Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	758,061	G→T	G125* (GGA→TGA)	sdhB →	succinate dehydrogenase, FeS subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	758,061	0	G	T	100.0%	168.4 / NA	48	G125* (GGA→TGA)	sdhB	succinate dehydrogenase, FeS subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/23);  total (25/23)

CCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGG  >  NC_000913/757918‑758200                                                                                                                                                |                                                                                                                                            ccccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACa                                                                                                                                         >  5:2840/1‑148 (MQ=255)  cccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACaa                                                                                                                                        >  1:58187/1‑148 (MQ=255)  cccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACaa                                                                                                                                        >  2:157044/1‑148 (MQ=255)   ccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGa                                                                                                                                           <  1:153073/144‑1 (MQ=255)   ccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGa                                                                                                                                           >  2:153073/1‑144 (MQ=255)   ccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACaaaa                                                                                                                                      <  2:58187/149‑1 (MQ=255)           ggCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCg                                                                                                                                  >  1:5165/1‑145 (MQ=255)           ggCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCg                                                                                                                                  <  2:5165/145‑1 (MQ=255)                           ggCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACa                                                                                                              <  1:110277/149‑1 (MQ=255)                                gaagaTTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGc                                                                                                         <  6:2840/149‑1 (MQ=255)                                     ttGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCagag                                                                                                    <  1:157044/149‑1 (MQ=255)                                           ttCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAgcgc                                                                                              <  2:151817/149‑1 (MQ=255)                                                      ccAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTAc                                                                                                               <  6:56021/121‑1 (MQ=255)                                                      ccAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTAc                                                                                                               >  5:56021/1‑121 (MQ=255)                                                          gTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACggg                                                                               >  1:169631/1‑149 (MQ=255)                                                                 ggTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCa                                                                                                       <  7:34276/118‑1 (MQ=255)                                                                 ggTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCa                                                                                                       >  8:34276/1‑118 (MQ=255)                                                                       ccGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTa                                                                  >  6:168685/1‑149 (MQ=255)                                                                        cgcgATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAg                                                                                                             >  3:92400/1‑105 (MQ=255)                                                                        cgcgATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAg                                                                                                             <  4:92400/105‑1 (MQ=255)                                                                         gcgATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTAtt                                                                <  2:169631/149‑1 (MQ=255)                                                                           gATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCt                                                                             >  3:118722/1‑134 (MQ=255)                                                                           gATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCt                                                                             <  4:118722/134‑1 (MQ=255)                                                                                    gtAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGAt                                                                                                           <  2:63687/95‑1 (MQ=255)                                                                                    gtAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGAt                                                                                                           >  1:63687/1‑95 (MQ=255)                                                                                          aTGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATgttgtt                                                >  3:62129/1‑148 (MQ=255)                                                                                            gggACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCaa                                             >  2:119694/1‑149 (MQ=255)                                                                                            gggACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCACCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCAtgt                                                    >  2:120383/1‑142 (MQ=255)                                                                                            gggACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCACCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCAtgt                                                    <  1:120383/142‑1 (MQ=255)                                                                                             ggACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGaaaaa                                                                                        <  7:114370/105‑1 (MQ=255)                                                                                             ggACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGaaaaa                                                                                        >  8:114370/1‑105 (MQ=255)                                                                                             ggACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAAc                                            >  3:138939/1‑149 (MQ=255)                                                                                                 aaTTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTcgc                                                                                                                         <  5:132981/68‑1 (MQ=255)                                                                                                 aaTTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTcgc                                                                                                                         >  6:132981/1‑68 (MQ=255)                                                                                                  aTTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCtt                                       >  1:86828/1‑149 (MQ=255)                                                                                                          cgcAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCt                               >  2:52386/1‑149 (MQ=255)                                                                                                              atatGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCt                           <  1:52386/149‑1 (MQ=255)                                                                                                                 tGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCtggt                        >  1:43959/1‑149 (MQ=255)                                                                                                                  gagaAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTctct                                                            <  4:34160/112‑1 (MQ=255)                                                                                                                  gagaAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTctct                                                            >  3:34160/1‑112 (MQ=255)                                                                                                                   agaAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCtggtg                       <  7:73438/148‑1 (MQ=255)                                                                                                                   agaAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCtggtg                       >  8:73438/1‑148 (MQ=255)                                                                                                                                  aCCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGt         <  1:119694/147‑1 (MQ=255)                                                                                                                                       ttGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAAc                                                                                       >  6:526/1‑64 (MQ=255)                                                                                                                                       ttGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAAc                                                                                       <  5:526/64‑1 (MQ=255)                                                                                                                                       ttGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCgg  <  4:62129/149‑1 (MQ=255)                                                                                                                                         gAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTAt                                                                         <  6:147276/76‑1 (MQ=255)                                                                                                                                         gAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTAt                                                                         >  5:147276/1‑76 (MQ=255)                                                                                                                                                |                                                                                                                                            CCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGG  >  NC_000913/757918‑758200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑