Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,190,208 T→A 5.7% R141R (CGT→CGA yjaZ → stationary phase growth adaptation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,190,2080TA 5.7% 228.0 / 2.5 86R141R (CGT→CGAyjaZstationary phase growth adaptation protein
Reads supporting (aligned to +/- strand):  ref base T (42/37);  new base A (2/3);  total (44/42)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

AAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAACCCACCTTAAGGTGGGTTTCGCCAGA  >  NC_000913/4190062‑4190356
                                                                                                                                                  |                                                                                                                                                    
aaaaGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGttt                                                                                                                                                    <  3:52055/149‑1 (MQ=255)
  aaGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGc                                                                                                                                                  <  8:3565/149‑1 (MQ=255)
   aGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTAACTTTGTTGCGTTCAGGGCTTTCAGGTAAAAGAAAAAACTCCGTTTGCa                                                                                                                                                 >  2:366812/1‑149 (MQ=255)
       caaTTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGt                                                                                                                                              >  7:46051/3‑148 (MQ=255)
            ttGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTa                                                                                                                                         <  1:138286/148‑1 (MQ=255)
                     ccAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATcc                                                                                                                               >  6:275548/1‑149 (MQ=255)
                      cAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATcc                                                                                                                               >  6:142276/1‑148 (MQ=255)
                           aGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTc                                                                                                                         >  7:156971/1‑149 (MQ=255)
                              aaCGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTg                                                                                                                       >  2:214136/1‑148 (MQ=255)
                               aCGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAgc                                                                                                                                       >  8:185801/1‑131 (MQ=255)
                               aCGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAgc                                                                                                                                       <  7:185801/131‑1 (MQ=255)
                                        aTGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGaaa                                                                                                            <  1:56168/149‑1 (MQ=255)
                                        aTGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGaaa                                                                                                            <  5:142276/149‑1 (MQ=255)
                                        aTGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGaa                                                                                                             <  3:297445/148‑1 (MQ=255)
                                        aTGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGa                                                                                                              >  8:84959/1‑147 (MQ=255)
                                        aTGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGa                                                                                                              >  7:367658/1‑147 (MQ=255)
                                         tGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGaaa                                                                                                            <  8:345279/148‑1 (MQ=255)
                                         tGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGa                                                                                                              >  7:345279/1‑146 (MQ=255)
                                         tGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAg                                                                                                           >  3:237981/1‑149 (MQ=255)
                                         tGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAg                                                                                                           <  7:37398/149‑1 (MQ=255)
                                          gCCGGGTATGGTAGCTATGCATTGATTTAGGTGGACTTAGACGGAGGAGATAATGCATTTTACTTGGTTTCGTTCATGTCTGTCAGGGAAAAGAAAAAACTCCGTTTGCAGGTAACTAGCGCTTATCCAATATATGAAACACCGaaatg                                                                                                          >  6:300472/1‑147 (MQ=255)
                                           ccGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGt                                                                                                         <  6:17470/149‑1 (MQ=255)
                                           ccGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGt                                                                                                         <  4:11945/149‑1 (MQ=255)
                                                             ccGTGATTGAGGTGGACTTAGACGGAGGAGATAATGCATTTTACTTTGTTGCGTTCAGGGCTTTCATGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAAttttt                                                                                        <  3:192073/148‑1 (MQ=255)
                                                             ccGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTc                                                                                       <  3:324696/149‑1 (MQ=255)
                                                              cGTGCTTGAGGGGGACTTAGACGTAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCa                                                                                      <  8:291518/149‑1 (MQ=255)
                                                               gTGATTGAGGTGGACTTAGACGGAGGAGATAAGGGATTTTACTTTGTTGCGTTCAGGGGTTTCAGGGAAAAGTAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCTTTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCAc                                                                                     <  6:32299/149‑1 (MQ=255)
                                                                 gATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCa                                                                                   >  8:76682/1‑149 (MQ=255)
                                                                  aTTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAAATCCGTTTTCATGTAACTAGCTCTTATCCCATTTCTGAAAAACAGAAAGGTAAAACATGGAAAGTTTTCAACAt                                                                                  >  6:289726/1‑149 (MQ=255)
                                                                      aGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGcc                                                                              <  5:275548/149‑1 (MQ=255)
                                                                         tGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCAATTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTAc                                                                           <  1:370312/149‑1 (MQ=255)
                                                                              ttAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACt                                                                       >  3:85395/1‑148 (MQ=255)
                                                                                aGACGGAGGAGATAACTCATTTTACTTTGTTGCCATCAGGGCTTGCCGGGAACAGAAAAAACTGCGTTTGCATGTAACTAGCGCCTATCCCATTTCTGAAAACCCGAAAGGTAAATCAGCGAAATTTTTCACAATTGCCTACTACTTa                                                                     <  6:260736/148‑1 (MQ=255)
                                                                                 gacgtctgagaTAAGGCATTTTACTTTGTTGCGTTCAGTGCTTTCAGTGAAAAGAAAAAACTCCGGTTGCATGTCTCTAGCTCTTATCGAATTTCTGAAAATGAGATCGGTACATCAGTGAAATTTTTCACCATTGCCTACAACTTAtt                                                                   <  5:289726/142‑1 (MQ=255)
                                                                                 gACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGa                                                                                                              >  7:137067/1‑106 (MQ=255)
                                                                                 gACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGa                                                                                                              <  8:137067/106‑1 (MQ=255)
                                                                                  aCGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTAtt                                                                   >  1:289666/1‑148 (MQ=255)
                                                                                  aCGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTg                                                                  <  4:145498/149‑1 (MQ=255)
                                                                                   cGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTga                                                                 <  7:379067/149‑1 (MQ=255)
                                                                                    ggaggaGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTAtt                                                                   >  7:171477/1‑146 (MQ=255)
                                                                                    ggaggaGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTgag                                                                <  8:171477/149‑1 (MQ=255)
                                                                                     gaggagATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTgaga                                                               <  6:113117/149‑1 (MQ=255)
                                                                                      aggagATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGaa                                                              <  2:289666/149‑1 (MQ=255)
                                                                                        gagaTAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGaaa                                                             >  5:150262/1‑148 (MQ=255)
                                                                                        gagaTAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGATTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGaaa                                                             >  4:127657/1‑148 (MQ=255)
                                                                                        gagaTAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGATTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGaaa                                                             >  4:122275/1‑148 (MQ=255)
                                                                                            tAAGGCATTTTTCTTTGTTGCGTTCAGTGCTTTCAGGGAAAATAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCACATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGATATAag                                                         <  1:366812/148‑1 (MQ=255)
                                                                                              aGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCt                                                                                                                                    <  3:170706/71‑1 (MQ=255)
                                                                                              aGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCt                                                                                                                                    >  4:170706/1‑71 (MQ=255)
                                                                                              aGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAagcag                                                      >  8:124006/1‑149 (MQ=255)
                                                                                               ggCATTTTACTTTGTTGCGGTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAagcagc                                                     <  8:156971/149‑1 (MQ=255)
                                                                                                  aTTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTc                                                  >  4:48325/1‑149 (MQ=255)
                                                                                                   ttttACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTcc                                                 >  4:120476/1‑149 (MQ=255)
                                                                                                   ttttACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTcc                                                 >  2:142911/1‑149 (MQ=255)
                                                                                                         tttGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGcc                                           >  8:360195/1‑149 (MQ=255)
                                                                                                         tttGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGcc                                           >  3:48627/1‑149 (MQ=255)
                                                                                                         tttGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGcc                                           >  1:320190/1‑149 (MQ=255)
                                                                                                             ttgCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTc                                        >  3:151936/1‑148 (MQ=255)
                                                                                                             ttgCGGTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCATCAGCCCTc                                        >  6:226950/1‑148 (MQ=255)
                                                                                                                cGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCaaaa                                    >  5:126424/1‑149 (MQ=255)
                                                                                                                cGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCaaaa                                    >  7:264961/1‑149 (MQ=255)
                                                                                                                 gTTCAGGGCTTTCAGGGAAAAGAAAAACCTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATGTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCaaaa                                    >  4:241159/1‑148 (MQ=255)
                                                                                                                  ttCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGATTCCTCAGCCCTCAAAAt                                   >  7:200911/1‑148 (MQ=255)
                                                                                                                  ttCAGGGCCTTCCGCGAAAAGAAAAAACTCCGTATGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCCGCCCTCAAAATa                                  <  6:51062/149‑1 (MQ=255)
                                                                                                                     aGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAAc                                <  7:76682/148‑1 (MQ=255)
                                                                                                                      gggCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACa                               >  6:383233/1‑148 (MQ=255)
                                                                                                                        gCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCGATTTCTGAATAACAGAAAGGTATATCTGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACaaaa                            <  8:46051/149‑1 (MQ=255)
                                                                                                                        gCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACaaaa                            <  1:214136/149‑1 (MQ=255)
                                                                                                                        gCTTTCAGGGAAAAGAAAAAACTCCGATTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACaaaa                            <  3:127657/149‑1 (MQ=255)
                                                                                                                        gCTTTCAGGGAAAAGAAAAAACTCCGATTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACaaaa                            <  3:122275/149‑1 (MQ=255)
                                                                                                                         cTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGAAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAAc                           >  5:76429/1‑149 (MQ=255)
                                                                                                                          tttCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAAcc                          >  1:255242/1‑149 (MQ=255)
                                                                                                                          tttCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATATATCACCATTGCCTACAACTTTTTGAGAAATTAGCAGCTTCCTCAGCCCTCAAAATAACAAAAcc                          >  2:267029/1‑149 (MQ=255)
                                                                                                                            tCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAAcc                          <  6:150262/147‑1 (MQ=255)
                                                                                                                              aGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAACCCAcc                      >  6:240490/1‑149 (MQ=255)
                                                                                                                              aGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAACCCAcc                      <  7:124006/149‑1 (MQ=255)
                                                                                                                                ggAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAACCCACCt                     >  7:272578/1‑148 (MQ=255)
                                                                                                                                  aaaagaaaaAACTCCTGTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAACCCACCTTaa                  <  5:144048/149‑1 (MQ=255)
                                                                                                                                   aaagaaaaAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAACCCACCTTAAg                 <  4:85395/149‑1 (MQ=255)
                                                                                                                                    aagaaaaAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAAc                           <  8:145372/138‑1 (MQ=255)
                                                                                                                                    aagaaaaAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAAc                           >  7:145372/1‑138 (MQ=255)
                                                                                                                                      gaaaaAACTCCGATTGCATGTAACTAGCGCTTATCCCAATTCTGAAACACAGAAATGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAACCCACCTTAAGGTg              <  2:207676/149‑1 (MQ=255)
                                                                                                                                       aaaaaaCTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAACCCACCTTAAGGTgg             >  5:106384/1‑149 (MQ=255)
                                                                                                                                       aaaaaaCTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAACCCACCTTAAGGTgg             >  6:183990/1‑149 (MQ=255)
                                                                                                                                           aaCTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAACCCACCTTAAGGTGGGttt         <  8:367658/149‑1 (MQ=255)
                                                                                                                                             cTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAACCCACCTTAAGGTGGGttttg       <  7:84959/149‑3 (MQ=255)
                                                                                                                                                cGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAACCCACCTTAAGGTGGGttttgtt     <  8:272578/148‑5 (MQ=255)
                                                                                                                                                  tttGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAGAATAACAAAACCCACCTTAAGGTGGGTTTCGCCaga  <  4:48627/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCATGTAACTAGCGCTTATCCCATTTCTGAAAAACAGAAAGGTAAATCAGTGAAATTTTTCACCATTGCCTACAACTTATTGAGAAATAAGCAGCTTCCTCAGCCCTCAAAATAACAAAACCCACCTTAAGGTGGGTTTCGCCAGA  >  NC_000913/4190062‑4190356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: