Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,896,884 A→T 6.4% C329* (TGT→TGA ygcS ← putative MFS sugar transporter; membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,896,8840AT 6.4% 234.3 / 2.9 76C329* (TGT→TGAygcSputative MFS sugar transporter; membrane protein
Reads supporting (aligned to +/- strand):  ref base A (34/37);  new base T (3/2);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATC  >  NC_000913/2896737‑2897030
                                                                                                                                                   |                                                                                                                                                   
cATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCaaa                                                                                                                                                     >  1:323892/1‑148 (MQ=255)
cATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCaaa                                                                                                                                                     >  7:237943/1‑148 (MQ=255)
  tGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCaaa                                                                                                                                                     >  7:30373/1‑146 (MQ=255)
       gTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCa                                                                                                                                              <  2:84515/148‑1 (MQ=255)
          gCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATg                                                                                                                                            <  8:363990/147‑1 (MQ=255)
          gCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATg                                                                                                                                            <  5:281083/147‑1 (MQ=255)
          gCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATg                                                                                                                                            <  8:261062/147‑1 (MQ=255)
                 cGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAg                                                                                                                                    >  3:207793/1‑148 (MQ=255)
                 cGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAg                                                                                                                                    <  8:298252/148‑1 (MQ=255)
                 cGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAg                                                                                                                                    <  4:128920/148‑1 (MQ=255)
                     gCCCAGCGAGCGAATGTCAGTAGGAACACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGtt                                                                                                                                >  8:314036/1‑148 (MQ=255)
                       ccAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTgc                                                                                                                              >  5:276033/1‑148 (MQ=255)
                        cAGCGAGCGAATGTCAGTAGGATAACTTTCCGCAGGCAAAAAGCCCACCAGATTACTGACTGCCGAAATGGTGTTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATTACTAACAGCGTTg                                                                                                                               >  5:127912/1‑146 (MQ=255)
                         agcgagcgAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTgccg                                                                                                                            >  5:357262/1‑148 (MQ=255)
                         agcgagcgAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTgccg                                                                                                                            >  4:159546/1‑148 (MQ=255)
                           cgagcgaATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTgccgcc                                                                                                                          <  5:207075/148‑1 (MQ=255)
                              gcgaATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCcagc                                                                                                                       <  7:35170/148‑1 (MQ=255)
                                gaATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAATCAGGCCATGACTACCAGCGTTGCCGCcagcag                                                                                                                     >  2:170242/1‑148 (MQ=255)
                                gaATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGGTAATGAACTGCCGGAAGGCAATCAGGCCATGACTACCAGCGTTGCCGCcagcag                                                                                                                     >  2:164977/1‑148 (MQ=255)
                                          aGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACtt                                                                                                          >  1:51567/1‑148 (MQ=255)
                                           ggAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTc                                                                                                         <  8:30373/148‑1 (MQ=255)
                                           ggAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTc                                                                                                         <  1:195024/148‑1 (MQ=255)
                                             aaaaCTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTcc                                                                                                        >  3:184866/1‑147 (MQ=255)
                                                aCTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTTAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAATCAGGCGATGACTACCCGCGTTGCCGCCAGCAGCGAAAACCTTCCCAg                                                                                                     >  6:195837/1‑147 (MQ=255)
                                                   ttCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCaa                                                                                                  >  1:215137/1‑147 (MQ=255)
                                                   ttCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGAGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCACAAAAAACATTCCAAGCaa                                                                                                  >  1:337937/1‑147 (MQ=255)
                                                         aGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTg                                                                                           <  2:370298/148‑1 (MQ=255)
                                                          ggCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGc                                                                                          >  2:308968/1‑148 (MQ=255)
                                                           gCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCg                                                                                         <  8:24304/148‑1 (MQ=255)
                                                           gCAAAATGCCCACCAGATTACTGACTCCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGAAGCGTTAATGAACTGCCGTAAGGCAAACAGGCAATGACTAACAGCGTTGCCGCCCGCAGCAAAACACTGACCCGCACAAATGTGc                                                                                          >  5:118301/1‑147 (MQ=255)
                                                            cAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGa                                                                                        >  4:73445/1‑148 (MQ=255)
                                                            cAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAAAGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGa                                                                                        <  3:9195/148‑1 (MQ=255)
                                                              aaaTGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGAtg                                                                                      >  1:102548/1‑148 (MQ=255)
                                                                 tGCCCACCAGATTACTGACTGCCGAACTGGTGGTGCTGAAGAGAACAAAAAGCATCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCCTGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGc                                                                                   <  6:35565/148‑1 (MQ=255)
                                                                 tGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGc                                                                                   <  4:53526/148‑1 (MQ=255)
                                                                      accaGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCcagca                                                                              <  8:17168/148‑1 (MQ=255)
                                                                          gATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTg                                                                          <  8:259121/148‑1 (MQ=255)
                                                                             tACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGt                                                                       >  3:175534/1‑148 (MQ=255)
                                                                              actgactgCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGt                                                                       >  8:12268/1‑147 (MQ=255)
                                                                              actgactgCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGt                                                                       <  8:237943/147‑1 (MQ=255)
                                                                                  actgCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGaa                                                                  <  7:182383/148‑1 (MQ=255)
                                                                                  actgCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGaa                                                                  >  2:214477/1‑148 (MQ=255)
                                                                                  actgCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTAAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGCGCCAGCAGGTGCGTCAGa                                                                   <  7:244218/147‑1 (MQ=255)
                                                                                  actgCCGAAATGGTCGTGCTGAAGACAACAAAAAGCAGCATCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGaa                                                                  <  5:195837/148‑1 (MQ=255)
                                                                                      ccGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACt                                                                <  3:73445/146‑1 (MQ=255)
                                                                                       cGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAAt                                                             >  5:246025/1‑148 (MQ=255)
                                                                                         aaaTGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGAt                                                                                       <  2:296730/120‑1 (MQ=255)
                                                                                         aaaTGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGAt                                                                                       >  1:296730/1‑120 (MQ=255)
                                                                                          aaTGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATccc                                                          >  5:376272/1‑148 (MQ=255)
                                                                                                     tGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGcc                                               >  5:248111/1‑148 (MQ=255)
                                                                                                      gAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCaaa                                                                                                                                                     <  6:257082/46‑1 (MQ=255)
                                                                                                      gAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCaaa                                                                                                                                                     >  5:257082/1‑46 (MQ=255)
                                                                                                       aaGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGccc                                              <  4:207793/147‑1 (MQ=255)
                                                                                                        agagAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCca                                             >  2:64697/1‑147 (MQ=255)
                                                                                                        acagaACAAAAAGCAGCAGCGATAATGAACTGCCGGAAGGCAAACAGGCCATGACTACAAGCGTTGCCGCCAGCAGCAAAAAACTTCACAGCAAAAATTTGCGATGTGCCAGCAGGTGAGTCAGAACTAATCCAAGCAGCGCGCCca                                             >  8:283443/3‑147 (MQ=255)
                                                                                                          agaACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAACCTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACaa                                          >  3:91633/1‑148 (MQ=255)
                                                                                                          agaACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACaa                                          >  4:212037/1‑148 (MQ=255)
                                                                                                          agaACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGATGCGTCAGAACTAATCCCAGCAGCGCGCCCACaa                                          >  2:376053/1‑148 (MQ=255)
                                                                                                            aaCAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAAtt                                        <  2:215137/148‑1 (MQ=255)
                                                                                                             aCAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTa                                       <  1:108256/148‑1 (MQ=255)
                                                                                                              cAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCcaca                                           >  4:92357/1‑143 (MQ=255)
                                                                                                              cAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCcaca                                           <  3:92357/143‑1 (MQ=255)
                                                                                                               aaaaaGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCCGCAAAAATTTGCGATGTGCCAGCAGGTGCGGCAGAACTAATCCCAGCAGCGCGCCCACAATTaa                                      >  2:235990/1‑147 (MQ=255)
                                                                                                                aaaaGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTaaca                                    <  2:102548/148‑1 (MQ=255)
                                                                                                                  aaGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTaacaac                                  >  2:40712/1‑148 (MQ=255)
                                                                                                                    gcagcagcGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGc                                <  3:212037/148‑1 (MQ=255)
                                                                                                                    gcagcagcGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGc                                <  5:218965/148‑1 (MQ=255)
                                                                                                                        cagcGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACt                                                                                                           <  2:244543/69‑1 (MQ=255)
                                                                                                                        cagcGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACt                                                                                                           >  1:244543/1‑69 (MQ=255)
                                                                                                                          gcGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAg                          >  7:137616/1‑148 (MQ=255)
                                                                                                                            gTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCa                        <  7:12268/148‑1 (MQ=255)
                                                                                                                               aaTGAACTGCCGTAAGGCAAACAGGCCATGACTACCATCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCATCGCGCCCACAATTAACAACGCATTAAGCATc                      <  1:259878/147‑1 (MQ=255)
                                                                                                                               aaTGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATc                      <  4:175534/147‑1 (MQ=255)
                                                                                                                               aaTGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATc                      <  1:308968/147‑1 (MQ=255)
                                                                                                                                    aCTGCCGGAAGGCAATCAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTg                <  1:170242/148‑1 (MQ=255)
                                                                                                                                    aCTGCCGGAAGGCAATCAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTg                <  1:164977/148‑1 (MQ=255)
                                                                                                                                       gCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTgg               >  1:260811/1‑146 (MQ=255)
                                                                                                                                        ccGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGc              <  6:357262/146‑1 (MQ=255)
                                                                                                                                              ggCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAg        >  3:340775/1‑146 (MQ=255)
                                                                                                                                              ggCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAg        <  4:340775/146‑1 (MQ=255)
                                                                                                                                               gCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAgcg      >  7:252388/1‑147 (MQ=255)
                                                                                                                                                 aaaCATGCCATGACTAACAGCGTTGCCGCCAGCAGCAAAAAACTTCCGAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAAGCCCAGCATCGCTCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCAt   <  2:337937/148‑1 (MQ=255)
                                                                                                                                                  aaCAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATc  >  3:342345/1‑148 (MQ=255)
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CATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAACAAAAAGCAGCAGCGTTAATGAACTGCCGGAAGGCAAACAGGCCATGACTACCAGCGTTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATC  >  NC_000913/2896737‑2897030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: