Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,236,541 A→T 5.6% T94S (ACT→TCT)  yjbF → extracellular polysaccharide production lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,236,5410AT 5.6% 209.4 / 3.1 71T94S (ACT→TCT) yjbFextracellular polysaccharide production lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (35/30);  new base T (2/2);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

AAATATGCCCTACGCCAGCCAGTACATGCAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCT  >  NC_000913/4236396‑4236688
                                                                                                                                                 |                                                                                                                                                   
aaaTATGCCCTACGCCAGCCAGTACATGCAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGAct                                                                                                                                                   >  4:45741/1‑148 (MQ=255)
   tatGCCCTACGCCAGCCAGTACATGCAGCTTAATGGCGGGACGCAGTTATTTGTGGTGCTGGCCGTCGCTGAAGACGGACAACAAAAATGGTTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTTGTGAAGACTCTg                                                                                                                                                <  5:72978/148‑1 (MQ=255)
     tGCCCTACGCCAGCCAGTACATGCAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGc                                                                                                                                               <  7:218992/147‑1 (MQ=255)
         cTACGCCAGCCAGTACATGCGGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTcggc                                                                                                                                          >  2:259774/1‑148 (MQ=255)
                agccagTACATGCAGCTTAATGGCGCGCCGCAGATATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGAc                                                                                                                                    >  2:262637/1‑147 (MQ=255)
                      tACATGCAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGa                                                                                                                             <  4:126206/148‑1 (MQ=255)
                       aCATGCAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGAt                                                                                                                            >  8:124191/1‑148 (MQ=255)
                        cATGCAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGAt                                                                                                                            <  5:210848/147‑1 (MQ=255)
                          tGCAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGa                                                                                                                         <  7:124191/148‑1 (MQ=255)
                           gCAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTg                                                                                                                          >  3:153590/1‑146 (MQ=255)
                                 tAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGaa                                                                                                                   >  3:162015/1‑147 (MQ=255)
                                      gctggCCTCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGTACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCt                                                                                                             <  4:153590/145‑1 (MQ=255)
                                      gCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGTCTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCt                                                                                                             >  6:333094/1‑148 (MQ=255)
                                      gCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCt                                                                                                             <  3:360551/148‑1 (MQ=255)
                                         ggCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTtgc                                                                                                          <  3:255747/148‑1 (MQ=255)
                                           ccGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACCACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTTGTGAAGACTCTGCTCGTCGGCGACAGCCTGATTGAAGTGAATAACCTtgctg                                                                                                        >  6:148881/1‑148 (MQ=255)
                                           ccGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTg                                                                                                                     <  7:368405/135‑1 (MQ=255)
                                           ccGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTg                                                                                                                     >  8:368405/1‑135 (MQ=255)
                                                 ttATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCt                                                                                                                               >  5:130114/1‑119 (MQ=255)
                                                 ttATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCt                                                                                                                               <  6:130114/119‑1 (MQ=255)
                                                  tATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGCCTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGAccc                                                                                                 >  5:244180/1‑148 (MQ=255)
                                                  tATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGAccc                                                                                                 >  3:74162/1‑148 (MQ=255)
                                                        tggtgCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGc                                                                                               <  4:91715/144‑1 (MQ=255)
                                                        tggtgCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGc                                                                                               >  3:91715/1‑144 (MQ=255)
                                                            gCTGGCCTTCGCTGAAGACGGACAACAAAAATGCGTCACTCAGCATCAGGCTACTCTCGTTACCCAACATCGCCGTCTGGTGAAGACTCTGCTCGGCCCCCACAACCAGATAGAAGTGAATAACCATGCAGCAGACCCGCTGATTaaa                                                                                       >  8:166030/1‑148 (MQ=255)
                                                             cTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAAc                                                                                      >  5:66617/1‑148 (MQ=255)
                                                               ggCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTTATTGAAGTGAATAACCTTGCTGCCGAc                                                                                                   <  4:213376/133‑1 (MQ=255)
                                                               ggCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGAc                                                                                                   >  3:213376/1‑133 (MQ=255)
                                                                gCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCg                                                                                   >  4:45692/1‑148 (MQ=255)
                                                                 ccTTCGCTGAAGACGGACAACAAAAATGGGGCACTCAGGATCAGGCTACTCTCTTTAACCAACATGTCCGTCTGGTGAAGACTCTGCTCGTAGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGc                                                                                  <  3:213956/148‑1 (MQ=255)
                                                                      gCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACaaa                                                                             <  3:45692/148‑1 (MQ=255)
                                                                        tGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATc                                                                           >  5:331625/1‑148 (MQ=255)
                                                                          aaGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGAGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCg                                                                          <  7:166030/147‑1 (MQ=255)
                                                                            gACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTg                                                                       <  3:45741/148‑1 (MQ=255)
                                                                             aCGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGa                                                                      <  8:277155/148‑1 (MQ=255)
                                                                             aCGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGa                                                                      >  7:277155/1‑148 (MQ=255)
                                                                              cGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGAt                                                                     <  4:31553/148‑1 (MQ=255)
                                                                                 acaacaAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGTCGCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGgc                                                                  <  5:333094/148‑1 (MQ=255)
                                                                                   aacaaAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACACAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGTCACCCTGATTTAACCCGCTCAAATCGTTGATGgcg                                                                 >  5:87604/1‑147 (MQ=255)
                                                                                   aacaaAAATGGGTCACTCAGGATCAGGCTACTCTCGTTAACCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGgcg                                                                 >  3:212390/1‑147 (MQ=255)
                                                                                    acaaAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGCATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATgcgc                                                                 >  4:358547/1‑143 (MQ=255)
                                                                                       aaaaTGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGc                                                            >  4:356746/1‑148 (MQ=255)
                                                                                       aaaaTGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGc                                                            >  8:202205/1‑148 (MQ=255)
                                                                                         aaTGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTaa                                                                                        <  2:50603/118‑1 (MQ=255)
                                                                                         aaTGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTaa                                                                                        >  1:50603/1‑118 (MQ=255)
                                                                                                    cAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGtt                                                                        >  2:62984/1‑123 (MQ=255)
                                                                                                    cAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGtt                                                                        <  1:62984/123‑1 (MQ=255)
                                                                                                    cAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACGa                                               >  1:130440/1‑148 (MQ=255)
                                                                                                     aGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACg                                                <  7:75278/146‑1 (MQ=255)
                                                                                                     aGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACGCGGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACg                                                <  7:378551/146‑1 (MQ=255)
                                                                                                       gATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACaaa                                                                             >  8:21283/1‑115 (MQ=255)
                                                                                                       gATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACaaa                                                                             <  7:21283/115‑1 (MQ=255)
                                                                                                        aTCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACGATggg                                           <  6:331625/148‑1 (MQ=255)
                                                                                                                 ctctCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGCCCgc                                  >  5:94715/1‑147 (MQ=255)
                                                                                                                 ctctCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCg                                   <  1:259774/147‑1 (MQ=255)
                                                                                                                   ctcGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTg                                                                       >  7:136943/1‑109 (MQ=255)
                                                                                                                   ctcGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTg                                                                       <  8:136943/109‑1 (MQ=255)
                                                                                                                      gTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGt                                <  7:202205/145‑1 (MQ=255)
                                                                                                                       ttACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTAc                              >  2:176226/1‑146 (MQ=255)
                                                                                                                        tACCCAACATGGCCGTCTGGTGAAGACTCTGCTCTGCGGCGACAACCTGATTGAAGTTAATAACCTTTCTGCCTACCCGCTGATTAAACCCGCACAACTCGTTGATGGAGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACcag                           <  7:38776/148‑1 (MQ=255)
                                                                                                                           ccAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACcagcag                        >  2:369020/1‑148 (MQ=255)
                                                                                                                           acAACATGGCAGTCTGGTGAAGCCTCTGAGAGGCGGCGACAACCTGATTTAAGTGAATAACATTGATGCCGACCCGCTGATTAAACCATCACAAATCGTTGATGGAGCAATCTGGACGAGAAGGAGGGGCTGTAAAGAGTACaaga                          >  8:38776/2‑145 (MQ=255)
                                                                                                                               cATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGAt                                                                                                                            <  5:118343/44‑1 (MQ=255)
                                                                                                                               cATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGAt                                                                                                                            >  6:118343/1‑44 (MQ=255)
                                                                                                                               cATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGCCGCGCACGATGGGCTGGACCGAGTACCAGCAGGtac                    >  6:2125/1‑148 (MQ=255)
                                                                                                                                aTGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGtacg                   <  1:262637/148‑1 (MQ=255)
                                                                                                                                      gTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGtacgctacgc             >  5:56931/1‑148 (MQ=255)
                                                                                                                                          ggTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCAcc         >  6:227361/1‑148 (MQ=255)
                                                                                                                                            tGAAGTCTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCg                                   <  4:29748/120‑1 (MQ=255)
                                                                                                                                            tGAAGTCTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCg                                   >  3:29748/1‑120 (MQ=255)
                                                                                                                                              aaGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCAc     >  2:148600/1‑148 (MQ=255)
                                                                                                                                                 aCTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGCCCCGCTGATTACCCCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCCGCAGGTACGCTACGCCACCGCACGcg  >  2:241618/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AAATATGCCCTACGCCAGCCAGTACATGCAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAAACCCGCACAAATCGTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCT  >  NC_000913/4236396‑4236688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: