Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,715,919 T→G 5.6% intergenic (‑30/+29) pdxY ← / ← tyrS pyridoxamine kinase/tyrosyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,715,9190TG 5.6% 241.9 / 2.7 77intergenic (‑30/+29)pdxY/tyrSpyridoxamine kinase/tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (39/33);  new base G (3/2);  total (42/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGTTAA  >  NC_000913/1715776‑1716066
                                                                                                                                               |                                                                                                                                                   
ggTGTTCAGCGGCCAGACGTGCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTtct                                                                                                                                                 <  5:240652/148‑1 (MQ=255)
 gtgtTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGGAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTtctc                                                                                                                                                <  6:95603/148‑1 (MQ=255)
 gtgtTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGCCCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTtctc                                                                                                                                                <  5:23524/148‑1 (MQ=255)
 gtgtTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTtctc                                                                                                                                                <  4:101142/148‑1 (MQ=255)
 gtgtTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTtctc                                                                                                                                                <  6:382805/148‑1 (MQ=255)
         cGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTccccc                                                                                                                                        <  5:343367/148‑1 (MQ=255)
              aGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTcc                                                                                                                                   <  6:35051/148‑1 (MQ=255)
               gACGTTCGCGCCCAGGCGGCGCATCGGATACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCGGTATAAAAACAAGGGGGAGTGAGTTCTCCCTCACCCTTTcaa                                                                                                                                  >  6:379234/1‑146 (MQ=255)
               gACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCa                                                                                                                                  >  3:343876/1‑148 (MQ=255)
                  gctcccgcCCCGGCGCCGCATCGGAAACTCTGCCCCACTGTTACCCGCATCACCATAACCCACGTGAGACTGGACAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGAGAAATCACTcccc                                                                                                                                         <  2:317009/133‑1 (MQ=255)
                   ttCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTa                                                                                                                              <  1:125242/148‑1 (MQ=255)
                   ttCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGAGAAATCACTccccc                                                                                                                                        >  1:317009/1‑138 (MQ=255)
                   ttCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAAAATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACtt                                                                                                                               >  2:352345/1‑147 (MQ=255)
                          ccAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCatt                                                                                                                       <  4:365304/148‑1 (MQ=255)
                           cAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCatta                                                                                                                      >  1:68680/1‑148 (MQ=255)
                                ggcgCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTcc                                                                                                                 <  7:423509/148‑1 (MQ=255)
                                        ggAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGAttt                                                                                                                                                   <  8:333156/106‑1 (MQ=255)
                                        ggAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGAttt                                                                                                                                                   >  7:333156/1‑106 (MQ=255)
                                          aaaCTCTGCCGCACTGTTACCCGCATGTCCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAAt                                                                                                          >  7:43785/1‑145 (MQ=255)
                                            aCTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGAc                                                                                                     <  5:395505/148‑1 (MQ=255)
                                               ctGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAg                                                                                                   >  6:179129/1‑147 (MQ=255)
                                                 tccGCCCTTTTACCCGCATTCCCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTGCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGt                                                                                                  <  4:195522/145‑1 (MQ=255)
                                                     cACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCt                                                                                            >  1:195437/1‑148 (MQ=255)
                                                     cACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCt                                                                                            <  8:195807/148‑1 (MQ=255)
                                                      aCTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCtt                                                                                           <  7:281545/148‑1 (MQ=255)
                                                      aCTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCtt                                                                                           <  5:179129/148‑1 (MQ=255)
                                                           tACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTAc                                                                                      >  1:205330/1‑148 (MQ=255)
                                                                 cATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGAc                                                                                >  7:68368/1‑148 (MQ=255)
                                                                        aTAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGt                                                                           >  4:404627/1‑146 (MQ=255)
                                                                          aaaCAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAgg                                                                       >  4:210396/1‑148 (MQ=255)
                                                                          aaaCAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAgg                                                                       >  4:340109/1‑148 (MQ=255)
                                                                            acaacGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGt                                                                      >  2:42323/1‑147 (MQ=255)
                                                                               acGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAACCCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGAATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTaaac                                                                  >  7:306758/1‑147 (MQ=255)
                                                                               acGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTaaaa                                                                  >  2:393587/1‑148 (MQ=255)
                                                                                  tGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGa                                                               >  8:198422/1‑148 (MQ=255)
                                                                                      aCTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCa                                                            >  2:413987/1‑147 (MQ=255)
                                                                                          gATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCaaa                                                          >  6:145989/1‑145 (MQ=255)
                                                                                           aTAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCGCTTTCCCCTTAATGCTTGATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGCCGCTGTAAGGTAAAACGACCAACCAGa                                                      >  1:103271/1‑148 (MQ=255)
                                                                                            tAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGCCGCAGTAAGGTAAAACGACCAAACAGAc                                                     >  6:292116/1‑148 (MQ=255)
                                                                                               cGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACcgcg                                                                                  >  4:72281/1‑116 (MQ=255)
                                                                                               cGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACcgcg                                                                                  <  3:72281/116‑1 (MQ=255)
                                                                                                      atTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtcttcttc                                           >  7:128429/1‑148 (MQ=255)
                                                                                                       tttttCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtcttcttc                                           <  8:43785/147‑1 (MQ=255)
                                                                                                       tttttCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtcttcttc                                           <  1:206267/147‑1 (MQ=255)
                                                                                                         tttCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGa                                                               >  8:191274/1‑125 (MQ=255)
                                                                                                         tttCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGa                                                               <  7:191274/125‑1 (MQ=255)
                                                                                                         tttCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATcttcttctt                                         <  3:365846/147‑1 (MQ=255)
                                                                                                            catcatGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTaa                                      <  3:310002/147‑1 (MQ=255)
                                                                                                                 tGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGt                                <  2:195437/148‑1 (MQ=255)
                                                                                                                  gTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtcttctt                                            <  5:215453/135‑1 (MQ=255)
                                                                                                                  gTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtcttctt                                            >  6:215453/1‑135 (MQ=255)
                                                                                                                   ttCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCtt                                                                                                                                      <  8:186647/44‑1 (MQ=255)
                                                                                                                   ttCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCtt                                                                                                                                      >  7:186647/1‑44 (MQ=255)
                                                                                                                      ccTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTc                            <  1:393587/147‑1 (MQ=255)
                                                                                                                       cTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAg                          >  1:132895/1‑148 (MQ=255)
                                                                                                                       cTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAg                          >  2:414008/1‑148 (MQ=255)
                                                                                                                         gTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGa                        >  1:161125/1‑148 (MQ=255)
                                                                                                                         gTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGa                        >  3:215302/1‑148 (MQ=255)
                                                                                                                          tataAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGAt                       >  7:353401/1‑148 (MQ=255)
                                                                                                                          tataAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGAt                       >  7:347287/1‑148 (MQ=255)
                                                                                                                          tataAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGAt                       >  3:272292/1‑148 (MQ=255)
                                                                                                                              aaaaCCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGa                   >  2:240216/1‑148 (MQ=255)
                                                                                                                                aaCCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACt                 >  7:221228/1‑148 (MQ=255)
                                                                                                                                   cAGGGTGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATATCCAGCAAATCAGACAGTAATTCTATTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGtt              <  5:379234/148‑1 (MQ=255)
                                                                                                                                   aaGGGGGAGTTATTTCTCACTCCCCCTTTGCAATTAATGCATTATTTCCAGCAAATCAGACCGTATTTCTTTTTACCGCGACGCATTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGtt              <  5:99869/147‑1 (MQ=255)
                                                                                                                                    aGGGGGAGTGAGAAATCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTc                            <  5:379386/133‑1 (MQ=255)
                                                                                                                                    aGGGGGAGTGAGAAATCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTc                            >  6:379386/1‑133 (MQ=255)
                                                                                                                                     gggggAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGtttt            <  5:362444/148‑1 (MQ=255)
                                                                                                                                      ggggAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGtttt            >  3:345152/1‑147 (MQ=255)
                                                                                                                                         gAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCAc        >  7:438500/1‑148 (MQ=255)
                                                                                                                                          aGTGATTTCTCACTCCCCCTTTCCACTTAATGCATAATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGAGAAGAAGTAACGTAAAACGACCAAACAGACGATCATCTTCTTTAAAGAAGTATTCAGGATCAGACTGTATTTCAcc       >  8:376804/1‑148 (MQ=255)
                                                                                                                                           gTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCg      <  8:128429/148‑1 (MQ=255)
                                                                                                                                             gATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCaaa                                                          <  3:58930/94‑1 (MQ=255)
                                                                                                                                             gATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCaaa                                                          >  4:58930/1‑94 (MQ=255)
                                                                                                                                             gATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCg                     >  6:103051/1‑131 (MQ=255)
                                                                                                                                             gATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCg                     <  5:103051/131‑1 (MQ=255)
                                                                                                                                             gATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGt     >  3:284021/1‑147 (MQ=255)
                                                                                                                                              aTTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGTTa   <  3:340109/148‑1 (MQ=255)
                                                                                                                                              aTTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGTTa   <  4:215302/148‑1 (MQ=255)
                                                                                                                                               tttCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGTTaa  <  2:330106/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGTTAA  >  NC_000913/1715776‑1716066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: