| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,252,810 | T→G | 14.3% | intergenic (+22/+85) | yeiI → / ← nupX | putative kinase/nucleoside permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,252,810 | 0 | T | G | 14.3% | 74.9 / 5.4 | 35 | intergenic (+22/+85) | yeiI/nupX | putative kinase/nucleoside permease |
| Reads supporting (aligned to +/- strand): ref base T (18/12); new base G (2/3); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
TACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCG > NC_000913/2252666‑2252953 | tACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAAc > 3:2372/1‑149 (MQ=255) cgACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATcc < 4:2372/149‑1 (MQ=255) gACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATAAGACGCGTTAAGCGTCGCATCCg > 2:315750/1‑149 (MQ=255) cAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGc < 3:264221/149‑1 (MQ=255) aGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATAAGACGCGTTAAGCGTCGCATCCGGca < 1:315750/149‑2 (MQ=255) gcgcCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATAAGACGCGTTAAGCGTCGCATCCGGcaa < 8:273227/149‑3 (MQ=38) cccGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTAc > 7:362665/1‑148 (MQ=255) ccGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACa > 1:189150/1‑148 (MQ=255) cgcgGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGc < 8:122619/147‑1 (MQ=255) ggCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGt > 8:85729/1‑148 (MQ=255) ttCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATg > 8:303864/1‑147 (MQ=255) cgcgcgCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAg < 8:362665/148‑1 (MQ=255) gCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTgcg < 7:303864/149‑1 (MQ=255) ttAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCa > 6:38034/1‑149 (MQ=255) aacaacCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTTACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATc < 4:141435/148‑1 (MQ=255) aacCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCt > 6:329292/1‑137 (MQ=255) aacCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCt < 5:329292/137‑1 (MQ=255) aacCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAgg > 3:78771/1‑148 (MQ=255) aacCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAgg > 5:311955/1‑148 (MQ=255) ctaccctGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCaa > 4:61580/1‑111 (MQ=255) ctaccctGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCaa < 3:61580/111‑1 (MQ=255) ccctGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGaca > 5:40991/1‑149 (MQ=255) tGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGc > 4:138539/1‑149 (MQ=255) cgcgCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAAt < 2:189150/149‑1 (MQ=255) ccAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCa < 2:292573/111‑1 (MQ=255) aTGGAGTAACAATGTTCACCGATGCTACGCTTAACTCCTCTTATACCGCCTACAAATCAGCCAAATACAGCAAGAAAATCAACGCATGATAAGCTTACTCATCAGGCAATGACACTCAAGCTAAACCAATAAAGACACCGGAAATggac > 4:146836/1‑147 (MQ=255) tgtAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCAc < 2:37338/149‑1 (MQ=255) aaTGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCATGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGCCACTCAAGCGAAACCAATTAAGCACCCGGCAATGGTCGCACtcatca > 7:348591/1‑149 (MQ=39) ccGGATAAGACGCGTTAAGCGTCGCATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTgg > 3:88695/1‑148 (MQ=39) gATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAg > 4:370291/1‑149 (MQ=255) gATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAg > 1:19565/1‑149 (MQ=255) aTGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGc < 6:40991/149‑1 (MQ=255) gCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGt > 4:165443/1‑149 (MQ=255) agACGCGTTAAGCGTCGCATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTc < 4:88695/148‑1 (MQ=38) gACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTcg > 6:20738/1‑149 (MQ=255) | TACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCG > NC_000913/2252666‑2252953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |