| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,596,873 | T→A | 6.8% | pseudogene (1115/3861 nt) | yneO ← | pseudogene, AidA homolog |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,596,873 | 0 | T | A | 6.8% | 156.6 / 2.4 | 59 | pseudogene (1115/3861 nt) | yneO | pseudogene, AidA homolog |
| Reads supporting (aligned to +/- strand): ref base T (34/21); new base A (2/2); total (36/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01 | |||||||||||
TGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTCGAGACATTAAGTTCACCGTC > NC_000913/1596733‑1597021 | tggtggTATAGACATTCTTATTGACCTCCCACATTCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCa < 3:124643/149‑1 (MQ=255) tggtATAGACATTCTTATTGACCTCCCACATTCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATaa < 8:350184/149‑1 (MQ=255) ataGACATTCTTATTGACCTCCCACATTCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGCACTTCCTCCGTTGCTGACAACACGCCCCTGATTACTGATATTCATTTCGCCGAGGCCGTCATaaac > 1:226915/1‑145 (MQ=255) ataGACATTCTTATTGACCTCCCACATTCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGAGGCCGTCATAAcc > 1:220959/1‑147 (MQ=255) aGACATTCTTATTGACCTCCCACATTCCCCCCGTGGTGATGATGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGATCTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAAcc > 5:380239/1‑145 (MQ=255) aCATTCTTATTGACCTCCCACATTCACCCCGTGGTGATGCTGACATTCCCGAAGCCTGTTTCTCAATAACCGAGTGAACTTCCTCCGTTGATGAAAACCAGAACGTGATTACTCAGATTCATTTCTCAGTGGCCGTCATAACCAAGAt > 5:156544/1‑148 (MQ=255) aTTCTTATTGACCTCCCACATTCCCCCCGTGGTGATGCTGACATTCCCGCCGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAg > 7:95214/1‑149 (MQ=255) cttGACCTCCCACATTCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTcc > 7:276829/2‑147 (MQ=255) aTTGACCTCCCACATTCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTcc > 6:169612/1‑147 (MQ=255) aTTGACCTCCCACATTCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTcc < 5:169612/147‑1 (MQ=255) tCCCACATTCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGt > 4:295851/1‑149 (MQ=255) ccACATTCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGt < 7:195889/147‑1 (MQ=255) caTTCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTATCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATaa > 6:71652/1‑149 (MQ=255) tCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGAt > 1:282946/1‑125 (MQ=255) tCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGAt < 2:282946/125‑1 (MQ=255) cccccGTGGTGATGCTGACATTCCCGACGCCGGTTTGTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGa < 5:71652/148‑1 (MQ=255) cTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTaa > 3:251250/1‑148 (MQ=255) tGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCATTaa > 7:167519/1‑147 (MQ=255) gACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAg > 6:14171/1‑149 (MQ=255) aCATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGt < 3:295851/149‑1 (MQ=255) cATTCCAGACGCCGGTTTCTACATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTGGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTa < 6:380239/149‑1 (MQ=255) tacccGAAGACTGTGTCTCCATATCAGAGTGAACTTCCTCATTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTTGCCTTCATACCCAAGATTAGTTCAGAGATTAGTGATAACGGAGTTCTTATGCTTACCAGTAAt < 6:156544/147‑1 (MQ=255) gACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTtgc > 7:136815/1‑149 (MQ=255) ccGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGAGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTtgcctgt < 2:220959/149‑1 (MQ=255) ccGGTTTCTACATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGAGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTtgcctgt < 2:226915/149‑1 (MQ=255) cGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTtgcctgtg > 3:211297/1‑149 (MQ=255) gTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCtt < 3:21355/122‑1 (MQ=255) gTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCtt > 4:21355/1‑122 (MQ=255) tttCTCCATAACCGAGTGAACTTCCTCCGTTGCTGCCAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTtgcctgtgcct > 1:291029/1‑149 (MQ=255) tcCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGttt > 1:141345/1‑149 (MQ=255) ccataCCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGtttct > 5:208954/5‑149 (MQ=255) cATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGttt < 3:128794/147‑1 (MQ=255) aTAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGtttctt < 4:251250/149‑1 (MQ=255) aCCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCa > 2:326771/1‑149 (MQ=255) cGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATc > 5:166411/1‑149 (MQ=255) gAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCg > 2:339600/1‑148 (MQ=255) tccGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATaa > 6:339107/1‑149 (MQ=255) ccGTTGCTGACAACAAGCCCCTGATTACCGATATTCATTTCGCCGTGGCCGTCATAAACAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAAt > 5:79153/1‑149 (MQ=255) gCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTccc < 2:291029/148‑1 (MQ=255) gACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAAt > 7:139949/1‑149 (MQ=255) acaacaAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCAGaaac > 5:310736/1‑147 (MQ=255) aacaaGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTa > 3:115861/1‑149 (MQ=255) aacaaGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTa > 8:86862/1‑149 (MQ=255) ccTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCt < 6:208954/149‑1 (MQ=255) aTTACTGATATTCATTTCGCAGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCa < 2:67924/148‑1 (MQ=255) aCTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTc < 7:86862/148‑1 (MQ=255) tGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTc > 1:226850/1‑146 (MQ=255) gATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAAAAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGt > 8:48565/1‑114 (MQ=255) gATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAAAAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGt < 7:48565/114‑1 (MQ=255) atatTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTCgaga < 5:14171/148‑1 (MQ=255) atTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGcc < 5:338543/135‑1 (MQ=255) atTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGcc > 6:338543/1‑135 (MQ=255) ttCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTCGAGACAt < 4:211297/148‑1 (MQ=255) ttCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTCGAGACAt > 7:338409/1‑148 (MQ=255) ttCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTCGAGACAt < 2:226850/148‑1 (MQ=255) ttCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTACCGAATCTACTTTGCCTTCACTCGAGACATTAAGtt > 6:27764/1‑149 (MQ=255) ccGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTCGAGACATTAAGTTCAcc > 3:324197/1‑149 (MQ=255) ccGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTCGAGACATTAAGTTCAcc > 7:104577/1‑149 (MQ=255) ccGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTCGAGACATTAAGTTCAcc > 6:282447/1‑149 (MQ=255) tGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTCGAGACATTAAGTTCACCGTc < 6:166411/149‑1 (MQ=255) tGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTCGAGACATTAAGTTCACCGTc < 8:338409/149‑1 (MQ=255) | TGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCCCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATTACTGATATTCATTTCGCCGTGGCCGTCATAACCAAGATTAGTTCCGAGATTAGTGATAACGGAGTTCTTATCCTTAACAGTAATGTTGCCTGTGCCTGTTTCTTTCATCCCCGCAGTGATAATTCCCGAATCTACTTTGCCTTCACTCGAGACATTAAGTTCACCGTC > NC_000913/1596733‑1597021 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |