Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 579,146 A→G 100% intergenic (‑253/+38) borD ← / ← ybcV DLP12 prophage; putative lipoprotein/DLP12 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913579,1460AG98.9% 11594.7 / 25.6 4064intergenic (‑253/+38)borD/ybcVDLP12 prophage; putative lipoprotein/DLP12 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (26/21);  new base G (2043/1947);  total (2083/1981)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 4065 total aligned reads displayed.

GTATGCCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCG  >  NC_000913/578999‑579291
                                                                                                                                                   |                                                                                                                                                 
gTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTTTTCCAACGcc                                                                                                                                                 >  7:370820/1‑149 (MQ=255)
gTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGcc                                                                                                                                                 >  7:198186/1‑149 (MQ=255)
     tcAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCgg                                                                                                                                             <  3:75010/147‑1 (MQ=255)
     tcAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGt                                                                                                                                            >  5:52364/2‑149 (MQ=255)
             gATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGacac                                                                                                                                    >  4:208572/1‑149 (MQ=255)
                 aaTCGTCATTGTATTCCCGGATTAACTATGTCCACAGGCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGTAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGgaa                                                                                                                                <  3:356695/149‑1 (MQ=255)
                 aaTCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGATCTTCTCTGCGGGAGTGTCGGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGGATTGCATTATGCCAACGCCCCGGTGCTGACACGgaa                                                                                                                                <  6:135765/149‑1 (MQ=255)
                 aaTCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGATTAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTTCATTATGCCAACGCCCCGGTGCTGACACGgaa                                                                                                                                <  2:121941/149‑1 (MQ=255)
                 aaTCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGGTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGgaa                                                                                                                                <  2:364457/149‑1 (MQ=255)
                      tCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAAc                                                                                                                           >  4:50158/1‑149 (MQ=255)
                      tCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAAc                                                                                                                           <  2:256094/149‑1 (MQ=255)
                         ttGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCg                                                                                                                         >  1:182349/1‑148 (MQ=255)
                          tGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGgaagaa                                                                                                                             <  3:204736/143‑1 (MQ=255)
                           gTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGAc                                                                                                                      >  4:32965/1‑149 (MQ=255)
                              ttCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGgaa                                                                                                                                <  5:299015/136‑1 (MQ=255)
                                 ccGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATg                                                                                                                <  1:61303/149‑1 (MQ=255)
                                 ccGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGGCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATg                                                                                                                <  2:176557/149‑1 (MQ=255)
                                  cGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGa                                                                                                               >  6:37139/1‑149 (MQ=255)
                                  cGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGa                                                                                                               >  8:383998/1‑149 (MQ=255)
                                      tttaCTATGTCCACAGCCCTGACGGGGAACTTCTCTTCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTa                                                                                                           <  7:168726/146‑1 (MQ=255)
                                      ttAACTATGTCCACAGCCCTGACGGGTAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTa                                                                                                           <  4:27299/149‑1 (MQ=255)
                                         aCTATGTCCACGGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGc                                                                                                         <  8:322090/148‑1 (MQ=255)
                                          cTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGt                                                                                                       <  4:7705/149‑1 (MQ=255)
                                          cTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGt                                                                                                       <  6:56823/149‑1 (MQ=255)
                                           tATGTCCACAGCCCTGACGCGGAACTTCTCTGCGGGAGTGTCCGTGAATAATTAAAAAGATGCACACAGGGTTTAGCGCGTACACTAATTGCATTATGCCAACGCCCCGCTGCTCACACGGAAGAAACCCGACAATATCATTAAGCGTg                                                                                                      >  8:124923/1‑149 (MQ=255)
                                               tCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGACAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGa                                                                                                    >  5:104101/1‑147 (MQ=255)
                                                ccACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGa                                                                                                    >  6:272080/1‑146 (MQ=255)
                                                 caccGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAACCGATGCACACAGGGTTTAGCGCGTAAACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGa                                                                                                    >  7:275794/1‑145 (MQ=255)
                                                 cacaGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGCAACCGGACGTTATGATTTAGCGTGGa                                                                                                    >  1:306678/1‑145 (MQ=255)
                                                  acaGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACAAGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGAt                                                                                               <  8:353692/149‑1 (MQ=255)
                                                    aGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGAttt                                                                                             <  5:279334/149‑1 (MQ=255)
                                                      cccTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTtgt                                                                                           >  7:255462/1‑149 (MQ=255)
                                                       ccTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGCAACCGGACGTTATGATTTAGCGTGGAAAGATTtgtg                                                                                          <  1:280830/149‑1 (MQ=255)
                                                       ccTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGAGGAAAGATTtgtg                                                                                          <  8:104275/149‑1 (MQ=255)
                                                           aCGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTa                                                                                        <  1:331043/147‑1 (MQ=255)
                                                           aCGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAgt                                                                                      <  1:360402/149‑1 (MQ=255)
                                                             ggggAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAgtgt                                                                                    >  2:202621/1‑149 (MQ=255)
                                                              gggAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGtt                                                                                   <  8:182297/149‑1 (MQ=255)
                                                                 aaCTTCTCTGCGGGAGTGTCCGGTAATAATTAAAACTCTGCACACAGGGATTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATCATTTAGCGCGGAAAGATTTGTGTAGTGTTCTg                                                                                <  6:220221/149‑1 (MQ=255)
                                                                  aCTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACAAGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGtt                                                                                   >  6:19148/1‑145 (MQ=255)
                                                                   cTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGaa                                                                              >  1:157773/1‑149 (MQ=255)
                                                                    ttCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAAt                                                                             >  7:137665/1‑149 (MQ=255)
                                                                      ctctGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACCCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTAGAAAGATTTGTGTAGTGTTCTGAATGc                                                                           >  4:301910/1‑149 (MQ=255)
                                                                       tctGCGGGAGTGTGCGGGAATTATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCATACGCCCAGGTGATGAACCGGAAGAAACCGGACGGTATGATTTAGCGTGGACAGATTTGGGTACTGTTCTGAATGct                                                                          >  2:93274/1‑149 (MQ=255)
                                                                           cGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCa                                                                      <  3:320823/149‑1 (MQ=255)
                                                                            gggTGTGTCCGGTAATAATAAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGTAAAGATTTGTGTAGTGTTCTGAATGCTCTCAg                                                                     <  6:338867/149‑1 (MQ=255)
                                                                            gggAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACCCGTATTGCATTATGCCAACGCCCCGGTGCTGCCACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAg                                                                     >  3:291920/1‑149 (MQ=255)
                                                                              gAGTGTCCGGTAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTa                                                                   <  8:295617/149‑1 (MQ=255)
                                                                                  gtCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGACGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATa                                                               <  6:294344/149‑1 (MQ=255)
                                                                                  gtCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATa                                                               <  2:228511/149‑1 (MQ=255)
                                                                                   tCCTGGAATTATTAAAACGATGCACACAGGGTTTAGCTCGTACACGTATTGCTTTATGCCAACGCCCCGGTGCTGACACGGAAGAAACAGGACGTTATGATTTAGCGTGGAAAGATATGTGTAGTGTTCTGAATGCTCTCAGTAAATa                                                               <  5:182043/148‑1 (MQ=255)
                                                                                   tCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCagtaagta                                                               <  3:177595/148‑1 (MQ=255)
                                                                                   tCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAg                                                              >  4:109078/1‑149 (MQ=255)
                                                                                    ccGGGAATAATTAAAACGATGCACACAGGGTTTAGCCCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGGTATGATTTAGCGTGGAAAGAGTTGTGTAGTGTTCTGAATGCTCGCAGTAAATAg                                                              >  2:64621/1‑148 (MQ=255)
                                                                                     cGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGt                                                             <  4:357924/148‑1 (MQ=255)
                                                                                      gggAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTaa                                                           <  7:325236/149‑1 (MQ=255)
                                                                                      gggAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTaa                                                           <  7:69276/149‑1 (MQ=255)
                                                                                       ggAATAATTAAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTCTGCCAACGCCCCTGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAAt                                                          <  2:311949/149‑1 (MQ=255)
                                                                                             aattaaaaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCg                                                                                                        >  2:149418/5‑98 (MQ=255)
                                                                                              attaaaaCGATGCACACAGGGGTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGATATGTTTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCGCTCAGTAAATAGTAATGAATTa                                                    <  2:340262/146‑1 (MQ=37)
                                                                                                 aaaaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATc                                                  >  7:296611/1‑148 (MQ=255)
                                                                                                 aaaaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATc                                                  >  3:261590/1‑148 (MQ=255)
                                                                                                 aaaaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATc                                                  >  5:79544/1‑148 (MQ=255)
                                                                                                 aaaaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATc                                                  <  4:242811/148‑1 (MQ=255)
                                                                                                  aaaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTa                                                            <  3:14593/137‑1 (MQ=255)
                                                                                                   aaCGATGCCCACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCaaa                                               <  6:108925/149‑1 (MQ=255)
                                                                                                   aaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCa                                                 >  5:154428/1‑147 (MQ=255)
                                                                                                    aCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAATAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAg                                              <  5:366301/149‑1 (MQ=255)
                                                                                                     cGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAgg                                             >  5:338202/1‑149 (MQ=255)
                                                                                                       aTGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAg                                                                     >  8:7435/1‑123 (MQ=255)
                                                                                                       aTGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGta                                           <  1:101764/149‑1 (MQ=255)
                                                                                                       aTGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGta                                           >  8:48182/1‑149 (MQ=255)
                                                                                                          cacacaGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAACCCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAg                                        <  6:78953/149‑1 (MQ=255)
                                                                                                          cacacaGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAg                                        <  8:240462/149‑1 (MQ=255)
                                                                                                           acacaGGTTTTAGCGCGTACACGTAATGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCt                                                                                 <  7:10947/107‑1 (MQ=255)
                                                                                                           acacaGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAg                                        >  7:223411/1‑148 (MQ=255)
                                                                                                           acacaGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAg                                        >  5:252168/1‑148 (MQ=255)
                                                                                                             acaGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATa                                                               <  3:49379/123‑1 (MQ=255)
                                                                                                             acaGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTaa                                     >  8:155087/1‑149 (MQ=255)
                                                                                                                   tttAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTg                                                                                                      >  1:215996/1‑78 (MQ=255)
                                                                                                                   tttAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCtt                               >  4:167231/1‑149 (MQ=255)
                                                                                                                      aGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCGGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCtttt                             <  5:207234/148‑1 (MQ=255)
                                                                                                                       gcgcGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTa                            <  1:244847/148‑1 (MQ=255)
                                                                                                                         gcgTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGt                         <  2:38971/149‑1 (MQ=255)
                                                                                                                          cgTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGtt                        <  1:94490/149‑1 (MQ=255)
                                                                                                                           gTACACTTATTGCATTATGCGAACGCCGCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGTAAAGATTTGTGTAGTGTTATGAATGGTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTc                       <  5:229815/149‑1 (MQ=21)
                                                                                                                           gTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGtt                        >  7:40360/1‑148 (MQ=255)
                                                                                                                           gTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTc                       <  2:287177/149‑1 (MQ=255)
                                                                                                                             acacGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTcat                     >  8:213129/1‑147 (MQ=255)
                                                                                                                              cacgtattgcattATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTcat                     <  6:165278/145‑3 (MQ=255)
                                                                                                                               acgtattgcattATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTAt                                                   >  1:389211/3‑117 (MQ=255)
                                                                                                                                   attgcattATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCtttt                             >  7:349625/1‑135 (MQ=255)
                                                                                                                                        attatGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTa                                                    >  3:113614/1‑107 (MQ=255)
                                                                                                                                        attatGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTAt                           <  6:247583/132‑1 (MQ=255)
                                                                                                                                           atGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTaac        <  4:189776/148‑2 (MQ=255)
                                                                                                                                               cAACGCCCCGTGGCTTACCCCGAAGAAACCGGACGTTCTGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGATTTCTCTCATTAAATAGTAATGAATTACCATATGTTTAGTAATATCTTTTATGTTCATGGAGATTTGAAACCCATc   <  4:131238/149‑1 (MQ=255)
                                                                                                                                               cAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTTGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATc   <  1:128675/149‑1 (MQ=255)
                                                                                                                                               cAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATc   <  8:268613/149‑1 (MQ=255)
                                                                                                                                                aaCGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAg                                              <  3:23046/105‑1 (MQ=255)
                                                                                                                                                aaCGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTAt                           <  5:127439/124‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GTATGCCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCG  >  NC_000913/578999‑579291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: