Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,463,293 T→A 6.0% T473S (ACA→TCA)  treC ← trehalose‑6‑P hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,463,2930TA 6.0% 205.6 / 3.0 67T473S (ACA→TCA) treCtrehalose‑6‑P hydrolase
Reads supporting (aligned to +/- strand):  ref base T (31/31);  new base A (2/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

TTGCCGCGCATTTGCCCTGCCTGCCAGGGTTGGATCTCACGGCTAAGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGATAGTTATCGCCCAGGCCAATCCACGGTTCGCCAGCCGTAAACCCGGCAT  >  NC_000913/4463151‑4463439
                                                                                                                                              |                                                                                                                                                  
ttGCCGCGCATTTGCCCTGCCTGCCAGGGTTGGATCTCACGGCTAAGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGa                                                                                                                                               <  1:181852/148‑1 (MQ=255)
      cgcATTTGCCCTGCCTGCCAGGGTTGGATCTCACGGCTAAGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCtt                                                                                                                                         <  5:132705/148‑1 (MQ=255)
          tttGCCCTGCCTGCCAGGGTTGGATCTCACGGCTAAGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTg                                                                                                                                     <  3:30145/148‑1 (MQ=255)
           ttGCCCTGCCTGCCAGGGTTGGATCTCACGGCTAAGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAgg                                                                                                                                                >  2:352961/1‑136 (MQ=255)
           ttGCCCTGCCTGCCAGGGTTGGATCTCACGGCTAAGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAgg                                                                                                                                                <  1:352961/136‑1 (MQ=255)
           ttGCCCTGCCTGCCAGGGTTGGATCTCACGGCTAAGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGc                                                                                                                                    >  1:81675/1‑148 (MQ=255)
            tGCCCTGCCTGCCAGGGTTGGATCTCACGGCTAAGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGc                                                                                                                                    <  4:25830/147‑1 (MQ=255)
              cccTGCCTGCCAGGGTTGGATCTCACGGCTAAGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCtt                                                                                                                                  <  4:151020/147‑1 (MQ=255)
                  gcctgccAGGGTTGGATCTCACGGCTAAGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATGCTGGTAATTGCCCCATGACAGGATGGCTTCCTGCTTACg                                                                                                                               >  3:175923/1‑146 (MQ=255)
                  gcctgccAGGGTTGGATCTCACGGCTAAGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACg                                                                                                                               >  1:236132/1‑146 (MQ=255)
                  gcctgccAGGGTTGGATCTCACGGCTAAGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACg                                                                                                                               >  4:214756/1‑146 (MQ=255)
                   cctgccAGGGTTGGATCTCACGGCTAAGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGc                                                                                                                              <  8:56664/146‑1 (MQ=255)
                                     cACGGCTAAGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTgg                                                                                                            >  6:220074/1‑146 (MQ=255)
                                     cACGGCTAAGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTgg                                                                                                            >  3:327238/1‑146 (MQ=255)
                                      aCGGCTAAGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGt                                                                                                           <  3:30858/146‑1 (MQ=255)
                                      aCGGCTAAGGTGGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCGGGTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTa                                                                                                          <  4:65610/147‑1 (MQ=255)
                                            aaGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTa                                                                                                                    <  5:71225/131‑1 (MQ=255)
                                            aaGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTa                                                                                                                    >  2:12274/1‑131 (MQ=255)
                                            aaGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTa                                                                                                                    >  6:71225/1‑131 (MQ=255)
                                            aaGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTa                                                                                                                    <  1:12274/131‑1 (MQ=255)
                                            aaGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTa                                                                                                    >  6:110506/1‑147 (MQ=255)
                                             aGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTaaa                                                                                                  <  1:123643/148‑1 (MQ=255)
                                                         aCCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGaa                                                                                       <  5:220074/147‑1 (MQ=255)
                                                           cAGCAAGGTATGCCCCATCCATTAACGCCGATAGCACCACAAAACAGGGCTGTATGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAatca                                                                                    <  3:44649/148‑1 (MQ=255)
                                                            aGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAatcat                                                                                   <  3:166554/148‑1 (MQ=255)
                                                            aGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAatcat                                                                                   >  2:341959/1‑148 (MQ=255)
                                                               aaGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCgg                                                                                <  3:243482/148‑1 (MQ=255)
                                                                aGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCATCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGc                                                                               <  6:115421/148‑1 (MQ=255)
                                                                  gTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGc                                                                               >  2:287546/1‑146 (MQ=255)
                                                                   tttGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAg                                                                            <  5:110506/148‑1 (MQ=255)
                                                                       ccccTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGcgcg                                                                        >  2:127026/1‑148 (MQ=255)
                                                                         ccTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGACAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAgg                                                                                                        >  5:71287/1‑114 (MQ=255)
                                                                         ccTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGACAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAgg                                                                                                        <  6:71287/114‑1 (MQ=255)
                                                                            tCCATTCACGGCGATAGCCCCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAACCACCGAGGAATCATCGGCCAGCGCGGCTTc                                                                   >  3:233800/1‑148 (MQ=255)
                                                                            tCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTc                                                                   >  7:250246/1‑148 (MQ=255)
                                                                             ccATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCtt                                                                    <  4:327238/146‑1 (MQ=255)
                                                                              cATTCACGGCGATAGCACCACAATACAGGTCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGGCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCt                                                                  <  4:102314/147‑1 (MQ=255)
                                                                                ttCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTGATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGa                                                                                           >  2:3154/1‑120 (MQ=255)
                                                                                ttCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTGATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGa                                                                                           <  1:3154/120‑1 (MQ=255)
                                                                                    cGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGa                                                           >  5:230429/1‑148 (MQ=255)
                                                                                      gCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGAtt                                                         <  5:353213/148‑1 (MQ=255)
                                                                                        gATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATttg                                                       <  6:230429/148‑1 (MQ=255)
                                                                                        gATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATttg                                                       >  8:15351/1‑148 (MQ=255)
                                                                                        gATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATttg                                                       <  2:81675/148‑1 (MQ=255)
                                                                                         aTAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATttgt                                                      <  1:341959/148‑1 (MQ=255)
                                                                                          tAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCCGCTTCTACGTTGATttgtt                                                     >  4:148173/1‑148 (MQ=255)
                                                                                           aGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATttgtt                                                     >  5:200373/1‑147 (MQ=255)
                                                                                           aGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATttgtt                                                     >  5:294202/1‑147 (MQ=255)
                                                                                               ccacAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGATAg                                                >  3:290236/1‑148 (MQ=255)
                                                                                               ccacAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGAGTTGTTGATAg                                                >  7:191166/1‑148 (MQ=255)
                                                                                                   aaTACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGATAGTTAt                                            >  7:163038/1‑148 (MQ=255)
                                                                                                    aTACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGt                                                              <  8:262523/129‑1 (MQ=255)
                                                                                                    aTACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGt                                                              >  7:262523/1‑129 (MQ=255)
                                                                                                    aTACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGATAGTTATc                                           >  7:177542/1‑148 (MQ=255)
                                                                                                       cAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGATAGTTATCGc                                         <  2:236132/147‑1 (MQ=255)
                                                                                                        aGGTCTGTTTGGCAGCTGATCCTGGTAATTGCCCCATGTCAGGATGGATTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGATAGTTATCGccc                                       <  6:200373/148‑1 (MQ=255)
                                                                                                        aGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGATAGTTATCGccc                                       <  6:294202/148‑1 (MQ=255)
                                                                                                        aGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGATAGTTATCGccc                                       <  3:148173/148‑1 (MQ=255)
                                                                                                           gCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGATAGTTATCGCCCAgg                                    <  1:383936/148‑1 (MQ=255)
                                                                                                                    cagcagATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGATAGTTATCGCCCCGGCCAATCCAc                           >  1:213520/1‑148 (MQ=255)
                                                                                                                          aTCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCTATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGATAGTTATCGCCCAGGCCAATCCACGGTTCg                     >  8:308894/1‑148 (MQ=255)
                                                                                                                               ggTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAAATTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGATAGTTATCGCCCAGGCCAATCCACGGTTCGCCAGc                >  8:220282/1‑148 (MQ=255)
                                                                                                                                     tGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGATAGTTATCGCCCAGGCCAATCCACGGTTCGCCAGCCGTaaa          >  4:168762/1‑148 (MQ=255)
                                                                                                                                         ccATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGa                                                   <  3:42327/103‑1 (MQ=255)
                                                                                                                                         ccATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGa                                                   >  4:42327/1‑103 (MQ=255)
                                                                                                                                           aTGACAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGATAGTTATCGCCCAGGCCAATCCACGGTTCGCCAGCCGTAAACCCGGc    <  4:175923/148‑1 (MQ=255)
                                                                                                                                             gTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGATAGTTATCGCCCAGGCCAATCCACGGTTCGCCAGCCGTAAACCCGGCAt  >  7:28043/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
TTGCCGCGCATTTGCCCTGCCTGCCAGGGTTGGATCTCACGGCTAAGGTTGGCAATGACCAGCAAGGTTTGCCCCTTCCATTCACGGCGATAGCACCACAATACAGGGCTGTTTGGCAGCAGATCCTGGTAATTGCCCCATGTCAGGATGGCTTCCTGCTTACGCAGTGCGATTAACTTTTGGTAGGTGTAAAACACCGAGGAATCATCGGCCAGCGCGGCTTCTACGTTGATTTGTTGATAGTTATCGCCCAGGCCAATCCACGGTTCGCCAGCCGTAAACCCGGCAT  >  NC_000913/4463151‑4463439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑