Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,900,088 Δ1 bp 5.2% coding (1498/1557 nt) yoaE ← putative membrane protein/conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,900,0880A. 5.2% 251.0 / 6.0 77coding (1498/1557 nt)yoaEputative membrane protein/conserved protein
Reads supporting (aligned to +/- strand):  ref base A (38/35);  new base . (2/2);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGATATCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCCT  >  NC_000913/1899949‑1900234
                                                                                                                                           |                                                                                                                                                  
ccAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTgg                                                                                                                                            <  5:330389/148‑1 (MQ=255)
   aaTGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCCCGCCCAGTATGCCGTTACGTTAACGCGGACTCATCTGCATCGTGCGCCGGTTGCTCGTTAACAATGCGCACCAGCTGAACACGATAATCATTGGcgt                                                                                                                                         >  5:212649/1‑146 (MQ=255)
     tggctggcGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCa                                                                                                                                       <  8:186146/148‑1 (MQ=255)
          ggcGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTACCAATGCGAACCAGAGCAACACGATAAGCATTGGCTTCAatgat                                                                                                                                  >  5:127727/1‑148 (MQ=255)
            ctggCAGCCAACCGCCGCCCGCCAGGCATAATGTACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTTAGACTTTTGCTATTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATgg                                                                                                                                <  4:128277/146‑1 (MQ=255)
              ggCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCCTTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGATCCGGTTGCTCTTTAACTATTCTAACGAGATCTACACGATAATCATTGGCTTCAATGATGGTg                                                                                                                              <  8:172749/148‑1 (MQ=255)
               gCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTTTTTTTTTTTATTTTTAACTATTCGAACGAGATCTACACGATAATCATTGGCTTCAATGATGGTGa                                                                                                                             <  2:44683/148‑1 (MQ=255)
                      aCCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAg                                                                                                                      >  3:224684/1‑148 (MQ=255)
                           cgcccgccCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGcc                                                                                                                 >  6:394616/1‑148 (MQ=255)
                                   cgGCATAATGGACAAATCACGACCATTATTCCGTTACGTTAACGCTTACTCATCTTCATCTTTCTTTTGTTTCTTTTTACCAATTCGATCTAGATCTACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACatca                                                                                                         <  6:167364/148‑1 (MQ=255)
                                    ggCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACatcaa                                                                                                        <  2:435510/148‑1 (MQ=255)
                                    ggCATAATGGACATATCAAGCCCATTATGCCGTTACGTTTACGCTTACTCATCTTCATCGTGCGCCGGGTGCTCTTTAACAATGCGAAACAGCTCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACatca                                                                                                         >  8:419434/1‑147 (MQ=255)
                                          aTGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACAt                                                                                                  >  7:49025/1‑148 (MQ=255)
                                                atatCACGCACATTATGCCGTTACGTTAACGCTTACTCATCTTCATAGTGCGCCGGTTGCTATTTAACAATACTAAAGAGATCAACACTATAATCATTGGATTCAATGTTGGTTATATGCAGTGGcc                                                                                                                 >  7:307855/1‑127 (MQ=255)
                                                  atCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTAc                                                                                                             <  6:377459/129‑1 (MQ=255)
                                                  atCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTAc                                                                                                             >  5:377459/1‑129 (MQ=255)
                                                  atCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCcaca                                                                                          >  3:49169/1‑148 (MQ=255)
                                                   tCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCacac                                                                                         >  1:113705/1‑148 (MQ=255)
                                                    cACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGc                                                                                                                        >  1:346289/1‑116 (MQ=255)
                                                    cACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGc                                                                                                                        <  2:346289/116‑1 (MQ=255)
                                                    cACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACg                                                                                        >  3:90428/1‑148 (MQ=255)
                                                     aCGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACgg                                                                                       >  8:89798/1‑148 (MQ=255)
                                                      cGCCCAATATCCCGATAAGTTAACGCTTACTCATCTTCATAGTGCGCCGGATGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAAGGATGGTCATATGCAGTGGCCCTACATCAATCACATCGCCCACACggg                                                                                      >  4:123046/1‑148 (MQ=255)
                                                       gCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCaa                                                                                                                                      >  4:216466/1‑99 (MQ=255)
                                                       gCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCaa                                                                                                                                      <  3:216466/99‑1 (MQ=255)
                                                         ccATTCGGCCGGTACGTGCACGCTTACGCCTCTTCCTCGTGCGCCGGTTGCTCTGTAACACTGCGAACCAGATCACCCCGAAACTCCTTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGCGGaa                                                                                   <  6:212649/148‑1 (MQ=255)
                                                         ccATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGGGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGAAGTGGCCCTACATCAATCACATCGCCCACACGGGGaa                                                                                   <  2:193775/148‑1 (MQ=255)
                                                         ccATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGccc                                                                                             <  1:432913/138‑1 (MQ=255)
                                                         ccATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGccc                                                                                             >  2:432913/1‑138 (MQ=255)
                                                             tATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGat                                                                                                                            <  5:407623/103‑1 (MQ=255)
                                                             tATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGat                                                                                                                            >  6:407623/1‑103 (MQ=255)
                                                             tATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAg                                                                                                                      >  5:93933/1‑109 (MQ=255)
                                                             tATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAg                                                                                                                      <  6:93933/109‑1 (MQ=255)
                                                              aTGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAAtgtg                                                                               <  2:113705/147‑1 (MQ=255)
                                                               tGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAatgatg                                                                                                                                 >  1:88239/1‑96 (MQ=255)
                                                               tGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAatgatg                                                                                                                                 <  2:88239/96‑1 (MQ=255)
                                                                gCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGAc                                                                             >  2:135789/1‑147 (MQ=255)
                                                                  cgttacgttaACGCTTACTCATCTTCATCGTGCGCCGCTTGCTCTTTAACAATGCGAACCAGATCAACACGATAAACATTGGCTTCAATGATCATGATATGCAGTGGCCCTAAATCAATCACATGGACCACACAGGGAATGTGACCAt                                                                          >  2:233903/1‑148 (MQ=255)
                                                                     tacgttaACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGTTAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTg                                                                       >  4:101114/1‑148 (MQ=255)
                                                                       cgttaACGCTTACTCATATTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATAAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCACACACGGGGAATGTGACCATTTGcc                                                                     >  1:407873/1‑148 (MQ=255)
                                                                        gttaACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGcc                                                                     >  4:361042/1‑147 (MQ=255)
                                                                         ttaACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGcc                                                                     >  1:199009/1‑146 (MQ=255)
                                                                         ttaACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGcc                                                                     >  8:296917/1‑146 (MQ=255)
                                                                               cTTACTGATCTTCATCGTGCGCCGGTTGCTATTTAACAATGCGAACCAGATCAAGACGTTAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCAc                                                             <  2:407873/148‑1 (MQ=255)
                                                                                  aCTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGt                                                                                                                     <  1:420544/89‑1 (MQ=255)
                                                                                  aCTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGt                                                                                                                     >  2:420544/1‑89 (MQ=255)
                                                                                  aCTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGa                                                           <  4:90428/147‑1 (MQ=255)
                                                                                       tCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCcg                                                     >  2:837/1‑148 (MQ=255)
                                                                                        cTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATcac                                                                                                    >  3:32268/1‑100 (MQ=255)
                                                                                        cTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATcac                                                                                                    <  4:32268/100‑1 (MQ=255)
                                                                                        cTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACGTCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCcgc                                                    >  2:440732/1‑148 (MQ=255)
                                                                                        cTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCcgc                                                    >  3:114882/1‑148 (MQ=255)
                                                                                           cATCGTGCGCCGGTTGCTCTTTAACAATGCGCACAAGATCCACACGATAATCATTGGCTTCAATTATGGTGATATGCAGTTGCCCTACATCAATCACCTCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCcgc                                                    <  1:333400/145‑1 (MQ=255)
                                                                                              cGTGCGCCGGTTGCTCTTTAACACTGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGat                                                                                                                            <  1:396938/70‑1 (MQ=255)
                                                                                              cGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGat                                                                                                                            >  2:396938/1‑70 (MQ=255)
                                                                                              cGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGt                                              >  3:222783/1‑148 (MQ=255)
                                                                                                tGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCCCGAGGCCCGCGACCGTcg                                            <  4:224684/148‑1 (MQ=255)
                                                                                                tGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTcg                                            <  7:296917/148‑1 (MQ=255)
                                                                                                  cgcCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTcgcg                                          <  5:394616/148‑1 (MQ=255)
                                                                                                        ttGCTCTTTAACAATGCGAACCAGATCAACACGATa                                                                                                                                                    <  4:123569/36‑1 (MQ=255)
                                                                                                        ttGCTCTTTAACAATGCGAACCAGATCAACACGATa                                                                                                                                                    >  3:123569/1‑36 (MQ=255)
                                                                                                          gCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGATatcat                                   <  1:135789/147‑1 (MQ=255)
                                                                                                             ctTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACg                                                            >  2:439244/1‑119 (MQ=255)
                                                                                                             ctTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACg                                                            <  1:439244/119‑1 (MQ=255)
                                                                                                              tttAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGATAtcatcgtca                              >  4:243553/1‑148 (MQ=255)
                                                                                                               ttAACAATGCGAACCAGATCAACACGATATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGat                                        <  1:209188/136‑1 (MQ=255)
                                                                                                               ttAACAATGCGAACCAGATCAACACGATATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGat                                        <  1:203012/136‑1 (MQ=255)
                                                                                                               ttAACAATGCGAACCAGATCAACACGATATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGat                                        >  2:203012/1‑136 (MQ=255)
                                                                                                               ttAACAATGCGAACCAGATCAACACGATATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGat                                        >  2:209188/1‑136 (MQ=255)
                                                                                                                tAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACGTCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGATAtcatcgtca                              <  1:440732/146‑1 (MQ=255)
                                                                                                                tAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGATAtcatcgtcat                             <  8:143554/147‑1 (MQ=255)
                                                                                                                      tGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGATATCATCGTCATCGGCAAg                      >  8:400042/1‑148 (MQ=255)
                                                                                                                       gCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGATATCATCGTCATCGGCAAgg                     >  5:363167/1‑148 (MQ=255)
                                                                                                                             cAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGATATCATCGTCATCGGCAAGGTGCTCa               <  2:199009/148‑1 (MQ=255)
                                                                                                                                   aaCACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCACTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGATATCATCGTCATCGGCTAGGTGCTAAACATCa         <  3:361042/148‑1 (MQ=255)
                                                                                                                                    acacGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACg                                                            <  1:310371/96‑1 (MQ=255)
                                                                                                                                    acacGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACg                                                            >  2:310371/1‑96 (MQ=255)
                                                                                                                                       cgttAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGATATCATCGTCATCGGCAAGGTGCTCAACATCAAgcg     <  3:101114/145‑1 (MQ=255)
                                                                                                                                          tAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGATATCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCct  <  1:837/148‑1 (MQ=255)
                                                                                                                                           aaTCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGATATCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCct  <  7:419434/147‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                  
CCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGATATCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCCT  >  NC_000913/1899949‑1900234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: