Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,512,378 A→G 5.3% L110P (CTG→CCG)  mntH ← manganese/divalent cation transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,512,3780AG 5.3% 230.0 / 2.6 75L110P (CTG→CCG) mntHmanganese/divalent cation transporter
Reads supporting (aligned to +/- strand):  ref base A (33/37);  new base G (2/2);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATC  >  NC_000913/2512231‑2512524
                                                                                                                                                   |                                                                                                                                                  
ccGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCa                                                                                                                                                    <  8:121557/148‑1 (MQ=255)
 cGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGATAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAg                                                                                                                                                   >  6:229134/1‑148 (MQ=255)
  gCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAgg                                                                                                                                                  >  7:214407/1‑148 (MQ=255)
       tCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCa                                                                                                                                                    >  6:73612/1‑141 (MQ=255)
       tCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCa                                                                                                                                                    <  5:73612/141‑1 (MQ=255)
        cACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAgg                                                                                                                                                  >  1:317007/1‑142 (MQ=255)
        cACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAgg                                                                                                                                                  <  2:317007/142‑1 (MQ=255)
            ttCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGcc                                                                                                                                         >  6:91941/1‑147 (MQ=255)
            ttCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCa                                                                                                                                        >  4:102110/1‑148 (MQ=255)
             tctcCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGcc                                                                                                                                         <  8:214407/146‑1 (MQ=255)
             tctcCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCa                                                                                                                                        <  1:159613/147‑1 (MQ=255)
             tctcCAGCGGTGTTTGCCCGCGACGTTGCACCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTCAACCCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGcc                                                                                                                                         <  8:383719/146‑1 (MQ=255)
                ccAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCg                                                                                                                                     >  5:140704/1‑147 (MQ=255)
                 cAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATc                                                                                                                                      <  4:272443/145‑1 (MQ=255)
                         tttGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCGCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTc                                                                                                                           >  4:332705/1‑148 (MQ=255)
                               cgcgACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCACCAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCGGGTCGGTTGCCATCGCAATAATTTCTGCCt                                                                                                                      >  2:349744/1‑147 (MQ=255)
                                     gTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCa                                                                                                               <  4:170710/148‑1 (MQ=255)
                                      ttGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCaa                                                                                                              >  7:355181/1‑148 (MQ=255)
                                       tGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACGCaa                                                                                                              >  2:140193/1‑147 (MQ=255)
                                         cagcaTTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCCCCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGGCTGAACCCaa                                                                                                              >  5:22025/1‑145 (MQ=255)
                                          agcaTTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAg                                                                                                           <  1:343785/147‑1 (MQ=255)
                                           gcaTTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGaa                                                                                                         <  2:237578/148‑1 (MQ=255)
                                           gcaTTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGaa                                                                                                         <  5:312890/148‑1 (MQ=255)
                                           gcaTTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGaa                                                                                                         <  5:145123/148‑1 (MQ=255)
                                                    tCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTAc                                                                                                <  2:182292/148‑1 (MQ=255)
                                                    tCAGGAAAGGCGCGATCCCCGTCAGCACCGCGCCCGGCAACAACGACACACCAAGAATGAGTTTAAAACCGAGCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTAc                                                                                                <  2:156997/148‑1 (MQ=255)
                                                       ggAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAACCCGATCGCCGCGCCTATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTCCCTGACCCCAATAGACCCACACAACgg                                                                                              >  5:360917/1‑147 (MQ=255)
                                                        gAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGa                                                                                            >  3:220386/1‑148 (MQ=255)
                                                          aaGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGCACCCAATAGAACCACACTACGGGAcg                                                                                          >  2:372531/1‑148 (MQ=255)
                                                            gTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACAGCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGTCTGAACCCAATAGAACCACACTACGGGAcgcg                                                                                        <  5:333162/148‑1 (MQ=255)
                                                             tCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGAcgcg                                                                                        >  2:81384/1‑147 (MQ=255)
                                                                cgATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGa                                                                                      >  7:47443/1‑146 (MQ=255)
                                                                       cGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATcg                                                                             >  3:272702/1‑148 (MQ=255)
                                                                       cGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATcg                                                                             <  2:11667/148‑1 (MQ=255)
                                                                        gTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATcgc                                                                            >  5:357998/1‑148 (MQ=255)
                                                                          ctgCACCGCGCCCAGCAACAACGCAACACCAAGAATGAGAATAAACCCCCTCGCCCCACCAATAAAATCCGCCAGGTCGGTTGCCATCGAAATAATTTCAGCCAGAACCCAATTCATCCACACTACGCGACGCGGATAGTGATcgag                                                                           >  6:107947/3‑145 (MQ=255)
                                                                          cAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTAAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATcgcg                                                                           >  4:340233/1‑147 (MQ=255)
                                                                           aGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACAGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCTTCTCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGGTCGCGaa                                                                         <  5:91941/148‑1 (MQ=255)
                                                                           aGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTAAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGa                                                                          <  3:340233/147‑1 (MQ=255)
                                                                            gCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCGGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAAt                                                                        >  4:130412/1‑148 (MQ=255)
                                                                                 gcgcCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACACAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCt                                                                   >  8:1809/1‑148 (MQ=255)
                                                                                  cgcCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGt                                                                                  <  5:199809/132‑1 (MQ=255)
                                                                                  cgcCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGt                                                                                  >  6:199809/1‑132 (MQ=255)
                                                                                        gCAACAACGAAACACCAAGAATGAGTTTAAAACCGCTCGACGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGAGAATCTGCTCCGc                                                               >  3:260713/1‑145 (MQ=255)
                                                                                        gCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACcaca                                                                                                    >  1:13854/1‑108 (MQ=255)
                                                                                        gCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGc                                                               >  8:326077/1‑145 (MQ=255)
                                                                                        gCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGc                                                               >  1:168808/1‑145 (MQ=255)
                                                                                        gCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGc                                                               >  2:983/1‑145 (MQ=255)
                                                                                        gCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCAAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACcaca                                                                                                    <  2:13854/108‑1 (MQ=255)
                                                                                            caacGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGaa                                                                         >  5:215469/1‑131 (MQ=255)
                                                                                            caacGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGaa                                                                         <  6:215469/131‑1 (MQ=255)
                                                                                              acGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGAttttt                                                      <  6:357998/148‑1 (MQ=255)
                                                                                               cGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCGGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTa                                                     <  3:130412/148‑1 (MQ=255)
                                                                                               cGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTa                                                     <  5:229134/148‑1 (MQ=255)
                                                                                                 aaaCACCAAGAATGAGTTTAAAACCGAGCGCCGCCCCAATAAATTCCGCCCGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCTAATCTGCTCCGCCAGATTTTTAcc                                                   <  6:242499/148‑1 (MQ=255)
                                                                                                  aaCACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCg                                                  <  3:298180/148‑1 (MQ=255)
                                                                                                    cacCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGt                                                <  3:102110/148‑1 (MQ=255)
                                                                                                      ccAAGAATGATGTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGGTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATCGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTgg                                              <  2:59728/148‑1 (MQ=255)
                                                                                                        aaGAATGAGTTTAAACCCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATATGCTCCGCCAGATTTTTACCGGTGGc                                             >  1:116386/1‑147 (MQ=255)
                                                                                                        aaGAATGAGTTTAAAACCGCTCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCa                                            <  3:332705/148‑1 (MQ=255)
                                                                                                        aaGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCgg                                                 >  4:48496/1‑143 (MQ=255)
                                                                                                        aaGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCgg                                                 <  3:48496/143‑1 (MQ=255)
                                                                                                          gAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCgg                                                 <  2:279664/141‑1 (MQ=255)
                                                                                                          gAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCgg                                                 >  1:279664/1‑141 (MQ=255)
                                                                                                              gAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATccc                                       >  3:44840/1‑147 (MQ=255)
                                                                                                               aGTTTAAAACCGAGAGCAGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCt                                      <  8:103201/147‑1 (MQ=255)
                                                                                                                 tttAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACAATACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGt                                   <  6:140704/148‑1 (MQ=255)
                                                                                                                     aaaCCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTg                                <  1:983/147‑1 (MQ=255)
                                                                                                                      aaCCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGc                              <  4:272702/148‑1 (MQ=255)
                                                                                                                          gATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCagag                          <  4:220386/148‑1 (MQ=255)
                                                                                                                              gccgcACCAATAAATTCCGCCAGGTCGGTTGCCATCTCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGa                      <  7:317877/148‑1 (MQ=255)
                                                                                                                                cgcACCAATAAATTCCTCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCAAACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGTATc                    <  6:22025/148‑1 (MQ=255)
                                                                                                                                      aaTAAATTCCGCCGGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATc              <  1:349744/148‑1 (MQ=255)
                                                                                                                                      aaTAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGa                      <  3:192323/140‑1 (MQ=255)
                                                                                                                                      aaTAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGa                      >  4:192323/1‑140 (MQ=255)
                                                                                                                                          aaTTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGACCCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGc           <  6:283942/147‑1 (MQ=255)
                                                                                                                                                gCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCa    >  5:9032/1‑148 (MQ=255)
                                                                                                                                                  cAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATc  <  1:81384/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTGAACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATC  >  NC_000913/2512231‑2512524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑