Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,084,930 T→A 5.9% H199L (CAT→CTT)  yeeE ← UPF0394 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,084,9300TA 5.9% 191.4 / 2.4 67H199L (CAT→CTT) yeeEUPF0394 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (36/26);  new base A (2/2);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

ATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAA  >  NC_000913/2084783‑2085075
                                                                                                                                                   |                                                                                                                                                 
aTGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGAt                                                                                                                                                   <  6:86219/148‑1 (MQ=255)
           gTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGGTGAGTACAGCTGTGACAAAGGGATGCCAGCGtttt                                                                                                                                        >  1:363893/1‑148 (MQ=255)
                ccGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCaaa                                                                                                                                   <  5:53536/148‑1 (MQ=255)
                  gcgACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACa                                                                                                                                 <  3:14218/148‑1 (MQ=255)
                   cgACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCaaa                                                                                                                                   >  4:39745/1‑145 (MQ=255)
                   cgACCAGAAATGGCAGAATATTGGCCGTTGGGGAAGTGATTCCTACCCCACACATGCGTCCGGTTGCTTGACTCCGGGGCCAGGCTAAACGCGCAATCAACCCGATGAGTACAGCTGGGACAAAGGGATGCCAGGGTTTTTCAAACAg                                                                                                                                <  8:176354/148‑1 (MQ=255)
                     caaaGAAATTGCAGAATATTGGCCGGTGGGGAAGGGATACCTAAACAAAACATCCGTCCTTTTCCTTCACTCAGGCTCAAGCATAAAAGCGCAATCCAACCGATCAGTAAAGCTGACACAAAGCGATACCAGCCTTATACAAACAGa                                                                                                                               >  4:40553/4‑147 (MQ=255)
                      ccAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAat                                                                                                                             <  7:321404/148‑1 (MQ=255)
                         gAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAACCATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAatat                                                                                                                           >  1:311201/1‑147 (MQ=255)
                                cAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATc                                                                                                                   <  1:79371/148‑1 (MQ=255)
                                cAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATc                                                                                                                   <  1:257647/148‑1 (MQ=255)
                                   aaTATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACCTGCGTCCGGTGGCTTCACTCAGGGGCCAGTCTAAAAGCGCAATCAACCCGCTGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCg                                                                                                                <  6:294499/148‑1 (MQ=255)
                                   aaTATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGCGGCCAGGCTAAAAGCGAAATCAAACCGATTAGTACACCTGTGAAAAAGGGATGCCACCGATTTTAAAACAGAATATGAGCGATccag                                                                                                                >  6:71157/1‑146 (MQ=255)
                                     tatTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGGTGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGt                                                                                                              <  2:363893/148‑1 (MQ=255)
                                      atTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCATCACTCAGGGGCCAGGCTAAAAGCGCAATCAAAACGATTAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGtt                                                                                                             >  1:280941/1‑148 (MQ=255)
                                                 gggAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGcggcgg                                                                                                  >  7:34227/1‑148 (MQ=255)
                                                           tCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCTGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTcgc                                                                                         >  3:239302/1‑147 (MQ=255)
                                                               aaCCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCGAAAAGCGAAATCAATCCGATGAGTACTGCTGTGACAAAGGGATCCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGGTCGGCGCGGCGGAAAGCTCGCGACtt                                                                                    >  3:218999/1‑148 (MQ=255)
                                                                 cccAACCATGCGTCCGCTTGCATCCCTCCGGGGCCAGGCTAAAAGCGCAAGCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGGTTTTCAAACAGAATATGAGCGAGCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTg                                                                                  <  2:82842/148‑1 (MQ=255)
                                                                 cccAAACATGCGTCCGGTTGCTGCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTg                                                                                  <  8:365938/148‑1 (MQ=255)
                                                                  ccAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACa                                                                                                                                 >  1:361780/1‑100 (MQ=255)
                                                                  ccAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACa                                                                                                                                 <  2:361780/100‑1 (MQ=255)
                                                                   cAAACATGCGTCCGGTTGCTTCACTCATGGGCCAGGCTAAAAGAGCCATCAAACCGATGAGTACAGCTGTGACAAAGGGAAGCCAGCGTTTTTAAAACAGCAGATGAGCGATACAGGTTCTGCGCGGCTGTAAGGTCGAGAATTTGAg                                                                                >  6:322404/1‑148 (MQ=255)
                                                                   cAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCGAAAAGCTCAATCAAACCGATTAGTACAGCTGTGACAAAGGTAAGCCAGCGTTTTTCAAACAGAATATGAGCGCTCCCGGTTCGGAGAGGCGGTAAGGTCGCTACTTTGAg                                                                                >  6:316544/1‑148 (MQ=255)
                                                                    aaaCATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGa                                                                                 <  2:136119/146‑1 (MQ=255)
                                                                    aaaCATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGa                                                                                 >  1:136119/1‑146 (MQ=255)
                                                                      aCATGCGTGCGGTTGCTTCACTGAGGGGGCAGGCTAAAAGCGCAATGAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGATTATTAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGTCTTTGAGttt                                                                             <  5:71157/148‑1 (MQ=255)
                                                                      aCATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGttt                                                                             >  3:343299/1‑148 (MQ=255)
                                                                      aCATGCGACCGGGTGCTTCACTCAGGGGCCAGGCTACAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGGCGCGACTTTGAGttt                                                                             <  6:99284/148‑1 (MQ=255)
                                                                         tGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCCAACCTAGGAGTACCGCTGTGACAACGGGAAGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTgg                                                                          <  5:316544/148‑1 (MQ=255)
                                                                         tGCGTACGGTTGCTTCACTCAGTGGACAGTCTAAAATCGCAATCAAACCGATTAGTACAGCTGTGACAAAGTGAAGCCAGCGTTTTTCAAACAGAATATGATCGATCCCGGTTCGGCGCGGATGTAAGGTCGCGACTTTGAGTTTTgg                                                                          <  5:322404/148‑1 (MQ=255)
                                                                           cGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGct                                                                        <  8:82341/148‑1 (MQ=255)
                                                                            gTCCGGTTGCTTCACTCAGGGGCAAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATTAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGctt                                                                       <  2:311201/148‑1 (MQ=255)
                                                                                ggTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGcttctt                                                                    >  2:186669/1‑147 (MQ=255)
                                                                                 gTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCa                                                                  <  8:114999/148‑1 (MQ=255)
                                                                                  ttGCTTGACTGAGGGGCCAGTCTAAAAGCGCATTCTAACGGTTGATTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACTGAATTTGATCTATCGAGGTTCGGCGCGGCGGTTAGTTCTCGACTTTGAGTTTTGGCTGATTCa                                                                  <  2:41738/147‑1 (MQ=255)
                                                                                        cACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCttt                                                           >  1:117540/1‑148 (MQ=255)
                                                                                        cACGCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGAGGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCttt                                                           <  5:196981/148‑1 (MQ=255)
                                                                                            cAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTtcat                                                        >  8:183477/1‑147 (MQ=255)
                                                                                            cAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCCGTAAGGTCGCGACTTTTAGTTTTGGCTTCGTCAACTCTTtcatc                                                       >  2:58891/1‑148 (MQ=255)
                                                                                              ggggCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAActct                                                             <  4:129630/140‑1 (MQ=255)
                                                                                              ggggCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATcac                                                     <  8:34227/148‑1 (MQ=255)
                                                                                              ggggCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATcac                                                     >  2:55957/1‑148 (MQ=255)
                                                                                              ggggCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGACCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTTGCTTCTTCAActct                                                             >  3:129630/1‑140 (MQ=255)
                                                                                                 gCCAGGCTAAATGCGCTATCAAACCGATGAGTACAGGGGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGATCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTtc                                                          <  7:280944/140‑1 (MQ=255)
                                                                                                  ccAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCaccacc                                                 >  3:177035/1‑148 (MQ=255)
                                                                                                  ccAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAAAATGAGCGATCACGGTTCGGCGCGGCGGTAAGGTCTCGACTGTCAGTTTTGGCTTATTCAACACTAtca                                                         >  8:280944/1‑140 (MQ=255)
                                                                                                    aGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCaccacc                                                 >  2:140954/1‑146 (MQ=255)
                                                                                                    aGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTCTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACATTGAGTATTGGCTTCATCAAATCTTTCATcaccgac                                                 >  4:259333/1‑143 (MQ=255)
                                                                                                       cTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTgg                                                                          <  2:60326/118‑1 (MQ=255)
                                                                                                       cTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTgg                                                                          >  1:60326/1‑118 (MQ=255)
                                                                                                           tcttccgaTCTAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGt                                         >  5:179729/8‑147 (MQ=255)
                                                                                                              cgcAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATc                                     <  4:363349/148‑1 (MQ=255)
                                                                                                                 aaTCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCAc                                   >  1:230161/1‑147 (MQ=255)
                                                                                                                  aTCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCAc                                   >  8:343337/1‑146 (MQ=255)
                                                                                                                   tCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAg                                >  4:32372/1‑148 (MQ=255)
                                                                                                                    cAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCa                                                                  <  7:292768/113‑1 (MQ=255)
                                                                                                                    cAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCa                                                                  >  8:292768/1‑113 (MQ=255)
                                                                                                                    cAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAg                                >  7:102309/1‑147 (MQ=255)
                                                                                                                    cAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAg                                >  2:234315/1‑147 (MQ=255)
                                                                                                                      aaCCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTATGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCCTTATCACCAGCa                              >  7:95735/1‑147 (MQ=255)
                                                                                                                       aCCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCaaa                            <  7:343337/148‑1 (MQ=255)
                                                                                                                        ccGATGAGTACAGATGTGACAAATGGATTCCAGCGTTTTTCAAACAGAATATGAGAGATCCCGGTTCGGCTCGTCGGTAATGACTCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACAACCCAGAGAGATATCACCAGCaaaa                           >  6:15743/1‑148 (MQ=255)
                                                                                                                                         gACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCca           >  2:124974/1‑147 (MQ=255)
                                                                                                                                         gACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCca           >  5:283491/1‑147 (MQ=255)
                                                                                                                                              aGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGa     >  2:326144/1‑148 (MQ=255)
                                                                                                                                                 gATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCACCGGCCACACAGACa   >  5:375479/1‑147 (MQ=255)
                                                                                                                                                 gATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACaa  <  1:186669/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAA  >  NC_000913/2084783‑2085075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑