Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,968,056 G→A 6.6% A149V (GCG→GTG)  cheB ← fused chemotaxis regulator: protein‑glutamate methylesterase in two‑component regulatory system with CheA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,968,0560GA 6.6% 190.8 / 2.6 62A149V (GCG→GTG) cheBfused chemotaxis regulator: protein‑glutamate methylesterase in two‑component regulatory system with CheA
Reads supporting (aligned to +/- strand):  ref base G (26/30);  new base A (2/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

GAGCGGGTGAAACCGGGCGGCATATGCTGGGTAATTAACAGTGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGCATACCTTCGCGAATACCCAGTTGCGGTTTGGTGACAAAATCTAT  >  NC_000913/1967909‑1968198
                                                                                                                                                   |                                                                                                                                              
gagCGGGTGAAACCGGGCGGCATATGCTGGGTAATTAACAGTGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCg                                                                                                                                                <  7:236656/148‑1 (MQ=255)
     ggTGAACCCGGGCTGCATATGCTGGGTAATAAACAGTGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGACGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCTCCTTc                                                                                                                                           <  5:2729/148‑1 (MQ=255)
          aaCCGGGCGGCATATGCTGGGTAATTAACAGTGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGCCGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAAACAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGt                                                                                                                                      >  3:185783/1‑148 (MQ=255)
           aCCGGGCGGCATATGCTGGGTAATTAACAGTGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGtt                                                                                                                                     >  6:17560/1‑148 (MQ=255)
            ccGGGCGGCATATGCTGGGTAATTAACAGTGCCGGGCTGG‑AAGCCGCAAAGGTTGCAGTACGTGCCGAATTGCCTCAGTTCCACCCGTTGAAGCCCCAATCGCAATGCGTTTTTCAGAACTCAACCACGGCCCCCCCATCCGCGTTGt                                                                                                                                   >  6:126726/1‑148 (MQ=255)
             cGGGCGGCATATGCTGGGTAATTAACAGTGCCAGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGt                                                                                                                                   >  4:115010/1‑148 (MQ=255)
                     atatGCTGGGTAATTAACAGTGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCg                                                                                                                           <  5:140182/148‑1 (MQ=255)
                       atGCTGGGTAATTAACAGTGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCtt                                                                                                                                            >  8:85621/1‑129 (MQ=255)
                       atGCTGGGTAATTAACAGTGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCtt                                                                                                                                            >  8:80283/1‑129 (MQ=255)
                       atGCTGGGTAATTAACAGTGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCtt                                                                                                                                            <  7:85621/129‑1 (MQ=255)
                       atGCTGGGTAATTAACAGTGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCtt                                                                                                                                            <  7:80283/129‑1 (MQ=255)
                       atGCTGGGTAATTAACAGTGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGa                                                                                                                          >  6:120335/1‑147 (MQ=255)
                       atGCTGGGTAATTAACAGTGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGa                                                                                                                          >  4:193056/1‑147 (MQ=255)
                        tGCTGGGTAATTAACAGTGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACa                                                                                                                        <  7:14255/148‑1 (MQ=255)
                            gggTAATTAACAGTGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCCCCCGTTGAAGCCCCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATgg                                                                                                                    >  2:379144/1‑148 (MQ=255)
                              gTAATTAACAGTGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCt                                                                                                                  >  3:160446/1‑148 (MQ=255)
                                    tcccgggCCGGGCTGGTCTGGGGCATCGGGTGGAGTAAGTGACGAATCGCTTCAGTTCCACCCGATGCAGCACCAATCGCAAGCAGATTTTCAGAACTCAACAACGTCCCCGCCTTCCGGGGTGTCGCTGCCGACAATGGCTTAtgt                                                                                                             <  2:346103/141‑1 (MQ=255)
                                    aaCAGTGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTAtgt                                                                                                             <  1:213041/147‑1 (MQ=255)
                                     aCAGTGCCGGGCTGGAAAGCAGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTAtgtg                                                                                                            <  6:66511/147‑1 (MQ=255)
                                         tGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAAAAGCCTCAGTTCCACCCTTTGAAGCACAAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACa                                                                                                                        <  1:121517/131‑1 (MQ=255)
                                          gCCGTGCTGGAAAGCGGCAACGGTTGCAGTACGTGAAGAATTGCCTCAGTTCCACCCGTTGAAGCAACAATCGCAATCAGTTTTTCAGACCTAAACAACGGCCCCGCCTTGAGCGTTGTCGGTgacgacta                                                                                                                       >  2:121517/1‑129 (MQ=255)
                                           ccGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAg                                                                                                     <  5:17560/148‑1 (MQ=255)
                                              gtcTGGAATGCGGCAACGGTTGGAGTACGGGACGAATTGCCTCAGTTGCACCGGTTGAAGGACCTATCGCAATCAGTTTTTCAGAAGTCAACAACGGCACCGCCTTAAGCGTTTTCGGTGCCGACAATGGGTTATGTGATGCAAGGCt                                                                                                  <  8:288625/146‑1 (MQ=255)
                                              ggCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCt                                                                                                  <  4:257936/148‑1 (MQ=255)
                                                cTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCg                                                                                                <  4:185783/148‑1 (MQ=255)
                                                cTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCg                                                                                                <  5:331727/148‑1 (MQ=255)
                                                cTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCg                                                                                                <  3:198793/148‑1 (MQ=255)
                                                     aaGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCttt                                                                                           <  3:193056/148‑1 (MQ=255)
                                                             aCGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCg                                                                                    >  3:116508/1‑147 (MQ=255)
                                                              cGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTa                                                                                  >  6:3691/1‑148 (MQ=255)
                                                                    cAGTACGTGACGATTTGCCTCTGGTCCACCCGTTGAAGCACCATTCGCAATCTGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCGTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCAcc                                                                            <  1:87642/148‑1 (MQ=255)
                                                                    cAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCAcc                                                                            >  8:368437/1‑148 (MQ=255)
                                                                        aCGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCtttt                                                                        >  6:296450/1‑148 (MQ=255)
                                                                           tGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCtttt                                                                        >  3:234027/1‑145 (MQ=255)
                                                                                aaTTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCACCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCg                                                                                                <  8:247996/116‑1 (MQ=255)
                                                                                aaTTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCACCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCg                                                                                                <  8:242362/116‑1 (MQ=255)
                                                                                aaTTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCACCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCg                                                                                                >  7:247996/1‑116 (MQ=255)
                                                                                aaTTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCACCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCg                                                                                                >  7:242362/1‑116 (MQ=255)
                                                                                 aTTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCa                                                               >  7:257207/1‑148 (MQ=255)
                                                                                     ccTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCAttt                                                            <  5:223105/147‑1 (MQ=255)
                                                                                         aGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGtt                                                        <  5:120335/147‑1 (MQ=255)
                                                                                          gTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTtat                                                      <  7:368437/148‑1 (MQ=255)
                                                                                            tCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATAc                                                    >  8:231844/1‑148 (MQ=255)
                                                                                              cACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACg                                                   <  5:126726/147‑1 (MQ=255)
                                                                                                  cGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGc                                              <  4:116508/148‑1 (MQ=255)
                                                                                                       aaGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGCATAcc                                         <  8:257207/148‑1 (MQ=255)
                                                                                                           aCCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGCATACCTTcg                                     <  3:115010/148‑1 (MQ=255)
                                                                                                             cAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCAACTTTTCAGCAATCATTTCGTTATACGCCAGCATACCTTcgc                                    >  2:387501/1‑147 (MQ=255)
                                                                                                              aaTCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGCATACCTTcgcg                                   >  2:120935/1‑147 (MQ=255)
                                                                                                              aaTCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGCATACCTTCGCGa                                  <  7:372423/148‑1 (MQ=255)
                                                                                                                      tCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGCATACCTTCGCGAATACCCAg                          >  7:100020/1‑148 (MQ=255)
                                                                                                                           ttttCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGCATACCTTCGCGAATACCCa                           <  6:164274/142‑1 (MQ=255)
                                                                                                                           ttttCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGCATACCTTCGCGAATACCCa                           >  5:164274/1‑142 (MQ=255)
                                                                                                                                aGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGCATACCTTCGCGAATACCCAGTTGCGGttt                 <  4:234027/147‑1 (MQ=255)
                                                                                                                                      caacaaCGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGCATACCTTCGCGAATACCCAGTTGCGGTTTGGTGAc           >  3:351613/1‑147 (MQ=255)
                                                                                                                                       aacaacGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGCATACCTTCGCGAATACCCAGTTGCGGTTTGGTGACaa         <  1:8358/148‑1 (MQ=255)
                                                                                                                                         caacGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCAt                                                              <  7:301183/93‑1 (MQ=255)
                                                                                                                                         caacGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGCATACCTTCGCGAATACCCAGTTGCGGTTTGGTGACaaaa       <  1:379144/148‑1 (MQ=255)
                                                                                                                                         caacGGCCCCGCCTTCAGCGTTGACGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTGTTCAGCAATCAt                                                              >  8:301183/1‑93 (MQ=255)
                                                                                                                                          aacGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGCATACCTTCGCGAATACCCAGTTGCGGTTTGGTGACAAAAt      >  8:333372/1‑148 (MQ=255)
                                                                                                                                           acGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGCATACCTTCGCGAATACCCAGTTGCGGTTTGGTGACAAAATc     >  7:359661/1‑148 (MQ=255)
                                                                                                                                             ggCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGCATACCTTCGCGAATACCCAGTTGCGGTTTGGTGACAAAATCTa   >  6:269804/1‑148 (MQ=255)
                                                                                                                                              gCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGCATACCTTCGCGAATACCCAGTTGCAGTTTGGGGACAAAATCTAt  <  2:82193/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
GAGCGGGTGAAACCGGGCGGCATATGCTGGGTAATTAACAGTGCCGGGCTGGAAAGCGGCAACGGTTGCAGTACGTGACGAATTGCCTCAGTTCCACCCGTTGAAGCACCAATCGCAATCAGTTTTTCAGAACTCAACAACGGCCCCGCCTTCAGCGTTGTCGGTGCCGACAATGGCTTATGTGCTGCAAGGCTCGCCTTTGCTGCCGTACGCACCTTTTCAGCAATCATTTCGTTATACGCCAGCATACCTTCGCGAATACCCAGTTGCGGTTTGGTGACAAAATCTAT  >  NC_000913/1967909‑1968198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑