Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,317,667 T→G 100% E737A (GAA→GCA)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,317,6670TG100.0% 219.5 / NA 60E737A (GAA→GCA) rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (29/31);  total (29/31)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAA  >  NC_000913/2317526‑2317809
                                                                                                                                             |                                                                                                                                              
cTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTAtg                                                                                                                                          >  3:95165/1‑148 (MQ=255)
     tACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTa                                                                                                                                     <  8:182402/148‑1 (MQ=255)
     tACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCACTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTa                                                                                                                                     <  3:97526/148‑1 (MQ=255)
         ggcgccgcTGGCGCTTTCGCCAGCGGAATACCAATATGGCGACGACAGAAGGGCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAacga                                                                                                                                 <  8:228782/141‑1 (MQ=255)
            tCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAAcgacga                                                                                                                              >  4:162123/1‑148 (MQ=255)
                 tGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcg                                                                                                                         >  7:141902/1‑148 (MQ=255)
                 tGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcg                                                                                                                         >  7:333445/1‑148 (MQ=255)
                 tGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCAGTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcg                                                                                                                         >  3:13105/1‑148 (MQ=255)
                  ggCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTCCCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcgc                                                                                                                        >  3:42968/1‑148 (MQ=255)
                   gcgcTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcgc                                                                                                                        <  2:197810/147‑1 (MQ=255)
                         tctcCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcgctgcgct                                                                                                                  <  5:323213/147‑1 (MQ=255)
                              aGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAAc                                                                                                            >  8:169700/1‑148 (MQ=255)
                              aGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAAc                                                                                                            >  7:258627/1‑148 (MQ=255)
                               gCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACt                                                                                                           <  3:205769/148‑1 (MQ=255)
                                 ggAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTgg                                                                                                         >  8:261275/1‑148 (MQ=255)
                                  gAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGt                                                                                                        <  5:131815/148‑1 (MQ=255)
                                  gAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGt                                                                                                        <  2:98723/148‑1 (MQ=255)
                                      aCCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTc                                                                                                     >  1:827/1‑147 (MQ=255)
                                      aCCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTc                                                                                                     >  4:170761/1‑147 (MQ=255)
                                         aaTATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAg                                                                                                  >  3:96943/1‑147 (MQ=255)
                                              ggCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTa                                                                                                                                     <  2:388348/107‑1 (MQ=255)
                                              ggCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTa                                                                                                                                     >  1:388348/1‑107 (MQ=255)
                                                  acgacAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACag                                                                                        <  3:314366/148‑1 (MQ=255)
                                                     acAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGc                                                                                     <  7:145273/148‑1 (MQ=255)
                                                      cAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCg                                                                                    <  8:258627/148‑1 (MQ=255)
                                                          aGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGc                                                                                <  8:333445/148‑1 (MQ=255)
                                                             tCACTACCGCTCTGCCCTGCCATTTTTTACTCGCTACCGCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGGATCGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCttt                                                                             >  1:180263/1‑148 (MQ=255)
                                                                   ccGCTCTGCCCTGCAATATATTACTCACTACCTCGTAAGTGATCAACACATCTTCGGGAGTCGGTGCCTGCACTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCTACGAATTGCgg                                                                         <  7:165404/146‑1 (MQ=255)
                                                                   ccGCTCTGCCCTG‑CATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGTAGGCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGa                                                                        <  8:111156/146‑1 (MQ=255)
                                                                                ccATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCcagcag                                                          <  3:162123/148‑1 (MQ=255)
                                                                                 cATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCagcagc                                                         >  1:226406/1‑148 (MQ=255)
                                                                                    tttttACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTT‑CAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGtt                                                      >  1:131107/1‑147 (MQ=255)
                                                                                            cACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGGATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACt                                               >  5:367639/1‑147 (MQ=255)
                                                                                             aCTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCa                                             <  4:95165/148‑1 (MQ=255)
                                                                                             aCTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTCTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCa                                             <  4:19535/148‑1 (MQ=255)
                                                                                                  cTCGTCAGTGATCTACACATCTTCGTGAGTCGGTTCCTGCCCTGCGTATGTTGTATCGTCGATGCCGCTGCGCTGCTTACTGGTTTCCAGGAACTGACTGAGCGACTCATTGCGGACCGCCAGCCAGCAGCGTTTACCTCTCAACCCt                                        <  1:281755/148‑1 (MQ=255)
                                                                                                     gTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTcc                                     <  8:147300/148‑1 (MQ=255)
                                                                                                     gTCAGTGATCAACACATCTTCGGGAGTCGGGTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTcc                                     <  8:142094/148‑1 (MQ=255)
                                                                                                          tGATCAACACATCTTCGGGAGTCGGTTGCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACCGCGAGACGCATTGCGGACCGCCCGCCCTCAGCGGTTACCACTCCACCCTTCCACGcc                                >  4:296285/1‑148 (MQ=255)
                                                                                                           gATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCt                               >  8:229830/1‑148 (MQ=255)
                                                                                                            aTCAACACATCTTCGTGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCtt                              <  7:81806/148‑1 (MQ=255)
                                                                                                             tCAACACATCTTCGGGAGTCTGTTCCTGCCCTGCGTATTTTGTAACGACGATGCCGCTGCGATGCAAACTGTGTTCCATGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCtt                              <  4:281317/147‑1 (MQ=255)
                                                                                                             tCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCttt                             >  7:359719/1‑148 (MQ=255)
                                                                                                               accacaTCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTCACGACGATGCCGCTGCGCTGCAAACTGGGTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCttttt                           <  8:160112/146‑1 (MQ=255)
                                                                                                                acacATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTTGTTTCCAGGAACTGACAGAGCGACGCATTGCGGa                                                                        >  3:266371/1‑102 (MQ=255)
                                                                                                                acacATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGa                                                                        <  4:266371/102‑1 (MQ=255)
                                                                                                                 cacaTCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGc                                                                                >  6:232725/1‑93 (MQ=255)
                                                                                                                 cacaTCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGc                                                                                <  5:232725/93‑1 (MQ=255)
                                                                                                                 cacaTCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGccagccag                                                             <  3:206590/112‑1 (MQ=255)
                                                                                                                 cacaTCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGccagccag                                                             >  4:206590/1‑112 (MQ=255)
                                                                                                                    aTCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGc                      <  3:170761/148‑1 (MQ=255)
                                                                                                                      cTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCCACCCTTCCACGCCTTTTTTCTGCgg                    >  3:338703/1‑148 (MQ=255)
                                                                                                                        tCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCggg                   >  2:31388/1‑147 (MQ=255)
                                                                                                                         cGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCg                     <  4:96943/144‑1 (MQ=255)
                                                                                                                            gAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTg              <  7:261275/148‑1 (MQ=255)
                                                                                                                                cGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAgcg          >  8:222892/1‑148 (MQ=255)
                                                                                                                                cGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAgcg          >  7:239490/1‑148 (MQ=255)
                                                                                                                                   ttCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGc                      >  6:353463/1‑133 (MQ=255)
                                                                                                                                   ttCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGc                      <  5:353463/133‑1 (MQ=255)
                                                                                                                                        gCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGGCAGCAGCGTTTACCACTCAACCCTTCCCCGCCTTTTTTCTGCGGGTACTGAGCGCAGTGCaa  >  2:267332/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                              
CTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAA  >  NC_000913/2317526‑2317809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑