Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 566,756 G→C 100% pseudogene (73/93 nt) renD → pseudogene, DLP12 prophage;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913566,7560GC97.7% 5302.3 / 66.5 1848pseudogene (73/93 nt)renDpseudogene, DLP12 prophage;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base G (21/22);  new base C (970/830);  total (995/853)
Fisher's exact test for biased strand distribution p-value = 5.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 1849 total aligned reads displayed.

ACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGCGCAACCGTAATCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCT  >  NC_000913/566609‑566892
                                                                                                                                                   |                                                                                                                                        
  ctctAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTaa                                                                                                                                       <  4:244511/149‑1 (MQ=255)
  ctctAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTaa                                                                                                                                       >  1:293478/1‑149 (MQ=255)
  ctctAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATAAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTaa                                                                                                                                       >  1:77215/1‑149 (MQ=255)
   tctAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTaa                                                                                                                                       >  2:370498/1‑148 (MQ=255)
   tctAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTaa                                                                                                                                       >  3:149183/1‑148 (MQ=255)
   tctAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGAATGAAAGGATCAAGTGTATGAAGGGCAAAGAGGCAATTATTCATTACATGGGGAAGCATAATAGCTTCAGTGCGCCGGACGTTGCCGCGCTaa                                                                                                                                       >  6:279470/1‑148 (MQ=255)
     tAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAAc                                                                                                                                      >  8:239958/1‑147 (MQ=255)
     tAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAg                                                                                                                                    >  1:242482/1‑149 (MQ=255)
     tAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAg                                                                                                                                    >  3:176830/1‑149 (MQ=255)
     tAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAg                                                                                                                                    >  2:249716/1‑149 (MQ=255)
       aaTCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACa                                                                                                                                     <  7:363282/146‑1 (MQ=255)
       aaTCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAg                                                                                                                                    <  3:99309/147‑1 (MQ=255)
       aaTCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGgc                                                                                                                                  <  7:208459/149‑1 (MQ=255)
       aaTCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTAGGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACa                                                                                                                                     <  3:112089/146‑1 (MQ=255)
       aaTCGTGCACAGGCTCTGGCGAAGAGCGCAGAAATCAAAGCTAAGTTCCGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGCGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGgc                                                                                                                                  <  5:67983/149‑1 (MQ=255)
       aaTCGAGCACAGGCTCTGGCGAAGCCCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAGGAGGCAATTATTCATTACCTGGGGACGCATAATCGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAg                                                                                                                                    <  2:273941/147‑1 (MQ=255)
        aTCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGgcg                                                                                                                                 >  2:348827/1‑149 (MQ=255)
         tCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGgcgc                                                                                                                                <  6:307659/149‑1 (MQ=255)
         tCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGgcg                                                                                                                                 >  5:138407/1‑148 (MQ=255)
         tCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCAAACAGgcg                                                                                                                                 >  8:110755/1‑148 (MQ=255)
           gTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCaa                                                                                                                              <  8:18983/149‑1 (MQ=255)
            tGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCaa                                                                                                                              >  4:183481/1‑148 (MQ=255)
            tGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCaa                                                                                                                              >  1:102130/1‑148 (MQ=255)
              cacaGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAAca                                                                                                                            <  1:351892/148‑2 (MQ=255)
              cacaGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACAGAAAGGAGAAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAAcag                                                                                                                           >  3:227332/1‑147 (MQ=255)
               acaGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAAcag                                                                                                                           >  7:149131/1‑146 (MQ=255)
               acaGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGt                                                                                                                          <  6:332742/149‑1 (MQ=255)
                 aGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAA                                                                                                                        <  3:198663‑M1/149‑1 (MQ=255)
                  ggCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAc                                                                                                                       <  1:387215‑M1/149‑2 (MQ=255)
                    ctctGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAc                                                                                                                       >  8:43041‑M1/1‑146 (MQ=255)
                     tctGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccag                                                                                                                    >  7:102709‑M1/1‑145 (MQ=255)
                       tGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGCCTGAAAGGAGCAAGTGTATGCCGGGCAAAGAGGCAATTTTTCATTACATGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTacccagca                                                                                                                  >  1:132282/1‑142 (MQ=255)
                       tGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagca                                                                                                                  >  5:315976‑M1/1‑143 (MQ=255)
                       tGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagca                                                                                                                  >  4:129163‑M1/1‑143 (MQ=255)
                         gCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagca                                                                                                                  <  5:228292‑M1/147‑7 (MQ=255)
                          cGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataa                                                                                                               <  6:55758‑M1/149‑10 (MQ=255)
                           gAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaa                                                                                                              >  1:50922‑M1/1‑139 (MQ=255)
                           gAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaa                                                                                                              >  2:39238‑M1/1‑139 (MQ=255)
                           gAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaa                                                                                                              >  3:331557‑M1/1‑139 (MQ=255)
                            aaGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaat                                                                                                             <  8:358976‑M1/149‑12 (MQ=255)
                              gATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatca                                                                                                           <  5:51288‑M1/149‑14 (MQ=255)
                               aTCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcag                                                                                                          <  8:169142‑M1/149‑15 (MQ=255)
                                tCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcagg                                                                                                         >  2:90757‑M1/1‑134 (MQ=255)
                                  gCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggcc                                                                                                       >  1:31732‑M1/1‑132 (MQ=255)
                                  gCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggcc                                                                                                       >  2:143401‑M1/1‑132 (MQ=255)
                                  gCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggc                                                                                                        <  1:209196‑M1/148‑17 (MQ=255)
                                  gCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGACAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggcc                                                                                                       >  6:115343‑M1/1‑132 (MQ=255)
                                   cAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccg                                                                                                      >  1:176302‑M1/1‑131 (MQ=255)
                                    aGAAATCAAAGCTATGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCTATTATTCATTTCCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgc                                                                                                     >  5:259840‑M1/1‑130 (MQ=255)
                                    aGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgc                                                                                                     >  4:217535‑M1/1‑130 (MQ=255)
                                    aGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgc                                                                                                     >  2:305945‑M1/1‑130 (MQ=255)
                                        atcaaagctAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggct                                                                                                 <  8:379936‑M1/146‑24 (MQ=255)
                                        atcaaagctAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggcc                                                                                                       <  6:176198‑M1/140‑18 (MQ=255)
                                         tcaaagcTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggcta                                                                                                <  6:205745‑M1/147‑25 (MQ=255)
                                           aaaGCTAAGTTCGGACTGAAAGTAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaa                                                                                               >  7:237111‑M1/1‑123 (MQ=255)
                                           aaaGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatca                                                                                                           >  8:6759‑M1/1‑123 (MQ=255)
                                            aaGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaa                                                                                             <  4:14834‑M1/149‑28 (MQ=255)
                                             aGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaat                                                                                            >  5:98198‑M1/1‑121 (MQ=255)
                                              gCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatg                                                                                           <  2:218421‑M1/149‑30 (MQ=255)
                                               cTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatgg                                                                                          >  4:315408‑M1/1‑119 (MQ=255)
                                                tAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatgg                                                                                          >  7:123620‑M1/1‑118 (MQ=255)
                                                tAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatgg                                                                                          >  4:94511‑M1/1‑118 (MQ=255)
                                                 aaGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggca                                                                                        >  4:284735‑M1/1‑117 (MQ=255)
                                                  aGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcac                                                                                       <  5:282590‑M1/149‑34 (MQ=255)
                                                      cGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataatcaggccgcggctaaaatggcacgggc                                                                                    <  2:19644‑M1/148‑37 (MQ=255)
                                                        gACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcatacatcaggccgcggctaaaatggcccgggcag                                                                                 <  3:295212‑M1/149‑40 (MQ=255)
                                                          cTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggt                                                                               <  4:370240‑M1/149‑42 (MQ=255)
                                                          cTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggt                                                                               <  8:300509‑M1/149‑42 (MQ=255)
                                                           tGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGAATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggcagcggctaaaatggcacgggcaggtc                                                                              >  5:345343‑M1/1‑107 (MQ=255)
                                                             aaaGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtctt                                                                            >  7:282710‑M1/1‑105 (MQ=255)
                                                             aaaGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtctg                                                                            >  7:83950‑M1/1‑105 (MQ=255)
                                                             aaaGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCAGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgtgcaggtctt                                                                            >  3:51536‑M1/1‑105 (MQ=255)
                                                              aaGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttc                                                                           >  6:364926‑M1/1‑104 (MQ=255)
                                                               aGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAc                                                                                                                       <  7:328366‑M1/104‑2 (MQ=255)
                                                                ggAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctg                                                                         <  5:166099‑M1/149‑48 (MQ=255)
                                                                 gAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttct                                                                          >  8:142306‑M1/1‑101 (MQ=255)
                                                                 gAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggct                                                                                                 >  6:7882‑M1/1‑101 (MQ=255)
                                                                  aGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggt                                                                       <  6:143870‑M1/149‑50 (MQ=255)
                                                                    cAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggtta                                                                     <  7:383461‑M1/149‑52 (MQ=255)
                                                                     aaGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttat                                                                    >  8:272574‑M1/1‑97 (MQ=255)
                                                                     aaGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatg                                                                                           >  4:75706‑M1/1‑97 (MQ=255)
                                                                     aaGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatg                                                                                           <  3:75706‑M1/126‑30 (MQ=255)
                                                                          tATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaa                                                                <  2:163871‑M1/148‑57 (MQ=255)
                                                                          tATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgaggctaaaatggcacgggcaggtcttctggttatcgaa                                                                <  5:203257‑M1/148‑57 (MQ=255)
                                                                            tGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggcc                                                                                                       >  4:340488‑M1/1‑90 (MQ=255)
                                                                            tGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGCGCAACCGTAATCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCagag                                                              <  6:237980/148‑1 (MQ=255)
                                                                              aCGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAgg                                                                                                                                   >  5:11148/1‑77 (MQ=255)
                                                                              aCGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaa                                                           <  7:185138‑M1/149‑62 (MQ=255)
                                                                               cGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaag                                                          >  8:215291‑M1/1‑87 (MQ=255)
                                                                               cGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaag                                                          <  5:37174‑M1/149‑63 (MQ=255)
                                                                               cGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggct                                                                                                 <  8:325228‑M1/110‑24 (MQ=255)
                                                                                  gCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtc                                                       >  2:63003‑M1/1‑84 (MQ=255)
                                                                                   cAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtct                                                      <  7:303024‑M1/149‑67 (MQ=255)
                                                                                    aaaGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctg                                                     <  2:112002‑M1/149‑68 (MQ=255)
                                                                                      agagGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggc                                                   >  3:356514‑M1/1‑80 (MQ=255)
                                                                                        agGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcg                                                  >  7:55205‑M1/1‑78 (MQ=255)
                                                                                           cAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGCGCGACCGTAATCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACaa                                              <  3:266373/149‑1 (MQ=255)
                                                                                                                                    ccggacGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaacggtgtattaccggtttgctaccagggaagaacgggaaggaaa     <  7:253660‑M1/147‑116 (MQ=255)
                                                                                                                                    ccggacGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaacggtgtattaccggtttgctaccagggaagaacgggaaggaaa     <  1:150419‑M1/147‑116 (MQ=255)
                                                                                                                                     cggaCGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaacggtgtattaccggtttgctaccagggaagaacgggaaggaaag    <  1:270769‑M1/148‑117 (MQ=255)
                                                                                                                                                   |                                                                                                                                        
ACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGCGCAACCGTAATCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCT  >  NC_000913/566609‑566892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: