New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4128795 =46 (1.160)5 (0.130) 5/258 4.0 10.0% coding (124/1161 nt) metB cystathionine gamma‑synthase, PLP‑dependent
?NC_000913 4128835 = 46 (1.220)coding (164/1161 nt) metB cystathionine gamma‑synthase, PLP‑dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGA  >  NC_000913/4128649‑4128924
                                                                                                                                                  |                                                                                                                                 
cAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGTCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCAAAGATCCATCTTTCAAGCACCTATAACTTTACCGGATTTAATGAACcgc                                                                                                                                   >  6:163951/1‑147 (MQ=255)
      ctaGGGAACTTCTTCACATGACGAGTAAACAGGCCTCCTTCGCAGTGCGTAGCGGGTTAATTGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCAt                                                                                                                           <  8:64949/147‑1 (MQ=255)
         gggAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGa                                                                                                                         <  5:19693/148‑1 (MQ=255)
         gggAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGa                                                                                                                         >  6:19693/1‑148 (MQ=255)
                  atcaCATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACtcgcgt                                                                                                               >  2:64572/1‑149 (MQ=255)
                     acaTGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAAACGCGCGCGCATGATTACtcgcgtcgc                                                                                                            >  4:119022/1‑149 (MQ=255)
                       aTGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCgg                                                                                                          >  6:30173/1‑149 (MQ=255)
                                          ccaTCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGAtgtg                                                                                       <  4:150217/149‑1 (MQ=255)
                                           caTCGCATTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGGCGCGGCAACCCAACGCGCGATGTgg                                                                                      <  5:163951/149‑1 (MQ=255)
                                                     cGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGCTCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTgc                                                                            >  7:152389/1‑149 (MQ=255)
                                                      gTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTgcg                                                                           >  6:1108/1‑149 (MQ=255)
                                                       tAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGc                                                                                >  1:57465/1‑143 (MQ=255)
                                                       tAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGc                                                                                <  2:57465/143‑1 (MQ=255)
                                                                  aTGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTg                                                               <  5:1108/149‑1 (MQ=255)
                                                                            aCAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGgtggtg                                                     >  5:120060/1‑149 (MQ=255)
                                                                               gTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGtggtgctg                                                  <  6:120060/149‑1 (MQ=255)
                                                                                  tGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGtggtgctggtg                                               <  1:64572/149‑1 (MQ=255)
                                                                                     ttGCGTTGTCCCACCGATCCATCTTTCCAGCAACTATAACTATACCGGAATTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAg                                            >  2:52172/1‑149 (MQ=255)
                                                                                         gTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGtact                                        >  7:93009/1‑149 (MQ=255)
                                                                                          ttGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGc                                                                                <  1:18006/108‑1 (MQ=255)
                                                                                          ttGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGc                                                                                >  2:18006/1‑108 (MQ=255)
                                                                                            gTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGtactta                                      <  8:152389/148‑1 (MQ=255)
                                                                                            gTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGtactta                                      <  5:30173/148‑1 (MQ=255)
                                                                                             tCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCACCGCGCGATGTGGTTCa                                                                                  >  2:101853/1‑103 (MQ=255)
                                                                                             tCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCa                                                                                  <  1:101853/103‑1 (MQ=255)
                                                                                               ccACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGgtggtg                                                     <  7:61809/130‑1 (MQ=255)
                                                                                               ccACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGgtggtg                                                     >  8:61809/1‑130 (MQ=255)
                                                                                                 aCCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATa                                >  7:155229/1‑149 (MQ=255)
                                                                                                      tCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCgg                            >  5:46148/1‑148 (MQ=255)
                                                                                                      tCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGc                           <  6:46148/149‑1 (MQ=255)
                                                                                                         aTCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGtggtgc                                                    <  4:134244/121‑1 (MQ=255)
                                                                                                         aTCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGtggtgc                                                    >  3:134244/1‑121 (MQ=255)
                                                                                                         aTCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGtactta                                      >  4:151313/1‑135 (MQ=255)
                                                                                                         aTCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGtactta                                      <  3:151313/135‑1 (MQ=255)
                                                                                                         aTCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATg                        >  5:177392/1‑149 (MQ=255)
                                                                                                                cAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGa                 <  8:155229/149‑1 (MQ=255)
                                                                                                                cAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGa                 >  1:80947/1‑149 (MQ=255)
                                                                                                                      cTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGtacttact                                    >  6:12389/1‑124 (MQ=255)
                                                                                                                      cTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGtacttact                                    <  5:12389/124‑1 (MQ=255)
                                                                                                                       tataACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCt          >  5:123412/1‑149 (MQ=255)
                                                                                                                        ataACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAggtg                                                        >  2:90927/1‑102 (MQ=255)
                                                                                                                        ataACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAggtg                                                        <  1:90927/102‑1 (MQ=255)
                                                                                                                          aaCTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGGTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTc                      <  8:69210/134‑1 (MQ=255)
                                                                                                                          aaCTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTc                      >  7:69210/1‑134 (MQ=255)
                                                                                                                             tttACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAAc    >  6:98855/1‑149 (MQ=255)
                                                                                                                                aCCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGa  <  6:123412/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                 
CAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGA  >  NC_000913/4128649‑4128924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.