Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 897,973 Δ1 bp 5.2% intergenic (+44/‑16) potI → / → ybjO putrescine ABC transporter permease/DUF2593 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913897,9730T. 5.2% 302.3 / 9.3 96intergenic (+44/‑16)potI/ybjOputrescine ABC transporter permease/DUF2593 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (51/40);  new base . (2/3);  total (53/43)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01

ATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGATT  >  NC_000913/897828‑898117
                                                                                                                                                  |                                                                                                                                                
aTCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTcgc                                                                                                                                               >  1:233641/1‑149 (MQ=255)
 tCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTcgcg                                                                                                                                              <  5:226089/149‑1 (MQ=255)
      ggCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGACATGCGCGGCAtt                                                                                                                                         <  2:9385/149‑1 (MQ=255)
       gcgcGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTg                                                                                                                                        <  7:146095/149‑1 (MQ=255)
        cgcgGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGACATGCGCGGCAttat                                                                                                                                       <  5:59172/149‑3 (MQ=255)
         gcgGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGtt                                                                                                                                      <  3:118427/149‑1 (MQ=255)
         gcgGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGtt                                                                                                                                      <  6:286854/149‑1 (MQ=255)
            gTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACTCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGtttt                                                                                                                                    >  3:92635/1‑148 (MQ=255)
             tCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCa                                                                                                                                  <  5:130256/149‑1 (MQ=255)
               ggAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATg                                                                                                                                <  1:115158/149‑1 (MQ=255)
                      tCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCa                                                                                                                                  <  5:231143/140‑1 (MQ=255)
                      tCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCa                                                                                                                                  >  6:231143/1‑140 (MQ=255)
                      tCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGAc                                                                                                                          >  7:277214/1‑148 (MQ=255)
                              aTCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGtt                                                                                                                  <  4:92635/148‑1 (MQ=255)
                              aTCGACTGGTATCTGATGTATCTCGAAGAAAAACAGCTTATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGATACATAATGACGCTCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGtt                                                                                                                  <  1:62624/148‑1 (MQ=255)
                               tCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTgg                                                                                                                <  8:277214/149‑1 (MQ=255)
                               tCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTg                                                                                                                 <  4:12671/148‑1 (MQ=255)
                                  ccTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGAt                                                                                                             >  4:271377/1‑149 (MQ=255)
                                    tGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGAttt                                                                                                           >  2:254878/1‑149 (MQ=255)
                                       tATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGAttttt                                                                                                         >  4:288699/1‑148 (MQ=255)
                                       tATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGAttttt                                                                                                         >  6:69311/1‑148 (MQ=255)
                                           tGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCAGGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAg                                                                                                     >  7:289902/1‑148 (MQ=255)
                                             aTGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGa                                                                                                    <  3:271377/147‑1 (MQ=255)
                                                gCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACa                                                                                               <  5:210885/149‑1 (MQ=255)
                                                    gcgcAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATc                                                                                             <  5:69311/147‑1 (MQ=255)
                                                     cgcAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCtt                                                                                           >  8:190266/1‑148 (MQ=255)
                                                        aGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCAtc                                                                                       >  7:49693/1‑149 (MQ=255)
                                                         gAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCAtct                                                                                      >  7:271600/1‑149 (MQ=255)
                                                          aaaaaCAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCAtct                                                                                      >  2:272507/1‑148 (MQ=255)
                                                           aaaaCAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCa                                                                                    <  3:220484/149‑1 (MQ=255)
                                                            aaaCAGCGGATTCGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTACATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCAt                                                                                   <  6:240387/149‑1 (MQ=255)
                                                              aCAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGc                                                                                 >  5:218901/1‑149 (MQ=255)
                                                              aCAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTGGGGATTTTTTAAGATAACATCTTCATCTCATGc                                                                                 >  2:166831/1‑149 (MQ=255)
                                                                  cGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGc                                                                             <  8:271600/149‑1 (MQ=255)
                                                                    gATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTaa                                                                                                      <  5:96114/122‑1 (MQ=255)
                                                                    gATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTaa                                                                                                      >  6:96114/1‑122 (MQ=255)
                                                                         gcgACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAAtg                                                                      >  2:229701/1‑149 (MQ=255)
                                                                          cgACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAAtgt                                                                     <  1:329224/149‑1 (MQ=255)
                                                                          cgACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAAtgt                                                                     <  2:36679/149‑1 (MQ=255)
                                                                            aCATCCAGCGTGCAAGACGTGGCTGAAGACACTAATATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGc                                                                   <  7:306268/149‑1 (MQ=255)
                                                                             cATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGcc                                                                  <  3:71285/149‑1 (MQ=255)
                                                                              aTCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCt                                                                 >  8:244765/1‑149 (MQ=255)
                                                                               tCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTg                                                                >  2:179345/1‑149 (MQ=255)
                                                                                  aGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTgcgc                                                             >  1:269431/1‑149 (MQ=255)
                                                                                     gTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTgg                                                          <  4:316684/149‑1 (MQ=255)
                                                                                      tGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCTCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGt                                                         >  3:135625/1‑149 (MQ=255)
                                                                                       gCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGt                                                         >  3:149245/1‑148 (MQ=255)
                                                                                       gCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGt                                                         <  4:149245/148‑1 (MQ=255)
                                                                                       gCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTg                                                        >  1:20327/1‑149 (MQ=255)
                                                                                       gCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTg                                                        >  4:224473/1‑149 (MQ=255)
                                                                                         aaGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGCATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTg                                                        >  5:265069/1‑147 (MQ=255)
                                                                                              gTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTgg                                                 <  8:49693/149‑1 (MQ=255)
                                                                                                ggCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGTAGGTggcg                                               >  4:312359/1‑149 (MQ=255)
                                                                                                 gCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTggcgg                                              >  2:172566/1‑149 (MQ=255)
                                                                                                   tGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACTTTGGGATTTTTTAAGAAAACATCTTCAACTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGgcggcg                                            >  2:64101/1‑149 (MQ=255)
                                                                                                   tGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGgcggcg                                            >  4:81993/1‑149 (MQ=255)
                                                                                                            tAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCAtctc                                                                                     >  1:177893/1‑99 (MQ=255)
                                                                                                            tAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCAtctc                                                                                     <  2:177893/99‑1 (MQ=255)
                                                                                                            tAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGGGGCGCTCGCCat                                    >  5:42252/1‑148 (MQ=255)
                                                                                                            tAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCat                                    >  2:177118/1‑148 (MQ=255)
                                                                                                            tAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCat                                    >  3:191131/1‑148 (MQ=255)
                                                                                                            tAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCat                                    >  1:191279/1‑148 (MQ=255)
                                                                                                            tAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCat                                    >  5:333531/1‑148 (MQ=255)
                                                                                                              aaaTTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGACGACGAAACGCTGGGATTTTTTAAGAACACATCTTCATCTCCTGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCatta                                  <  1:166831/148‑1 (MQ=255)
                                                                                                              aaaTTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCatta                                  <  2:233641/148‑1 (MQ=255)
                                                                                                                      cAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAACCGTTGGGAGTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATcc                         >  3:246904/1‑149 (MQ=255)
                                                                                                                       aaCCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAACCATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCg                        >  2:21112/1‑149 (MQ=255)
                                                                                                                           tGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCatta                                  >  2:198175/1‑135 (MQ=255)
                                                                                                                           tGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCatta                                  <  1:198175/135‑1 (MQ=255)
                                                                                                                             tgaaaacaaTGCCGCGACATGCGCGGCATTATTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCt                  >  1:113978/8‑149 (MQ=255)
                                                                                                                             gCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGc                    <  1:254878/147‑1 (MQ=255)
                                                                                                                               tACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTc                 >  6:103785/1‑148 (MQ=255)
                                                                                                                               tACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCg                >  3:220167/1‑149 (MQ=255)
                                                                                                                               tACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCg                >  6:61985/1‑149 (MQ=255)
                                                                                                                                aCATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCa                                                                                    <  5:46809/80‑1 (MQ=255)
                                                                                                                                aCATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCa                                                                                    <  5:202874/80‑1 (MQ=255)
                                                                                                                                aCATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCa                                                                                    >  6:46809/1‑80 (MQ=255)
                                                                                                                                aCATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCa                                                                                    >  6:202874/1‑80 (MQ=255)
                                                                                                                                 cATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGcc                                                                  >  4:226871/1‑97 (MQ=255)
                                                                                                                                 cATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGcc                                                                  <  3:226871/97‑1 (MQ=255)
                                                                                                                                   tAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGt            <  4:191131/149‑1 (MQ=255)
                                                                                                                                    aaTGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCt                                          <  1:262996/118‑1 (MQ=255)
                                                                                                                                    aaTGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCt                                          >  2:262996/1‑118 (MQ=255)
                                                                                                                                    aaTGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTCGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTg           >  6:226174/1‑149 (MQ=255)
                                                                                                                                     aTGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTCGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGc          <  5:226174/149‑1 (MQ=255)
                                                                                                                                      tGCCGCGACATGCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCa                                     <  6:73641/121‑1 (MQ=255)
                                                                                                                                      tGCCGCGACATGCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATATCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCa                                     >  5:73641/1‑121 (MQ=255)
                                                                                                                                      tGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCt         <  7:190266/149‑1 (MQ=255)
                                                                                                                                        ccGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCtga       <  6:218901/149‑1 (MQ=255)
                                                                                                                                        ccGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCATCATCTCATGCTCGCCTGAATGTGACTTCGATGGTGCAGATCGCGGAGCTCGCAATTATTATGATACGTGGACTCGCCGTg           >  5:284405/1‑145 (MQ=255)
                                                                                                                                         cgcgCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCtgat      >  7:102586/1‑149 (MQ=255)
                                                                                                                                          gcgcATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCtgat      >  3:88454/1‑148 (MQ=255)
                                                                                                                                          gcgcATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCtgat      >  8:296308/1‑148 (MQ=255)
                                                                                                                                           cgcATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCtgatga    <  3:224473/149‑1 (MQ=255)
                                                                                                                                           cgcATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCtgatga    >  3:297556/1‑149 (MQ=255)
                                                                                                                                              cATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGAtt  <  6:333531/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGATT  >  NC_000913/897828‑898117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: