Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,982,787 | T→C | 6.2% | I252V (ATT→GTT) | araH ← | L‑arabinose ABC transporter permease |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,982,787 | 0 | T | C | 6.2% | 193.3 / 3.7 | 64 | I252V (ATT→GTT) | araH | L‑arabinose ABC transporter permease |
Reads supporting (aligned to +/- strand): ref base T (33/27); new base C (2/2); total (35/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.09e-01 |
AGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCC > NC_000913/1982641‑1982928 | aGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATc < 2:25963/148‑1 (MQ=255) agaAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGAc > 8:181296/1‑148 (MQ=255) gaAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAACCGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACg > 1:314549/1‑148 (MQ=255) aaTCTTAAGCTGGTTCATGGCGTTTTACACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCATGCGGAGATAACAATCAGCTCATAACCAATCGACGTc < 4:312342/148‑1 (MQ=255) atTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCAtt > 6:3284/1‑148 (MQ=255) cAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGc > 3:220316/1‑148 (MQ=255) cAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGATCATAACCAATCGACGTCATTGGCTGc > 2:368381/1‑148 (MQ=255) ggTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGccc < 3:362230/148‑1 (MQ=255) gTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCa < 7:366655/148‑1 (MQ=255) ttCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCAc > 2:153209/1‑148 (MQ=255) cATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACt < 7:181296/147‑1 (MQ=255) aTGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCAGCTTTGATATTTTTCCGTTGCCACCTTTCAGAGAAACGCCACCTAACACGCCGGCGGAGATAACAATCAGCTCATCAACAATCGACGGCATTTTCTCCCCACTgg < 2:252153/148‑1 (MQ=255) aTGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTgg < 6:62462/148‑1 (MQ=255) tGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGt < 3:28454/148‑1 (MQ=255) ggCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCTCATTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGt > 7:191143/1‑147 (MQ=255) gCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCa < 7:129932/148‑1 (MQ=255) tttCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACg > 8:26504/1‑148 (MQ=255) aCGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAg > 4:260984/1‑148 (MQ=255) aaaaTTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCAtt < 8:231419/114‑1 (MQ=255) aaaaTTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCAtt > 7:231419/1‑114 (MQ=255) aaaaTTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGaataa > 4:98864/1‑148 (MQ=255) aTTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTc < 3:260984/148‑1 (MQ=255) gATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGc > 6:86152/1‑147 (MQ=255) cGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCAAGAATAATTCCGGATATCGCt > 5:217216/1‑148 (MQ=255) gCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTg < 3:98864/148‑1 (MQ=255) caccacATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGa > 6:232915/1‑147 (MQ=255) cacaTATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATAcc < 5:3284/148‑1 (MQ=255) cacaTATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATAcc < 5:282392/148‑1 (MQ=255) acaTATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCACATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGCAGCCAGAATAATTCCGGCTATCGCTGATAc > 1:341743/1‑146 (MQ=255) tatGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGc > 6:5060/1‑148 (MQ=255) atGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGc < 4:249650/92‑1 (MQ=255) atGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGc > 3:249650/1‑92 (MQ=255) gagaTTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCt > 5:200264/1‑147 (MQ=255) gagaTTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAACCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCt > 7:104265/1‑147 (MQ=255) gagaTTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAACCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCt > 7:98880/1‑147 (MQ=255) gagaTTTTTCCGATACCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCt > 8:210369/1‑147 (MQ=255) ttttCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTgagag > 2:187679/1‑147 (MQ=255) cGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACaa < 1:153209/147‑1 (MQ=255) aTGCCACCTTTCAGAGAAACGCCACCTAAACCGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGa < 5:5060/148‑1 (MQ=255) aCCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATAcc < 7:293233/124‑1 (MQ=255) aCCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATAcc > 8:293233/1‑124 (MQ=255) ttCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAAttt > 5:245526/1‑148 (MQ=255) ttCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAAttt > 6:221062/1‑148 (MQ=255) agagAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAACCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTgg < 8:104265/148‑1 (MQ=255) agagAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAACCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTgg < 8:98880/148‑1 (MQ=255) agactaaCGCCACCTAAAACGCAGGAGGAGATATCACTCTGCTCATAAGCAATCGTCGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTgg < 2:314549/143‑1 (MQ=255) gagaAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGt < 4:220316/148‑1 (MQ=255) aCGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGaa < 5:86152/148‑1 (MQ=255) aaaaCGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACaa > 2:295882/1‑118 (MQ=255) aaaaCGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACaa < 1:295882/118‑1 (MQ=255) aaaaCGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGt > 5:173923/1‑147 (MQ=255) aaaaCGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTa > 4:200927/1‑148 (MQ=255) aaaCGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCgg < 6:173923/145‑1 (MQ=255) aaCGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGCTACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTaca > 5:93809/1‑148 (MQ=255) aaCGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAAGAAAGATAATTATGTTGGTGCGAACTAACGGTaca > 3:203598/1‑148 (MQ=255) aGGCGTAGATAACAATCTTCTCATCACCACTAGACGTAATTGGATGACCCCTTGTCATACGTGTAGCCAGATTAATTCAGGCTTTCTGTGATACCAGGCCTGAGAGAACAAATATAATAATTTTGTTGCGAACAAACGTTACACCCGc < 4:203598/148‑1 (MQ=255) cGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAg > 7:286270/1‑148 (MQ=255) aaTCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGcc < 6:200264/148‑1 (MQ=255) cAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTgagaga > 5:230191/1‑91 (MQ=255) cAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTgagaga < 6:230191/91‑1 (MQ=255) cAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCg < 2:280126/122‑1 (MQ=255) cAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCg > 1:280126/1‑122 (MQ=255) aaCCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTccc > 2:298085/1‑148 (MQ=255) aaCCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTccc > 1:325461/1‑148 (MQ=255) | AGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCC > NC_000913/1982641‑1982928 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |