Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,315,699	G→T	D738Y (GAT→TAT)	rcsD →	phosphotransfer intermediate protein in two‑component regulatory system with RcsBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,315,699	0	G	T	100.0%	341.9 / NA	96	D738Y (GAT→TAT)	rcsD	phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (48/48);  total (48/48)

TTTACTGGATGATGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGC  >  NC_000913/2315554‑2315843                                                                                                                                                  |                                                                                                                                                 tttACTGGATGATGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGtata                                                                                                                                               <  5:413910/149‑1 (MQ=255) tttACTGGATGATGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGtata                                                                                                                                               <  8:101759/149‑1 (MQ=255) tttACTGGATGATGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTAt                                                                                                                                                >  8:244985/1‑148 (MQ=255) tttACTGGATGATGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTAt                                                                                                                                                >  1:324604/1‑148 (MQ=255)   tACTGGATGATGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATaa                                                                                                                                              <  6:271387/148‑1 (MQ=255)   tACTGGATGATGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAAt                                                                                                                                             >  4:238281/1‑149 (MQ=255)      tGGATGATGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCg                                                                                                                                          >  2:375652/1‑149 (MQ=255)       ggATGATGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGt                                                                                                                                         >  8:43155/1‑149 (MQ=255)        gatgatGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTc                                                                                                                                        >  1:241477/1‑149 (MQ=255)        gatgatGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACATGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTc                                                                                                                                        >  7:239796/1‑149 (MQ=255)         atgatgTCTGCGTAATAGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTc                                                                                                                                        >  8:171898/1‑148 (MQ=255)               tCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCtt                                                                                                                                 <  2:241477/149‑1 (MQ=255)               tCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCt                                                                                                                                  <  8:107593/148‑1 (MQ=255)                   cGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTa                                                                                                                                <  1:375652/146‑1 (MQ=255)                   cGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTa                                                                                                                                <  6:342052/146‑1 (MQ=255)                   cGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACt                                                                                                                              <  4:151704/148‑1 (MQ=255)                   agTAATGGTGGATTTTACTTAGGCAGAAATTCTGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACACGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTAc                                                                                                                               <  2:294108/146‑1 (MQ=255)                          gtggATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTgg                                                                                                                      >  3:276931/1‑149 (MQ=255)                              aTGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGgcttg                                                                                                                  <  3:238281/149‑1 (MQ=255)                               tGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGgcttgc                                                                                                                 >  1:294878/1‑149 (MQ=255)                                 ttACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACACTATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGgcttgctt                                                                                                               >  1:266267/1‑149 (MQ=255)                                   aCTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTa                                                                                                                                >  1:411345/1‑130 (MQ=255)                                   aCTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTa                                                                                                                                <  2:411345/130‑1 (MQ=255)                                   aCTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCttt                                                                                                              >  5:44413/1‑148 (MQ=255)                                    attCTGCAGATATTCTGATTATTTTAACTCTCCAGTTAGAAAATTGGTGTTCAACCTGTATCACACCCGATTAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACTTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCtttt                                                                                                             <  8:239796/147‑1 (MQ=255)                                     ttCGGCAGAAATTCGGAATTTTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATTAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGcc                                                                                                                           <  5:370304/133‑1 (MQ=255)                                     ttCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTa                                                                                                                                >  7:175968/1‑128 (MQ=255)                                     ttCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTa                                                                                                                                <  8:175968/128‑1 (MQ=255)                                     ttCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTg                                                                                                                       <  3:189603/137‑1 (MQ=255)                                     ttCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTg                                                                                                                       >  4:189603/1‑137 (MQ=255)                                       cGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTaa                                                                                                           <  2:324604/147‑1 (MQ=255)                                         gCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCga                                                                                                       <  2:228520/149‑1 (MQ=255)                                            gAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCgatga                                                                                                    <  7:171898/149‑1 (MQ=255)                                            gAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCgatga                                                                                                    <  2:28317/149‑1 (MQ=255)                                            gAAATTCGGAATAATGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTTATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCgatga                                                                                                    <  8:404638/149‑1 (MQ=255)                                             aaaTTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCgatgat                                                                                                   <  7:43155/149‑1 (MQ=255)                                                 tCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTgg                                                                                                                      >  2:232759/1‑126 (MQ=255)                                                 tCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTgg                                                                                                                      <  1:232759/126‑1 (MQ=255)                                                   ggAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAggc                                                                                              <  2:156331/148‑3 (MQ=255)                                                       tatTGTCACTCGCCAGTTAGAAAATTTGGGTGCAACCTGTATCCCACCCGATGAAAGATTAATTTGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCg                                                                                         >  2:218962/1‑149 (MQ=255)                                                            tCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACgg                                                                                    >  7:40012/1‑149 (MQ=255)                                                                tCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGaaa                                                                                >  4:313905/1‑149 (MQ=255)                                                                  gCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATTATGAGTCTGGCGTACGGGaaa                                                                                <  7:244985/147‑1 (MQ=255)                                                                   ccAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTaa                                                                                                           >  6:71563/1‑119 (MQ=255)                                                                   ccAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTaa                                                                                                           <  5:71563/119‑1 (MQ=255)                                                                       ttAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGcc                                                                         >  1:158886/1‑149 (MQ=255)                                                                         aGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCt                                                                        <  4:252562/148‑1 (MQ=255)                                                                         aGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTg                                                                       >  2:95743/1‑149 (MQ=255)                                                                           aaaaTTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGt                                                                     <  3:313905/149‑1 (MQ=255)                                                                            aaaTTGGGGTGCAACCTGTGTCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGt                                                                     >  1:255764/1‑148 (MQ=255)                                                                              aTTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCaa                                                                  <  6:44413/149‑1 (MQ=255)                                                                                  gggTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATtgt                                                              <  8:107564/149‑1 (MQ=255)                                                                                     tGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGc                                                                          >  3:185208/1‑134 (MQ=255)                                                                                     tGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGc                                                                          <  4:185208/134‑1 (MQ=255)                                                                                       cAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGt                                                          >  7:269060/1‑148 (MQ=255)                                                                                         aCCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCaa                                                       <  2:158886/149‑1 (MQ=255)                                                                                            tGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCTTACGGGAAATTGTGCCTgt                                                                      >  2:357128/1‑130 (MQ=255)                                                                                              tATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTc                                                   >  3:308890/1‑148 (MQ=255)                                                                                              tATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTCGCGTACGGGAAATTTGGCCTGGTCAATTGTTCGTCAACTTc                                                   >  6:91685/1‑148 (MQ=255)                                                                                                tCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCaa                                                 <  4:276931/148‑1 (MQ=255)                                                                                                  acacCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAatat                                              <  2:317323/149‑1 (MQ=255)                                                                                                    acCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAatat                                              >  5:105866/1‑147 (MQ=255)                                                                                                      ccGATGAAAGTTTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGc                                          <  5:91685/149‑1 (MQ=255)                                                                                                      ccGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTg                                                                       >  5:293060/1‑120 (MQ=255)                                                                                                      ccGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTg                                                                       <  6:293060/120‑1 (MQ=255)                                                                                                       cGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGc                                          >  7:66811/1‑148 (MQ=255)                                                                                                        gATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGCTGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCaa                                                 <  7:312154/140‑1 (MQ=255)                                                                                                        gATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGTAAAGTGGGCCTGGTCAATTGTGCGTCAAGTTCaa                                                 >  8:312154/1‑140 (MQ=255)                                                                                                        gATGAAAGATTAATAAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGTCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGGCAACTTCAATATGAGCaa                                        <  5:171374/149‑1 (MQ=255)                                                                                                          tGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTc                                                   <  6:44819/136‑1 (MQ=255)                                                                                                          tGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTc                                                   >  5:44819/1‑136 (MQ=255)                                                                                                          tGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACg                                      >  2:206168/1‑149 (MQ=255)                                                                                                          agAAAGATTAAAAATTCAATATTATGATATCTATTTAAAGTATAATCCGTATAATCTTACTGCCTCAGGCTTGCTTTTAAGCGATGATCAGACTGGCGTAACGGAAAATTCGACTGGTCAATTGTACCACAACTTCAATATGAGCAAc                                       >  4:104935/2‑148 (MQ=255)                                                                                                            aaaGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGc                                     >  3:235637/1‑148 (MQ=255)                                                                                                              aGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTAt                                  >  8:259259/1‑149 (MQ=255)                                                                                                              aGATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTAt                                  <  7:259259/149‑1 (MQ=255)                                                                                                               gATTAATTAGTCAAGATTATTATATCTTTTTAACGTATAATCCGTATAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATTATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATg                                 <  2:266267/149‑1 (MQ=255)                                                                                                               gATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTTCTGCctc                                                                                                                         <  8:310606/61‑1 (MQ=255)                                                                                                               gATTAATTAGTCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTTCTGCctc                                                                                                                         >  7:310606/1‑61 (MQ=255)                                                                                                                 ttaattaGTCAAGATTATGATATCTTTATAACGTATAATCCGTCTTATCTTACTGCCTCTGGCTTGCTTTTAAGCgatg                                                                                                     >  1:341183/1‑79 (MQ=255)                                                                                                                         tCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGa                                            <  7:162025/128‑1 (MQ=255)                                                                                                                         tCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGa                                            <  7:156159/128‑1 (MQ=255)                                                                                                                         tCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGa                                            >  8:156159/1‑128 (MQ=255)                                                                                                                         tCAAGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGa                                            >  8:162025/1‑128 (MQ=255)                                                                                                                           aaGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTc                     >  2:130017/1‑149 (MQ=255)                                                                                                                           aaGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTc                     >  7:57605/1‑149 (MQ=255)                                                                                                                           aaGATTATGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTc                     >  2:54195/1‑149 (MQ=255)                                                                                                                                 aTGATATCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACa                <  2:294878/148‑1 (MQ=255)                                                                                                                                    atatCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACaatta            <  8:66811/149‑1 (MQ=255)                                                                                                                                    atatCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACaatt             <  4:235637/148‑1 (MQ=255)                                                                                                                                       tCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACaattaatt         >  2:302060/1‑149 (MQ=255)                                                                                                                                       tCTTTTTAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGACTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACaattaatt         >  1:266256/1‑149 (MQ=255)                                                                                                                                         tttttAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTg        >  6:221136/1‑148 (MQ=255)                                                                                                                                           tttAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTg        <  1:206168/146‑1 (MQ=255)                                                                                                                                           tttAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGaa      <  1:95743/148‑1 (MQ=255)                                                                                                                                           tttAACGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGaa      <  1:128803/148‑1 (MQ=255)                                                                                                                                            ttAACTTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAg     <  3:104935/148‑1 (MQ=255)                                                                                                                                               aCGTATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGc  >  4:270560/1‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                                 TTTACTGGATGATGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGC  >  NC_000913/2315554‑2315843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑