Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,806,430 C→A 5.6% R213L (CGC→CTC)  waaG ← UDP‑glucose:(heptosyl)lipopolysaccharide alpha‑1,3‑glucosyltransferase; lipopolysaccharide core biosynthesis protein; lipopolysaccharide glucosyltransferase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,806,4300CA 5.6% 444.8 / 5.5 140R213L (CGC→CTC) waaGUDP‑glucose:(heptosyl)lipopolysaccharide alpha‑1,3‑glucosyltransferase; lipopolysaccharide core biosynthesis protein; lipopolysaccharide glucosyltransferase I
Reads supporting (aligned to +/- strand):  ref base C (84/47);  new base A (2/7);  total (86/54)
Fisher's exact test for biased strand distribution p-value = 2.74e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

TGCGACCGGAGAAGAAATGCACATTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGA  >  NC_000913/3806284‑3806573
                                                                                                                                                  |                                                                                                                                               
  cGACCGGAGAAGAAATGCACATTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAATTTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGa                                                                                                                                               <  6:322095/147‑1 (MQ=255)
  cGACAGGATATGAAATGCACATTGCTCCGCACGCCGAGTTTTTCTGCCAGCGGTTCAAATTTTCGCGGCTTATCCTGACGTACCACAAATATAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGa                                                                                                                                               <  3:355804/147‑1 (MQ=255)
   gACCGGAGAAGAAATGCACATTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATc                                                                                                                                             <  4:291789/148‑1 (MQ=255)
    aCCGGAGAAGAAATGCACATTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCt                                                                                                                                            <  2:76907/148‑1 (MQ=255)
       ggAGAAGAAATGCACATTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTaca                                                                                                                                         <  7:354999/148‑1 (MQ=255)
        gagaAGAAATGCACATTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTacac                                                                                                                                        >  1:343411/1‑148 (MQ=255)
            agaaATGCACATTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGGAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCtt                                                                                                                                     >  1:363980/1‑147 (MQ=255)
              aaaTGCACATTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTAc                                                                                                                                  >  4:308650/1‑148 (MQ=255)
                 tGCACATTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGc                                                                                                                               >  4:241906/1‑148 (MQ=255)
                    acaTTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGc                                                                                                                                                 >  1:107554/1‑127 (MQ=255)
                          cTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTg                                                                                                                      <  5:216871/148‑1 (MQ=255)
                            ccGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTAGAGGCTTATCCTGCACAACAACAAATAAAAGCGTATTGTGACGTAAAGAATCCGGTACAGATGCCAAAGCTTCAAGTGAGCGATCTACACCTTTACTGCCAAAAt                                                                                                                         >  6:221829/1‑143 (MQ=255)
                             cGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGa                                                                                                                     <  3:56148/146‑1 (MQ=255)
                               cACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGa                                                                                                                                               <  5:282567/118‑1 (MQ=255)
                               cACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGa                                                                                                                                               >  6:282567/1‑118 (MQ=255)
                                  gCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGAACAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAAcc                                                                                                              >  6:168620/1‑148 (MQ=255)
                                     gAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGc                                                                                                           <  3:273124/148‑1 (MQ=255)
                                      aGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACGTTTACGGCCAAAATCTGATCCAACCTg                                                                                                            >  5:291424/1‑146 (MQ=255)
                                      aGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTg                                                                                                            >  5:74940/1‑146 (MQ=255)
                                       gTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAg                                                                                                         >  8:334583/1‑148 (MQ=255)
                                         ttttCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACAATGCCAAAGATTCAATTGAGCGATCTACACCTTTACGTCCAAAATCTGATCCAACCTGAAGt                                                                                                        >  2:141408/1‑147 (MQ=255)
                                            tCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCAAACCTGCAGtaat                                                                                                     >  7:284378/1‑147 (MQ=255)
                                                         cAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCg                                                                                                                                                <  2:321017/91‑1 (MQ=255)
                                                         cAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCg                                                                                                                                                >  1:321017/1‑91 (MQ=255)
                                                             ttttCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCttt                                                                                    >  8:292873/1‑147 (MQ=255)
                                                             ttctCGCGGCCAATCCTGACCAACAACAAATAAACGCGTATTCTGACGTAATGATTTTTTTTTTTTATTGATAGGTTGAATTGAGAGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCtttt                                                                                   <  6:81521/145‑1 (MQ=255)
                                                                    ggCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCAt                                                                            >  1:307221/1‑148 (MQ=255)
                                                                    ggCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGAAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGATTTGTTGCTCTTTTATGCCAt                                                                            >  2:78383/1‑148 (MQ=255)
                                                                      cTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCAttt                                                                          >  5:378091/1‑148 (MQ=255)
                                                                           ccTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACCCCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTg                                                                     >  1:170740/1‑148 (MQ=255)
                                                                           ccTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTg                                                                     >  4:225579/1‑148 (MQ=255)
                                                                           ccTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCAGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTg                                                                     >  4:116516/1‑148 (MQ=255)
                                                                            cTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCa                                                                                                          >  8:34741/1‑110 (MQ=255)
                                                                            cTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCa                                                                                                          <  7:34741/110‑1 (MQ=255)
                                                                              gACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGctct                                                                                      >  4:325221/1‑128 (MQ=255)
                                                                              gACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCAATTTTTCTGGc                                                                   >  4:290802/1‑147 (MQ=255)
                                                                              gACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATACAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGc                                                                   >  3:70037/1‑147 (MQ=255)
                                                                               aCCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGa                                                                 >  5:188762/1‑148 (MQ=255)
                                                                                  aacaacaaATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTCCACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATa                                                               >  1:347479/1‑147 (MQ=255)
                                                                                  aacaacaaATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCtttt                                                                                   >  4:278997/1‑127 (MQ=255)
                                                                                  aacaacaaATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCtttt                                                                                   <  3:278997/127‑1 (MQ=255)
                                                                                   acaacaaATAAAAGCGTATTGTGACGTAATGATTTTTTTTTTTTATTGAAAGGGTGAATTGAGAGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCAATTTTTCTGGCGATaaa                                                             <  2:363980/148‑1 (MQ=255)
                                                                                   acaacaaATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATaaa                                                             >  7:78804/1‑148 (MQ=255)
                                                                                      acaaATAAAAGCGTATTGTTACGTAATGATTCCTTTTATTATTTGATAGGTTCAATTGAGAGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGcc                                                                              <  8:204455/128‑1 (MQ=255)
                                                                                      acaaATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAAtt                                                           >  5:155018/1‑147 (MQ=255)
                                                                                       caaATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATa                                                               >  2:319408/1‑142 (MQ=255)
                                                                                       caaATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAAttt                                                          >  4:208273/1‑147 (MQ=255)
                                                                                       caaATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAAttt                                                          >  4:270876/1‑147 (MQ=255)
                                                                                         aaTAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCAc                                                       >  6:353437/1‑148 (MQ=255)
                                                                                          aTAAAAGCGTATTGTGACGTAATGATTCTGTTTTTGATGTGATAGGTTGAATTGAGAGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTg                                                                     <  8:102372/133‑1 (MQ=255)
                                                                                          aTAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACg                                                      >  4:305014/1‑148 (MQ=255)
                                                                                           tAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTgg                                                                    >  7:102372/1‑133 (MQ=255)
                                                                                           tAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACgg                                                     >  5:365096/1‑148 (MQ=255)
                                                                                           tAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGACAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTTCTCTTTTATGCCATTTTTCTGGCGATAAATTTAACgg                                                     >  5:196353/1‑148 (MQ=255)
                                                                                               aGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGt                                                 >  6:301824/1‑148 (MQ=255)
                                                                                                gCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGtt                                                <  7:283223/148‑1 (MQ=255)
                                                                                                 cGTATTGTGACGTAATGATTCCGTTATAGATTTCAAAGCTTCAATTGAGAGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGttt                                               <  2:343411/148‑1 (MQ=255)
                                                                                                    aTTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTgg                                             >  2:329844/1‑147 (MQ=255)
                                                                                                    aTTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTgg                                             >  5:78246/1‑147 (MQ=255)
                                                                                                      tgtgACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGAt                                          >  4:250324/1‑148 (MQ=255)
                                                                                                      tgtgACGTAATGATTCCGGTAACGATGCAAAAGCTTCAATTGAGCGATCTACACGTTTACGGCCTAAATCTGATCCAACACGCAGTAATAAGTTTTGTTGGTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGttttgtat                                          >  5:322517/1‑143 (MQ=255)
                                                                                                      tgtgACGTAAGGATTCAGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACATGCAGTAATAAGTTTTGTTTCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGTCTGTTTTGGAt                                          >  7:162285/1‑148 (MQ=255)
                                                                                                          aCGTAATGCTTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGc                                      >  3:307295/1‑148 (MQ=255)
                                                                                                            gTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATCTGCTc                                    <  7:334583/148‑1 (MQ=255)
                                                                                                            gTAATGATTCAGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAAACTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTc                                    <  3:14909/148‑1 (MQ=255)
                                                                                                             tAATGATTCCGGTAACGATGCCAAAGCTTGAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATTAATTTCACGGCTGTTTGGGATTTGCTCa                                   >  2:39126/1‑148 (MQ=255)
                                                                                                             tAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCa                                   <  1:86778/148‑1 (MQ=255)
                                                                                                              aaTGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGAt                                          >  7:167341/1‑140 (MQ=255)
                                                                                                              aaTGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCAc                                  >  8:384148/1‑148 (MQ=255)
                                                                                                              aaTGATTCAGTTTACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTc                                    <  8:154858/146‑1 (MQ=255)
                                                                                                               aTGATTCCTGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCATATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCAc                                  <  1:77001/147‑1 (MQ=255)
                                                                                                               aTGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTAACGGCTGTTTGGGATTTGCTCAca                                 >  7:154858/1‑147 (MQ=255)
                                                                                                                tGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTAACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTATTTTATGCCATTTTTCTGCCGATAAATTTCACGGCTGTTTGGGATTTCCTCACTg                                >  4:355705/1‑148 (MQ=255)
                                                                                                                 gATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTg                                >  8:200482/1‑147 (MQ=255)
                                                                                                                 gATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTg                                >  2:138684/1‑147 (MQ=255)
                                                                                                                 gATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTg                                >  7:289424/1‑147 (MQ=255)
                                                                                                                    tcaggTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAATTCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGACATTTTTCTGGCTATAAATTTCAAGGCTGTTTGGGATTTGCCCACTGTAtt                            >  6:114274/4‑148 (MQ=255)
                                                                                                                    tCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATATGATCAAACCTGCAGTAATAAGTTTTGTTGATCTTTTATGACATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTAtt                            >  7:378303/1‑148 (MQ=255)
                                                                                                                           aCGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTc                     <  1:138684/148‑1 (MQ=255)
                                                                                                                           aCGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTc                     <  3:225579/148‑1 (MQ=255)
                                                                                                                           aCGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTGTGGGATTTGCTCACTGTATTTTCTGTc                     <  3:308650/148‑1 (MQ=255)
                                                                                                                            cGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTcc                    >  2:78359/1‑148 (MQ=255)
                                                                                                                              aTGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCgg                  <  7:312700/148‑1 (MQ=255)
                                                                                                                              aTGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCg                   >  2:303887/1‑147 (MQ=255)
                                                                                                                              aTGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCg                   >  8:227764/1‑147 (MQ=255)
                                                                                                                                  cAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATaa              <  1:329844/148‑1 (MQ=255)
                                                                                                                                   aaaGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCtttt                                                                                   <  6:57936/78‑1 (MQ=255)
                                                                                                                                   aaaGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCAtttt                                                                         <  3:94897/88‑1 (MQ=255)
                                                                                                                                   aaaGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGATGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATaaa             >  4:61032/1‑148 (MQ=255)
                                                                                                                                        ttCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCg        <  2:307221/148‑1 (MQ=255)
                                                                                                                                         tCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCg        >  1:190289/1‑147 (MQ=255)
                                                                                                                                          cAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATAcc         <  2:190289/145‑1 (MQ=255)
                                                                                                                                           aaTTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGGTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTTTTTTCTGTCCGTATAAATACCGgga     <  3:116516/148‑1 (MQ=255)
                                                                                                                                           aaTTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCggg      <  3:23226/147‑1 (MQ=255)
                                                                                                                                            aTTTAGCGATCTACTCCTTTACGGGCAAAATCTGATCCAACCTGCTGTATTCAGTTTTGTTGATCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATGTGCTCACTGTATTTTCTGTCCGGATAAATACCGggag    <  1:316869/148‑1 (MQ=255)
                                                                                                                                             ttGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGgga     <  2:190208/146‑1 (MQ=255)
                                                                                                                                             ttGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGgga     >  1:190208/1‑146 (MQ=255)
                                                                                                                                               gAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGTGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGggagga  <  3:208273/147‑1 (MQ=255)
                                                                                                                                                  cGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACt                                 <  7:376148/113‑1 (MQ=255)
                                                                                                                                                  cGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACt                                 >  8:376148/1‑113 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TGCGACCGGAGAAGAAATGCACATTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGA  >  NC_000913/3806284‑3806573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: