Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 566,728 G→T 100% pseudogene (45/93 nt) renD → pseudogene, DLP12 prophage;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913566,7280GT98.4% 6750.2 / 33.2 2308pseudogene (45/93 nt)renDpseudogene, DLP12 prophage;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base G (20/23);  new base T (1234/1023);  total (1261/1047)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 2317 total aligned reads displayed.

AAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGCGCAACCGTAATCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCG  >  NC_000913/566582‑566876
                                                                                                                                                  |                                                                                                                                                    
  accaacTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGATATTATTCATTACCTGGGGACGCATAATAGCt                                                                                                                                                  <  2:109174/147‑1 (MQ=255)
  aacaacTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCt                                                                                                                                                  >  2:334917/1‑149 (MQ=255)
  aacaacTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCt                                                                                                                                                  >  4:164842/1‑149 (MQ=255)
  aacaacTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCt                                                                                                                                                  >  7:170188/1‑149 (MQ=255)
  aaaaacTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGCCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACCGGCAAAGAGGCAATTATTCATTACCGGGGGACGCATAATAGCt                                                                                                                                                  >  1:235950/4‑149 (MQ=255)
   acaacTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCTCAGAAATCAAAGCGAAGTTCGGACTGAAAGGAGCAAGTGTATGCCGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCtt                                                                                                                                                 <  3:189899/149‑1 (MQ=255)
   acaacTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCtt                                                                                                                                                 <  4:49857/149‑1 (MQ=255)
   acaacTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCtt                                                                                                                                                 >  6:144546/1‑149 (MQ=255)
    caacTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGTACGCATAATAGCTTc                                                                                                                                                >  1:17607/1‑149 (MQ=255)
    caacTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTc                                                                                                                                                <  6:173778/149‑1 (MQ=255)
       cTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCtgt                                                                                                                                             <  7:39480/149‑1 (MQ=255)
       cTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCtgt                                                                                                                                             >  8:65284/1‑149 (MQ=255)
              tcaTGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGcgccg                                                                                                                                       >  8:134464/3‑144 (MQ=255)
              tcaTGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGcgccg                                                                                                                                       >  2:57965/3‑144 (MQ=255)
                aTGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGa                                                                                                                                     <  5:241133/148‑1 (MQ=255)
                aTGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGAc                                                                                                                                    >  3:165605/1‑149 (MQ=255)
                aTGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGATCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGAc                                                                                                                                    >  3:107641/1‑149 (MQ=255)
                 tGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACg                                                                                                                                   >  3:317370/1‑149 (MQ=255)
                    gCGGGAGACCGCTAAATCGTTCACAGGCTCTGGCGAAGATCGCAGAATTCAAAGCTAAGTTCGGACTGAAAGGAGCAATTGAATGACCGGCAAAGAGGCAATTATGCATTAACTTGGCCCGCATAATCCCCTCTGAGCGCCGGACGTTg                                                                                                                                <  4:355211/149‑1 (MQ=255)
                     cGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGtt                                                                                                                                 <  3:69753/147‑1 (MQ=255)
                        tAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCcgc                                                                                                                            <  1:134341/149‑1 (MQ=255)
                        tAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCAAAATAGCTTCTGTGCGCCGTCCGTTGCcgc                                                                                                                            >  7:353551/1‑149 (MQ=255)
                          gACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCcgcgc                                                                                                                          <  2:237583/149‑2 (MQ=255)
                             ctctAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTaa                                                                                                                       <  8:297225/149‑1 (MQ=255)
                             ctctAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTaa                                                                                                                       >  1:26587/1‑149 (MQ=255)
                              tctAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAAc                                                                                                                      >  3:219993/1‑149 (MQ=255)
                              tctAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTaa                                                                                                                       >  8:265656/1‑148 (MQ=255)
                              tctAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTaa                                                                                                                       >  3:340508/1‑148 (MQ=255)
                                tAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCTGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAAc                                                                                                                      <  4:230824/147‑1 (MQ=255)
                                tAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAg                                                                                                                    >  3:9269/1‑149 (MQ=255)
                                  aaTCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACa                                                                                                                     <  1:87223/146‑1 (MQ=255)
                                  aaTCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGgc                                                                                                                  <  4:201877/149‑1 (MQ=255)
                                  aaTCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGgc                                                                                                                  <  7:295279/149‑1 (MQ=255)
                                  aaTCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGgc                                                                                                                  <  7:246556/149‑1 (MQ=255)
                                  aaTCGTGCACAGGCTCTGGCGAAGATCGCAGAAAGCAAAGATAAGTTCGGTCTGATAGGAGCAAGTGTATGCCGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGgc                                                                                                                  <  3:256004/149‑1 (MQ=255)
                                       tGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCaa                                                                                                              >  1:102130/1‑148 (MQ=255)
                                         cacaGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAAca                                                                                                            <  6:346019/148‑2 (MQ=255)
                                          acaGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAAcag                                                                                                           >  1:386898/1‑146 (MQ=255)
                                          acaGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGt                                                                                                          <  6:9104/149‑1 (MQ=255)
                                          acaGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGACAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGt                                                                                                          <  2:387192/149‑1 (MQ=255)
                                             ggCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGTCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGATTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAc                                                                                                       <  6:376110‑M1/149‑2 (MQ=255)
                                                  tGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagca                                                                                                  >  5:48745‑M1/1‑143 (MQ=255)
                                                  tGGAGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGAACCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagca                                                                                                  >  8:398488‑M1/1‑143 (MQ=255)
                                                    gCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGATGCATAATAGCTTCt                                                                                                                                               >  8:235712/1‑102 (MQ=255)
                                                    gCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcat                                                                                                 <  7:314079‑M1/148‑8 (MQ=255)
                                                    gCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcat                                                                                                 >  7:68562‑M1/1‑141 (MQ=255)
                                                      gAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTACCAGGCGCAACAGTAAccagcataaa                                                                                              >  8:101060‑M1/1‑139 (MQ=255)
                                                      gAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaa                                                                                              >  3:282477‑M1/1‑139 (MQ=255)
                                                      gAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaa                                                                                              >  7:273761‑M1/1‑139 (MQ=255)
                                                       aaGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaat                                                                                             >  7:373180‑M1/1‑138 (MQ=255)
                                                        aGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGtt                                                                                                                                 <  4:333699/112‑1 (MQ=255)
                                                         gATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaat                                                                                             >  5:311935‑M1/1‑136 (MQ=255)
                                                         gATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAc                                                                                                       >  7:141671‑M1/1‑136 (MQ=255)
                                                           tCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcag                                                                                          <  8:185601‑M1/148‑15 (MQ=255)
                                                             gCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggcc                                                                                       >  2:143401‑M1/1‑132 (MQ=255)
                                                             gCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggcc                                                                                       >  1:31732‑M1/1‑132 (MQ=255)
                                                                    tcaaagcTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggcta                                                                                <  6:205745‑M1/147‑25 (MQ=255)
                                                                      aaaGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaa                                                                               <  1:201775‑M1/148‑26 (MQ=255)
                                                                        aGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaat                                                                            >  5:98198‑M1/1‑121 (MQ=255)
                                                                        aGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaat                                                                            <  4:78476‑M1/149‑29 (MQ=255)
                                                                         gCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatg                                                                           >  8:161235‑M1/1‑120 (MQ=255)
                                                                          cTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcag                                                                                          >  5:101110‑M1/1‑119 (MQ=255)
                                                                           tAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatgg                                                                          >  3:366279‑M1/1‑118 (MQ=255)
                                                                             aGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcac                                                                       <  4:141157‑M1/149‑34 (MQ=255)
                                                                              gTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacg                                                                      >  5:63817‑M1/1‑115 (MQ=255)
                                                                               ttCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgg                                                                     >  7:11972‑M1/1‑114 (MQ=255)
                                                                                  ggACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataactccggccgcggctaaaatggcacggg                                                                    >  2:302655‑M1/1‑111 (MQ=255)
                                                                                  ggACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggc                                                                   <  2:364896‑M1/148‑38 (MQ=255)
                                                                                   gACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcag                                                                 >  6:233532‑M1/1‑110 (MQ=255)
                                                                                       gAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtct                                                             >  2:213540‑M1/1‑106 (MQ=255)
                                                                                        aaaGGAGCAAGTGTATGACGGGCAAAGAGGCTATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtctt                                                            >  4:3072‑M1/1‑105 (MQ=255)
                                                                                        aaaGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtctt                                                            >  6:320121‑M1/1‑105 (MQ=255)
                                                                                        aaaGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtctt                                                            >  8:374447‑M1/1‑105 (MQ=255)
                                                                                        aaaGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtctt                                                            >  7:198041‑M1/1‑105 (MQ=255)
                                                                                        aaaGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtctt                                                            <  8:320406‑M1/149‑45 (MQ=255)
                                                                                        aaaGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtctt                                                            >  2:18897‑M1/1‑105 (MQ=255)
                                                                                        aaaGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaa                                                                             >  6:116349‑M1/1‑105 (MQ=255)
                                                                                        aaaGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAcca                                                                                                     <  3:141387‑M1/108‑4 (MQ=255)
                                                                                        aaaGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGCGCAACCGTAATCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTa                                                             >  5:150489/1‑148 (MQ=255)
                                                                                        aaaGGAGCAAGTGTATGACGGGCAAAGAGGCAATGATTCATTACATGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcccgggcaggactt                                                            >  5:215186‑M1/1‑105 (MQ=255)
                                                                                         aaGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACACTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttc                                                           >  6:61865‑M1/1‑104 (MQ=255)
                                                                                         aaGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTAATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGATAAGAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtctt                                                            <  6:291881‑M1/148‑45 (MQ=255)
                                                                                              gCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggt                                                       >  6:271953‑M1/1‑99 (MQ=255)
                                                                                              gCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggt                                                       >  8:350143‑M1/1‑99 (MQ=255)
                                                                                               cAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggtta                                                     >  1:291756‑M1/1‑98 (MQ=255)
                                                                                                 aGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAcgagcataaatcaggccgcggctaaaatggcacgggcaggtcttct                                                          <  5:279559‑M1/142‑47 (MQ=255)
                                                                                                 aGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcagggcttctggttatc                                                   <  5:117273‑M1/149‑54 (MQ=255)
                                                                                                  gtgtATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatcgcacgggcaggtcttctggttatc                                                   >  4:109768‑M1/1‑95 (MQ=255)
                                                                                                   tgtATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcg                                                  >  1:150835‑M1/1‑94 (MQ=255)
                                                                                                   tgtATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcg                                                  >  1:35520‑M1/1‑94 (MQ=255)
                                                                                                     tATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaa                                                <  2:163871‑M1/148‑57 (MQ=255)
                                                                                                     tATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaa                                                <  8:214567‑M1/148‑57 (MQ=255)
                                                                                                         aCGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaa                                           <  4:263174‑M1/149‑62 (MQ=255)
                                                                                                         aCGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaa                                           <  2:26481‑M1/149‑62 (MQ=255)
                                                                                                             gCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAc                                                                                                       <  7:225027‑M1/85‑2 (MQ=255)
                                                                                                                aaGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctgg                                    <  1:53047‑M1/149‑69 (MQ=255)
                                                                                                                       aaTTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCGCCGGa                                                                                                                                     >  5:368666/1‑45 (MQ=255)
                                                                                                                                   ccTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaacggtgtattaccggt                 <  5:338376‑M1/149‑88 (MQ=255)
                                                                                                                                      ggggACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaacggtgtattaccggtttg              >  5:186095‑M1/1‑59 (MQ=255)
                                                                                                                                      ggggACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaacggtgtattaccggtttg              >  2:86626‑M1/1‑59 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGCGCAACCGTAATCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCG  >  NC_000913/566582‑566876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: