Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,357,644 T→A 5.4% K325I (AAA→ATA)  cadA ← lysine decarboxylase, acid‑inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,357,6440TA 5.4% 397.2 / 8.3 122K325I (AAA→ATA) cadAlysine decarboxylase, acid‑inducible
Reads supporting (aligned to +/- strand):  ref base T (51/64);  new base A (4/3);  total (55/67)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

GAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGCGTGCTGGAATTCACTCTGT  >  NC_000913/4357498‑4357790
                                                                                                                                                   |                                                                                                                                                  
gagagaACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGAttt                                                                                                                                                   >  4:310378/1‑148 (MQ=255)
gagagaACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGAttt                                                                                                                                                   >  4:39087/1‑148 (MQ=255)
gagagaACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGAtt                                                                                                                                                    >  1:34758/1‑147 (MQ=255)
 agagaACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTc                                                                                                                                                  <  4:398705/148‑1 (MQ=255)
   agaACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTcac                                                                                                                                                <  8:343286/148‑1 (MQ=255)
   agaACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTca                                                                                                                                                 >  7:84105/1‑147 (MQ=255)
      ccgccgaCAGCAGTTGGGGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCCCCGCTCATACCGCCTTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACAt                                                                                                                                              <  2:91083/146‑1 (MQ=255)
      aCGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATc                                                                                                                                             >  8:250909/1‑148 (MQ=255)
           gccAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACCTCCAgtg                                                                                                                                        <  7:133022/148‑1 (MQ=255)
                  gTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCtt                                                                                                                                 <  4:88178/148‑1 (MQ=255)
                   tttGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCtt                                                                                                                                 >  1:104208/1‑147 (MQ=255)
                   tttGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtg                                                                                                                                <  3:310378/148‑1 (MQ=255)
                     tgtgAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtga                                                                                                                               <  7:250909/147‑1 (MQ=255)
                     tgtgAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtga                                                                                                                               <  2:226814/147‑1 (MQ=255)
                      gtgAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtgat                                                                                                                              >  1:228789/1‑147 (MQ=255)
                          gTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtg                                                                                                                                <  2:115705/141‑1 (MQ=255)
                          gTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtg                                                                                                                                >  1:115705/1‑141 (MQ=255)
                          gTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGt                                                                                                                         <  5:28455/148‑1 (MQ=255)
                             gACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCg                                                                                                                       <  8:386631/147‑1 (MQ=255)
                               cTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGgtg                                                                                                                    >  1:106262/1‑148 (MQ=255)
                                 gggTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGtgtt                                                                                                                  >  6:385928/1‑148 (MQ=255)
                                  tgTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGTATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGgtgt                                                                                                                   <  2:34758/145‑1 (MQ=255)
                                  ggTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGgtgt                                                                                                                   <  8:331116/146‑1 (MQ=255)
                                    tttCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTca                                                                                                                                                 >  4:286767/1‑114 (MQ=255)
                                    tttCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTca                                                                                                                                                 <  3:286767/114‑1 (MQ=255)
                                      tCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTa                                                                                                               >  1:436860/1‑146 (MQ=255)
                                      tCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTAc                                                                                                              <  6:324583/147‑1 (MQ=255)
                                       cGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTAca                                                                                                             <  2:26971/147‑1 (MQ=255)
                                        gTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTAcag                                                                                                            <  8:148273/147‑1 (MQ=255)
                                          aaaTCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTAcag                                                                                                            >  5:178144/1‑145 (MQ=255)
                                            aTCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCAAGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGAc                                                                                                       >  7:3589/1‑148 (MQ=255)
                                                cTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcat                                                                                                    >  4:320907/1‑147 (MQ=255)
                                                    cccTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAg                                                                                               >  3:44481/1‑148 (MQ=255)
                                                        tCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCgg                                                                                                                      <  3:297752/121‑1 (MQ=255)
                                                        tCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGgtgt                                                                                                                   >  8:213000/1‑124 (MQ=255)
                                                        tCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGgtgt                                                                                                                   <  7:213000/124‑1 (MQ=255)
                                                         cTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtgatg                                                                                                                             >  1:164656/1‑113 (MQ=255)
                                                         cTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtgatg                                                                                                                             <  2:164656/113‑1 (MQ=255)
                                                            cacGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGt                                                                                        <  3:39087/147‑1 (MQ=255)
                                                             acGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGgt                                                                                                                     >  7:356959/1‑117 (MQ=255)
                                                             acGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGgt                                                                                                                     <  8:356959/117‑1 (MQ=255)
                                                              cGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTgg                                                                                     <  2:228789/148‑1 (MQ=255)
                                                               ggCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTAcagc                                                                                                           >  8:87430/1‑125 (MQ=255)
                                                               ggCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTAcagc                                                                                                           <  7:93584/125‑1 (MQ=255)
                                                                gCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTa                                                                                   <  5:385928/148‑1 (MQ=255)
                                                                     cGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTa                                                                               >  7:369860/1‑147 (MQ=255)
                                                                      gCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTaga                                                                                          >  8:389604/1‑135 (MQ=255)
                                                                        tCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGCAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAg                                                                            >  5:6930/1‑147 (MQ=255)
                                                                            aCCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATg                                                                        >  7:119263/1‑147 (MQ=255)
                                                                             ccGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTa                                                                      <  6:117201/148‑1 (MQ=255)
                                                                              cGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTAc                                                                     >  4:337009/1‑148 (MQ=255)
                                                                              cGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTAc                                                                     <  2:106262/148‑1 (MQ=255)
                                                                                 aTTTACCTTCGTAAATCGGTGAGAAGTTGGTGTATGGCAACCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGGAGAGTTGGTAATTACAGCATGTACCgg                                                                  <  4:132273/148‑1 (MQ=255)
                                                                                   ttACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGc                                                                 >  7:218271/1‑147 (MQ=255)
                                                                                   ttACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGc                                                                 >  3:363402/1‑147 (MQ=255)
                                                                                     aactctacctatgatggtctgctgtacaacaccgacttcatcaagaaaacacTGGATGTGAAATCCATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAg                                                              <  4:250656‑M1/96‑1 (MQ=255)
                                                                                     aactctacctatgatggtctgctgtacaacaccgacttcatcaagaaaacacTGGATGTGAAATCCATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAg                                                              <  8:276151‑M1/96‑1 (MQ=255)
                                                                                     aactctacctatgatggtctgctgtacaacaccgacttcatcaagaaaacacTGGATGTGAAATCCATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAg                                                              <  8:269442‑M1/96‑1 (MQ=255)
                                                                                     aCCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAAtt                                                                                >  1:421149/1‑130 (MQ=255)
                                                                                     aCCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAAtt                                                                                <  2:421149/130‑1 (MQ=255)
                                                                                         tCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGt                                                                                             <  6:301214/113‑1 (MQ=255)
                                                                                         tCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTg                                                          >  2:202431/1‑148 (MQ=255)
                                                                                           gTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCg                                                        >  8:295096/1‑148 (MQ=255)
                                                                                              aaTCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGgtt                                                     >  7:437180/1‑148 (MQ=255)
                                                                                                    tGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGtt                                               <  7:295096/148‑1 (MQ=255)
                                                                                                     gtctgctgtacaacaccgacttcatcaagaaaacacTGGATGTGAAATCCATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGttt                                              >  7:276151‑M1/37‑148 (MQ=255)
                                                                                                     gagaAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcatcat                                                                                                 <  7:291418/97‑1 (MQ=255)
                                                                                                     gagaAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGttt                                                     >  8:85069/1‑141 (MQ=255)
                                                                                                     gagaAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGttt                                                     <  7:85069/141‑1 (MQ=255)
                                                                                                      agaAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGtttc                                             <  3:403786/148‑1 (MQ=255)
                                                                                                         aGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGtttcttt                                          <  3:102433/148‑1 (MQ=255)
                                                                                                           ttGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCa                                        <  8:369860/148‑1 (MQ=255)
                                                                                                            tGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcatc                                                                                                   >  3:176572/1‑88 (MQ=255)
                                                                                                            tGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcatc                                                                                                   >  3:257202/1‑88 (MQ=255)
                                                                                                            tGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcatc                                                                                                   <  4:257202/88‑1 (MQ=255)
                                                                                                            tGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCAc                                       >  6:15066/1‑148 (MQ=255)
                                                                                                              gtgtAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCAcgc                                     >  3:280213/1‑148 (MQ=255)
                                                                                                                 tAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACgcgc                                   <  4:280213/147‑1 (MQ=255)
                                                                                                                  aaGGCACCCACGCGGAGTAAAAGTGGATGGATTTCACAACCACTGTTTTCATGATGAAGTCGTTGTTGTACATCAGACCATCATAGGTATAGTTGGTAATTACAGCATATACCGGACAGGTTGCGTTAGGTCATTATTTCAcgcaat                                  >  6:401072/1‑144 (MQ=255)
                                                                                                                   aGGCTCCCACGCGGAGTCAAAGGGGATGGATTTCACATGCAGTGTTTTCTTGATGAATTCGGTGTTGTACCGCAGACCTTCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCtt                                 <  3:320907/147‑1 (MQ=255)
                                                                                                                       aCCCACGCGGAGTCAAAGAGGCTGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCaa                            <  6:93233/148‑1 (MQ=255)
                                                                                                                         ccACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATg                          <  1:202431/148‑1 (MQ=255)
                                                                                                                         ccACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATg                          >  4:332553/1‑148 (MQ=255)
                                                                                                                          cACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATgg                         <  2:436860/148‑1 (MQ=255)
                                                                                                                          cACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCAGAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCAGGCTCTTAGGAATgg                         >  2:316916/1‑148 (MQ=255)
                                                                                                                           aCGCGGAGTCAACGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGGTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGt                        <  1:110348/148‑1 (MQ=255)
                                                                                                                             gcgGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtgatga                                                                                                                            >  8:287555/1‑46 (MQ=255)
                                                                                                                             gcgGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtgatga                                                                                                                            <  7:287555/46‑1 (MQ=255)
                                                                                                                              cgGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACGATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGc                     >  1:139660/1‑148 (MQ=255)
                                                                                                                                gAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcatc                                                                                                   <  4:397423/68‑1 (MQ=255)
                                                                                                                                 aGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGCGTg                  <  8:84105/148‑1 (MQ=255)
                                                                                                                                    cAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcatc                                                                                                   <  5:158775/64‑1 (MQ=255)
                                                                                                                                    cAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcatc                                                                                                   >  6:158775/1‑64 (MQ=255)
                                                                                                                                    cAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCAAAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGCGTGCTg               >  3:406950/1‑148 (MQ=255)
                                                                                                                                      aaGTGTATGGATTTGTCATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTAGATCTTACAATCATAGGTTGAGTTGGTATTTACAGTATGTAACGTCCAGGTTTCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGAGTGCTGGa             <  2:408323/148‑1 (MQ=255)
                                                                                                                                      aaGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGCGTGCTgg              >  1:408323/1‑147 (MQ=255)
                                                                                                                                          GGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGCGTGCTGGAAtt          <  7:415833‑M1/147‑1 (MQ=255)
                                                                                                                                            ATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGCGTGCTGGAATTCAc       >  5:37048‑M1/1‑148 (MQ=255)
                                                                                                                                              GGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGCGTGCTGGAATTCActc     <  8:200749‑M1/148‑1 (MQ=255)
                                                                                                                                                  tttCACATACAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACAATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGCGTGCTGGAATTCACTCtgt  >  8:51597/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTGTTTCTTTCACGCGCTTAGCAATGGTAGCGTGCTGGAATTCACTCTGT  >  NC_000913/4357498‑4357790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: