Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,263,951 C→T 6.5% A30V (GCG→GTG)  setB → lactose/glucose efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,263,9510CT 6.5% 193.7 / 2.3 61A30V (GCG→GTG) setBlactose/glucose efflux system
Reads supporting (aligned to +/- strand):  ref base C (29/28);  new base T (2/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTATTTTCTGACAAGCAGCGTAAACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGC  >  NC_000913/2263805‑2264096
                                                                                                                                                  |                                                                                                                                                 
tttATTTTCTGACAAGCAGCGTAAACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCg                                                                                                                                                  <  7:94663/148‑1 (MQ=255)
   aTTTTCTGACAAGCAGCGTAAACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCggg                                                                                                                                                <  7:218481/147‑1 (MQ=255)
    ttatCTGACAAGCAGCGTAAACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCAAGCATAACTCCCCCCCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTACTCACCGGTATTGCGGgcg                                                                                                                                              <  8:354418/145‑1 (MQ=255)
              agcagcGTAAACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAc                                                                                                                                    <  4:131748/148‑1 (MQ=255)
              agcagcGTAAACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAc                                                                                                                                    >  3:130821/1‑148 (MQ=255)
                      aaaCTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACTTGACATCGACGGCGTTTTTAATCGTTGCGTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGacac                                                                                                                            >  3:209791/1‑148 (MQ=255)
                       aaCTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACt                                                                                                                           >  3:99018/1‑148 (MQ=255)
                             gcgTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTAt                                                                                                                     >  6:208640/1‑148 (MQ=255)
                                tcttcctcttccAGTGATCGCCCAGCATGCATAACTCCCCCGCAGTCTCCCGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTAtttt                                                                                                                  >  2:171225/1‑148 (MQ=255)
                                tcttcctcttccAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTAtttt                                                                                                                  <  4:277266/148‑1 (MQ=255)
                                tcttcctcttccAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTAtttt                                                                                                                  >  3:277266/1‑148 (MQ=255)
                                       cttccAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTAcc                                                                                                           >  8:126749/1‑148 (MQ=255)
                                          ccAGTGATCGACCAGCATGCCTAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACTTGACATCGACGGCGTTTTTAATCGTTGCGTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCg                                                                                                          <  4:209791/146‑1 (MQ=255)
                                           cAGTGATCGACCCGCATCGATAACTCCCCCGCAGTCTCCAGGGCGAAAGCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGAt                                                                                                        <  2:172668/147‑1 (MQ=255)
                                            aGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGGCTTTCTCACCGGTATTGTGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATg                                                                                                       >  8:372459/1‑147 (MQ=255)
                                            aGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATg                                                                                                       >  2:145302/1‑147 (MQ=255)
                                            aGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGa                                                                                                      <  7:355145/148‑1 (MQ=255)
                                            aGTGATCGACCAGCATGCATAACTCCCCCGCAGGCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGa                                                                                                      <  5:155548/148‑1 (MQ=255)
                                            aGTGATCGACCAGCATGCAAAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGTGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATg                                                                                                       >  8:377550/1‑147 (MQ=255)
                                             gTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGaa                                                                                                     <  5:349825/148‑1 (MQ=255)
                                              tGATCGACCAGCATGCATCACTCCCCCGCAGTCTCCAGCGCGAATGCGTGTGACCTGACCTCGCCGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAg                                                                                                    <  4:214110/148‑1 (MQ=255)
                                              tGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGCCGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAg                                                                                                    <  8:87215/148‑1 (MQ=255)
                                               gATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGAAACTCAGTATTTTTCTTACCGATGAAg                                                                                                    >  3:277934/1‑147 (MQ=255)
                                                aTCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTa                                                                                                  <  1:229183/148‑1 (MQ=255)
                                                   gACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACAt                                                                                               >  2:310228/1‑148 (MQ=255)
                                                      cAGCATGCATAACTCCGCCGCAGTCGCCAGCGCGAAATCGGTTGACCTTACCTAGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCTGTATTTTTCTTACCGATGAAGTACATGcc                                                                                            <  6:53044/148‑1 (MQ=255)
                                                            gcatAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGATGTACATGCCCGTCCg                                                                                      >  8:290454/1‑148 (MQ=255)
                                                            gcatAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCg                                                                                      >  2:203013/1‑148 (MQ=255)
                                                             catAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTAc                                                                                                 <  3:357311/136‑1 (MQ=255)
                                                             catAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTAc                                                                                                 >  4:357311/1‑136 (MQ=255)
                                                                aaCTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATg                                                                                                       <  8:257292/127‑1 (MQ=255)
                                                                aaCTCCCCAGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATg                                                                                                       >  7:257292/1‑127 (MQ=255)
                                                                 aCTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGAt                                                                                 >  8:145805/1‑148 (MQ=255)
                                                                    cccccGCCGGCTCCAGCCCGAACTCGGTTGACCTGACCTCGACGGCGATTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTCCCGATGAAGTACATGCCCGTCCGGCGAtggt                                                                              <  8:179285/148‑1 (MQ=255)
                                                                    cccccGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGAtggt                                                                              >  8:28185/1‑148 (MQ=255)
                                                                     ccccGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGAtgg                                                                               <  4:130821/146‑1 (MQ=255)
                                                                      cccGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGAtggtgg                                                                            >  6:5802/1‑148 (MQ=255)
                                                                       ccGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGTGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTggg                                                                           <  7:372459/148‑1 (MQ=255)
                                                                       ccGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGTGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTggg                                                                           <  7:377550/148‑1 (MQ=255)
                                                                            gTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttct                                                                      >  6:71143/1‑148 (MQ=255)
                                                                            gTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttct                                                                      <  7:145805/148‑1 (MQ=255)
                                                                             tctcCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttctt                                                                     <  1:310228/148‑1 (MQ=255)
                                                                                 cAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTcttctt                                                                  >  2:237953/1‑147 (MQ=255)
                                                                                        aaaTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCgg                                                            >  1:31067/1‑146 (MQ=255)
                                                                                           tCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAgcg                                                       <  7:28185/148‑1 (MQ=255)
                                                                                             gTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAgcgc                                                      <  4:99018/147‑1 (MQ=255)
                                                                                                      gacctCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTGTCATTgg                                             >  8:45705/1‑147 (MQ=255)
                                                                                                       acctCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTGTCATTggg                                            <  1:145302/147‑1 (MQ=255)
                                                                                                        cctCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAACCCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCCTCAGGCGATGGTTGGATTCGTCTTTACCGGCGGCGCTGGCAGTGGGAt                                          >  4:10098/1‑148 (MQ=255)
                                                                                                           cGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTGTCATTg                                              >  2:218674/1‑141 (MQ=255)
                                                                                                           cGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTGTCATTg                                              <  1:218674/141‑1 (MQ=255)
                                                                                                           cGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCt                                       <  5:71143/148‑1 (MQ=255)
                                                                                                           cGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCt                                       >  4:206995/1‑148 (MQ=255)
                                                                                                                gCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGt                                    >  2:343784/1‑146 (MQ=255)
                                                                                                                    ttttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGt                                >  1:72726/1‑146 (MQ=255)
                                                                                                                       tAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGt                            <  7:45705/147‑1 (MQ=255)
                                                                                                                              tGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGcc                    >  3:265445/1‑148 (MQ=255)
                                                                                                                                ccTTTCTCACCGGTATTGCGGGCGCTCTGCAAACGCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCg                   <  7:129661/147‑1 (MQ=255)
                                                                                                                                    tctcACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGgcgc              >  4:53199/1‑148 (MQ=255)
                                                                                                                                          cGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGAt        <  1:203013/148‑1 (MQ=255)
                                                                                                                                                tGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAgcgc  >  5:195126/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTTATTTTCTGACAAGCAGCGTAAACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGC  >  NC_000913/2263805‑2264096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑