Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 2,868,098 IS5 (+) +4 bp coding (653‑656/1140 nt) nlpD ← activator of AmiC murein hydrolase activity, lipoprotein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1427599 =NA (NA)117 (0.980) 86/280 0.2 100% noncoding (1196/1196 nt) IS5 repeat region
?NC_000913 2868098 = 0 (0.000)coding (656/1140 nt) nlpD activator of AmiC murein hydrolase activity, lipoprotein
* ? NC_000913 = 1428794NA (NA)90 (0.760) 71/278 0.6 100% noncoding (1/1196 nt) IS5 repeat region
?NC_000913 = 2868101 0 (0.000)coding (653/1140 nt) nlpD activator of AmiC murein hydrolase activity, lipoprotein

Only 100 of 215 total aligned reads displayed.

CACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACC  >  NC_000913/2867950‑2868246
                                                                                                                                                    |                                                                                                                                                    
aaaaccggaaataacgccagaaatggtggaaaaaatagcctaaataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttcct                                                                                                                                                      >  1:154536‑M2/149‑149 (MQ=255)
 aaaccggaaataacgccagaaatggtggaaaaaatagcctaaataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttcctt                                                                                                                                                     <  6:334009‑M2/2‑1 (MQ=255)
 aaaccggaaataacgccagaaatggtggaaaaaatagcctaaataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttcct                                                                                                                                                      >  1:470005‑M2/148‑148 (MQ=255)
  aaccggaaataacgccagaaatggtggaaaaaatagcctaaataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTa                                                                                                                                                    <  2:154536‑M2/3‑1 (MQ=255)
       gaaataacgccagaaatggtggaaaaaatagcctaaataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCAct                                                                                                                                               >  4:47435‑M2/142‑149 (MQ=255)
         cTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaag                                                                                                                                              <  6:489979‑M2/148‑6 (MQ=255)
          atcaccccagaaatggcggaaaaactagccaaaataggcggattcgatgggtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTg                                                                                                                                            <  8:492774‑M2/11‑1 (MQ=255)
          ataacgccagaaatggtggaaaaaatagcctaaataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTg                                                                                                                                            <  8:306477‑M2/11‑1 (MQ=255)
           taacgccagaaatggtggaaaaaatagcctaaataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGt                                                                                                                                           <  6:293069‑M2/12‑1 (MQ=255)
           taacgccagaaatggtggaaaaaatagcctaaataggctgattcgatgtgttagcgggaaaaaaatcggcccagatcctcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcataatcgaaccttccTTACAACACTGt                                                                                                                                           >  1:168033‑M2/138‑149 (MQ=255)
            aGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtg                                                                                                                                           >  5:292902‑M2/1‑140 (MQ=255)
              tCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcga                                                                                                                                        >  4:49366‑M2/1‑138 (MQ=255)
              tCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcg                                                                                                                                         <  2:359992‑M2/148‑11 (MQ=255)
               gccagaaatggtggaaaaaatagcctaaataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCAc                                                                                                                                       >  4:445753‑M2/134‑149 (MQ=255)
               gccagaaatggtctaaaaaatagcctaaatagtctgaatcgatgtgtttgctggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCAc                                                                                                                                       <  3:65067‑M2/16‑1 (MQ=255)
                 cagaaatggtggaaaaaatagcctaaataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGt                                                                                                                                           <  7:284970‑M2/12‑1 (MQ=255)
                 cagaaatggtggaaaaaatagcctaaataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCAccc                                                                                                                                     >  3:328184‑M2/132‑149 (MQ=255)
                   cAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaag                                                                                                                                   <  3:61351‑M2/149‑17 (MQ=255)
                     aatggtggaaaaaatagcctaaataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGaa                                                                                                                                  <  7:73328‑M2/21‑1 (MQ=255)
                          aGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctga                                                                                                                            >  8:42840‑M2/1‑126 (MQ=255)
                            gaaaaaatagcctaaataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAg                                                                                                                           >  4:136311‑M2/121‑148 (MQ=255)
                            gaaaaaatagcctaaataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAg                                                                                                                           <  3:445753‑M2/28‑1 (MQ=255)
                                aaatagcctaaataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATAc                                                                                                                      <  2:452468‑M2/33‑1 (MQ=255)
                                   tAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatc                                                                                                                   >  1:274138‑M2/1‑117 (MQ=255)
                                      gCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagat                                                                                                                    >  2:337065‑M2/1‑114 (MQ=255)
                                      cctaaataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAAtt                                                                                                                <  4:239571‑M2/39‑1 (MQ=255)
                                        taaataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGt                                                                                                              >  3:223275‑M2/109‑149 (MQ=255)
                                         tACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcata                                                                                                             >  5:73483‑M2/1‑111 (MQ=255)
                                          aataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGt                                                                                                              <  8:57192‑M2/41‑1 (MQ=255)
                                          aataggctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCg                                                                                                            >  4:347441‑M2/107‑149 (MQ=255)
                                            tagtctgattcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatctgcgtgtcagcttgggaagaattgagctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGt                                                                                                          <  2:168033‑M2/45‑1 (MQ=255)
                                              gTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattc                                                                                                          >  3:394088‑M2/1‑106 (MQ=255)
                                              gTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaagg                                                                                                                                             >  5:50606‑M2/1‑106 (MQ=255)
                                              gTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaagg                                                                                                                                             <  6:50606‑M2/112‑7 (MQ=255)
                                              gTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGg                                                                                                                                                  <  8:405676‑M2/107‑2 (MQ=255)
                                                    ttcgatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGc                                                                                                  <  2:90039‑M2/53‑1 (MQ=255)
                                                     tACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagc                                                                                                 >  6:202787‑M2/1‑99 (MQ=255)
                                                       gatgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCaa                                                                                                >  6:372919‑M2/94‑148 (MQ=255)
                                                        atgtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACa                                                                                              <  5:254732‑M2/57‑1 (MQ=255)
                                                          gtgtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAg                                                                                             >  3:181268‑M2/91‑148 (MQ=255)
                                                           tgACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTa                                                                                                                                                    >  5:226354/1‑92 (MQ=255)
                                                           tgACAGTCGGCTCGGTAGAGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACAGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTATgtaaggtgcgaacaatttccagataagagataatcatatacatcaagagcgcatcc                                                                                           <  2:478912‑M2/149‑57 (MQ=255)
                                                            gtttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCaa                                                                                          >  5:4109‑M2/89‑149 (MQ=255)
                                                            gACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatat                                                                                                                         >  1:236975‑M2/1‑92 (MQ=255)
                                                            gACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatat                                                                                                                         <  2:244203‑M2/118‑27 (MQ=255)
                                                             tttgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAAc                                                                                         >  3:69952‑M2/88‑149 (MQ=255)
                                                             aCAGTCGGCTCGGTTGTGCTTGCTGTTTGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCTACATTTTGTTAGggaaggtgctaacaagttcctgatatgagatcatcatattcatccggagcgcatcc                                                                                           >  5:132957‑M2/1‑91 (MQ=255)
                                                              cAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTCAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatacggagcgcatcc                                                                                           <  1:417619‑M2/146‑57 (MQ=255)
                                                               tgcgggaaaaaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACg                                                                                        <  2:68942‑M2/63‑1 (MQ=255)
                                                               aGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccaga                                                                                       >  4:438635‑M2/1‑89 (MQ=255)
                                                                 tCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccagagg                                                                                     >  1:456143‑M2/1‑87 (MQ=255)
                                                                      tCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccagagggacat                                                                                <  4:276791‑M2/149‑68 (MQ=255)
                                                                       aaaaatcgacccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAAttt                                                                               <  8:448305‑M2/72‑1 (MQ=255)
                                                                        aaaatcggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAAtttt                                                                              <  4:305573‑M2/73‑1 (MQ=255)
                                                                        aaaatcggccacgatccgctaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgaaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGGCGGTTGCGACGCAACAGCAACGGTGGAAtttt                                                                              <  6:389344‑M2/73‑1 (MQ=255)
                                                                          ttGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccagagggacatcat                                                                             <  7:249007‑M2/148‑71 (MQ=255)
                                                                            gtgCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccagagggacatcatga                                                                           >  8:249007‑M2/1‑76 (MQ=255)
                                                                            gtgCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccagaggga                                                                                   <  8:474661‑M2/140‑65 (MQ=255)
                                                                              ggcccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCATCGGTGGAATTTTGTGCa                                                                         <  1:51991‑M2/78‑1 (MQ=255)
                                                                               cttgctGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatc                                                                                                                >  5:10590‑M2/1‑73 (MQ=255)
                                                                                cccagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGtt                                                                                                         >  1:329205‑M2/69‑114 (MQ=255)
                                                                                  cagatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCtt                                                                    <  3:136311‑M2/83‑1 (MQ=255)
                                                                                    tGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccagagggacatcatgagccatcaac                                                                  <  7:452324‑M2/149‑82 (MQ=255)
                                                                                    gatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCaa                                                                                                >  1:108600‑M2/65‑119 (MQ=255)
                                                                                    gatccgcgaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCaa                                                                                                <  2:108600‑M2/55‑1 (MQ=255)
                                                                                          tACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccagagggacatcatgagccatcaactcacct                                                            <  7:423046‑M2/149‑88 (MQ=255)
                                                                                           gaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTTGAATTTTGTGCAGGCTTGATCACAAc                                                           <  7:263623‑M2/92‑1 (MQ=255)
                                                                                           gaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAAc                                                           <  4:69952‑M2/92‑1 (MQ=255)
                                                                                           gaaattttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTGCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACaa                                                            >  6:145737‑M2/58‑148 (MQ=255)
                                                                                               ttttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGc                                                                      <  1:90720‑M2/81‑1 (MQ=255)
                                                                                               ttttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACaa                                                            <  2:211210‑M2/91‑1 (MQ=255)
                                                                                               ttttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACaa                                                            >  1:211210‑M2/54‑144 (MQ=255)
                                                                                                 ttaatcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCtt                                                      <  3:347441‑M2/97‑1 (MQ=255)
                                                                                                   aGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccagagggacatcatgagccatcaactcaccttcgccga                                                     <  4:38373‑M2/147‑95 (MQ=255)
                                                                                                    atcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGct                                                   <  4:181268‑M2/100‑1 (MQ=255)
                                                                                                    atcagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCAATCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGctc                                                  <  2:470005‑M2/101‑1 (MQ=255)
                                                                                                     gcgcTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccagagggacatcatgagccatcaactcaccttcgccgatagt                                                 <  3:303837‑M2/149‑99 (MQ=255)
                                                                                                      cagcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTctg                                                >  6:108470‑M2/47‑149 (MQ=255)
                                                                                                       agcgagtcagcttgggaagaaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTctgc                                               >  1:473391‑M2/46‑149 (MQ=255)
                                                                                                          gtgACCGTGGTCGCAGTTGGCTTGTTGTTCGCCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccagagggacatcatgagccatcaactcaccttcgcggatagtgaatt                                            <  5:460661‑M2/149‑104 (MQ=255)
                                                                                                             aCCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccagagggacatcatgagccatcaactcaccttcgccgata                                                   <  3:297043‑M2/139‑97 (MQ=255)
                                                                                                             aCCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccagagggacatcatgagccatcaactcaccttcgccgata                                                   >  4:297043‑M2/1‑43 (MQ=255)
                                                                                                                     cGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccagagggacatc                                                                               <  7:133558‑M2/103‑69 (MQ=255)
                                                                                                                          aaatgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGGGGAATTTTGTGCAGGCTTGATCACAACTCCGTGCTCGGCTGCGTCGGCCTGGGTAATg                            >  1:422541‑M2/27‑149 (MQ=255)
                                                                                                                            atgacctgcttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCa                         >  5:368838‑M2/25‑149 (MQ=255)
                                                                                                                                    gttCGGCAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccagagggacatcatgagccatcaactcaccttcgccgatagtgaattcagcac                                      <  2:98718‑M2/129‑110 (MQ=255)
                                                                                                                                    cttattcgcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGc                  <  2:192247‑M2/133‑1 (MQ=255)
                                                                                                                                        ggCAACATTTTGTTAGggaatgtgcgaacaagttcctgatatgagatcagcattttcatccggagagcatcccagagggagatcatgtgccatcaactcaccttcgccgatagtgaattcagcactaagcgccgtcagacccgaaaata              >  5:160753‑M2/1‑16 (MQ=255)
                                                                                                                                          cAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccagagggacatcatgagccatcaactcaccttcgccgatagtggattcagcactaagcgccgtcagacccgaaaa                <  1:368373‑M2/145‑132 (MQ=255)
                                                                                                                                          cAACATTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccagagggacatcatgagccatcaactcaccttcgccgatagtgaattcagcactaagcgccgtcagacccgaaaa                <  3:14332‑M2/145‑132 (MQ=255)
                                                                                                                                           gcaccttccTTAGCACTCTGTTCACCCGAAGACTCAGAAAACGTAATTGTCCGTTGCGACGCAACAGCAACGGAGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGGGAt           >  4:120022‑M2/10‑149 (MQ=255)
                                                                                                                                            caccttccTTAGAAATCTGTTAACCCGAAGACTCAGAATTCGTAATTGTCGGTTGCGAAGCAACAGCAAAGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGAtt          <  8:258251‑M2/141‑1 (MQ=255)
                                                                                                                                             accttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTg         >  7:344475‑M2/8‑149 (MQ=255)
                                                                                                                                             accttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTg         >  8:107186‑M2/8‑149 (MQ=255)
                                                                                                                                              aTTTTGTTAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccgtagcgcatccctgagggacatcatgagccatcaactcaccttcgccgatagtgaattcagcactaagcgccgtcagacccgaaaagagatttt        >  3:474036‑M2/1‑10 (MQ=255)
                                                                                                                                               cttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGc       >  6:252054‑M2/6‑149 (MQ=255)
                                                                                                                                                ttccTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCg      >  2:435944‑M2/5‑149 (MQ=255)
                                                                                                                                                   cTTAGCACTCTGTGCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc   <  5:120501‑M2/148‑1 (MQ=255)
                                                                                                                                                    ttAGggaaggtgcgaacaagttcctgatatgagatcatcatattcatccggagcgcatcccagagggacatcatgagccatcaactcaccttcgccgatagtgaattcagcactaagcgccgtcagacccgaaaagagattttcctctc  >  3:38373‑M2/1‑4 (MQ=255)
                                                                                                                                                    ttAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAcc  >  8:276978/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTGTGACCGTGGTCGCAGTTGGCTTGTTGTTCGGCAACATTTTGTTAGCACTCTGTTCACCCGAAGACTCAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACC  >  NC_000913/2867950‑2868246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.