| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,221,641 | T→A | 7.4% | pseudogene (2041/2574 nt) | ycgH → | pseudogene; putative ATP‑binding component of a transport system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,221,641 | 0 | T | A | 7.4% | 165.2 / 4.2 | 54 | pseudogene (2041/2574 nt) | ycgH | pseudogene; putative ATP‑binding component of a transport system |
| Reads supporting (aligned to +/- strand): ref base T (29/21); new base A (2/2); total (31/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01 | |||||||||||
GGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAAAAACTCGGTGAAAGGGGATGAATTAGCCTATGAATCCTATAGCGCGAAAGGTGCAACCGTCTCGCTGGAAG > NC_000913/1221494‑1221788 | gtcGATATGTTTTCGGGTGGTTTTACGGATAGTGACAGTTGTCGTTTGGGAGTGATGGCTGGTTTTGCCAGCGACTACAACTTAACTCATTCGAGCGTGTCGGATTATCGTTCGATAGTGATTGTCAGAGGCTATAGCGCAGGGCTGt < 7:443392/146‑1 (MQ=255)ggCGATCTGTTACCCGGTGGGTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGt < 7:358961/148‑1 (MQ=255)ggCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGt < 2:77291/148‑1 (MQ=255)ggCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGt < 7:177457/148‑1 (MQ=255) cGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTGGGGAGTGATGGCTGGTTATGCCCGCGACAACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTAt < 2:280966/148‑1 (MQ=255) ttAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTGGGGAGTGATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCGGTATGcc < 5:294150/144‑1 (MQ=255) tAACCGGTGGTTTTACCGATAGTGCCAGTTGGCGTTTGGGAGTGATGGCTGGTTATGCACGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTg > 8:400539/1‑148 (MQ=255) ggtggtTGTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGt < 5:391823/133‑1 (MQ=255) gtggtTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGAGTATCGTTCGAAAGGGAGTGTCAGAGGCTAAAGCGCAGGGGTGTa > 6:391823/1‑133 (MQ=255) gtTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGa < 3:198757/148‑1 (MQ=255) gACAGTTGGCGTTTGGGAGTGATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGACTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTaaa > 3:88405/1‑147 (MQ=255) ttGGCGTTTGGGAGTGATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGt > 2:121743/1‑110 (MQ=255) ttGGCGTTTGGGAGTGATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGt < 1:121743/110‑1 (MQ=255) gTTTGGGAGTGATGGCGGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTACCGATGACATCAGTAAAAAAGGCGCATa < 5:14206/148‑1 (MQ=255) ttGGGAGTGATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGt > 4:155658/1‑103 (MQ=255) ttGGGAGTGATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGt < 3:155658/103‑1 (MQ=255) ggAGTGATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCTTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTg < 1:244379/148‑1 (MQ=255) aGTGATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGAc > 7:344481/1‑148 (MQ=255) gATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTg < 1:223539/143‑1 (MQ=255) gATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTg > 2:223539/1‑143 (MQ=255) gATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTcc > 5:315243/1‑148 (MQ=255) tGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTccgg > 5:51720/1‑146 (MQ=255) tGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCt < 3:48388/147‑1 (MQ=255) ggTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAgg < 1:214275/125‑1 (MQ=255) ggTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAgg > 2:214275/1‑125 (MQ=255) tGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAata < 4:88405/147‑1 (MQ=255) cccGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAg > 2:3980/1‑148 (MQ=255) tACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGttt > 7:279717/1‑147 (MQ=255) aCAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGt < 1:204158/72‑1 (MQ=255) aCAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGt > 2:204158/1‑72 (MQ=255) aCAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTa > 5:41276/1‑147 (MQ=255) aCTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTaaaaa > 1:116770/1‑148 (MQ=255) aCTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTaaaa > 2:153498/1‑147 (MQ=255) aCTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTaaaa < 1:153498/147‑1 (MQ=255) aaCTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAAAAACTc < 3:248124/147‑1 (MQ=255) aCTCATTCCAGCTTGGCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGTCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAAAAACTCgg < 2:320527/148‑1 (MQ=255) aCTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAAAAACTCg < 6:315243/147‑1 (MQ=255) tCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAAAAACTCGGt > 1:297005/1‑147 (MQ=255) cATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAAAAACTCGGTg > 8:57796/1‑147 (MQ=255) cATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAAAAACTCGGTg > 4:55266/1‑147 (MQ=255) aTTCCAGCTGGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTTGGCGCAATATAGCTGGTTTAAAAACTCGGTGaa < 1:52066/148‑1 (MQ=255) ttCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGAATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCAt > 8:126571/1‑101 (MQ=255) ttCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGAATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCAt > 8:132878/1‑101 (MQ=255) ttCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGAATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCAt < 7:132878/101‑1 (MQ=255) ttCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGAATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCAt < 7:126571/101‑1 (MQ=255) tCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAAAAACTCGGTGAAAg > 7:368001/1‑148 (MQ=255) tCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGCTCTGTATTCCCCCTGGTTTGCCGATGACATCAGTAAAAAAGGAGCATACATTGACTCCTTGGCGCAATAAAGAGGGTTTAAAAACACCGTGAAAg > 5:140013/1‑148 (MQ=255) ccAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAAAAACTCGGTGAAAg < 2:297005/147‑1 (MQ=255) tgtCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAAAAACTCGGTGAAAGGGGATGa < 7:400539/148‑1 (MQ=255) cGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGt < 1:115899/36‑1 (MQ=255) cGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGt > 2:115899/1‑36 (MQ=255) aaaGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAAAAACTCGGTGAAAGGGGATGAATTAGCCTATGAATCCt < 2:116770/148‑1 (MQ=255) aGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGG‑TTGCCGATGACATCAGTAAAAAAGgcgc > 3:371348/1‑71 (MQ=255) aGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGG‑TTGCCGATGACATCAGTAAAAAAGgcgc < 4:371348/71‑1 (MQ=255) gtCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGgcgc > 1:175072/1‑65 (MQ=255) gtCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGgcgc < 2:175072/65‑1 (MQ=255) gtCAGACGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCAGGTTTAAAAACTCGGTGAAAGTGGATGAATTAGACTATGAATCCTAAAgcgag > 2:89229/1‑145 (MQ=255) ataGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAAGATAGCTGGTTTAAAAACTCGGTGAAAGGGGATGAATTAGCCTATGAATCCTATAGCGCGAAAGGTGCAAc > 6:316537/1‑148 (MQ=255) taGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAAtata > 1:322633/1‑80 (MQ=255) taGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAAtata < 2:322633/80‑1 (MQ=255) aGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAAAAACTCGGTGAAAGGGGATGAATTAGCCTATGAATCCTATAGCGCGAAAGGTGCAACCg > 7:7220/1‑148 (MQ=255) aGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAAAAACTCGGTGAAAGGGGATGAATTAGCCTATGAATCCTATAGCGCGAAAGGTGCAACCg > 4:359517/1‑148 (MQ=255) gcAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAAAAACTCGGTGAAAGGGGATGAATTAGCCTATGAATCCTATAGCGCGAAAGGTGCAACCGtct > 5:14729/1‑148 (MQ=255) aGGTCTGTATGACACCTGGTTTTCCGATGACATCAGTAAAAAATGCTCATTCATTGACTACTGGGCGCAATATAGCTGGTTTATATACTCGGTGAATGGGGATTAATTATCCTATGAATCCTATAGCGCGAAAGGTGCAACGGTCTCg < 6:140013/148‑1 (MQ=255) gCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAAAAACTCGGTGAAAGGGGATGAATTATCCTATGAATCCTATAGCGCGAAAGGTGCAACCGTCTCGCTg > 6:163120/1‑148 (MQ=255) gTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAAAAACTCGGTGAAAGGGGATGAATTAGCCTATGAATCCTATAGCGCGAAAGGTGCAACCGTCTCGCTgg > 6:379751/1‑146 (MQ=255) gTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCCCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAATAACTCGGTGAAAGGGGATGAATTAGCCTATGAATCCTATAGCGCGAAAGGTGCAACCGTCTCTCTgg > 3:57436/1‑146 (MQ=255) tATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGGTTAAAAACTCGGTGAAAGGGGATGAATTAGCCTATGAATCCTATAGCGCGAAAGGTGCAACCGTCTCGCTGGAAg < 7:403968/148‑1 (MQ=255) | GGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGGTTATGCCCGCGACTACAACTTAACTCATTCCAGCGTGTCGGATTATCGTTCGAAAGGGAGTGTCAGAGGCTATAGCGCAGGGCTGTATGCCACCTGGTTTGCCGATGACATCAGTAAAAAAGGCGCATACATTGACTCCTGGGCGCAATATAGCTGGTTTAAAAACTCGGTGAAAGGGGATGAATTAGCCTATGAATCCTATAGCGCGAAAGGTGCAACCGTCTCGCTGGAAG > NC_000913/1221494‑1221788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |