Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,539,455 G→A 5.8% Q1046* (CAG→TAG)  narZ ← nitrate reductase 2 (NRZ), alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,539,4550GA 5.8% 225.5 / 2.3 70Q1046* (CAG→TAG) narZnitrate reductase 2 (NRZ), alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (35/30);  new base A (2/2);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-01

ATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGC  >  NC_000913/1539308‑1539601
                                                                                                                                                   |                                                                                                                                                  
aTTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTg                                                                                                                                                    <  3:61789/148‑1 (MQ=255)
aTTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTg                                                                                                                                                    <  5:232463/148‑1 (MQ=255)
       gTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATaga                                                                                                                                               <  7:332131/146‑1 (MQ=255)
           ggcgTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAgctgct                                                                                                                                         <  1:313722/148‑1 (MQ=255)
           ggcgTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAgctgct                                                                                                                                         <  5:278250/148‑1 (MQ=255)
           ggcgTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTGTGGCGGTAGCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAgctgct                                                                                                                                         <  3:317115/148‑1 (MQ=255)
             cgTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTAATAGAGCTGCTgg                                                                                                                                       >  3:82017/1‑148 (MQ=255)
             cgTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTAATAGAGCTGCTgg                                                                                                                                       >  3:86962/1‑148 (MQ=255)
                   gAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTccc                                                                                                                                 >  8:82911/1‑148 (MQ=255)
                   gAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTccc                                                                                                                                 >  1:315591/1‑148 (MQ=255)
                      gTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGa                                                                                                                               >  6:188287/1‑147 (MQ=255)
                           gTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACa                                                                                                                             <  5:56904/144‑1 (MQ=255)
                           gTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACa                                                                                                                             >  6:56904/1‑144 (MQ=255)
                            tGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCTATAAGCCACCAGGCTTTCACCAAAAGCCCGAATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTg                                                                                                                        >  2:80411/1‑148 (MQ=255)
                                   tCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAATCACGCATCCACGGATGATCCTGATAGATCTGCTGGCTTCCCGACAGCGTTCGACACg                                                                                                                 >  8:380204/1‑148 (MQ=255)
                                     cGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAACACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGa                                                                                                               <  8:201195/148‑1 (MQ=255)
                                       ggAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAAt                                                                                                             >  8:307465/1‑148 (MQ=255)
                                         aaGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTAATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTa                                                                                                           <  4:82017/148‑1 (MQ=255)
                                         aaGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTAATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTa                                                                                                           <  4:86962/148‑1 (MQ=255)
                                            ccGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGTGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACt                                                                                                        <  4:146307/148‑1 (MQ=255)
                                              gTTTGGCGGTATCTGGCGCGTCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCa                                                                                                      <  4:213119/148‑1 (MQ=255)
                                                                cATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATccc                                                                                    >  6:356641/1‑148 (MQ=255)
                                                                   ctcACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCCCCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCcgcg                                                                                 >  2:11810/1‑148 (MQ=255)
                                                                   ctcACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCcgcg                                                                                 >  8:349863/1‑148 (MQ=255)
                                                                   ctcACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCcgcg                                                                                 >  2:319962/1‑148 (MQ=255)
                                                                   ctcACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCcgcg                                                                                 >  8:286187/1‑148 (MQ=255)
                                                                    tcACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAGTTAACTCATGAACGTTGGTGTATCCCGCGt                                                                                >  8:364458/1‑148 (MQ=255)
                                                                          aCGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAgg                                                                          <  7:82911/148‑1 (MQ=255)
                                                                              tACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAc                                                                      >  7:269167/1‑148 (MQ=255)
                                                                              tACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAc                                                                      >  4:247578/1‑148 (MQ=255)
                                                                               aCGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACg                                                                     >  6:198202/1‑148 (MQ=255)
                                                                                 gggTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATcc                                                                                     >  7:200244/1‑130 (MQ=255)
                                                                                 gggTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATcc                                                                                     <  8:200244/130‑1 (MQ=255)
                                                                                  ggTGTCGATAGTGTGGCGATAAGCCTCCAGGCTTTCACAAAAAGCACTCATCCACGTATTTTACTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCAACGTAATTAACTCATGAACGTTGGTGTATCCCGAGTTTTAGGAGACGTGa                                                                  <  1:177329/148‑1 (MQ=255)
                                                                                  ggTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGa                                                                  <  4:224177/148‑1 (MQ=255)
                                                                                     gtCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGCATTAACTCATGAACGTTGGTGGATCCCGCGTTATAGGAGCCGTGATCg                                                               >  5:247773/1‑148 (MQ=255)
                                                                                       cGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCt                                                             >  4:3043/1‑148 (MQ=255)
                                                                                       cGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCt                                                             <  2:290042/148‑1 (MQ=255)
                                                                                         aTAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCttt                                                           >  5:57686/1‑148 (MQ=255)
                                                                                         aTAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCttt                                                           <  5:356641/148‑1 (MQ=255)
                                                                                            ggcgggcgATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGTGCTGCTGGCGTCCCGACATCGTGCGCCACGGAATTAACTCATGAACGTTTGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTcc                                                         <  7:380204/147‑1 (MQ=255)
                                                                                            ggcgggcgATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAGTTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTcc                                                         <  7:364458/147‑1 (MQ=255)
                                                                                            ggcgggcgATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTcc                                                         <  5:285990/147‑1 (MQ=255)
                                                                                                ggcgATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGAt                                                                 >  4:93519/1‑135 (MQ=255)
                                                                                                ggcgATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGAt                                                                 <  3:93519/135‑1 (MQ=255)
                                                                                                  cgATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCgg                                                  <  8:269167/148‑1 (MQ=255)
                                                                                                   gATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAg                                                                           >  4:259856/1‑122 (MQ=255)
                                                                                                   gATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAg                                                                           <  3:259856/122‑1 (MQ=255)
                                                                                                     tAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGAc                                                >  3:223698/1‑147 (MQ=255)
                                                                                                       aGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTTATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCa                                              <  2:41171/147‑1 (MQ=255)
                                                                                                       aGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCa                                              <  3:3043/147‑1 (MQ=255)
                                                                                                        gCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAgg                                            >  1:190804/1‑148 (MQ=255)
                                                                                                         ccaccaGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGt                                           <  4:298204/148‑1 (MQ=255)
                                                                                                          caccaGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTg                                          <  7:286187/148‑1 (MQ=255)
                                                                                                           accaGGCTTTCACCAAAAGCACGCATCCACGGATGATACTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAAATAAATCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTgg                                         >  5:365859/1‑148 (MQ=255)
                                                                                                             caGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTgggg                                       <  3:247578/148‑1 (MQ=255)
                                                                                                              aGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGc                                      >  3:357212/1‑148 (MQ=255)
                                                                                                                       ccAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTggg                                        >  4:61814/1‑137 (MQ=255)
                                                                                                                       ccAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTggg                                        <  3:61814/137‑1 (MQ=255)
                                                                                                                        cAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAAt                            >  1:40818/1‑148 (MQ=255)
                                                                                                                                  cATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGa                                                 <  3:239415/117‑1 (MQ=255)
                                                                                                                                  cATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGa                                                 >  4:239415/1‑117 (MQ=255)
                                                                                                                                     ccACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTACAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTgc               >  2:253932/1‑148 (MQ=255)
                                                                                                                                     ccACGGATGATCCTGATAGAGCTGCTGGCGTCCCGAAAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGTCCGGACAAGGTGGGGCTGGAGTTTATTTTACGCGGCTgg               >  5:306316/1‑147 (MQ=255)
                                                                                                                                       aCGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTtata                                                                            <  6:204646/85‑1 (MQ=255)
                                                                                                                                       aCGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTtata                                                                            >  5:204646/1‑85 (MQ=255)
                                                                                                                                       aCGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTc                                                          >  3:38461/1‑103 (MQ=255)
                                                                                                                                       aCGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTc                                                          <  4:38461/103‑1 (MQ=255)
                                                                                                                                        cGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGt                                           >  7:211821/1‑117 (MQ=255)
                                                                                                                                        cGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGt                                           <  8:211821/117‑1 (MQ=255)
                                                                                                                                                 cTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATc    <  5:198202/147‑1 (MQ=255)
                                                                                                                                                  tGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTAGAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATcgc  >  1:229394/1‑148 (MQ=255)
                                                                                                                                                   gATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATcgc  >  7:120297/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGATGATCCTGATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGC  >  NC_000913/1539308‑1539601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑