Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,439,728 G→T 5.0% A91D (GCC→GAC)  rplQ ← 50S ribosomal subunit protein L17

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,439,7280GT 5.0% 362.2 / 2.6 103A91D (GCC→GAC) rplQ50S ribosomal subunit protein L17
Reads supporting (aligned to +/- strand):  ref base G (54/43);  new base T (3/3);  total (57/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

ACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAG  >  NC_000913/3439581‑3439870
                                                                                                                                                   |                                                                                                                                              
aCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGCAAGCCACACTTCAGAATACGAGTGTAACCACCgg                                                                                                                                                >  6:230885/1‑148 (MQ=255)
     ccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGTCACgg                                                                                                                                           >  7:209056/1‑148 (MQ=255)
     ccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGTCACgg                                                                                                                                           >  7:214422/1‑148 (MQ=255)
           cGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTcgcg                                                                                                                                     <  2:139792/148‑1 (MQ=255)
               tttttttACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAAc                                                                                                                                 >  3:3953/1‑148 (MQ=255)
               tttttttACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAAc                                                                                                                                 >  7:340021/1‑148 (MQ=255)
                ttttttACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGaa                                                                                                                                   <  3:166589/145‑1 (MQ=255)
                ttttttACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGaa                                                                                                                                   >  4:166589/1‑145 (MQ=255)
                  ttttACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAAcgcg                                                                                                                              >  3:211449/1‑148 (MQ=255)
                   tttACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAAcgcg                                                                                                                              <  1:62976/147‑1 (MQ=255)
                   tttACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAAcgcg                                                                                                                              <  5:123390/147‑1 (MQ=255)
                   tttACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCgg                                                                                                                             >  1:271845/1‑148 (MQ=255)
                    ttACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCggg                                                                                                                            >  3:47780/1‑148 (MQ=255)
                           gCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGt                                                                                                                      <  1:25627/147‑1 (MQ=255)
                            cTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGtt                                                                                                                     <  8:35157/147‑1 (MQ=255)
                              tCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGt                                                                                                                  >  4:125211/1‑148 (MQ=255)
                              tCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGt                                                                                                                  >  2:88127/1‑148 (MQ=255)
                               cAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCg                                                                                                                   <  8:176633/146‑1 (MQ=255)
                                   ttACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTaaa                                                                                                              >  5:67556/1‑147 (MQ=255)
                                      ctctGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGt                                                                                                          <  4:180595/148‑1 (MQ=255)
                                        ctGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGttt                                                                                                        >  1:43061/1‑148 (MQ=255)
                                        ctGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGttt                                                                                                        >  6:163324/1‑148 (MQ=255)
                                         tGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGtt                                                                                                         <  6:174192/146‑1 (MQ=255)
                                         tGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGtt                                                                                                         <  2:74014/146‑1 (MQ=255)
                                         tGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGtt                                                                                                         <  8:340021/146‑1 (MQ=255)
                                           cagcagCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGtttt                                                                                                       <  2:43061/146‑1 (MQ=255)
                                           cagcagCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGGCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGttt                                                                                                        <  1:128280/145‑1 (MQ=255)
                                             gcagcTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCa                                                                                                   <  8:316241/148‑1 (MQ=255)
                                               agcTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCAc                                                                                                  >  1:155462/1‑147 (MQ=255)
                                                gcTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGa                                                                                                <  5:195813/148‑1 (MQ=255)
                                                    cTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGAtct                                                                                             <  7:315840/147‑1 (MQ=255)
                                                      gCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGt                                                                                          >  5:244957/1‑148 (MQ=255)
                                                       cTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCg                                                                                           <  6:100593/146‑1 (MQ=255)
                                                       cTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGtt                                                                                         <  8:6425/148‑1 (MQ=255)
                                                         ttCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTa                                                                                        >  4:327777/1‑147 (MQ=255)
                                                         ttCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGACACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTAGTTAAACAGTTTTGCCACGATCTCGTTa                                                                                        >  3:349701/1‑147 (MQ=255)
                                                           ctctGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATc                                                                                      <  2:271845/147‑1 (MQ=255)
                                                               gAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGt                                                                                                                      <  4:330237/111‑1 (MQ=255)
                                                               gAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGt                                                                                                                      >  3:330237/1‑111 (MQ=255)
                                                               gAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCa                                                                                     <  6:57297/144‑1 (MQ=255)
                                                               gAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCa                                                                                     >  5:57297/1‑144 (MQ=255)
                                                               gAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAg                                                                                 >  5:328595/1‑148 (MQ=255)
                                                                aaCGATCACCCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGt                                                                                <  3:24560/148‑1 (MQ=255)
                                                                aaCGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACg                                                                                   <  3:168650/145‑1 (MQ=255)
                                                                aaCGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACg                                                                                   <  4:211449/145‑1 (MQ=255)
                                                                aaCGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACg                                                                                   <  1:261107/145‑1 (MQ=255)
                                                                aaCGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGt                                                                                <  6:234032/148‑1 (MQ=255)
                                                                    aTCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACCCTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAACCGCGGGCCCAGTTCGTTAAACAGTTTTGCCCCGATCTCGTTATCACGAGTACgg                                                                            >  6:212607/1‑148 (MQ=255)
                                                                    aTCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATAC‑‑GTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGc                                                                          >  5:25360/1‑148 (MQ=255)
                                                                           aGCTCGATGTAAGCCATCGGCGCGTTTTCGACTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATg                                                                     <  5:230885/148‑1 (MQ=255)
                                                                            gCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGc                                                                    >  8:219179/1‑148 (MQ=255)
                                                                             cTCGCTGTTAGCCAGCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGcc                                                                   <  3:73440/148‑1 (MQ=255)
                                                                             cTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCGCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCGCGAGTACGGGCGAATGcc                                                                   <  5:272307/148‑1 (MQ=255)
                                                                              tCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGc                                                                    >  1:161489/1‑146 (MQ=255)
                                                                                    tAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAgacgac                                                            >  6:309726/1‑148 (MQ=255)
                                                                                      aGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCGCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCCCGATCTCGTTATCACGAGTACGGGCGAATGCCAGacgacg                                                           <  4:342088/147‑1 (MQ=255)
                                                                                       gCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGAt                                                         >  2:2687/1‑148 (MQ=255)
                                                                                            cGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGtt                                                                                                         <  4:247539/95‑1 (MQ=255)
                                                                                            cGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGtt                                                                                                         >  3:247539/1‑95 (MQ=255)
                                                                                            cGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGc                                                     >  2:238516/1‑147 (MQ=255)
                                                                                            cGGCGCGTTGTCGCCTGCACGGAAGCAACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGc                                                     >  1:379956/1‑147 (MQ=255)
                                                                                             ggCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGTCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCa                                                    <  8:214422/147‑1 (MQ=255)
                                                                                             ggCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGTCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCa                                                    <  8:209056/147‑1 (MQ=255)
                                                                                               cgcgTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGCATGCCAGACGACGATTAGCACCg                                                 >  4:112470/1‑148 (MQ=255)
                                                                                                       cGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACg                                                                                   >  7:221570/1‑106 (MQ=255)
                                                                                                       cGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACg                                                                                   <  8:221570/106‑1 (MQ=255)
                                                                                                            gCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTgg                                    >  4:117231/1‑148 (MQ=255)
                                                                                                              aCGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGc                                   >  3:165060/1‑147 (MQ=255)
                                                                                                                  aaGCCACCCTTCAGAATACGAGTGTAACCACCGGCCCGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAagag                              <  8:37606/148‑1 (MQ=255)
                                                                                                                   aGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGt                             <  8:162841/148‑1 (MQ=255)
                                                                                                                   aGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGt                             <  1:88127/148‑1 (MQ=255)
                                                                                                                     ccACACTTCAGAATACGAGTGTAACCACCGTCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTaa                           >  4:205715/1‑148 (MQ=255)
                                                                                                                     ccACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTaa                           <  5:309726/148‑1 (MQ=255)
                                                                                                                      cacaCTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAAt                          >  3:50327/1‑148 (MQ=255)
                                                                                                                      cacaCTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAAt                          >  1:45641/1‑148 (MQ=255)
                                                                                                                        cacTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAAt                          >  7:372691/1‑146 (MQ=255)
                                                                                                                         acTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAg                       >  4:136102/1‑148 (MQ=255)
                                                                                                                          cTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGc                      >  8:103828/1‑148 (MQ=255)
                                                                                                                           ttCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCAACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCg                     >  2:258264/1‑148 (MQ=255)
                                                                                                                            tCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCgg                    >  4:324383/1‑148 (MQ=255)
                                                                                                                            tCAGAATACGACTGTAACCACCGGCACGGCTCCCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGAACTCGTTATCACAAGTACGCGCGAAAGCCAGACGACGAATAGCACCGCTATCAGTCTTGGCAACAGTAATCAGCgg                    >  2:338283/1‑148 (MQ=255)
                                                                                                                                aaTACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTc                 >  3:357634/1‑147 (MQ=255)
                                                                                                                                aaTACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCa                <  3:205715/148‑1 (MQ=255)
                                                                                                                                   aCGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAAc              >  2:148509/1‑147 (MQ=255)
                                                                                                                                   aCGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAAc              >  5:230754/1‑147 (MQ=255)
                                                                                                                                     gAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTAc           >  4:181231/1‑148 (MQ=255)
                                                                                                                                      aGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTATGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAagag                              >  6:285753/1‑128 (MQ=255)
                                                                                                                                      aGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTATGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAagag                              <  5:285753/128‑1 (MQ=255)
                                                                                                                                       gtgtAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTAcgc         >  8:250391/1‑148 (MQ=255)
                                                                                                                                        tgtAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTAcgcg        >  1:372604/1‑148 (MQ=255)
                                                                                                                                         gtAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCt                                       >  3:314918/1‑116 (MQ=255)
                                                                                                                                         gtAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACgcgg       >  7:314619/1‑148 (MQ=255)
                                                                                                                                           aaCCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGc                      >  8:217081/1‑131 (MQ=255)
                                                                                                                                           aaCCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGc                      <  7:217081/131‑1 (MQ=255)
                                                                                                                                            accaccGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGgcgc    <  2:161489/148‑1 (MQ=255)
                                                                                                                                              caccGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAg  >  8:55397/1‑148 (MQ=255)
                                                                                                                                               accGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGc                   >  2:252261/1‑130 (MQ=255)
                                                                                                                                               accGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGc                   <  1:252261/130‑1 (MQ=255)
                                                                                                                                               accGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACgcggcg     <  8:273453/144‑1 (MQ=255)
                                                                                                                                               accGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACgcggcg     >  7:273453/1‑144 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
ACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAG  >  NC_000913/3439581‑3439870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: