Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,400,119 G→A 5.0% A16V (GCG→GTG)  nuoG ← NADH:ubiquinone oxidoreductase, chain G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,400,1190GA 5.0% 259.1 / 2.2 80A16V (GCG→GTG) nuoGNADH:ubiquinone oxidoreductase, chain G
Reads supporting (aligned to +/- strand):  ref base G (36/39);  new base A (2/2);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

CACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGG  >  NC_000913/2399974‑2400266
                                                                                                                                                 |                                                                                                                                                   
cACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCt                                                                                                                                                   >  3:20410/1‑148 (MQ=255)
   cAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCg                                                                                                                                                <  8:145271/148‑1 (MQ=255)
          ccACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGAcat                                                                                                                                         >  5:325310/1‑146 (MQ=255)
          ccACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCt                                                                                                                                         <  6:314887/148‑1 (MQ=255)
                 tgtCTTCCGCGTTTTGGTATTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGCCAAGCTTCCAGCAGGTTGTCCGCTCCGTTGCCCTCGTATTc                                                                                                                                  >  1:181327/1‑148 (MQ=255)
                   tCTTCCGCGTTTTGGTATTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCtt                                                                                                                                <  3:184142/148‑1 (MQ=255)
                   tCTTCCGCGTTTTGGTATTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCtt                                                                                                                                <  1:200859/148‑1 (MQ=255)
                   tCTTCCGCGTTTTGGTATTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCt                                                                                                                                 <  5:203342/147‑1 (MQ=255)
                   tCTTCCGCGTTTTGGTATTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCt                                                                                                                                 <  5:253169/147‑1 (MQ=255)
                    cTTCCGCGTTTTGGTATTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGCCAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCttt                                                                                                                               >  1:364551/1‑148 (MQ=255)
                      tCCGCGTTTTGGTATTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGc                                                                                                                             >  7:245583/1‑148 (MQ=255)
                       ccGCGTTTTGGTATTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCGAGCAAAAGTAAGGAATATCACGGCCCTGAGACCGACAAGCTTCCAGCAGGTTGTCGGCTCCGTTGACCTCGGATTCGTTGcc                                                                                                                            >  3:297052/1‑148 (MQ=255)
                        cgcgTTTTGGTATTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCg                                                                                                                           >  1:43770/1‑148 (MQ=255)
                          cgTTTTGGTATTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGAGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTc                                                                                                                         >  3:184992/1‑148 (MQ=255)
                             tttGGTATTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTa                                                                                                                       >  6:25677/1‑147 (MQ=255)
                                   aTTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAAt                                                                                                                >  3:72533/1‑148 (MQ=255)
                                    ttGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAAtt                                                                                                               <  5:111293/148‑1 (MQ=255)
                                         tCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCg                                                                                                                           <  1:327838/131‑1 (MQ=255)
                                         tCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCg                                                                                                                           >  2:327838/1‑131 (MQ=255)
                                         tCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGc                                                                                                          >  5:194569/1‑148 (MQ=255)
                                                acccTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTATGGAATATCAAGGCCCAGAGACAGACCAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGc                                                                                                   <  2:292094/145‑1 (MQ=255)
                                                acacTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGc                                                                                                   <  4:20410/148‑1 (MQ=255)
                                                acacTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGc                                                                                                   <  3:79534/148‑1 (MQ=255)
                                                acacTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGc                                                                                                   <  8:343534/148‑1 (MQ=255)
                                                     ggcggcAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGt                                                                                                                                               >  1:315048/1‑99 (MQ=255)
                                                     ggcggcAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGt                                                                                                                                               <  2:315048/99‑1 (MQ=255)
                                                     ggcggcAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTg                                                                                                              <  7:357442/132‑1 (MQ=255)
                                                     ggcggcAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCAGTTGACCTCGTATTCTTTGCCGTCTACATGAATTg                                                                                                              >  8:357442/1‑132 (MQ=255)
                                                        ggcAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTcc                                                                                           <  4:87635/148‑1 (MQ=255)
                                                          cAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAg                                                                                         >  5:283328/1‑148 (MQ=255)
                                                           aaGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCAt                                                                                                       >  4:186778/1‑133 (MQ=255)
                                                           aaGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCAt                                                                                                       <  3:186778/133‑1 (MQ=255)
                                                            aGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCt                                                                                                                                                   >  1:209954/1‑88 (MQ=255)
                                                            aGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCt                                                                                                                                                   <  2:209954/88‑1 (MQ=255)
                                                            aGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGtt                                                                                       >  6:236317/1‑148 (MQ=255)
                                                              cACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGtttt                                                                                     >  2:208131/1‑148 (MQ=255)
                                                                 cGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCTAGGCCCAGAGACAGACAAGCTTCCATCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTtctc                                                                                  <  3:157788/148‑1 (MQ=255)
                                                                 cGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACACGCAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTtctc                                                                                  <  5:275917/148‑1 (MQ=255)
                                                                  gACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTtct                                                                                   >  6:175570/1‑146 (MQ=255)
                                                                  gACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCACTCCGTTGCCCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTtct                                                                                   >  7:61715/1‑146 (MQ=255)
                                                                  gACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCACTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTtct                                                                                   >  7:55568/1‑146 (MQ=255)
                                                                     acTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCtcagtc                                                                              <  2:43770/148‑1 (MQ=255)
                                                                        tCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCaga                                                                           >  7:207172/1‑148 (MQ=255)
                                                                             gcgcCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACt                                                                       >  6:284173/1‑147 (MQ=255)
                                                                                         aGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCg                                                           >  6:272396/1‑147 (MQ=255)
                                                                                              aaGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTc                                                     <  6:283328/148‑1 (MQ=255)
                                                                                                gTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCgg                                                   <  5:321213/148‑1 (MQ=255)
                                                                                                   aGGACTATCTACGCCCAGAGACAGACAACCTTCCACCAGGTAGTCCGCTCCGTTGACCTCGAATTCTTTGCCCGCTACATGAAATGTAGCCATTAGCATGCTTCCAGTTTTCTCAGGCAGAGACTGAGCGTTAATCGAAATTCGGTTa                                                <  4:297052/148‑1 (MQ=255)
                                                                                                         atCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAgcg                                          >  3:160701/1‑148 (MQ=255)
                                                                                                         atCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAgcg                                          >  5:250376/1‑148 (MQ=255)
                                                                                                         atCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTCACCTCGTATTCATTCCCGACTACATGAAAAGTAGCAATTAGCATGCTTCCAGTATTCTCAGAAAGAGACTGAGCGTTAATCTAAATACGGTTACCAgag                                          >  4:335972/1‑146 (MQ=255)
                                                                                                              ggCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCtt                                     <  5:25677/148‑1 (MQ=255)
                                                                                                               gCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCttt                                    <  5:272396/148‑1 (MQ=255)
                                                                                                                  cAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGCATTCTTTGCCGTCTACATGAAATGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTcag                                 >  6:210442/1‑148 (MQ=255)
                                                                                                                   agagACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTcagc                                <  4:160701/148‑1 (MQ=255)
                                                                                                                    gagaCAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTc                                                     >  6:102812/1‑126 (MQ=255)
                                                                                                                    gagaCAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTc                                                     <  5:102812/126‑1 (MQ=255)
                                                                                                                    gagaCAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTcagca                               <  5:236317/148‑1 (MQ=255)
                                                                                                                    gagaCAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTcagca                               <  5:175570/148‑1 (MQ=255)
                                                                                                                    gagaCAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTcag                                 >  8:252416/1‑146 (MQ=255)
                                                                                                                    gagaCAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTcag                                 >  2:363933/1‑146 (MQ=255)
                                                                                                                    gagaCAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTcag                                 >  1:112249/1‑146 (MQ=255)
                                                                                                                          gacaAGCTTCCAGCAGGTTGTACTCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGACATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGAGCTCTTTCAGCAGGTTCg                         <  8:349120/148‑1 (MQ=255)
                                                                                                                            caAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTa                                                >  1:292735/1‑123 (MQ=255)
                                                                                                                            caAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTa                                                <  2:292735/123‑1 (MQ=255)
                                                                                                                               gCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGa                    <  5:284173/148‑1 (MQ=255)
                                                                                                                               gCTTCCAGCAGGTTGTCCACTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGa                    <  8:55568/148‑1 (MQ=255)
                                                                                                                               gCTTCCAGCAGGTTGTCCACTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGa                    <  8:61715/148‑1 (MQ=255)
                                                                                                                                  tCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGCCTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATc                 >  2:107318/1‑148 (MQ=255)
                                                                                                                                  tCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCgg                                                   >  8:111250/1‑114 (MQ=255)
                                                                                                                                  tCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGATTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCgg                                                   <  7:111250/114‑1 (MQ=255)
                                                                                                                                   ccAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATc                 <  7:252416/147‑1 (MQ=255)
                                                                                                                                    cagcagGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATccc               <  8:245583/148‑1 (MQ=255)
                                                                                                                                     agcagGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCa              >  1:59373/1‑148 (MQ=255)
                                                                                                                                        agGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCAtt            >  1:138145/1‑147 (MQ=255)
                                                                                                                                              tCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCaa      <  6:250376/147‑1 (MQ=255)
                                                                                                                                              tCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCaa      <  3:335972/147‑1 (MQ=255)
                                                                                                                                              tCCGCTCCGTTGACCTCGTAGTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTCCCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCaa      <  4:184992/147‑1 (MQ=255)
                                                                                                                                              tCCGCGCCGCTGACCTCGTATTCTTTGCCGGCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGGAAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCaa      <  8:278789/147‑1 (MQ=255)
                                                                                                                                                cGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAAt    <  2:364551/147‑1 (MQ=255)
                                                                                                                                                 gCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTa           >  6:348847/1‑139 (MQ=255)
                                                                                                                                                 gCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTa           <  5:348847/139‑1 (MQ=255)
                                                                                                                                                 gCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATgg  <  8:207172/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGCACACTGGCGGCAAGCACCGACACTTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGG  >  NC_000913/2399974‑2400266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: