Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,510,349 C→T 5.4% A240A (GCC→GCT)  yfeO → putative ion channel protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,510,3490CT 5.4% 243.7 / 2.2 75A240A (GCC→GCT) yfeOputative ion channel protein
Reads supporting (aligned to +/- strand):  ref base C (28/42);  new base T (2/2);  total (31/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

GGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAG  >  NC_000913/2510202‑2510487
                                                                                                                                                   |                                                                                                                                          
ggcggcAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGcc                                                                                                                                            >  4:238397/1‑148 (MQ=255)
  cggcAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGt                                                                                                                                          <  5:371790/148‑1 (MQ=255)
  cggcAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGt                                                                                                                                          <  2:6673/148‑1 (MQ=255)
     cagcagCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATg                                                                                                                                       <  3:238397/148‑1 (MQ=255)
           cTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCtt                                                                                                                                 <  1:238517/148‑1 (MQ=255)
              gTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTAcc                                                                                                                              <  3:56718/148‑1 (MQ=255)
                    ttACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGtt                                                                                                                        >  1:189959/1‑148 (MQ=255)
                     tactacCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTg                                                                                                                       >  1:96444/1‑148 (MQ=255)
                         acCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCAcg                                                                                                                   >  2:157836/1‑148 (MQ=255)
                           cGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCTCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCAcgcg                                                                                                                 <  5:122783/148‑1 (MQ=255)
                           cGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCAcgc                                                                                                                  >  8:133592/1‑147 (MQ=255)
                             gATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCgat                                                                                                               <  6:16254/148‑1 (MQ=255)
                              attattTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCgatg                                                                                                              <  8:198675/148‑1 (MQ=255)
                              attattTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCgatg                                                                                                              <  2:96444/148‑1 (MQ=255)
                              attattTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGGCGCCGTATGGTGCTTACCACGGTTGCACGCgatg                                                                                                              <  6:286481/148‑1 (MQ=255)
                              attattTTTCCATCCTCAATTTTAACTGCCCATTGCTCATTACGGAAAGATGGAAATGACCGATATTATCAGAGCTCCAATACTCCCGCCAATTGACAACTCAACAGGGATGTTCGCAGTATGGTGCTTAACACGGTTGcgaacgat                                                                                                               >  4:303354/1‑140 (MQ=255)
                               ttattTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCgatga                                                                                                             <  7:26683/148‑1 (MQ=255)
                               ttattTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCgatga                                                                                                             <  7:208477/148‑1 (MQ=255)
                                 attTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGatgatg                                                                                                           >  8:228511/1‑148 (MQ=255)
                                  tttttCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGatgatg                                                                                                           <  7:133592/147‑1 (MQ=255)
                                      tCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCCCGCGATGATGCATc                                                                                                       >  1:250433/1‑147 (MQ=255)
                                        cATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCaa                                                                                                     <  2:250433/147‑1 (MQ=255)
                                          tCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAAt                                                                                                   >  3:339811/1‑147 (MQ=255)
                                           ccTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATTACCGATATTCTAAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATg                                                                                                  >  7:354234/1‑147 (MQ=255)
                                           ccTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATg                                                                                                  >  6:375609/1‑147 (MQ=255)
                                              cATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGaaa                                                                                               >  6:358314/1‑147 (MQ=255)
                                              cATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGaaa                                                                                               >  7:305541/1‑147 (MQ=255)
                                                tttttCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAAt                                                                                            <  7:228511/148‑1 (MQ=255)
                                                    tCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCg                                                                                         <  5:358314/147‑1 (MQ=255)
                                                      aCTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTg                                                                                      >  5:116529/1‑148 (MQ=255)
                                                        tGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCt                                                                                    >  7:152743/1‑148 (MQ=255)
                                                        tGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCt                                                                                    >  4:185961/1‑148 (MQ=255)
                                                             aTTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGc                                                                               <  1:135864/148‑1 (MQ=255)
                                                               tGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCgatg                                                                                                              >  8:56831/1‑115 (MQ=255)
                                                               tGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCgatg                                                                                                              <  7:56831/115‑1 (MQ=255)
                                                               tGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTg                                                                             >  2:363458/1‑148 (MQ=255)
                                                                   cATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCTGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTa                                                                         >  6:104239/1‑148 (MQ=255)
                                                                   cATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCTGTATGGTGCTTAACACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGTTGCTCGTGCTGGGTa                                                                         >  6:98990/1‑148 (MQ=255)
                                                                    aTTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGt                                                                          <  6:227634/146‑1 (MQ=255)
                                                                     ttACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTAtt                                                                       <  4:339811/148‑1 (MQ=255)
                                                                      tACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTTCCACGGTTGCACGCGATGATGCAGCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTg                                                                      >  6:324551/1‑148 (MQ=255)
                                                                      tACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGa                                                                                                 <  7:167720/121‑1 (MQ=255)
                                                                      tACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGa                                                                                                 >  8:167720/1‑121 (MQ=255)
                                                                        cGGACAGTTGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTgg                                                                     <  8:206265/147‑1 (MQ=255)
                                                                        cGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTgg                                                                     <  3:348998/147‑1 (MQ=255)
                                                                           aCAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGa                                                                 <  3:76742/148‑1 (MQ=255)
                                                                                tGGAAATGACCGATATTCTCAGCGGTGCAATAGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTAGCACGCGATGATGCATCAAATGAAACATCCGGTGGGCGGGCTGGGTATTGGCGGAGTTAt                                                            >  8:159597/1‑148 (MQ=255)
                                                                                       gACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGc                                                                                                          <  7:237425/95‑1 (MQ=255)
                                                                                       gACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGc                                                                                                          >  8:237425/1‑95 (MQ=255)
                                                                                        aCCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGt                                                     <  3:303354/147‑1 (MQ=255)
                                                                                         ccGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCTGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTAt                                                   <  5:98990/148‑1 (MQ=255)
                                                                                         ccGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCTGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTAt                                                   <  5:104239/148‑1 (MQ=255)
                                                                                           gATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTc                                                 >  1:31680/1‑148 (MQ=255)
                                                                                             tatTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTg                                               <  7:30689/148‑1 (MQ=255)
                                                                                               ttCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTggg                                             <  1:157836/148‑1 (MQ=255)
                                                                                                 ctcAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTgggg                                            <  8:152743/147‑1 (MQ=255)
                                                                                                   cAGCGGTGCAATTGTCGCGGCGATTGCCATCGCCGCAGGGATTGTCGCCGTATGGTGCTTACCACGGTTGCACCCGCTGATGCATCAAATGAACAAGCCGGTGCTAGTGCTTGGTATTGGCGGATTTAGTCTCGGTATTC‑GGGGGtt                                         >  5:25663/1‑147 (MQ=255)
                                                                                                   cAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGt                                          >  8:285865/1‑147 (MQ=255)
                                                                                                    aGCGTTGCAATTGTCGCGGCGATTTCCATCGCAGCAGGGATTGTCGCCGTATGGTGCTTACCACGGTTGCACGCTATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGtt                                         <  5:44986/147‑1 (MQ=255)
                                                                                                       ggTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATtg                                     <  1:363458/148‑1 (MQ=255)
                                                                                                         tGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATtggt                                   >  8:124099/1‑148 (MQ=255)
                                                                                                             aTTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGAc                               >  3:4351/1‑148 (MQ=255)
                                                                                                               tGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAAt                                                                                            >  5:160173/1‑85 (MQ=255)
                                                                                                               tGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAAt                                                                                            <  6:160173/85‑1 (MQ=255)
                                                                                                                     ggcgATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCg                       >  7:158919/1‑148 (MQ=255)
                                                                                                                      gcgATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGc                      <  2:31680/148‑1 (MQ=255)
                                                                                                                      gcgATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGc                      <  5:375609/148‑1 (MQ=255)
                                                                                                                             ccATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGTTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTaa               <  4:92446/148‑1 (MQ=255)
                                                                                                                             ccATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTa                <  8:354234/147‑1 (MQ=255)
                                                                                                                             ccATCGCAGCAGGGATGGGCGCCGGATGGTGCTTACCACGGTTGCACGCGATGCTGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTaa               <  2:277988/148‑1 (MQ=255)
                                                                                                                               aTCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGATTATTGGTGGACCAGTTTCGCTGTTTAAAg             <  8:305541/148‑1 (MQ=255)
                                                                                                                               aTCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGAGGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAg             <  2:131888/148‑1 (MQ=255)
                                                                                                                                     gcagGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGGTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTgg       <  2:189959/148‑1 (MQ=255)
                                                                                                                                          gATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTAt                                                                        <  8:163784/78‑1 (MQ=255)
                                                                                                                                          gATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTAt                                                                        >  7:163784/1‑78 (MQ=255)
                                                                                                                                          gATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATgag  <  6:116529/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                          
GGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAG  >  NC_000913/2510202‑2510487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑