Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,002,530 G→A 100% G729D (GGT→GAT)  xdhA → xanthine dehydrogenase, molybdenum binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,002,5300GA97.1% 224.5 / ‑2.6 69G729D (GGT→GAT) xdhAxanthine dehydrogenase, molybdenum binding subunit
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (26/40);  total (27/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGACCATGCCGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGGTGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAACCTGCTGG  >  NC_000913/3002386‑3002674
                                                                                                                                                |                                                                                                                                                
cGACCATGCCGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAATTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGatgt                                                                                                                                               <  2:231736/148‑1 (MQ=255)
 gACCATGCAGTATCTGCCACACCTGGAAATCGCTTGCGTCGAAAGCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGAGTTGAAGATGGCTACCGATGtt                                                                                                                                              <  6:288850/148‑1 (MQ=255)
  aCCATGCCGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTg                                                                                                                                             >  1:52458/1‑148 (MQ=255)
  aCCATGCCGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTg                                                                                                                                             <  2:229905/148‑1 (MQ=255)
   ccATGCCGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAACTCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGGGAAGATGGCTACCGATGTTGc                                                                                                                                            <  4:243717/148‑1 (MQ=255)
    cATGCCGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGca                                                                                                                                           <  6:222610/148‑1 (MQ=255)
        ccGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGcaatc                                                                                                                                        >  2:363682/1‑147 (MQ=255)
          ggATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGcaatcaat                                                                                                                                     <  7:220993/148‑1 (MQ=255)
          ggATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGcaatcaa                                                                                                                                      >  2:70075/1‑147 (MQ=255)
                  cacaACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATGCGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGcc                                                                                                                             >  5:53686/1‑148 (MQ=255)
                      aCTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCCTACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGACGCTGCGATTCGTAACGCGGTGAAGCTGGCTACCGATGTTGCAATCAAGACACTGCCGCTg                                                                                                                         >  8:332052/1‑148 (MQ=255)
                      aCTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTg                                                                                                                         <  7:349399/148‑1 (MQ=255)
                               cgcgTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCaaaa                                                                                                                >  4:15826/1‑148 (MQ=255)
                                   ttCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGt                                                                                                            <  7:151298/148‑1 (MQ=255)
                                     cgtcgAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGtt                                                                                                           <  2:52458/147‑1 (MQ=255)
                                      gtcgAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTtat                                                                                                         <  1:314087/148‑1 (MQ=255)
                                          aaaTCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGCGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATg                                                                                                      >  4:335566/1‑147 (MQ=255)
                                            aTCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATg                                                                                                      >  7:373444/1‑145 (MQ=255)
                                            aTCAATGAGCCGCAATCCGAATACGGACATAAGTCACTGTGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCTATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAag                                                                                                   <  5:54833/148‑1 (MQ=255)
                                              cAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAagag                                                                                                 >  8:26060/1‑148 (MQ=255)
                                                    gCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGGTGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCAt                                                                                           <  3:98006/148‑1 (MQ=255)
                                                     ccGCAATCCGCATACTGACATTAGTGACTGGTTGATCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACTCGGTGAAGATGGCTACCAATGTTTCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATc                                                                                          <  2:184462/148‑1 (MQ=255)
                                                         aaTCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTgg                                                                                       >  1:375105/1‑147 (MQ=255)
                                                             cGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGga                                                                                  >  1:318056/1‑148 (MQ=255)
                                                              gCATCCGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGgat                                                                                 <  6:53686/148‑1 (MQ=255)
                                                                 tACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGgattga                                                                              >  5:45103/1‑148 (MQ=255)
                                                                  aCGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGgattgat                                                                             >  4:176308/1‑148 (MQ=255)
                                                                     gACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTg                                                                          <  8:373444/148‑1 (MQ=255)
                                                                     gACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTg                                                                          <  2:375105/148‑1 (MQ=255)
                                                                     gACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGCGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTg                                                                          <  3:335566/148‑1 (MQ=255)
                                                                        aTAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAAGCACTACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGCTCCATCTGGCAGGATTGATTTGAgg                                                                       <  2:273612/148‑1 (MQ=255)
                                                                           aGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTGGTAACGCGGGGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATa                                                                    <  5:53734/148‑1 (MQ=255)
                                                                             tCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATaa                                                                   >  6:208128/1‑147 (MQ=255)
                                                                                cTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAAcatc                                                               <  4:225600/148‑1 (MQ=255)
                                                                                  gggTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAAcatcat                                                             >  6:13134/1‑148 (MQ=255)
                                                                                    gTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTGGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGt                                                           >  2:245167/1‑148 (MQ=255)
                                                                                       aGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTg                                                        <  6:45103/148‑1 (MQ=255)
                                                                                        gCCACCAATAATTCCTGCTGCCGCTGCTATTCGTAACGCGGTTAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGa                                                       <  3:97683/148‑1 (MQ=255)
                                                                                         ccaccaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGGTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGAt                                                      <  2:354784/148‑1 (MQ=255)
                                                                                          caccaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGAtt                                                     >  3:296459/1‑148 (MQ=255)
                                                                                           accaTTAATTCCTGTTGCCGCTGCTATTCGTATCGCGGGGAATTTGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGGCAATTCGGTTTTATGAAGAGTTCCATCTGGCAGGATTGATTTGAGTATAAcat                                                                <  8:179148/136‑1 (MQ=255)
                                                                                             caATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTg                                                  >  5:115772/1‑148 (MQ=255)
                                                                                             caATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTg                                                  >  2:208316/1‑148 (MQ=255)
                                                                                                taatTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGctt                                               <  2:318056/148‑1 (MQ=255)
                                                                                                   ttCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGGCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGcttct                                             >  6:656/1‑147 (MQ=255)
                                                                                                   ttCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGcttct                                             >  2:13346/1‑147 (MQ=255)
                                                                                                       tGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCa                                        >  2:45659/1‑148 (MQ=255)
                                                                                                        gTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTg                                                                          <  5:189314/113‑1 (MQ=255)
                                                                                                        gTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTg                                                                          >  6:189314/1‑113 (MQ=255)
                                                                                                             cGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCgcgc                                  <  1:45659/148‑1 (MQ=255)
                                                                                                             cGCTGCTATTCGAAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGATCCCTCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCgcgc                                  <  1:122452/148‑1 (MQ=255)
                                                                                                                  ctATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGGCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCgcagc                               <  5:656/146‑1 (MQ=255)
                                                                                                                  ctATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCgcagca                              <  1:363682/147‑1 (MQ=255)
                                                                                                                  ctATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCgcagc                               <  2:160982/146‑1 (MQ=255)
                                                                                                                     ttCGTAACGCGGTGAAGATGGCTACCGGTGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAAccc                          >  5:135650/1‑148 (MQ=255)
                                                                                                                          aaCGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGcc                     >  4:87157/1‑148 (MQ=255)
                                                                                                                           aCGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCg                    <  6:115772/148‑1 (MQ=255)
                                                                                                                           aCGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCg                    <  1:360340/148‑1 (MQ=255)
                                                                                                                           aCGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCg                    <  3:15826/148‑1 (MQ=255)
                                                                                                                           aCGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCg                    <  3:176308/148‑1 (MQ=255)
                                                                                                                              cgGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATg                 <  4:296459/148‑1 (MQ=255)
                                                                                                                                gTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGcc               <  7:26060/148‑1 (MQ=255)
                                                                                                                                gTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGcc               <  3:87157/148‑1 (MQ=255)
                                                                                                                                  gAAGATGGCGACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCAt             <  6:370387/148‑1 (MQ=255)
                                                                                                                                     gATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCaa          <  5:13134/148‑1 (MQ=255)
                                                                                                                                          cTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAACctgc     <  1:13346/148‑1 (MQ=255)
                                                                                                                                            aCCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAACctgctg   >  2:72975/1‑148 (MQ=255)
                                                                                                                                             ccGCTGTTGCAATCAAGACCCTGCCGCTGCCGCCAAAACGGTTATATGAAGCGTTCCATCTGGCAGGATTGATTTGAGGTTAACATCATGTTGGATTTTGCTGCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAACCTgctgg  <  6:113717/148‑1 (MQ=255)
                                                                                                                                             ccGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCACCCCTTGCCGATGCCATCAACCTgctgg  >  8:171319/1‑148 (MQ=255)
                                                                                                                                             ccGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAACctgctg   <  1:208316/147‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CGACCATGCCGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGGTGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAACCTGCTGG  >  NC_000913/3002386‑3002674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: