Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,034,283 A→T 5.2% Y380F (TAT→TTT)  trkH → potassium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,034,2830AT 5.2% 209.6 / 2.0 77Y380F (TAT→TTT) trkHpotassium transporter
Reads supporting (aligned to +/- strand):  ref base A (35/38);  new base T (2/2);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

CTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTC  >  NC_000913/4034135‑4034427
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ctctTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGGGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCta                                                                                                                                                   <  5:263848/149‑1 (MQ=255)
   tttttGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAg                                                                                                                                                >  7:341865/1‑149 (MQ=255)
     tttGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAg                                                                                                                                                <  4:214127/147‑1 (MQ=255)
     tttGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAg                                                                                                                                                <  1:265436/147‑1 (MQ=255)
               cTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTggg                                                                                                                                    <  3:72837/149‑1 (MQ=255)
               cTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTggg                                                                                                                                    <  5:8677/149‑1 (MQ=255)
                tGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTggg                                                                                                                                    >  5:279716/1‑148 (MQ=255)
                 gCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTgggg                                                                                                                                   >  1:4401/1‑148 (MQ=255)
                  cTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGaa                                                                                                                                 >  7:41966/1‑149 (MQ=255)
                   ttttATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCAtt                                                                                                                                            >  6:350165/1‑137 (MQ=255)
                   ttttATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCAtt                                                                                                                                            <  5:350165/137‑1 (MQ=255)
                   ttttATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAAt                                                                                                                                >  7:17183/1‑149 (MQ=255)
                     ttATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATc                                                                                                                               <  6:279716/148‑1 (MQ=255)
                      tATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATcgc                                                                                                                             >  7:183028/1‑149 (MQ=255)
                          ttCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGGGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCa                                                                                                                          <  2:110515/148‑1 (MQ=255)
                            cAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTg                                                                                                                       >  2:257682/1‑149 (MQ=255)
                               ccTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCg                                                                                                                    >  5:153188/1‑149 (MQ=255)
                                cTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCg                                                                                                                    >  4:256123/1‑148 (MQ=255)
                                 tttATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCgg                                                                                                                   >  6:313225/1‑148 (MQ=255)
                                  ttATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGaa                                                                                                                 >  8:156045/1‑149 (MQ=255)
                                    aTCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAAc                                                                                                                <  7:180738/148‑1 (MQ=255)
                                              gcgcCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGaa                                                                                                     >  6:78200/1‑149 (MQ=255)
                                                     gTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGttt                                                                                              <  2:4401/149‑1 (MQ=255)
                                                     gTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGttt                                                                                              <  5:78019/149‑1 (MQ=255)
                                                            ggCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑tt                                                                                      <  7:36926/149‑1 (MQ=255)
                                                             gcggcgGCCTGAAGGGGCTCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCAGCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑ttt                                                                                     <  8:121537/149‑1 (MQ=255)
                                                                   gCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGATGCCGTTTGGGGA‑TTTTTCTcc                                                                               <  8:41966/149‑1 (MQ=255)
                                                                   gCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTcc                                                                               <  8:17183/149‑1 (MQ=255)
                                                                   gCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTcc                                                                               <  6:153188/149‑1 (MQ=255)
                                                                   gCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTcc                                                                               <  8:183028/149‑1 (MQ=255)
                                                                      tGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGcc                                                                            >  7:319234/1‑149 (MQ=255)
                                                                          ggTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTTTAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATg                                                                        >  8:43616/1‑149 (MQ=255)
                                                                          ggTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTTTAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATg                                                                        >  8:37577/1‑149 (MQ=255)
                                                                              aTCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGa                                                                                         >  3:84490/1‑129 (MQ=255)
                                                                              aTCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGa                                                                                         <  4:84490/129‑1 (MQ=255)
                                                                              aTCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCAtt                                                                    <  8:341865/149‑1 (MQ=255)
                                                                              aTCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCAtt                                                                    >  5:375825/1‑149 (MQ=255)
                                                                                 cGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGCCTGGTGCATCCGAACGCCGTCGATAGCATTAAGCTGGGGACTCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑GTTTTGTCCGCCTATGCATTGgt                                                                 >  5:374176/1‑149 (MQ=255)
                                                                                  gCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGgtg                                                                <  7:85526/149‑1 (MQ=255)
                                                                                   cATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGttt                                                                                              <  8:206769/119‑1 (MQ=255)
                                                                                   cATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGttt                                                                                              >  7:206769/1‑119 (MQ=255)
                                                                                     tCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGtt                                                              >  7:20909/1‑148 (MQ=255)
                                                                                      ccTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGATTCGCGCACTGCCGGAACGTCTCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTa                                                            >  6:40515/1‑149 (MQ=255)
                                                                                          ctgctgTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTAtt                                                          <  5:313225/147‑1 (MQ=255)
                                                                                          ctgctgTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGt                                                        <  7:156045/149‑1 (MQ=255)
                                                                                             ctgTTTAAGCAGGGGAACCTTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATATCATTAAGCTTGGGAATCGCGCAGTGCCGGAACGTATCATCGAAGCCGTTTTGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCa                                                      <  1:245974/148‑1 (MQ=255)
                                                                                             ctgTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCTAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCa                                                      <  1:248343/148‑1 (MQ=255)
                                                                                             ctgTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGttt                                                                                              <  1:391513/109‑1 (MQ=255)
                                                                                             ctgTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGttt                                                                                              >  2:391513/1‑109 (MQ=255)
                                                                                             aggtgtAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGGCGTCTATACGAATAAGCTGGGGAATCGCGCACTGCCGGCACGTATCCTCGACCCCGTTGGGGGA‑TTTTTCTCCGCCTATGCATTCGTGGTTAAGGTCAg                                                     >  8:46214/6‑149 (MQ=255)
                                                                                               gTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTa                                                   >  7:329348/1‑149 (MQ=255)
                                                                                                tttAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTa                                                   <  8:20909/148‑1 (MQ=255)
                                                                                                tttAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTAt                                                  >  3:155358/1‑149 (MQ=255)
                                                                                                           gAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTa                                       >  6:100772/1‑149 (MQ=255)
                                                                                                           gAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTa                                       >  5:112215/1‑149 (MQ=255)
                                                                                                             aCCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAt                                      <  5:78200/148‑1 (MQ=255)
                                                                                                             aCCGTGAGCTGAAACGACTGGTGCATCCGAACACCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATc                                     <  1:215713/149‑1 (MQ=255)
                                                                                                                   aGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCa                                 <  3:183139/148‑1 (MQ=255)
                                                                                                                      tGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGTAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCACTATGCTGGCGATTATCGCCACGGGc                            >  2:132153/1‑149 (MQ=255)
                                                                                                                      tGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGc                            >  3:5568/1‑149 (MQ=255)
                                                                                                                      tGAAACGACTGGTGCATCCCAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGc                            >  4:116412/1‑149 (MQ=255)
                                                                                                                       gAACCGACTGGTGCATCCGACCGCCGTCTATAGCATTACGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGGTTGGGGA‑TTTTTCTCCGCCTCTGCAGTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCg                           >  6:287437/1‑149 (MQ=255)
                                                                                                                       gAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCg                           >  1:191442/1‑149 (MQ=255)
                                                                                                                        aaaCGACTGGTGCATCCGAACGCCGTCTTTAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGt                          <  7:43616/149‑1 (MQ=255)
                                                                                                                        aaaCGACTGGTGCATCCGAACGCCGTCTTTAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGt                          <  7:37577/149‑1 (MQ=255)
                                                                                                                           cGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGa                       <  8:319234/149‑1 (MQ=255)
                                                                                                                               tGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑‑TTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGc                                   >  7:298974/1‑132 (MQ=255)
                                                                                                                               tGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑‑TTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGc                                   <  8:298974/132‑1 (MQ=255)
                                                                                                                                     atacGAACGCCGTCTATAGCATTAAGCTGGGGAATCTCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGCTGTTTATTTTCAGTATGCTGGCTCTTATCGCCACGGGCGTGGATGACTTTTCt             <  1:303123/146‑1 (MQ=255)
                                                                                                                                     aTCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATAACTTTTCt             <  4:5568/149‑1 (MQ=255)
                                                                                                                                      tCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGa                       >  2:63583/1‑138 (MQ=255)
                                                                                                                                      tCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGa                       <  1:63583/138‑1 (MQ=255)
                                                                                                                                         gAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCt         >  5:29690/1‑149 (MQ=255)
                                                                                                                                          aaCGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCtt        >  4:303052/1‑149 (MQ=255)
                                                                                                                                           aCGCCGTCTATAGCATTAAGCTGGGGAAGCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCttt       <  3:51635/149‑1 (MQ=255)
                                                                                                                                             gCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGc     <  3:303052/149‑1 (MQ=255)
                                                                                                                                                gTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTc  <  3:256123/149‑1 (MQ=255)
                                                                                                                                                gTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTc  <  1:257682/149‑1 (MQ=255)
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CTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGA‑TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTC  >  NC_000913/4034135‑4034427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑