Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,929,318 T→C 5.1% Q130Q (CAA→CAG yebE ← DUF533 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,929,3180TC 5.1% 247.0 / 3.3 78Q130Q (CAA→CAGyebEDUF533 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (41/33);  new base C (2/2);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

AATAGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGTTCGTCCTGATGCGCCGCG  >  NC_000913/1929174‑1929462
                                                                                                                                                |                                                                                                                                                
aataGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTg                                                                                                                                                <  3:410628/147‑1 (MQ=255)
 ataGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCt                                                                                                                                              >  4:403252/1‑148 (MQ=255)
 ataGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGATTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCt                                                                                                                                              >  2:387066/1‑148 (MQ=255)
        cgcAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGAt                                                                                                                                       <  7:175411/148‑1 (MQ=255)
        cgcAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGAt                                                                                                                                       <  1:367366/148‑1 (MQ=255)
           aaCTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGc                                                                                                                                    <  1:351109/148‑1 (MQ=255)
                   aaaTAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGAtt                                                                                                                                      >  5:361558/1‑138 (MQ=255)
                   aaaTAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGAtt                                                                                                                                      <  6:361558/138‑1 (MQ=255)
                   aaaTAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGtt                                                                                                                            >  3:328582/1‑148 (MQ=255)
                         aTTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTgg                                                                                                                      <  3:323781/148‑1 (MQ=255)
                                       ttcttcATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCa                                                                                                        >  7:89049/1‑148 (MQ=255)
                                        tcttcATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCAt                                                                                                       >  2:103717/1‑148 (MQ=255)
                                          ttcATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGCCCATCa                                                                                                     >  5:192947/1‑148 (MQ=255)
                                             aTTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACtt                                                                                                  <  7:270543/148‑1 (MQ=255)
                                              ttGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCTATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTCTTCGATTGCCGCACGTTCTTTGGCATCAATATGACCAACACttt                                                                                                 >  6:161471/1‑148 (MQ=255)
                                                gCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACtttt                                                                                                <  5:116142/147‑1 (MQ=255)
                                                       ccGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCa                                                                                         >  3:161752/1‑147 (MQ=255)
                                                          gTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGa                                                                                     <  1:308796/148‑1 (MQ=255)
                                                          gTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGa                                                                                     <  1:103717/148‑1 (MQ=255)
                                                            aGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGAcc                                                                                   <  5:315846/148‑1 (MQ=255)
                                                              ccAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCt                                                                                                                                              <  8:116027/87‑1 (MQ=255)
                                                              ccAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCt                                                                                                                                              >  7:116027/1‑87 (MQ=255)
                                                                 ggCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCCCGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGAcc                                                                                   >  1:141210/1‑143 (MQ=255)
                                                                 ggCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAgcg                                                                               >  2:179202/1‑147 (MQ=255)
                                                                 ggCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTGCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGAcc                                                                                   <  2:141210/143‑1 (MQ=255)
                                                                     ttGGGGATCCAGCGGTTGGTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGGCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAgg                                                                          <  7:219533/148‑1 (MQ=255)
                                                                      tgtgGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGa                                                                         >  7:105961/1‑148 (MQ=255)
                                                                           aTCCAGCGGTTGTTCGATAGCCTGCTCAATGAGTACACGCCCCCCCTCTTCCCCGCCGGCTCCACCCAATTGATGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCAATTTTAGCGGCAAAGACCAGCGCGAGGATCaaa                                                                    <  4:328582/148‑1 (MQ=255)
                                                                               aGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTg                                                                >  2:376359/1‑148 (MQ=255)
                                                                                gCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGc                                                               <  3:403252/148‑1 (MQ=255)
                                                                                   gtggttCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGt                                                            <  2:411864/145‑1 (MQ=255)
                                                                                       ttCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGc                                                        >  4:26383/1‑148 (MQ=255)
                                                                                        tCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCt                                                       >  3:162791/1‑148 (MQ=255)
                                                                                           aTTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCAt                                                    <  1:179202/148‑1 (MQ=255)
                                                                                                 tGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCAt                                                                                                       >  3:192677/1‑91 (MQ=255)
                                                                                                 tGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCAt                                                                                                       <  2:357887/91‑1 (MQ=255)
                                                                                                 tGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCAt                                                                                                       >  1:357887/1‑91 (MQ=255)
                                                                                                 tGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCAt                                                                                                       <  4:192677/91‑1 (MQ=255)
                                                                                                  gCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTTGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAgcgg                                             >  3:293460/1‑148 (MQ=255)
                                                                                                  gCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCACCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGATGTACGCTCATCCAgcgg                                             >  5:156188/1‑148 (MQ=255)
                                                                                                   cTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAgcggc                                            >  8:113699/1‑148 (MQ=255)
                                                                                                    tCAATGAGCACACGCCCCTGCGCTTCCACGCCGGCTCCCCGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCCGCGCAAGGATCAAACGTGCTGTACGCTCATCCAgcggcg                                           >  1:121110/1‑148 (MQ=255)
                                                                                                     cAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAACTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAgcggcg                                           >  1:136013/1‑147 (MQ=255)
                                                                                                     cAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAACTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAgcggcg                                           >  1:142020/1‑147 (MQ=255)
                                                                                                        tGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACt                                       <  1:387066/148‑1 (MQ=255)
                                                                                                        tGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGCCGTACt                                       >  7:250034/1‑148 (MQ=255)
                                                                                                         gAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACtt                                      >  3:84368/1‑148 (MQ=255)
                                                                                                          aGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACtt                                      <  8:89049/147‑1 (MQ=255)
                                                                                                             acacGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCaaa                                                                    >  8:161575/1‑114 (MQ=255)
                                                                                                             acacGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCaaa                                                                    <  7:161575/114‑1 (MQ=255)
                                                                                                             acacGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTgcg                                  >  7:106034/1‑148 (MQ=255)
                                                                                                              cacGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTgcg                                  <  2:306182/147‑1 (MQ=255)
                                                                                                                    ccTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCaaa                           >  8:424223/1‑148 (MQ=255)
                                                                                                                     cTGCTCTTCCACGCCGGCTCCACGCAACTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAAc                          <  2:136013/148‑1 (MQ=255)
                                                                                                                     cTGCTCTTCCACGCCGGCTCCACGCAACTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAAc                          <  2:142020/148‑1 (MQ=255)
                                                                                                                      tGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGa                                                                         >  4:251817/1‑100 (MQ=255)
                                                                                                                      tGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGa                                                                         <  3:251817/100‑1 (MQ=255)
                                                                                                                       gCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCaa                                                                     <  7:240902/103‑1 (MQ=255)
                                                                                                                       gCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCaa                                                                     >  8:240902/1‑103 (MQ=255)
                                                                                                                           ttCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCg                      <  8:106034/146‑1 (MQ=255)
                                                                                                                            tCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCTCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCTCTCCAAACAGCgggt                   >  6:400785/1‑146 (MQ=255)
                                                                                                                            tCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGtt                   >  8:98030/1‑148 (MQ=255)
                                                                                                                            tCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGtt                   >  2:234823/1‑148 (MQ=255)
                                                                                                                            tCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGtt                   >  8:57997/1‑148 (MQ=255)
                                                                                                                            tCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGtt                   >  7:347537/1‑148 (MQ=255)
                                                                                                                             ccACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCgg                     <  1:376359/145‑1 (MQ=255)
                                                                                                                                cGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGTTCGTc               >  8:256026/1‑148 (MQ=255)
                                                                                                                                   cGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGt                                                            >  2:87313/1‑100 (MQ=255)
                                                                                                                                   cGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGt                                                            <  1:87313/100‑1 (MQ=255)
                                                                                                                                      cTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCaa                            <  7:258983/129‑1 (MQ=255)
                                                                                                                                      cTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCaa                            >  8:258983/1‑129 (MQ=255)
                                                                                                                                       tCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGTTCGTCCTGATgc        >  4:311178/1‑148 (MQ=255)
                                                                                                                                         cACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGt                                                            <  5:367854/94‑1 (MQ=255)
                                                                                                                                         cACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGt                                                            >  6:367854/1‑94 (MQ=255)
                                                                                                                                          aCGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCa                                                                             >  3:163117/1‑76 (MQ=255)
                                                                                                                                          aCGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCa                                                                             <  4:163117/76‑1 (MQ=255)
                                                                                                                                          aCGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGTTCGTCCTGATGcgcc     >  8:182578/1‑148 (MQ=255)
                                                                                                                                             cAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGTTCGTCCTGATGCGCcgcg  <  4:162791/148‑1 (MQ=255)
                                                                                                                                                ttGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAg                                                                                 >  7:362031/1‑66 (MQ=255)
                                                                                                                                                ttGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAg                                                                                 <  8:362031/66‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AATAGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGTTCGTCCTGATGCGCCGCG  >  NC_000913/1929174‑1929462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: