| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,223,594 | A→T | 5.1% | K321I (AAA→ATA) | ebgA → | evolved beta‑D‑galactosidase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,223,594 | 0 | A | T | 5.1% | 246.2 / 2.9 | 79 | K321I (AAA→ATA) | ebgA | evolved beta‑D‑galactosidase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (39/36); new base T (2/2); total (41/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
AGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATA > NC_000913/3223449‑3223737 | cgccccAGCTTTGCTATTCCTGCCGAACAGCCGCACCAATGGTCAGCAGAATCCCCATCTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAg < 2:323573/144‑1 (MQ=255)aGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAg > 7:84598/1‑148 (MQ=255) ctttgctttTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGAc < 8:279029/148‑1 (MQ=255) ttgctttTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACgg > 7:344886/1‑148 (MQ=255) ttgctttTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGAAGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACACCGCGTTGGCTTCCGTGAGATCAAAGTGCGCGACgg > 1:322097/1‑148 (MQ=255) tgctttTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACgg < 8:344886/147‑1 (MQ=255) tttACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGt > 6:343820/1‑148 (MQ=255) gTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGa > 8:378352/1‑148 (MQ=255) tCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGa < 8:84598/147‑1 (MQ=255) gCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTc > 4:242898/1‑136 (MQ=255) gCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTc < 3:242898/136‑1 (MQ=255) agcaATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTa > 8:12640/1‑148 (MQ=255) agcaATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTa < 5:110344/148‑1 (MQ=255) ggTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGAt > 7:352744/1‑148 (MQ=255) gTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGAt < 1:171912/147‑1 (MQ=255) cagcagAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGAtgct > 5:225393/1‑148 (MQ=255) agcagAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGAtgctg < 6:146400/148‑1 (MQ=255) gcagAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGAtgctgc < 7:24979/148‑1 (MQ=255) cagAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCa > 1:172269/1‑148 (MQ=255) aaTCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACTGTCTGTTCTGGATCAATAACCGTTATTTAATGCTGCACgg > 2:26251/1‑148 (MQ=255) ccTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTc > 2:165934/1‑147 (MQ=255) cTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCaa > 5:168013/1‑148 (MQ=255) tATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAAcc > 5:358944/1‑148 (MQ=255) tATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAAcc > 5:372266/1‑148 (MQ=255) aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTAtgtg < 5:212684/128‑1 (MQ=255) aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTAtgtg > 6:212684/1‑128 (MQ=255) tttACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCa > 1:196973/1‑148 (MQ=255) ccATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTAtg < 3:275866/119‑1 (MQ=255) ccATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTAtg > 4:275866/1‑119 (MQ=255) aTCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTTATATCATAGTGCGAGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCCACCGTCACGAcca > 1:312538/1‑146 (MQ=255) aTCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCATAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACaa > 1:317598/1‑148 (MQ=255) ggTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGat > 3:13596/1‑148 (MQ=255) cATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACATCGatcat > 8:131915/1‑148 (MQ=255) tGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCg > 8:145673/1‑148 (MQ=255) gACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCg > 2:160497/1‑147 (MQ=255) gACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCg > 2:236427/1‑147 (MQ=255) gACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCg > 8:38717/1‑147 (MQ=255) aCGCTGAAAGACGCCCACGGCAACGTTCTGGAAGTGGTTCCACAACGCGTTGGCGTCCGTTATTTCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCa < 5:259361/148‑1 (MQ=255) cGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCaa < 3:324608/148‑1 (MQ=255) cGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCaa < 2:172269/148‑1 (MQ=255) gAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGc > 2:186057/1‑148 (MQ=255) aaGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCcg > 2:35567/1‑148 (MQ=255) gACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGCCAACGATCATCGCAAAGGCcgcg > 2:24010/1‑148 (MQ=255) gCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCTCAAAGGCCGCGCCg > 8:196438/1‑148 (MQ=255) ccAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGCTATCATAGTGCGCGACGGTCTGGTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGt < 2:317598/148‑1 (MQ=255) ccAACGGCAACGGTCTGGAAGCGGTGCCACCACGCGTTGGCTTCCGTGATATCATAGTGCGCGACGGGCTGGGCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGAGCATCGCAAAGGCCGCGCCGt < 2:312538/148‑1 (MQ=255) cAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTc > 2:253821/1‑116 (MQ=255) cAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTc < 1:253821/116‑1 (MQ=255) aaCGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTg > 8:362480/1‑148 (MQ=255) cGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGa < 7:38717/148‑1 (MQ=255) aCGTTCTGGAAGTGTTGCCACAACGCGTTGGCTTCCGTGATATCTAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGTCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGa < 3:138314/148‑1 (MQ=255) aCGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGa < 5:343820/148‑1 (MQ=255) aCGGTCTGGAAGGGGTGCCACAACGCGTTGGCGTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGa < 2:310736/148‑1 (MQ=255) ttCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATcg < 4:13596/148‑1 (MQ=255) ggAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTAGGAATGGATCGCGTc > 4:235222/1‑148 (MQ=255) tggtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGc > 2:329263/1‑86 (MQ=255) tggtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGc < 1:329263/86‑1 (MQ=255) tggtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGaa < 7:196438/148‑1 (MQ=255) tggtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGaa < 7:249421/148‑1 (MQ=255) tgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACTACAACGATCATCGCAAAGGCCGCGACGTTGGAATGGATCGCGTCgaga > 1:289376/1‑144 (MQ=255) tgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGa < 1:35567/148‑1 (MQ=255) cacaACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCCACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtct > 6:144574/1‑148 (MQ=255) cacaACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtct < 6:372266/148‑1 (MQ=255) acaACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGGCTGCTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtctc < 7:378352/148‑1 (MQ=255) caACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTcc < 6:225393/148‑1 (MQ=255) caACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTcc < 2:196973/148‑1 (MQ=255) aCGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAg > 8:84538/1‑148 (MQ=255) aCGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAg > 4:308149/1‑148 (MQ=255) aCGCGTTGGCTTCCGTGATATCAAAGTGAGCGACGGTCTGTTCTGGATCAATAACCGTTTTGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAg < 6:358944/148‑1 (MQ=255) cgTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtg < 6:168013/148‑1 (MQ=255) tCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGaa > 1:385740/1‑127 (MQ=255) tCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGaa < 2:385740/127‑1 (MQ=255) atatCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGcaca < 3:308149/147‑1 (MQ=255) atatCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGcaca < 8:352744/147‑1 (MQ=255) atatCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACaa < 7:362480/148‑1 (MQ=255) atatCAAAGTGCGCGACGGTCTGTTCTGGATAAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGACGTTGGAATGGATCGAGTCGAGAAAGATCTCCAGTTGATTAAGAAGcaca > 6:216644/1‑147 (MQ=255) tatCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAat < 1:236427/148‑1 (MQ=255) tatCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGAGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACaa > 2:254183/1‑147 (MQ=255) tCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAata < 1:186057/147‑1 (MQ=255) | AGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATA > NC_000913/3223449‑3223737 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |