Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,641,450 G→T 9.6% R119S (CGC→AGC)  ispG ← 1‑hydroxy‑2‑methyl‑2‑(E)‑butenyl 4‑diphosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,641,4500GT 9.6% 211.2 / 7.3 68R119S (CGC→AGC) ispG1‑hydroxy‑2‑methyl‑2‑(E)‑butenyl 4‑diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (30/31);  new base T (3/4);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

ATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTGGATGTCAGCCACCAGCGGCACGTTAACCTGCTGTTTGATGAGTTTGAAC  >  NC_000913/2641307‑2641592
                                                                                                                                               |                                                                                                                                              
atCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATATTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGAGCTCACAGTCAACCACCATGCGAATa                                                                                                                                            >  2:153084/1‑148 (MQ=255)
     cATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGctc                                                                                                                                       >  1:291175/1‑148 (MQ=255)
          cGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCAt                                                                                                                                  <  2:190913/148‑1 (MQ=255)
             aTGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTAc                                                                                                                               <  7:127336/148‑1 (MQ=255)
               ggCAGATTCCAGCAACGCCTGCGGCGTCTGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATAGCGCGCACAGTCAACCACCATGCTAATACGCTCTTCATTAcc                                                                                                                              >  7:181565/1‑147 (MQ=255)
               ggCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTAcc                                                                                                                              >  2:53604/1‑147 (MQ=255)
                gCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCg                                                                                                                             <  3:104234/147‑1 (MQ=255)
                gCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGa                                                                                                                            >  2:358633/1‑148 (MQ=255)
                 cAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGat                                                                                                                           <  8:119201/148‑1 (MQ=255)
                 cAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGat                                                                                                                           <  2:108554/148‑1 (MQ=255)
                  aGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGata                                                                                                                          <  3:244877/148‑1 (MQ=255)
                     ttCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTg                                                                                                                       >  2:310562/1‑148 (MQ=255)
                        cAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGcc                                                                                                                     <  8:3455/147‑1 (MQ=255)
                        cAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGcc                                                                                                                     <  4:351085/147‑1 (MQ=255)
                        cAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGc                                                                                                                      >  5:27161/1‑146 (MQ=255)
                         aGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAg                                                                                                                   >  1:90937/1‑148 (MQ=255)
                           cAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAggg                                                                                                                 <  1:53604/148‑1 (MQ=255)
                            aaCGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGt                                                                                                                >  7:201508/1‑148 (MQ=255)
                               gCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTaa                                                                                                             <  8:151113/148‑1 (MQ=255)
                                   gcggcgTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCa                                                                                                                                   <  7:367584/122‑1 (MQ=255)
                                   gcggcgTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCa                                                                                                                                   >  8:367584/1‑122 (MQ=255)
                                       cgTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGa                                                                                                     >  2:122272/1‑148 (MQ=255)
                                                gCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACg                                                                                            <  8:201508/148‑1 (MQ=255)
                                                     aCTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTa                                                                                       >  2:264094/1‑148 (MQ=255)
                                                      cTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTAt                                                                                      >  6:201915/1‑148 (MQ=255)
                                                       ttttCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAAt                                                                                                            >  8:341924/1‑125 (MQ=255)
                                                       ttttCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAAt                                                                                                            <  7:341924/125‑1 (MQ=255)
                                                        tttCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTc                                                                                    >  5:64115/1‑148 (MQ=255)
                                                        tttCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTc                                                                                    >  4:348820/1‑148 (MQ=255)
                                                         ttCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTcc                                                                                   >  1:254369/1‑148 (MQ=255)
                                                               cAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTAc                                                                              <  4:58453/147‑1 (MQ=255)
                                                                 gATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACtt                                                                            >  3:224053/1‑147 (MQ=255)
                                                                 gATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACtt                                                                            <  4:224053/147‑1 (MQ=255)
                                                                      ttttCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCACCCCCCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCCGACAATCGACGCCGTATTCCGCTACTTTCAgcg                                                                      >  6:376958/1‑148 (MQ=255)
                                                                       tttCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCTAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTc                                                                          <  2:18411/143‑1 (MQ=255)
                                                                       tttCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAgcg                                                                      >  7:287870/1‑147 (MQ=255)
                                                                       tttCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAg                                                                        <  1:310562/145‑1 (MQ=255)
                                                                        ttCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCTAATACGCTCTTCATTAcc                                                                                                                              <  3:288442/90‑1 (MQ=255)
                                                                        ttCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCTAATACGCTCTTCATTAcc                                                                                                                              >  4:288442/1‑90 (MQ=255)
                                                                             gCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCg                                                               <  7:335204/148‑1 (MQ=255)
                                                                             gCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCg                                                               <  5:229760/148‑1 (MQ=255)
                                                                             gCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCg                                                               <  5:44797/148‑1 (MQ=255)
                                                                              cgtattggCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGt                                                                                        <  5:19775/116‑1 (MQ=255)
                                                                              cgtattggCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGt                                                                                        >  6:19775/7‑122 (MQ=255)
                                                                               gATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGAt                                                             <  2:90937/148‑1 (MQ=255)
                                                                                 tCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACg                                                                                                         >  5:79604/1‑102 (MQ=255)
                                                                                 tCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACg                                                                                                         <  6:79604/102‑1 (MQ=255)
                                                                                     gCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGGTCTCAGTCAACAACCATTCTAATTCTATCTTCATTAGCGATTTTGCCTGGGTGATTACGCAGACAATCGACGCCGTAGTCCGCTACTTTCAGCGCAATGCGATAGTGGa                                                       <  4:99369/148‑1 (MQ=255)
                                                                                         tAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTg                                                   >  2:46027/1‑148 (MQ=255)
                                                                                             gCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCTAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTGGATg                                               >  5:41336/1‑148 (MQ=255)
                                                                                              ccAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTGGATGt                                              <  1:358633/148‑1 (MQ=255)
                                                                                              ccAATACGGATCGGAATGTTTTTATCGCGCGCACAGGCAACCACCAAGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGCAGTGGATGt                                              <  2:325531/148‑1 (MQ=255)
                                                                                               cAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTGGATGTc                                             <  5:201915/148‑1 (MQ=255)
                                                                                                   aCGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGa                                                       <  1:60332/134‑1 (MQ=255)
                                                                                                   aCGGATAGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGa                                                       >  2:60332/1‑134 (MQ=255)
                                                                                                    cGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCTAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTGGATGTCAGcca                                        <  6:41336/148‑1 (MQ=255)
                                                                                                             aTGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTGGATGTCAGCCACCAGCGGCa                               <  6:27161/148‑1 (MQ=255)
                                                                                                             aTGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTGGATGTCAGCCACCAGCGGCa                               >  6:174445/1‑148 (MQ=255)
                                                                                                               gTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTGGATGTCAGCCACCAGCGGCACg                             >  6:261503/1‑148 (MQ=255)
                                                                                                                  tttATCGCCCGCACAGTCAACCACCATGCGAATACGCTCTTCATTAACGATATTGCCACGCTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGAGCAATGCGAAAGTCGAAGTGGATGTCAGCCACCAGCGTCACgat                           >  1:213261/1‑145 (MQ=255)
                                                                                                                               caGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTGGATGTCAGCCACCAGCGGCACGTTAACCTGCTGTTtga             <  3:348820/148‑1 (MQ=255)
                                                                                                                                 gTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTGGATGTCAGCCACCAGCGGCACGTTAACCTGCTGTTtgat            >  1:121959/1‑147 (MQ=255)
                                                                                                                                   cAACCATCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGCCAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTGGATGTCAGCCACCAGCGGCACGTTAACCTGCTGTTTGATGAg         >  2:107858/1‑148 (MQ=255)
                                                                                                                                    aaCCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTGGATGTCAGCCACCAGCGGCACGTTAACCTGCTGTTTGATGAg         <  6:64115/147‑1 (MQ=255)
                                                                                                                                    aaCCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTGGATGTCAGCCACCAGCGGCACGTTAACCTGCTGTTTGATGAGt        <  1:153084/148‑1 (MQ=255)
                                                                                                                                      ccaccaTGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTGGATGTCAGCCACCAGCGGCACGTTAACCTGCTGTTTGATGAGttt      >  6:91640/1‑148 (MQ=255)
                                                                                                                                        accaTGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTGGATGTCAGCCACCAGCGGCACGTTAACCTGCTGTTTGATGAGTTTGa    <  1:264094/148‑1 (MQ=255)
                                                                                                                                          caTGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTGGATGTCAGCCACCAGCGGCACGTTAACCTGCTGTTTGATGAGTTTGAAc  >  1:384925/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                              
ATCAACATGACGCATGGCAGATTCCAGCAACGCCTGCGGCGTCGGTTCGCCATACTTTTCTTGCAGATCTTTTTCCAGCGATCCGGCGTTAACGCCAATACGGATCGGAATGTTTTTATCGCGCGCACAGTCAACCACCATGCGAATACGCTCTTCATTACCGATATTGCCAGGGTTAATACGCAGACAATCGACGCCGTATTCCGCTACTTTCAGCGCAATGCGATAGTCGAAGTGGATGTCAGCCACCAGCGGCACGTTAACCTGCTGTTTGATGAGTTTGAAC  >  NC_000913/2641307‑2641592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑