Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,934,584 G→A 6.2% R7R (CGC→CGT edd ← 6‑phosphogluconate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,934,5840GA 6.2% 366.4 / 8.9 113R7R (CGC→CGTedd6‑phosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (56/50);  new base A (4/3);  total (60/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

Only 100 of 116 total aligned reads displayed.

TCTTCTGGCTGGCAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAA  >  NC_000913/1934438‑1934731
                                                                                                                                                  |                                                                                                                                                   
tcttctGTCTGTAAGGCAGCGAAACCGTGTGCCAGGTTACCTCATGCCAACTGCGAAAGATGAACGTTCGAAGTTTTCGCTTGTTCTATCAGGGCGAGATAAGCAGATCGAGTATCGCGAGAACGTTCAATGATTCGATTTGTTAcg                                                                                                                                                     <  8:122511/147‑1 (MQ=255)
tcttctGGCTGGCAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTAcg                                                                                                                                                     <  6:225761/147‑1 (MQ=255)
   tctGGCTGGCAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGt                                                                                                                                                  >  8:251101/1‑147 (MQ=255)
   tctGGCTGGCAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGt                                                                                                                                                  >  3:257607/1‑147 (MQ=255)
      ggctggcAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCTCGAACGTTCAATGATTCGATTTGTTACACGTAAc                                                                                                                                               >  7:309271/1‑147 (MQ=255)
             aggcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTgg                                                                                                                                       <  8:308641/148‑1 (MQ=255)
              ggcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGa                                                                                                                                      >  4:278793/1‑148 (MQ=255)
                  gCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCa                                                                                                                                  >  6:327690/1‑148 (MQ=255)
                   cGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCAt                                                                                                                                 <  3:310752/148‑1 (MQ=255)
                       aCCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAg                                                                                                                             >  2:10763/1‑148 (MQ=255)
                        ccGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAgg                                                                                                                            <  1:294977/148‑1 (MQ=255)
                            gtgCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTGTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCATTGATTCGATTTGTTACGCGTAACAATTGTGGATTCAGAAAGGCTcc                                                                                                                        >  5:81285/1‑148 (MQ=255)
                            gtgCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTcc                                                                                                                        <  2:82455/148‑1 (MQ=255)
                                 aGGTTACCGCATGCCAACTGTGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGaaa                                                                                                                   >  3:144117/1‑148 (MQ=255)
                                 aGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGaaa                                                                                                                   >  1:270295/1‑148 (MQ=255)
                                 aGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGaaa                                                                                                                   >  7:281015/1‑148 (MQ=255)
                                 aGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTAACGCGTAACAATTGTGGATTCATAAAGGCTCCTGaaa                                                                                                                   >  2:316946/1‑148 (MQ=255)
                                  ggTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAAt                                                                                                                  >  8:22900/1‑148 (MQ=255)
                                       ccGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAg                                                                                                              <  3:9436/147‑1 (MQ=255)
                                           aTGCCAAGTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGTTAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGATGTc                                                                                                         <  1:390195/148‑1 (MQ=255)
                                           aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTc                                                                                                         <  8:128484/148‑1 (MQ=255)
                                           aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTc                                                                                                         <  3:330211/148‑1 (MQ=255)
                                             gCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTCCGCGTACCAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGt                                                                                                          >  2:259430/1‑145 (MQ=255)
                                             gCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGt                                                                                                          >  6:292010/1‑145 (MQ=255)
                                             gCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGt                                                                                                          >  7:365445/1‑145 (MQ=255)
                                             gCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACACGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGt                                                                                                          >  1:224632/1‑145 (MQ=255)
                                                aaCTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCagag                                                                                                     >  1:325020/1‑147 (MQ=255)
                                                aaCTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCagag                                                                                                     >  5:344776/1‑147 (MQ=255)
                                                  cTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAg                                                                                                  >  6:313283/1‑148 (MQ=255)
                                                  cTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAg                                                                                                  >  3:94023/1‑148 (MQ=255)
                                                  cTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTATTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAg                                                                                                  >  3:26302/1‑148 (MQ=255)
                                                  cTGCGAACGATGAAAGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATTATTAGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGCAATTGAGTTGTCAGAGCAg                                                                                                  >  8:85899/1‑148 (MQ=255)
                                                   tGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGTTAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTTTCAGAGCa                                                                                                   <  3:90673/146‑1 (MQ=255)
                                                   tGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCa                                                                                                   >  4:90673/1‑146 (MQ=255)
                                                     cGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAgg                                                                                                 <  1:10763/146‑1 (MQ=255)
                                                      gAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGgatg                                                                                              <  5:292010/148‑1 (MQ=255)
                                                      gAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGgatg                                                                                              <  8:301867/148‑1 (MQ=255)
                                                       aaCGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGaa                                                                                                                    >  5:281364/1‑125 (MQ=255)
                                                        aCGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGgatgat                                                                                            <  1:380412/148‑1 (MQ=255)
                                                           aTGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATtg                                                                                                                                          >  2:254642/1‑99 (MQ=255)
                                                           aTGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATtg                                                                                                                                          <  1:254642/99‑1 (MQ=255)
                                                           aTGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTca                                                                                         >  4:208583/1‑148 (MQ=255)
                                                              aaCGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACaa                                                                                      <  4:315412/148‑1 (MQ=255)
                                                               aCGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACACGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAAc                                                                                     >  2:296776/1‑148 (MQ=255)
                                                                   tCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGt                                                                                 >  7:125678/1‑148 (MQ=255)
                                                                          tttCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGGGTATCAATTGTGGATTCATATAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcatt                                                                           >  1:208222/1‑147 (MQ=255)
                                                                          tttCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATACAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcatt                                                                           >  6:356515/1‑147 (MQ=255)
                                                                          tttCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCATAGCAGGATGATTCACAACGCGTttcatt                                                                           >  6:297800/1‑147 (MQ=255)
                                                                            tCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcatt                                                                           >  5:98420/1‑145 (MQ=255)
                                                                              gCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCaga                                                                       <  1:49271/147‑1 (MQ=255)
                                                                                 tcttCTATACGGGCGAGATAAGCTCAGCGATGCTCGCGCGAACGTTCAATGATTCGATTGGTTACGAGTAACAATTCTGGTTTCTTAAAGGCTCCTGAATTTGAGTTGACAGAGCAGGATGATTCACATCGCGTTTCATTCAGAggtt                                                                   <  2:244050/146‑3 (MQ=255)
                                                                                  gTTCTATCCGGGCGAGATAAGTAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGAtt                                                                  >  3:23330/1‑148 (MQ=255)
                                                                                  gTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGc                                                                                                    <  1:371805/114‑1 (MQ=255)
                                                                                  gTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGc                                                                                                    >  2:371805/1‑114 (MQ=255)
                                                                                  gTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTAACACGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGAt                                                                   <  1:296776/147‑1 (MQ=255)
                                                                                   ttCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGAttt                                                                 <  4:144117/148‑1 (MQ=255)
                                                                                   ttCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATGTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGAttt                                                                 <  5:119940/148‑1 (MQ=255)
                                                                                      tATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTAt                                                               >  4:123794/1‑147 (MQ=255)
                                                                                        tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCTCAACGCGTTTCATTCAGAGGATTTATg                                                              <  6:344776/146‑1 (MQ=255)
                                                                                        tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATg                                                              <  6:7493/146‑1 (MQ=255)
                                                                                        tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATg                                                              <  8:125678/146‑1 (MQ=255)
                                                                                          cGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTg                                                          <  4:94023/148‑1 (MQ=255)
                                                                                            ggCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCGTTCAGAGGATTTATGACTGaa                                                        <  2:325020/148‑1 (MQ=255)
                                                                                            ggCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGaa                                                        <  7:22900/148‑1 (MQ=255)
                                                                                                   tAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAACCGCCTg                                                 >  6:122809/1‑148 (MQ=255)
                                                                                                          agCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCg                                           >  7:318256/1‑147 (MQ=255)
                                                                                                             gAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGc                                       <  3:278793/148‑1 (MQ=255)
                                                                                                              aGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCt                                      <  2:141670/148‑1 (MQ=255)
                                                                                                               gTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCt                                      >  5:149238/1‑147 (MQ=255)
                                                                                                               gTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTc                                     <  1:89691/148‑1 (MQ=255)
                                                                                                                 ctcGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCa                                    >  3:142817/1‑147 (MQ=255)
                                                                                                                   cgcgcgAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAg                                 >  8:378179/1‑148 (MQ=255)
                                                                                                                   cgcgcgAACGTTAAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGAATGAAACGCCTGTAACCGGAGCTCATAg                                 >  6:169412/1‑148 (MQ=255)
                                                                                                                    gcgcgAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCAt                                   <  3:123794/145‑1 (MQ=255)
                                                                                                                    gcgcgAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCAt                                   <  5:327690/145‑1 (MQ=255)
                                                                                                                    gcgcgAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCAt                                   <  7:105178/145‑1 (MQ=255)
                                                                                                                    gcgcgAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAg                                 <  5:363794/147‑1 (MQ=255)
                                                                                                                    gcgcgAACGTTCAATGATTCGATTTGTCACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCAt                                   <  1:316946/145‑1 (MQ=255)
                                                                                                                    gagcgAACGTTCAATGATTCGATTTGTTACACGTTACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCAt                                   <  8:309271/143‑1 (MQ=255)
                                                                                                                     cgagAACGTTCAATGATTCGATTTATTACGAGTAACAATTGTGGATTAATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTTAAACGCCTGTAACCGGAGCTAATAggg                               <  4:26302/145‑1 (MQ=255)
                                                                                                                              tCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCTGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGATACGCCTGTAACCGGAGCTCATAGGGCAAAAACGt                      >  1:197379/1‑148 (MQ=255)
                                                                                                                              tCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGt                      >  3:3804/1‑148 (MQ=255)
                                                                                                                              tCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTAAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTAATAGGGCAAAAACGt                      >  7:333243/1‑148 (MQ=255)
                                                                                                                              tCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCATGAAATTGAGTTGTCAGAGCAGGATGATTCTCAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTACCCGGAGCTCATAGGGCAAAAACGt                      >  1:311633/1‑148 (MQ=255)
                                                                                                                                aaTGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGTCTCCTGAAATTTAGTTTTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATTACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGtt                     <  7:85899/147‑1 (MQ=255)
                                                                                                                                aaTGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGttt                    <  4:3804/148‑1 (MQ=255)
                                                                                                                                aaTGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGtt                     <  5:313283/147‑1 (MQ=255)
                                                                                                                                 aTGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGAttt                                                                 >  5:230204/1‑102 (MQ=255)
                                                                                                                                 aTGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGAttt                                                                 <  6:230204/102‑1 (MQ=255)
                                                                                                                                 aTGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGttt                    >  3:347849/1‑147 (MQ=255)
                                                                                                                                   gATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcat                                                                            <  7:143027/89‑1 (MQ=255)
                                                                                                                                   gATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcat                                                                            >  8:143027/1‑89 (MQ=255)
                                                                                                                                    aTTCGATTTGTTACGCGTAACTATTGTGGATTCATAAAGGCTCCTGATATTGAGTTGTCAGAGCAGGATGATTGACAACGCGTTTGATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagt                >  1:130993/1‑148 (MQ=255)
                                                                                                                                    aTTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcag                 <  7:251101/147‑1 (MQ=255)
                                                                                                                                       cGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagtc               <  2:375767/146‑1 (MQ=255)
                                                                                                                                        gATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTcagtcagt            <  7:378179/148‑1 (MQ=255)
                                                                                                                                         aTTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAgtg           >  3:182458/1‑148 (MQ=255)
                                                                                                                                            tGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGta         <  4:182631/147‑1 (MQ=255)
                                                                                                                                               tACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGtaata      >  4:160479/1‑147 (MQ=255)
                                                                                                                                                  gcgTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATaaaaa  <  8:333243/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TCTTCTGGCTGGCAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAA  >  NC_000913/1934438‑1934731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: