATGACCGGGCTTGAAAGTTATTATGTCAAAATCAAAATAATCTTATTTTGTGAACATCACCCCTAGCGAGCTGATGTTCCGATTGTTGATAATTTAGTGACCAATCAATGGCGATTGTGGAATTAAGCGTCGGTTAAAtt                                                                             >  5:279560/1‑147 (MQ=255)                                                                     agaAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAAt                                                                              >  4:100678/1‑146 (MQ=255)                                                                     agaAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAAt                                                                              <  3:100678/146‑1 (MQ=255)                                                                       aaaaaaTGACCGGGCTTGAAAGTTATGATGTCAAATTCAAAATAATGTGATTTTGTGAACATCTCCCAGTGGGAGTTGATGTTCGGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTTTTGAATTAAGAGTCGGTACAATtcct                                                                          >  3:58674/1‑148 (MQ=255)                                                                       aaaaaaTGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACACCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATtcct                                                                          >  6:11075/1‑148 (MQ=255)                                                                        aaaaaTGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATtcctc                                                                         >  5:302988/1‑148 (MQ=255)                                                                             tGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCa                                                                    >  2:53269/1‑148 (MQ=255)                                                                               aCCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCAcc                                                                  <  4:169989/148‑1 (MQ=255)                                                                                 cGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCa                                                                                                              <  5:86805/102‑1 (MQ=255)                                                                                 cGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCa                                                                                                              >  6:86805/1‑102 (MQ=255)                                                                                   ggCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGc                                                              <  5:218292/148‑1 (MQ=255)                                                                                     cTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCtt                                                            >  3:106631/1‑148 (MQ=255)                                                                                       tGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTg                                                          >  3:312254/1‑148 (MQ=255)                                                                                        gAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCttt                                                           <  1:53269/146‑1 (MQ=255)                                                                                        acaagcgccaatgattggtcactaaattagcaacaagcgGAACATCACCTCGCACGGGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTGCGGCACCGGGGTTTGc                                                         <  5:175207‑M1/109‑1 (MQ=255)                                                                                         caagcgccaatgattggtcactaaattagcaacaagcgGAACATCACCTCGCACGGGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTa                                                                                  >  7:11659‑M1/39‑122 (MQ=255)                                                                                         caagcgccaatgattggtcactaaattagcaacaagcgGAACATCACCTCGCACGGGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTa                                                                                  <  8:11659‑M1/84‑1 (MQ=255)                                                                                         aaaGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGc                                                         <  8:80572/147‑1 (MQ=255)                                                                                         aaaGTCATGATGGCAAAAGCACAATAACGTGATTATGTGAACATCACCCCGGGCGAGGTGATGGTCCGCTTGTTGCTAA‑TTAGTGACCAATCATTGGCTCGTGGGGAATTTAGCGTCGGGACAATTCCTCCGGCACCGGGCTTTGcc                                                        <  6:311781/147‑1 (MQ=255)                                                                                            gTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATa                                                     >  5:301124/1‑148 (MQ=255)                                                                                            gTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATa                                                     >  8:346597/1‑148 (MQ=255)                                                                                             ttATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGa                                                                                                <  2:129295/104‑1 (MQ=255)                                                                                             ttATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGa                                                                                                >  1:129295/1‑104 (MQ=255)                                                                                                 gatgTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTccc                                                >  7:58613/1‑148 (MQ=255)                                                                                                    gTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGc                                              <  6:44856/147‑1 (MQ=255)                                                                                                    cacaaaatcacattATTTTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCa                                             >  3:308818/3‑148 (MQ=255)                                                                                                      caaaatcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATa                                           >  3:111614/1‑148 (MQ=255)                                                                                                        aaatcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACa                                         <  4:312254/148‑1 (MQ=255)                                                                                                          atcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACAtt                                       <  5:119832/148‑1 (MQ=255)                                                                                                           tcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTTCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTg                                      >  4:96411/1‑148 (MQ=255)                                                                                                           tcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTg                                      <  6:136961/148‑1 (MQ=255)                                                                                                                          aagcgGAACATCACCTCGCACGGGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTg                                      >  1:135504‑M1/6‑133 (MQ=255)                                                                                                                          aagcgGAACATCACCTCGCACGGGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTg                                      <  2:135504‑M1/128‑1 (MQ=255)                                                                                                                              tgAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTAGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCaa                    <  6:137745/147‑1 (MQ=255)                                                                                                                                  CATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATa               >  7:60589‑M1/1‑148 (MQ=255)                                                                                                                                    TCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAAt             >  1:202595‑M1/1‑148 (MQ=255)                                                                                                                                    TCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAAt             <  5:54725‑M1/148‑1 (MQ=255)                                                                                                                                       CCCCGTGCGAGGTGATGTTTCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATccc          >  3:96411‑M1/1‑148 (MQ=255)                                                                                                                                             gCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAAc    >  2:123034/1‑148 (MQ=255)                                                                                                                                             gCGAGGTGATGTACCGCTTGTTGCTAATTTAGTGACCAATCAGTGGCGCTTGTGGAATTAAGCGTCGGTCCAAATCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTACCCAAACACCGCAAACATAAACCCCCGAAc    >  2:126407/1‑148 (MQ=255)                                                                                                                                              cGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAAcc   <  2:83107/148‑1 (MQ=255)                                                                                                                                               gAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCa  <  4:106631/148‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                   GCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCA  >  NC_000913/1052121‑1052410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑