Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,855,344 T→A 7.9% N210Y (AAT→TAT)  ydjG ← methylglyoxal reductase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,855,3440TA 7.9% 148.2 / 4.2 51N210Y (AAT→TAT) ydjGmethylglyoxal reductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (19/28);  new base A (2/2);  total (21/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

CACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTC  >  NC_000913/1855213‑1855489
                                                                                                                                   |                                                                                                                                                 
caCTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTgg                                                                                                                                    >  8:190342/1‑147 (MQ=255)
caCTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTgg                                                                                                                                    >  1:443042/1‑147 (MQ=255)
 aCTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGgca                                                                                                                                  <  5:27701/148‑1 (MQ=255)
 aCTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGgca                                                                                                                                  <  1:327767/148‑1 (MQ=255)
   tttCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGgcag                                                                                                                                 >  3:70027/1‑147 (MQ=255)
          aTGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGt                                                                                                                         <  7:223107/148‑1 (MQ=255)
             ttttCACGCTGGCACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATGACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACGTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGtttt                                                                                                                      <  8:364101/148‑1 (MQ=255)
             ttttCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGttt                                                                                                                       <  1:304210/147‑1 (MQ=255)
              tttCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATAATCACGACATAGTGGCAGCAGTTCGTTTTc                                                                                                                     >  7:189588/1‑148 (MQ=255)
                tCACGCTGGAACCAGACTTTATTTGCCCGTGCGCCGCCCGGAACGTAATCACGAGTGATGGGGCCGGTCAACAATCCCTGCGCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTcc                                                                                                                    <  6:259397/147‑1 (MQ=255)
                tCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTcc                                                                                                                    <  5:66660/147‑1 (MQ=255)
                         aaCCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGt                                                                                                          >  3:307541/1‑148 (MQ=255)
                              gACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAgg                                                                                                     <  7:282912/148‑1 (MQ=255)
                                 tttATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTg                                                                                                                                     >  6:46996/1‑113 (MQ=255)
                                 tttATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTg                                                                                                                                     <  5:46996/113‑1 (MQ=255)
                                      ttGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATAATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCg                                                                                                        >  7:30955/1‑137 (MQ=255)
                                      ttGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATAATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCg                                                                                                        <  8:30955/137‑1 (MQ=255)
                                             aGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAg                                                                                                                                       >  7:291003/1‑99 (MQ=255)
                                             aGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAg                                                                                                                                       <  8:291003/99‑1 (MQ=255)
                                             aGCGCCGCCCGGAACGTAATCACGAGGGACGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCTGTCGAGGATACTGTATTTCGcc                                                                                      <  5:350665/148‑1 (MQ=255)
                                                     ccGGAACGTAATCACGCGGGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGaataat                                                                              <  2:372424/148‑1 (MQ=255)
                                                      cGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAataata                                                                             <  8:170444/148‑1 (MQ=255)
                                                       ggAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAatat                                                                            <  4:70027/148‑1 (MQ=255)
                                                             tAATCACGAGTGATGGTGCCGGTCAACAATCCCAGCTCTAGCGGGTAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGt                                                                       >  6:300742/1‑147 (MQ=255)
                                                                tCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCAc                                                                   <  7:190342/148‑1 (MQ=255)
                                                                   cGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCa                                                                 >  3:15269/1‑147 (MQ=255)
                                                                   cGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCa                                                                 >  4:350612/1‑147 (MQ=255)
                                                                   cGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATAATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCat                                                                <  8:189588/148‑1 (MQ=255)
                                                                    gAGTGATGATGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCata                                                               <  2:15210/148‑1 (MQ=255)
                                                                      gTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAataata                                                                             <  1:388067/132‑1 (MQ=255)
                                                                      gTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAataata                                                                             >  2:388067/1‑132 (MQ=255)
                                                                        gATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTg                                                            >  2:436114/1‑147 (MQ=255)
                                                                          tGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTg                                                            >  5:388546/1‑145 (MQ=255)
                                                                          tGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAg                                                         <  4:307541/148‑1 (MQ=255)
                                                                          tGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAg                                                         <  1:436114/148‑1 (MQ=255)
                                                                                    caacaaTCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTcgcg                                                >  1:203407/1‑147 (MQ=255)
                                                                                      acaaTCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGat                                             <  3:350612/148‑1 (MQ=255)
                                                                                      acaaTCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGat                                             <  3:348480/148‑1 (MQ=255)
                                                                                        aaTCCCTGCTCTATCGGGGAATAAACCTGAAACACAATGCCATTATCACGACATAGTTGCAGCAGTTCGTTTTCCATTTCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGTatat                                           <  8:97141/148‑1 (MQ=255)
                                                                                                    aGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGa                                                                                   >  4:14184/1‑96 (MQ=255)
                                                                                                    aGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGa                                                                                   <  3:14184/96‑1 (MQ=255)
                                                                                                        gggAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGtt                           <  4:199183/148‑1 (MQ=255)
                                                                                                              aaaCCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGa                                                                                   >  7:297631/1‑86 (MQ=255)
                                                                                                              aaaCCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGa                                                                                   <  8:297631/86‑1 (MQ=255)
                                                                                                                    gAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCta                >  8:369814/1‑147 (MQ=255)
                                                                                                                      aCCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCg                                                                                                        >  6:298563/1‑57 (MQ=255)
                                                                                                                      aCCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCg                                                                                                        <  5:298563/57‑1 (MQ=255)
                                                                                                                              gCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATGTGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAAtt     >  7:134208/1‑148 (MQ=255)
                                                                                                                               ccATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAAttt    <  2:203407/148‑1 (MQ=255)
                                                                                                                                cATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAAtttt   <  7:369814/148‑1 (MQ=255)
                                                                                                                                 aTTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTc  >  1:42052/1‑148 (MQ=255)
                                                                                                                                   tATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAg                                                         >  4:144345/1‑91 (MQ=255)
                                                                                                                                   tATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAg                                                         <  3:144345/91‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                                 
CACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTC  >  NC_000913/1855213‑1855489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑