Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 579,134 T→C 100% intergenic (‑241/+50) borD ← / ← ybcV DLP12 prophage; putative lipoprotein/DLP12 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913579,1340TC98.8% 10093.2 / 24.9 3537intergenic (‑241/+50)borD/ybcVDLP12 prophage; putative lipoprotein/DLP12 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (22/23);  new base C (1727/1742);  total (1759/1778)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.96e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 3917 total aligned reads displayed.

ATATTTATTAATGTATGCCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTT  >  NC_000913/578987‑579280
                                                                                                                                                   |                                                                                                                                                  
  atttattAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGcatta                                                                                                                                                >  6:193030/1‑149 (MQ=255)
   tttattAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGcattat                                                                                                                                               >  3:252941/1‑149 (MQ=255)
   tttattAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGcattat                                                                                                                                               <  1:352878/149‑1 (MQ=255)
    ttattaATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCAATATg                                                                                                                                              >  1:100015/1‑149 (MQ=255)
               tgtcAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCg                                                                                                                                   >  3:107425/4‑149 (MQ=255)
               tgtcAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCg                                                                                                                                   >  5:277086/4‑149 (MQ=255)
                   aGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGCCGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGc                                                                                                                               >  5:7963/1‑149 (MQ=255)
                   aGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGcc                                                                                                                                            >  4:98894/1‑136 (MQ=255)
                    ggTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCt                                                                                                                              <  6:252209/149‑1 (MQ=255)
                     gTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCt                                                                                                                              >  5:224781/1‑148 (MQ=255)
                        cGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGc                                                                                                                               <  8:75487/144‑1 (MQ=255)
                        cGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGac                                                                                                                           <  2:853/148‑1 (MQ=255)
                         gATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGacac                                                                                                                         <  2:248623/149‑1 (MQ=255)
                         gATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGacac                                                                                                                         >  4:68422/1‑149 (MQ=255)
                         gATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGacac                                                                                                                         <  7:288782/149‑1 (MQ=255)
                           tGAATCGTCATTGTATTCCCGTATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACgg                                                                                                                       <  5:176478/149‑1 (MQ=255)
                               tCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGgaag                                                                                                                    >  2:56613/1‑148 (MQ=255)
                                 gTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGaaa                                                                                                                 >  2:331043/1‑149 (MQ=255)
                                 gTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGaaa                                                                                                                 >  7:81219/1‑149 (MQ=255)
                                  tCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAAc                                                                                                                >  1:160414/1‑149 (MQ=255)
                                    aTTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCg                                                                                                              >  3:63358/1‑149 (MQ=255)
                                    aTTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCg                                                                                                              >  4:11629/1‑149 (MQ=255)
                                     ttGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCgg                                                                                                             >  4:347181/1‑149 (MQ=255)
                                       gtcttCCCTGATTAACTATGTCCTCTGCCCTGAGGGGGCACGTCTCTGCGGGAGGGTCAGGGAATAATTAAACCGTTGCACTCAGGGTTTAGCGCGTTCACGTATTGCATTATGCCAACGACCCGGGGCTGACACGGAAGAAACCGGa                                                                                                            <  8:276423/145‑1 (MQ=255)
                                        tATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTGCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGAc                                                                                                           <  3:130750/148‑1 (MQ=255)
                                          ttCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGtt                                                                                                        <  4:178717/149‑1 (MQ=255)
                                             ccGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTAt                                                                                                      <  7:184509/148‑1 (MQ=255)
                                             ccGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTAt                                                                                                      >  1:21689/1‑148 (MQ=255)
                                             ccGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGCTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTAt                                                                                                      >  2:307634/1‑148 (MQ=255)
                                                    aaCTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAg                                                                                               <  1:326461/148‑1 (MQ=255)
                                                      cTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGt                                                                                            <  3:308473/149‑1 (MQ=255)
                                                      cTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGt                                                                                            >  7:124845/1‑149 (MQ=255)
                                                      cTATGTCCACAGCCCTGACGGGGAAATTATCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGCGTTTAGCGCGTACACGTATTGCTTTATGCCCAAGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATAATTTAGc                                                                                              >  7:336866/1‑147 (MQ=255)
                                                         tGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACCGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTg                                                                                           <  3:37440/147‑1 (MQ=255)
                                                         tGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGa                                                                                         <  2:380827/149‑1 (MQ=255)
                                                           tCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTCCCCGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGa                                                                                         >  1:132041/1‑147 (MQ=255)
                                                           tCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGaaa                                                                                       <  3:280971/149‑1 (MQ=255)
                                                             cacaGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGaaa                                                                                       <  5:363126/148‑1 (MQ=255)
                                                                aGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGAttt                                                                                  <  8:360651/149‑1 (MQ=255)
                                                                aGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGAttt                                                                                  <  8:335063/149‑1 (MQ=255)
                                                                aGCCCTGACCGGGAACTTCTCTGCGGGCGTGTCCTGGAATAATTAACACCATGCACACAGGGTTAAGCGCGTACACGCATAGCAATATGCCAACGCCCCGGCGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGAttt                                                                                  <  6:105371/149‑1 (MQ=255)
                                                                 gCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTa                                                                                                >  2:110071/1‑134 (MQ=255)
                                                                   ccTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGCAACCGGACGTTATGATTTAGCGTGGAAAGATTtgtg                                                                               <  1:280830/149‑1 (MQ=255)
                                                                     tGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTtgtg                                                                               >  7:154974/1‑147 (MQ=255)
                                                                     tGACGGGGAACTTCTCTGCGGGAGTGTCAGGGAATAATTAAAACGATGCACACATGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTAAGATTTAGCGTGGAAAGATTagtg                                                                               >  6:2777/1‑147 (MQ=255)
                                                                         ggggAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAgtgt                                                                         >  5:146537/1‑149 (MQ=255)
                                                                          gggAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTtgt                                                                                >  2:280984/1‑141 (MQ=255)
                                                                           gggaCTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGGTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGGTc                                                                       >  6:96827/4‑149 (MQ=255)
                                                                            gAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCt                                                                      <  3:168233/149‑1 (MQ=255)
                                                                               cTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGaa                                                                   <  8:163243/149‑1 (MQ=255)
                                                                                  ctctGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTTAATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATg                                                                 <  7:243406/148‑1 (MQ=255)
                                                                                   tctGCGGAAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTTGAAAGATTTGTGTAGTGTTCTGAATGct                                                               >  4:315770/1‑149 (MQ=255)
                                                                                       cgtgAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCATCGCCCCGGTGCTGACACGGTAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTTTGTAGTGTTCTGAATGCTCTCa                                                           <  8:110672/146‑1 (MQ=255)
                                                                                       cGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGctct                                                             <  2:354539/147‑1 (MQ=255)
                                                                                       cGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGctct                                                             <  1:85176/147‑1 (MQ=255)
                                                                                       cGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCa                                                           <  5:199686/149‑1 (MQ=255)
                                                                                       cGGGAGTGTCCGGAAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCa                                                           <  5:251124/149‑1 (MQ=255)
                                                                                              gtCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGACGAAACCGGCCGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATa                                                    <  4:150216/149‑1 (MQ=255)
                                                                                               tCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCa                                                           <  1:187623/141‑1 (MQ=255)
                                                                                               tCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATa                                                    <  8:30369/148‑1 (MQ=255)
                                                                                                  gggAATAATTAAAACGATGCACACAGGGTTTAGCGCCAACACGTATAGCATTATGCCAACGCCCCGGTGCTGCCACGGAAGAAACCGGACGAAATGAGTTAGCGTGGAAAGATTAGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTaa                                                >  6:309714/1‑149 (MQ=255)
                                                                                                     aataatTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATg                                              >  6:349532/1‑148 (MQ=255)
                                                                                                      ataatTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGGTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGaa                                            <  6:4137/149‑1 (MQ=255)
                                                                                                         aattaaaaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAAt                                           >  7:214758/5‑148 (MQ=255)
                                                                                                          attaaaaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTa                                         >  8:257004/4‑149 (MQ=255)
                                                                                                             aaaaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCTGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATc                                       <  5:336348/148‑1 (MQ=255)
                                                                                                              aaaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGGTCTGAATGCTCTCAGTAAATAGTAATGAATTATCaa                                     <  6:223059/149‑1 (MQ=255)
                                                                                                               aaCGCTGCCCACAGGGTTTCGCGCGTACACGTATTGCATTCTGCCCAAGCCCCGTTGCTGACACGGAAGAAACCGGACGGTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGAAAATAGTAATGAATTATCaaa                                    <  6:73145/149‑1 (MQ=25)
                                                                                                               aaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCaaa                                    <  6:135206/149‑1 (MQ=255)
                                                                                                               aaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCaaa                                    <  5:119508/149‑1 (MQ=255)
                                                                                                                aCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCTGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAg                                   <  1:244472/149‑1 (MQ=255)
                                                                                                                 cGATGCACACATCGGTTAGCTCGTACACGAATTGCATTATGCCAACTCCCCGGTGCTGACACGGAAGAAAACTGAAGTTATTATTTAGATTGGAAAGAGTTGTTTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATAAAAgg                                  <  4:289110/149‑1 (MQ=25)
                                                                                                                 cGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAgg                                  >  1:300375/1‑149 (MQ=255)
                                                                                                                  gATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCt                                                                      >  7:309400/1‑112 (MQ=255)
                                                                                                                   aTGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGta                                <  4:229072/149‑1 (MQ=255)
                                                                                                                       acacaGGGTTTAGCGCGTACACGTATTTCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCa                                                           <  7:55134/118‑1 (MQ=255)
                                                                                                                       acacaGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAg                             >  3:222742/1‑148 (MQ=255)
                                                                                                                       acacaGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAg                             >  7:268296/1‑148 (MQ=255)
                                                                                                                       acacaGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAg                             >  8:50055/1‑148 (MQ=255)
                                                                                                                         acaGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGCCCCCGGACGTGATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTaa                          >  1:380253/1‑149 (MQ=25)
                                                                                                                             ggTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCt                                                                      <  3:95812/101‑1 (MQ=255)
                                                                                                                             ggTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATc                      >  5:296522/1‑149 (MQ=255)
                                                                                                                             ggTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATc                      <  7:359431/149‑1 (MQ=255)
                                                                                                                              gTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCt                     >  4:40904/1‑149 (MQ=255)
                                                                                                                                  aGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACTGAAGAAACCGGACGTTATGATTTAGCGTGTAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCtttt                  <  4:201642/148‑1 (MQ=25)
                                                                                                                                  aGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCtttt                  <  2:192665/148‑1 (MQ=255)
                                                                                                                                  aGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCtttt                  <  4:258328/148‑1 (MQ=255)
                                                                                                                                   gcgcGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTAt                <  7:387023/149‑1 (MQ=255)
                                                                                                                                   gcgcGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTAt                >  2:220096/1‑149 (MQ=255)
                                                                                                                                    cgcgTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATg               <  2:140884/149‑1 (MQ=255)
                                                                                                                                     gcgTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGt              >  8:385124/1‑149 (MQ=255)
                                                                                                                                         acacGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATATTTTATGTTcat          >  8:195955/1‑147 (MQ=255)
                                                                                                                                         acacGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGCTATAGTAATATCTTTTATGTTcat          >  3:343460/1‑147 (MQ=255)
                                                                                                                                         acacGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTTCAAAGGTATAGTAATATCTTTTATGTTCATg         >  8:132354/1‑149 (MQ=21)
                                                                                                                                          cacgtattgcattATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTcat          <  8:226698/145‑3 (MQ=255)
                                                                                                                                          cacgtattgcattATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTcat          <  6:165277/145‑3 (MQ=255)
                                                                                                                                           acgtattgcattATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATg         >  3:196658/3‑148 (MQ=255)
                                                                                                                                           acgtattgcattATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAATAATATCTTTTATGTTCATg         >  2:167755/3‑148 (MQ=255)
                                                                                                                                             gtattgcattATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTca           <  1:55047/144‑2 (MQ=255)
                                                                                                                                             gtattgcattATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGat      <  5:51783/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATATTTATTAATGTATGCCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTT  >  NC_000913/578987‑579280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: