Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,357,647 Δ1 bp 5.1% coding (971/2148 nt) cadA ← lysine decarboxylase, acid‑inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,357,6470A. 5.1% 396.4 / 11.9 118coding (971/2148 nt)cadAlysine decarboxylase, acid‑inducible
Reads supporting (aligned to +/- strand):  ref base A (51/60);  new base . (3/3);  total (54/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

GAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGCGTGCTGGAATTCACTCTGTG  >  NC_000913/4357500‑4357791
                                                                                                                                                    |                                                                                                                                                
gagaACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTca                                                                                                                                                  <  2:203431/148‑1 (MQ=255)
 agaACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTcac                                                                                                                                                 <  8:343286/148‑1 (MQ=255)
    ccgccgaCAGCAGTTGGGGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCCCCGCTCATACCGCCTTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACAt                                                                                                                                               <  2:91083/146‑1 (MQ=255)
    aCGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATc                                                                                                                                              >  8:250909/1‑148 (MQ=255)
     cgccgcCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATcc                                                                                                                                             <  7:353988/148‑1 (MQ=255)
     cgccgcCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATcc                                                                                                                                             <  8:57631/148‑1 (MQ=255)
         gccAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATGACTTTCCGTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAgtg                                                                                                                                         <  7:126726/148‑1 (MQ=255)
                gTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCtt                                                                                                                                  <  4:88178/148‑1 (MQ=255)
                 tttGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCtt                                                                                                                                  >  1:104208/1‑147 (MQ=255)
                 tttGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtg                                                                                                                                 <  4:375179/148‑1 (MQ=255)
                 tttGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtg                                                                                                                                 <  3:310378/148‑1 (MQ=255)
                   tgtgAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtga                                                                                                                                <  7:250909/147‑1 (MQ=255)
                   tgtgAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtga                                                                                                                                <  2:226814/147‑1 (MQ=255)
                    gtgAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtgat                                                                                                                               >  1:228789/1‑147 (MQ=255)
                     tgAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtgatga                                                                                                                             <  2:104208/148‑1 (MQ=255)
                        gTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtg                                                                                                                                 <  2:115705/141‑1 (MQ=255)
                        gTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtg                                                                                                                                 >  1:115705/1‑141 (MQ=255)
                        gTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGt                                                                                                                          <  5:28455/148‑1 (MQ=255)
                           gACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCg                                                                                                                        <  8:386631/147‑1 (MQ=255)
                             cTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGgtg                                                                                                                     >  1:106262/1‑148 (MQ=255)
                               gggTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGtgtt                                                                                                                   >  6:385928/1‑148 (MQ=255)
                                tgTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGTATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGgtgt                                                                                                                    <  2:34758/145‑1 (MQ=255)
                                ggTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGgtgt                                                                                                                    <  8:331116/146‑1 (MQ=255)
                                  tttCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTca                                                                                                                                                  >  4:286767/1‑114 (MQ=255)
                                  tttCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTca                                                                                                                                                  <  3:286767/114‑1 (MQ=255)
                                    tCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTa                                                                                                                >  1:436860/1‑146 (MQ=255)
                                    tCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTAc                                                                                                               <  6:324583/147‑1 (MQ=255)
                                      gTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTAcag                                                                                                             <  8:148273/147‑1 (MQ=255)
                                        aaaTCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTAcag                                                                                                             >  5:178144/1‑145 (MQ=255)
                                          aTCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCAAGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGAc                                                                                                        >  7:3589/1‑148 (MQ=255)
                                              cTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcat                                                                                                     >  4:320907/1‑147 (MQ=255)
                                                  cccTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAg                                                                                                >  3:44481/1‑148 (MQ=255)
                                                      tCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCgg                                                                                                                       <  3:297752/121‑1 (MQ=255)
                                                      tCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGgtgt                                                                                                                    <  7:213000/124‑1 (MQ=255)
                                                      tCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGgtgt                                                                                                                    >  8:213000/1‑124 (MQ=255)
                                                       cTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtgatg                                                                                                                              >  1:164656/1‑113 (MQ=255)
                                                            cGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTgg                                                                                      <  2:228789/148‑1 (MQ=255)
                                                            cGGCCACCGCTCAAACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATATCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTgg                                                                                      >  2:57290/1‑148 (MQ=255)
                                                             ggCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTAcagc                                                                                                            <  7:93584/125‑1 (MQ=255)
                                                             ggCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTAcagc                                                                                                            >  8:87430/1‑125 (MQ=255)
                                                             ggCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTAcagc                                                                                                            <  7:87430/125‑1 (MQ=255)
                                                              gCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTa                                                                                    <  5:385928/148‑1 (MQ=255)
                                                                   cGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTa                                                                                >  7:369860/1‑147 (MQ=255)
                                                                    gCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTaga                                                                                           <  7:389604/135‑1 (MQ=255)
                                                                    gCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTaga                                                                                           >  8:389604/1‑135 (MQ=255)
                                                                      tCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGCAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAg                                                                             >  5:6930/1‑147 (MQ=255)
                                                                          aCCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATg                                                                         >  7:119263/1‑147 (MQ=255)
                                                                           ccGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTa                                                                       <  6:117201/148‑1 (MQ=255)
                                                                            cGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTAc                                                                      <  2:106262/148‑1 (MQ=255)
                                                                            cGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTAc                                                                      >  4:337009/1‑148 (MQ=255)
                                                                               aTTTACCTTCGTAAATCGGTGAGAAGTTGGTGTATGGCAACCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGGAGAGTTGGTAATTACAGCATGTACCgg                                                                   <  4:132273/148‑1 (MQ=255)
                                                                                 ttACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGc                                                                  >  7:218271/1‑147 (MQ=255)
                                                                                 ttACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGc                                                                  >  3:363402/1‑147 (MQ=255)
                                                                                   aactctacctatgatggtctgctgtacaacaccgacttcatcaagaaaacacTGGATGTGAAATCCATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAg                                                               <  8:269442‑M1/96‑1 (MQ=255)
                                                                                   aactctacctatgatggtctgctgtacaacaccgacttcatcaagaaaacacTGGATGTGAAATCCATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAg                                                               <  4:250656‑M1/96‑1 (MQ=255)
                                                                                   aCCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAAtt                                                                                 >  1:421149/1‑130 (MQ=255)
                                                                                      ttCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCa                                                                <  2:347254/144‑1 (MQ=255)
                                                                                      ttCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCa                                                                >  1:347254/1‑144 (MQ=255)
                                                                                       tCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGt                                                                                              <  6:301214/113‑1 (MQ=255)
                                                                                       tCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTg                                                           >  2:202431/1‑148 (MQ=255)
                                                                                         gTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCg                                                         >  8:295096/1‑148 (MQ=255)
                                                                                            aaTCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGgtt                                                      >  7:437180/1‑148 (MQ=255)
                                                                                                  tGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGtt                                                <  7:295096/148‑1 (MQ=255)
                                                                                                   gtctgctgtacaacaccgacttcatcaagaaaacacTGGATGTGAAATCCATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGttt                                               >  7:269442‑M1/37‑148 (MQ=255)
                                                                                                   gagaAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcatcat                                                                                                  >  8:291418/1‑97 (MQ=255)
                                                                                                   gagaAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcatcat                                                                                                  <  7:291418/97‑1 (MQ=255)
                                                                                                   gagaAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGttt                                                      >  8:85069/1‑141 (MQ=255)
                                                                                                   gagaAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGttt                                                      <  7:85069/141‑1 (MQ=255)
                                                                                                    agaAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGtttc                                              <  3:403786/148‑1 (MQ=255)
                                                                                                       aGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGtttcttt                                           <  3:102433/148‑1 (MQ=255)
                                                                                                         ttGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCa                                         <  4:44481/148‑1 (MQ=255)
                                                                                                         ttGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCa                                         <  8:369860/148‑1 (MQ=255)
                                                                                                          tGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcatc                                                                                                    <  4:257202/88‑1 (MQ=255)
                                                                                                          tGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcatc                                                                                                    >  3:257202/1‑88 (MQ=255)
                                                                                                          tGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcatc                                                                                                    >  3:176572/1‑88 (MQ=255)
                                                                                                          tGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCAc                                        >  6:15066/1‑148 (MQ=255)
                                                                                                            gtgtAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCAcgc                                      >  3:280213/1‑148 (MQ=255)
                                                                                                               tAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACgcgc                                    <  4:280213/147‑1 (MQ=255)
                                                                                                                aaGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGCTTCTAATTACAGCATGTACCGGCCAGTGTGCGTTTGGTGTTTCATTCACGCGCt                                   <  8:3589/147‑1 (MQ=255)
                                                                                                                aaGGCACCCACGCGGAGTAAAAGTGGATGGATTTCACAACCACTGTTTTCATGATGAAGTCGTTGTTGTACATCAGACCATCATAGGTATAGTTGGTAATTACAGCATATACCGGACAGGTTGCGTTAGGTCATTATTTCAcgcaat                                   >  6:401072/1‑144 (MQ=255)
                                                                                                                 aGGCTCCCACGCGGAGTCAAAGGGGATGGATTTCACATGCAGTGTTTTCTTGATGAATTCGGTGTTGTACCGCAGACCTTCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCtt                                  <  3:320907/147‑1 (MQ=255)
                                                                                                                     aCCCACGCGGAGTCAAAGAGGCTGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCaa                             <  6:93233/148‑1 (MQ=255)
                                                                                                                       ccACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATg                           >  4:332553/1‑148 (MQ=255)
                                                                                                                       ccACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATg                           <  1:202431/148‑1 (MQ=255)
                                                                                                                        cACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATgg                          <  2:436860/148‑1 (MQ=255)
                                                                                                                        cACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCAGAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCAGGCTCTTAGGAATgg                          >  2:316916/1‑148 (MQ=255)
                                                                                                                         aCGCGGAGTCAACGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGGTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGt                         <  1:110348/148‑1 (MQ=255)
                                                                                                                           gcgGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtgatga                                                                                                                             >  8:287555/1‑46 (MQ=255)
                                                                                                                           gcgGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtgatga                                                                                                                             <  7:287555/46‑1 (MQ=255)
                                                                                                                            cgGAGTCAAAGTGGATGGATTTCAGATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCCCGCGCTTAGCAATGGTAGc                      >  1:133700/1‑148 (MQ=255)
                                                                                                                              gAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcatc                                                                                                    >  3:397423/1‑68 (MQ=255)
                                                                                                                               aGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGCGTg                   <  8:84105/148‑1 (MQ=255)
                                                                                                                                  cAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcatc                                                                                                    >  6:158775/1‑64 (MQ=255)
                                                                                                                                  cAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcatc                                                                                                    <  5:158775/64‑1 (MQ=255)
                                                                                                                                  cAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCAAAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGCGTGCTg                >  3:406950/1‑148 (MQ=255)
                                                                                                                                    aaGTGTATGGATTTGTCATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTAGATCTTACAATCATAGGTTGAGTTGGTATTTACAGTATGTAACGTCCAGGTTTCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGAGTGCTGGa              <  2:408323/148‑1 (MQ=255)
                                                                                                                                        GGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGCGTGCTGGAAtt           <  7:415833‑M1/147‑1 (MQ=255)
                                                                                                                                          ATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGCGTGCTGGAATTCAc        >  5:37048‑M1/1‑148 (MQ=255)
                                                                                                                                              gatTTCACATGCCGTGTTTTCTTGATGAAGTCGGTGTTGTACTGCAGACCATCATAGGTAGACTTGGGATTTACAGCATGTACCGCCCAGGTTGCGTTTGGTGATTCTTTCACGCGCTTAGCAATGGTAGCGCGCTGGAATTCActct     <  8:218271/148‑1 (MQ=255)
                                                                                                                                                  tCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGCGTGCTGGAATTCACTCtgtg  <  3:332553/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGCGTGCTGGAATTCACTCTGTG  >  NC_000913/4357500‑4357791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: