Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,552,185 A→T 5.2% intergenic (‑47/‑167) ypeA ← / → amiA GNAT family putative N‑acetyltransferase/N‑acetylmuramoyl‑l‑alanine amidase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,552,1850AT 5.2% 333.0 / 3.7 109intergenic (‑47/‑167)ypeA/amiAGNAT family putative N‑acetyltransferase/N‑acetylmuramoyl‑l‑alanine amidase I
Reads supporting (aligned to +/- strand):  ref base A (53/48);  new base T (4/2);  total (58/51)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

ATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGCGATACGATATAACATCTGGAACTTTA  >  NC_000913/2552038‑2552332
                                                                                                                                                   |                                                                                                                                                   
aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGaa                                                                                                                                                     >  3:324165/1‑148 (MQ=255)
  gTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACg                                                                                                                                                   >  3:267175/1‑148 (MQ=255)
  gTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACg                                                                                                                                                   >  5:314344/1‑148 (MQ=255)
   tcaaTTTCCGGATAGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAACGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTAACTCCATGGTATTCGTTACCTTTTTGCGGTTTAAAAGGCTGATAATGGAGTGACCgg                                                                                                                                                  >  4:390282/4‑148 (MQ=255)
    ccATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGt                                                                                                                                                 >  8:399414/1‑148 (MQ=255)
    ccATTTCCGGAGCGGTCCACGGACGCAGCAAGGCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGGATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACggg                                                                                                                                                 <  2:430655/148‑2 (MQ=255)
      aTTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTc                                                                                                                                                >  5:194003/1‑147 (MQ=255)
       tttCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGa                                                                                                                                              >  4:401177/1‑148 (MQ=255)
        ttCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGaa                                                                                                                                             >  3:281106/1‑148 (MQ=255)
        ttCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGaa                                                                                                                                             >  8:262154/1‑148 (MQ=255)
        ttCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGaa                                                                                                                                             >  7:55328/1‑148 (MQ=255)
           cGGATCGTTCCACGGAGGCAGCAAGTAGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATATCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAAtt                                                                                                                                           <  2:132880/147‑1 (MQ=255)
                   tCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAatat                                                                                                                                  >  2:85629/1‑148 (MQ=255)
                   tCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAata                                                                                                                                   >  5:122546/1‑147 (MQ=255)
                     cACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATc                                                                                                                                 <  8:55328/147‑1 (MQ=255)
                     cACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATc                                                                                                                                 <  3:401177/147‑1 (MQ=255)
                      aCGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGAATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTg                                                                                                                               <  1:252635/148‑1 (MQ=255)
                       cGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGa                                                                                                                              >  7:120970/1‑148 (MQ=255)
                         gACGCAGCAAGTCGCATCGCTCCCAAAGGGTGTTGACCTCTGCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTTCCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGACCGGTCGAATTAGCCAATATCTGACg                                                                                                                            <  1:305967/148‑1 (MQ=255)
                         gACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTCCCTTTTTCCGGGTTAAAAGGCTGATTATGGCGTGAACGGTGGAATTAGCCAATATCTGAc                                                                                                                             >  3:53350/1‑147 (MQ=255)
                         gACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACg                                                                                                                            <  5:320592/148‑1 (MQ=255)
                         gACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACg                                                                                                                            <  8:439948/148‑1 (MQ=255)
                              agcaAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAAt                                                                                                                       <  7:399414/148‑1 (MQ=255)
                                caAGTCGCAACGCTCCCAAAGGGTGATGACGTCTTCGTAATCTTCCTGGCGAAATACGCGTATGTCCATGGTATTCGTTACCTTTTTGCGGGTTAAATGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCg                                                                                                                     <  3:51313/148‑1 (MQ=255)
                                       cAACGCTCCCAAAGGGTGGTGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGATCGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGaa                                                                                                               >  5:479/1‑147 (MQ=255)
                                       cAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTGGAATTAGCCAATATCTGACGAAAATCGGTTGaa                                                                                                               >  3:55252/1‑147 (MQ=255)
                                       cAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCAGGGTATTCGTTACCTTGTTGCGGGTTACAAGGCTGATTATGGCGTGAACGGTGGAAGTAGCCAATATCTGACGAAAATCGTGTGaa                                                                                                               >  6:218033/1‑147 (MQ=255)
                                        aaCGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGaaaa                                                                                                             <  4:324165/148‑1 (MQ=255)
                                              cccAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTgg                                                                                                        >  3:384438/1‑147 (MQ=255)
                                                 aatgggTGATGACGTGTTAGAAATCTTCGTGGGGAAATCCTCGTATGTCCATTGTTTTAGTTACCTTTTTGCGGGTTTTAATGCTGATTATGGCGTGAACGGTCGAATTACCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATa                                                                                                    <  1:183377/145‑1 (MQ=255)
                                                 aaaGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCAt                                                                                                     >  5:236638/1‑147 (MQ=255)
                                                 aaaGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATa                                                                                                    <  7:158201/148‑1 (MQ=255)
                                                  aaGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATaa                                                                                                   >  7:150132/1‑148 (MQ=255)
                                                   aGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAAt                                                                                                  <  1:85629/148‑1 (MQ=255)
                                                     ggTGATGACCTCTTCGAACTCTGCCTGGCGAAATACGCGTATCTGCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGTCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATgg                                                                                                <  6:344661/148‑1 (MQ=255)
                                                      gTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGAGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATggg                                                                                               >  8:146622/1‑148 (MQ=255)
                                                         atgaCCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAg                                                                                            >  4:308837/1‑148 (MQ=255)
                                                               tctTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAata                                                                                                                                   >  7:395725/1‑103 (MQ=255)
                                                               tctTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAata                                                                                                                                   <  8:395725/103‑1 (MQ=255)
                                                                ctTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAAt                                                                                                                       <  1:270667/114‑1 (MQ=255)
                                                                ctTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAAt                                                                                                                       >  2:270667/1‑114 (MQ=255)
                                                                ctTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCaa                                                                                     >  8:406704/1‑148 (MQ=255)
                                                                ctTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCaa                                                                                     >  7:239113/1‑148 (MQ=255)
                                                                      aaTCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGTTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGaaaa                                                                                                             <  4:105220/118‑1 (MQ=255)
                                                                      aaTCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGTTTGaaaa                                                                                                             >  3:105220/1‑118 (MQ=255)
                                                                          ttCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATtgaaat                                                                           >  7:242859/1‑148 (MQ=255)
                                                                           tCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATtgaaat                                                                           >  3:348657/1‑147 (MQ=255)
                                                                            ccTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGTCATAATGGGGAGTTGTCAACTATtgaa                                                                             <  4:267175/144‑1 (MQ=255)
                                                                            ccTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATtgaaa                                                                            <  8:120970/145‑1 (MQ=255)
                                                                            ccTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATtgaa                                                                             <  6:314344/144‑1 (MQ=255)
                                                                             cTGGCGAACTACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATtgaaatga                                                                         <  4:384438/147‑1 (MQ=255)
                                                                             cTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATtgaaatga                                                                         <  2:63142/147‑1 (MQ=255)
                                                                              tGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATtgaaatgaa                                                                        >  5:228387/1‑147 (MQ=255)
                                                                              tGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATtgaaatgaa                                                                        <  6:228387/147‑1 (MQ=255)
                                                                                gCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATtgaaatgaaa                                                                       <  8:239113/146‑1 (MQ=255)
                                                                                gCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCAGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATtgaaatgaaa                                                                       <  6:194003/146‑1 (MQ=255)
                                                                                         gcgTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAAt                                                            <  3:308837/148‑1 (MQ=255)
                                                                                             ttctcCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATtct                                                         >  7:168419/2‑147 (MQ=255)
                                                                                              tctcCATGGTATTCGTTACCTATTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGCCGAAAATCGGTTGAAAAAGTGGCTTAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAACCAATtgtc                                                        >  2:385311/1‑147 (MQ=255)
                                                                                                 ccATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAAt                                                            >  6:124533/1‑140 (MQ=255)
                                                                                                 ccATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCaa                                                      >  1:179395/1‑146 (MQ=255)
                                                                                                 ccATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGGGAACGGTCGAATAAGCCAATATCTGACGAAAATCGGTTGAAAAAGAGGCATAAGGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATtctcca                                                      >  4:271875/1‑144 (MQ=255)
                                                                                                  cATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATtgaaatgaaa                                                                       <  8:112564/128‑1 (MQ=255)
                                                                                                  cATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATtgaaatgaaa                                                                       >  7:112564/1‑128 (MQ=255)
                                                                                                   aTGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATTGGGAGTTCTCAACTATTGAAATGAAAAGTAAAACAATTATCAACAg                                                   >  3:55251/1‑147 (MQ=255)
                                                                                                    tGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGc                                                  >  1:266094/1‑147 (MQ=255)
                                                                                                      gTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACTAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCaa                                                <  5:53292/147‑1 (MQ=255)
                                                                                                        aTTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAAcc                                             >  1:373519/1‑148 (MQ=255)
                                                                                                          tCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGATCGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACcgt                                           >  6:133382/1‑148 (MQ=255)
                                                                                                          tCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGATCGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACcgt                                           >  6:127518/1‑148 (MQ=255)
                                                                                                          tCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGaaaa                                                                                                             <  6:365786/82‑1 (MQ=255)
                                                                                                          tCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGaaaa                                                                                                             >  5:365786/1‑82 (MQ=255)
                                                                                                          tCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGaaaa                                                                                                             >  5:359254/1‑82 (MQ=255)
                                                                                                          tCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTa                                                                                  <  5:13293/109‑1 (MQ=255)
                                                                                                           cGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACcgt                                           <  2:266094/147‑1 (MQ=255)
                                                                                                            gTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACTGTAGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACcgtc                                          <  5:227982/147‑1 (MQ=255)
                                                                                                              tACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATtgaaatga                                                                         <  4:106577/114‑1 (MQ=255)
                                                                                                              tACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATtgaaatga                                                                         >  3:106577/1‑114 (MQ=255)
                                                                                                               aCCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTa                                       <  2:179395/147‑1 (MQ=255)
                                                                                                               aCCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTa                                       <  1:385311/147‑1 (MQ=255)
                                                                                                                ccTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAAc                                     >  1:289153/1‑148 (MQ=255)
                                                                                                                  gttttGCGGGTTAAAAGGCTGATTATGGCGTGATCGGTCGAATTAGCCAATATCTGGCGAAAGTCGGTTGAAGAAGGGGCGGAAGGGGGAGGTGTCAGCTAGTGAGATGAAAAGTAAAGCGATTCTCAGCCGCACACCGTCGGAGCgg                                   >  6:175777/2‑148 (MQ=255)
                                                                                                                    tttGCGGGTTAAAAGGCTGATTATGGCGTGATCGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACgg                                   <  5:133382/146‑1 (MQ=255)
                                                                                                                    tttGCGGGTTAAAAGGCTGATTATGGCGTGATCGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACgg                                   <  5:127518/146‑1 (MQ=255)
                                                                                                                    tttGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGAt                                 >  8:201681/1‑148 (MQ=255)
                                                                                                                      tGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGAtt                                <  7:201681/147‑1 (MQ=255)
                                                                                                                      tGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGAtt                                <  4:348657/147‑1 (MQ=255)
                                                                                                                          ggTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTAcgc                            >  6:210112/1‑147 (MQ=255)
                                                                                                                              aaaaGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGcgata                        <  6:122546/147‑1 (MQ=255)
                                                                                                                               aaaGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTAAACAGCAAACCGTCGTAACGGATTACGcgatac                       >  5:13996/1‑147 (MQ=255)
                                                                                                                                aaGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGcgatacga                     >  7:354530/1‑148 (MQ=255)
                                                                                                                                 aGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGcgatacga                     >  4:81330/1‑147 (MQ=255)
                                                                                                                                 aGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGCTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGcgatacgat                    <  5:175777/148‑1 (MQ=255)
                                                                                                                                  ggCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGcgatacgata                   <  7:406704/148‑1 (MQ=255)
                                                                                                                                   gCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAACTCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGCGATACGatat                  <  6:258822/148‑1 (MQ=255)
                                                                                                                                        ttATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACcgt                                           <  7:86291/118‑1 (MQ=255)
                                                                                                                                         tATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAAAAATTCTCAACAGCAAACCGTCGTAACGGATTACGCGATACGATATAACATc            >  3:100704/1‑148 (MQ=255)
                                                                                                                                             gCGTGAACGGTGGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGCGATACGATATAACATCTGGa        <  4:55252/148‑1 (MQ=255)
                                                                                                                                                 gAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGCGATACGATATAACATCTGGAACtt    >  3:242426/1‑148 (MQ=255)
                                                                                                                                                   aCGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGCGATACGATATAACATCTGGAACTtta  <  8:168419/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGCGATACGATATAACATCTGGAACTTTA  >  NC_000913/2552038‑2552332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: