Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,075,097 C→T 5.4% G33G (GGG→GGA yggD ← MtlR family putative transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,075,0970CT 5.4% 243.0 / 2.4 74G33G (GGG→GGAyggDMtlR family putative transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (36/34);  new base T (2/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-01

TTTATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACA  >  NC_000913/3074950‑3075243
                                                                                                                                                   |                                                                                                                                                  
tttATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATc                                                                                                                                                    >  2:88694/1‑148 (MQ=255)
tttATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGAGGGCCGCTTTGTGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTAACGACAACCAGCGACGGCAGAAACTGGCGTATc                                                                                                                                                    >  6:131485/1‑148 (MQ=255)
  tATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATcc                                                                                                                                                   <  7:5923/147‑1 (MQ=255)
    tCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCt                                                                                                                                                <  1:88694/148‑1 (MQ=255)
     ccATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTg                                                                                                                                               >  6:172658/1‑148 (MQ=255)
      cATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGTCGTATCCCCTg                                                                                                                                               >  2:226820/1‑147 (MQ=255)
         tttCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAg                                                                                                                                            >  2:372767/1‑147 (MQ=255)
            ccccAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCaa                                                                                                                                         >  2:5618/1‑147 (MQ=255)
             cccAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCaaa                                                                                                                                        <  2:165251/147‑1 (MQ=255)
              ccAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCGTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAAt                                                                                                                                      >  7:201771/1‑148 (MQ=255)
                 aCGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGt                                                                                                                                   <  5:250545/148‑1 (MQ=255)
                 aCGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGt                                                                                                                                   <  7:348546/148‑1 (MQ=255)
                 aCGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGt                                                                                                                                   <  8:86950/148‑1 (MQ=255)
                       aaaTCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATgcg                                                                                                                              >  5:72675/1‑147 (MQ=255)
                         aTCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGAATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAg                                                                                                                           >  2:311339/1‑148 (MQ=255)
                           cAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGtt                                                                                                                         >  1:323191/1‑148 (MQ=255)
                            aaaCGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGtt                                                                                                                         <  8:141792/147‑1 (MQ=255)
                                  aGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGATTTTCGGGTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATaa                                                                                                                  <  1:179953/148‑1 (MQ=255)
                                   gTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGTCAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATaaa                                                                                                                 <  5:131485/148‑1 (MQ=255)
                                   gTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATaaa                                                                                                                 <  5:53513/148‑1 (MQ=255)
                                           tCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGCCGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAaaat                                                                                                          >  8:38932/1‑147 (MQ=255)
                                           tCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTAATAAATGAaaat                                                                                                          >  2:204507/1‑147 (MQ=255)
                                            cAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAaaata                                                                                                         >  3:346952/1‑147 (MQ=255)
                                             aaTATCGTCAAGCTGGCAGCTTTTGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATAGTTATCGACAAACATCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTCTGGGCAGTTTTCATAAATGAaaataa                                                                                                        <  1:204507/147‑1 (MQ=255)
                                             aaTATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAaaataaa                                                                                                       >  7:119714/1‑148 (MQ=255)
                                                     cAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATTCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCtg                                                                                               >  5:52664/1‑148 (MQ=255)
                                                     cAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATTCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCtg                                                                                               >  5:57961/1‑148 (MQ=255)
                                                        gCGGGCCGCGTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACCGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGc                                                                                            <  6:308930/148‑1 (MQ=255)
                                                         cGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGATATGCGCAGTTTTCATAAAt                                                                                                                <  5:56480/127‑1 (MQ=255)
                                                         cGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGATATGCGCAGTTTTCATAAAt                                                                                                                >  6:56480/1‑127 (MQ=255)
                                                          gggCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCAt                                                                                          >  7:169818/1‑148 (MQ=255)
                                                            gCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATcc                                                                                        <  4:346194/148‑1 (MQ=255)
                                                               gCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGt                                                                                     >  7:232288/1‑148 (MQ=255)
                                                                  ttGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCGGTGCATCCAGTTGc                                                                                  >  1:151808/1‑148 (MQ=255)
                                                                   tGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATTCCCTGGAGCAAAATGGTATGCTCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTg                                                                                   <  6:52664/146‑1 (MQ=255)
                                                                   tGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATTCCCTGGAGCAAAATGGTATGCCCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTg                                                                                   <  6:57961/146‑1 (MQ=255)
                                                                        gAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGc                                                                            <  8:201771/148‑1 (MQ=255)
                                                                           tAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGcaca                                                                         >  7:112601/1‑148 (MQ=255)
                                                                              cGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTATGCATCCAGTTGCTCAAGCAcatca                                                                      <  3:13427/148‑1 (MQ=255)
                                                                                 cTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCAtct                                                                   >  8:80302/1‑148 (MQ=255)
                                                                                 cTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGAATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCAtct                                                                   <  3:44550/148‑1 (MQ=255)
                                                                                  ttCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGAAGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCAtctt                                                                  >  4:223520/1‑148 (MQ=255)
                                                                                    cACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCAtcttct                                                                >  6:198649/1‑148 (MQ=255)
                                                                                       tGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGt                                                              >  3:181733/1‑147 (MQ=255)
                                                                                          atatTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGc                                                          >  1:298787/1‑148 (MQ=255)
                                                                                            atTACACGATCTCTTCATCGTTATCGACAAACAGCGACGCCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATAGTCATGTGCATCCAGTTGATCAAGCACATCAACTTCTGTCAGCg                                                         >  3:336315/1‑147 (MQ=255)
                                                                                             ttCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGt                                                        <  8:232288/147‑1 (MQ=255)
                                                                                              tCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCg                                                      >  6:153581/1‑148 (MQ=255)
                                                                                               ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGc                                                     >  2:352150/1‑148 (MQ=255)
                                                                                                cACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGt                                                                                                   <  7:371752/101‑1 (MQ=255)
                                                                                                cACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGt                                                                                                   >  2:202880/1‑101 (MQ=255)
                                                                                                cACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGt                                                                                                   >  8:371752/1‑101 (MQ=255)
                                                                                                cACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGt                                                                                                   <  1:202880/101‑1 (MQ=255)
                                                                                                cACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCt                                                                                                <  5:134490/104‑1 (MQ=255)
                                                                                                cACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCt                                                                                                >  6:134490/1‑104 (MQ=255)
                                                                                                cACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGcc                                                    <  5:198649/148‑1 (MQ=255)
                                                                                                    aTCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTg                                                >  8:34531/1‑148 (MQ=255)
                                                                                                           cATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTc                                          <  6:72675/147‑1 (MQ=255)
                                                                                                            aTCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTcc                                         >  5:296359/1‑147 (MQ=255)
                                                                                                              cGTTATCGACAAACCGCGACGGCAGAAACTGGCGTATCCCCTCGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATAGTCCTCTGCATCCAGTTGCTCAAGCCCAGCAGCTTCTCTCACCGTCGCCAATGGGCTTCCgc                                       >  4:252169/1‑146 (MQ=255)
                                                                                                                 tATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCAcat                                                                        <  5:142152/111‑1 (MQ=255)
                                                                                                                 tATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCAcat                                                                        >  6:142152/1‑111 (MQ=255)
                                                                                                                   tCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATc                                 <  8:119714/148‑1 (MQ=255)
                                                                                                                      aCAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCAt                                                                                          >  1:114697/1‑88 (MQ=255)
                                                                                                                      aCAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCAt                                                                                          <  2:114697/88‑1 (MQ=255)
                                                                                                                          aCAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCa                          <  5:153581/148‑1 (MQ=255)
                                                                                                                           cAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCaa                         <  1:311339/148‑1 (MQ=255)
                                                                                                                               gACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGGCTTCCGATAGATCACCATCAATGCa                     <  6:376591/148‑1 (MQ=255)
                                                                                                                                  ggCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAggg                  <  4:346952/148‑1 (MQ=255)
                                                                                                                                  ggCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAggg                  <  3:223520/148‑1 (MQ=255)
                                                                                                                                    cAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCTTCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAg                <  7:38932/148‑1 (MQ=255)
                                                                                                                                    cAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAg                >  8:84461/1‑148 (MQ=255)
                                                                                                                                      gAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTc              >  3:321477/1‑148 (MQ=255)
                                                                                                                                              cGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCtattatat      >  7:204289/1‑148 (MQ=255)
                                                                                                                                              cGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCtattatat      <  2:323191/148‑1 (MQ=255)
                                                                                                                                              cGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGGGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCtattatat      <  5:258402/148‑1 (MQ=255)
                                                                                                                                                  tCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTaca  <  6:296359/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACA  >  NC_000913/3074950‑3075243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: