Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,569,859 G→A 6.0% T160M (ACG→ATG)  eutJ ← ethanolamine utilization protein, HSP70/actin superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,569,8590GA 6.0% 212.5 / 2.5 66T160M (ACG→ATG) eutJethanolamine utilization protein, HSP70/actin superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (34/28);  new base A (2/2);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.68e-01

TTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCATCCAGCACATGGCTCACTTCCAGTCCGG  >  NC_000913/2569713‑2569991
                                                                                                                                                  |                                                                                                                                    
ttttttCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGt                                                                                                                                     <  2:17054/148‑1 (MQ=255)
 tttttCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGt                                                                                                                                     >  1:132430/1‑147 (MQ=255)
     tCGTACACCGGTTTCACCGCAGGCCAAATCTCTTTACCGTGACCGCGCTTGTCCTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGGGAGAGTCAGAGAGATGTGCGGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCAcc                                                                                                                                 <  6:294965/147‑1 (MQ=255)
         acacCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGcc                                                                                                                            >  3:134685/1‑148 (MQ=255)
         acacCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGcc                                                                                                                            >  6:140254/1‑148 (MQ=255)
            ccGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCGTGTACTGCTCCGCCGCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGCTGTGATGTCCGCCGGTGGCTTCAGCCGCCGAGTCCGTCACCTTGCCCtg                                                                                                                         >  7:27576/1‑147 (MQ=255)
              ggTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCGTGTACTGCTCCGCCTCTGCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCtttt                                                                                                                       <  2:222277/148‑1 (MQ=255)
                tttCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCttttt                                                                                                                      >  5:85080/1‑147 (MQ=255)
                    aCCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTc                                                                                                                     >  2:330174/1‑144 (MQ=255)
                    aCCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACg                                                                                                                  <  4:134685/147‑1 (MQ=255)
                         aGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCa                                                                                                            <  7:113288/148‑1 (MQ=255)
                         aGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCa                                                                                                            <  6:370300/148‑1 (MQ=255)
                               aaTCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCg                                                                                                      >  5:338998/1‑148 (MQ=255)
                                aTCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCgg                                                                                                     >  2:14888/1‑148 (MQ=255)
                                  ctctTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACATCACCTTGCCCTTTTTCACGATGGCa                                                                                                            <  8:203591/139‑1 (MQ=255)
                                  ctctTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACATCACCTTGCCCTTTTTCACGATGGCa                                                                                                            >  7:203591/1‑139 (MQ=255)
                                     ttCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTa                                                                                                   <  6:313843/145‑1 (MQ=255)
                                       cACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCAAGATGGCAATGCCGGTAGTgc                                                                                               <  1:330174/147‑1 (MQ=255)
                                         ccGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTgccgc                                                                                            >  5:140247/1‑148 (MQ=255)
                                              aCCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTTACGATGGCAATGCCGGTAGTGccgccgccg                                                                                       <  6:85080/148‑1 (MQ=255)
                                              aCCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTgccgccgcc                                                                                        <  3:145061/147‑1 (MQ=255)
                                               ccGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGa                                                                                      <  6:216042/148‑1 (MQ=255)
                                                 gcgcTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGata                                                                                    >  6:358769/1‑148 (MQ=255)
                                                 gcgcTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGata                                                                                    >  4:226489/1‑148 (MQ=255)
                                                      tGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACATCACCTTGCCCttttcacg                                                                                                                   >  6:222045/1‑108 (MQ=255)
                                                      tGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACATCACCTTGCCCttttcacg                                                                                                                   <  5:222045/112‑5 (MQ=255)
                                                        tACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTa                                                                             >  3:356159/1‑148 (MQ=255)
                                                            gCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCt                                                                                                                          >  1:312617/1‑99 (MQ=255)
                                                            gCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCt                                                                                                                          <  2:312617/99‑1 (MQ=255)
                                                            gCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTAcccc                                                                         >  7:196207/1‑148 (MQ=255)
                                                                     ctTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTc                                                                >  6:49034/1‑148 (MQ=255)
                                                                       tCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCTCGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTc                                                                >  5:6472/1‑146 (MQ=255)
                                                                       tCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACt                                                                              >  6:120988/1‑132 (MQ=255)
                                                                       tCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTc                                                                >  3:279867/1‑146 (MQ=255)
                                                                       tCCAGCGAAATACGGCGATTTCCGGCGAGAGGCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACt                                                                              <  5:120988/132‑1 (MQ=255)
                                                                        ccAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTcc                                                               <  4:356159/146‑1 (MQ=255)
                                                                          aGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGtt                                                           >  4:335665/1‑148 (MQ=255)
                                                                              aaaTACGGCGATTTCCGGCGAGAGTCAGAGAGAGGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTgcag                                                       >  1:26909/1‑148 (MQ=255)
                                                                                     gCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATcc                                                >  3:139451/1‑148 (MQ=255)
                                                                                       gATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCATCAGATCCg                                               >  5:335049/1‑147 (MQ=255)
                                                                                         tttCCGGCGAGAGTCAGAGAGATGTGATTTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTa                                                                                                   <  3:173261/93‑1 (MQ=255)
                                                                                         tttCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCAGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTa                                                                                                   >  4:173261/1‑93 (MQ=255)
                                                                                           tCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAAc                                           <  6:155375/147‑1 (MQ=255)
                                                                                             cGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGc                                        <  5:140254/148‑1 (MQ=255)
                                                                                                cGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGt                                     <  5:49034/148‑1 (MQ=255)
                                                                                                       cAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCgg                                                                       >  2:201400/1‑107 (MQ=255)
                                                                                                       cAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCgg                                                                       <  1:201400/107‑1 (MQ=255)
                                                                                                        agagagATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCAt                             <  3:226489/148‑1 (MQ=255)
                                                                                                             gatgtgatgtCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCATCCAg                         >  7:46391/1‑147 (MQ=255)
                                                                                                               tgtgatgtCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCATCCAGcac                      <  2:132430/148‑1 (MQ=255)
                                                                                                                gtgatgtCCGCCGGTGGCTTCCTCCGCCGAGTACGTCACCTTGCCCTTTTTCCCGATGGCAATGCCGGTAGTGCCGCCGACGCTATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCCACTGCGGTTGGCTCATCCAGcaca                     >  6:342454/1‑148 (MQ=255)
                                                                                                                gtgatgtCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATAGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCATCCAGcaca                     >  7:327800/1‑148 (MQ=255)
                                                                                                                 tgatgtCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTCGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCATCCAGCACAt                    <  8:368869/148‑1 (MQ=255)
                                                                                                                   atgtCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCATCCAGCACATg                   <  3:335665/147‑1 (MQ=255)
                                                                                                                    tgtCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCATCCAGcaca                     >  2:267469/1‑144 (MQ=255)
                                                                                                                    tgtCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCATCCAGcaca                     <  1:267469/144‑1 (MQ=255)
                                                                                                                        cgccgGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACt                                                                              >  7:133457/1‑83 (MQ=255)
                                                                                                                        cgccgGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACt                                                                              <  8:133457/83‑1 (MQ=255)
                                                                                                                          ccgGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCATCCAGCACATGGCTCACtt           >  6:32062/1‑148 (MQ=255)
                                                                                                                          ccgGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCATCCAGCACATGGCTCACtt           <  4:139451/148‑1 (MQ=255)
                                                                                                                            ggTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCATCCAGCACATGGCTCACTTcc         <  6:338998/148‑1 (MQ=255)
                                                                                                                            ggTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCATCCAGCACATGGCTCACTTcc         <  1:14888/148‑1 (MQ=255)
                                                                                                                              tGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGCTGGCAATGCCGGTAGTGCCGCCGGCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCCTCCAGCACATGGCTCACTTcccg       >  5:26851/1‑146 (MQ=255)
                                                                                                                                 cTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGTTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCATCCAGCACATGGCTCACTTCCAGTcc    >  5:315094/1‑148 (MQ=255)
                                                                                                                                   tCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCATCCAGCACATGGCTCACTTCCAGTCCgg  >  3:281480/1‑148 (MQ=255)
                                                                                                                                    cATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCATCCAGCACATGGCTCACTTCCAGTCCgg  >  2:8035/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                    
TTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCGCCGATATCCACTACACCGGCGTTGTCCAGTTGCAGCAGATCCGCAACTGCGGTTGGCTCATCCAGCACATGGCTCACTTCCAGTCCGG  >  NC_000913/2569713‑2569991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑