Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,395,592 C→G 5.2% D223H (GAC→CAC)  yhdP ← DUF3971‑AsmA2 domains protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,395,5920CG 5.2% 322.0 / 3.7 94D223H (GAC→CAC) yhdPDUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand):  ref base C (39/50);  new base G (2/3);  total (41/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

CCCAACCAGCTGGCACCGCCCTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCTGGAGAGGCTTACCAGGCCTTCCGCACGGTGTCGACGTGGATCG  >  NC_000913/3395444‑3395737
                                                                                                                                                    |                                                                                                                                                 
cccAACCAGCTGGCACCGCCCTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTc                                                                                                                                                   >  8:126995/1‑149 (MQ=255)
cccAACCAGCTGGCACCGCCCTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTc                                                                                                                                                   >  2:134407/1‑149 (MQ=255)
  cAACCAGCTGGCACCGCCCTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGa                                                                                                                                                 <  3:180582/149‑1 (MQ=255)
     ccAGCTGGCACCGCCCTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGt                                                                                                                                              <  5:177832/149‑1 (MQ=255)
       aGCTGGCACCGCCCTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCa                                                                                                                                            <  7:251963/149‑1 (MQ=255)
            gCACCGCCCTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCa                                                                                                                                            >  6:100071/1‑144 (MQ=255)
            gCACCGCCCTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCa                                                                                                                                            <  5:100071/144‑1 (MQ=255)
             cACCGCCCTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGcc                                                                                                                                      <  6:290049/149‑1 (MQ=255)
              aCCGCCCTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATg                                                                                                                                               >  1:13286/1‑139 (MQ=255)
              aCCGCCCTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATg                                                                                                                                               <  2:13286/139‑1 (MQ=255)
              aCCGCCCTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCt                                                                                                                                     <  8:5856/149‑1 (MQ=255)
              aCCGCCCTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCt                                                                                                                                     >  3:146580/1‑149 (MQ=255)
               ccGCCCTGTTTCAGCCAGACGGCACCGCCGGTGACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTGTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCCCGGCTTCAGGTCGATGTCATCCGCCTg                                                                                                                                    <  6:217182/149‑1 (MQ=255)
                cGCCCTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTCCAGGCGGATGACATCGACCTg                                                                                                                                    >  6:65209/1‑148 (MQ=255)
                  cccTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAg                                                                                                                                 >  7:253328/1‑149 (MQ=255)
                  cccTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCAGCCTGGAg                                                                                                                                 >  2:317821/1‑149 (MQ=255)
                   ccTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGc                                                                                                                                <  7:126995/149‑1 (MQ=255)
                   ccTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGc                                                                                                                                <  2:163954/149‑1 (MQ=255)
                   ccTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGc                                                                                                                                <  1:134407/149‑1 (MQ=255)
                    cTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGTATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGcc                                                                                                                               <  2:271988/149‑1 (MQ=255)
                    cTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTCCAGGCGGATGACATCGACCTGAAGcc                                                                                                                               <  5:65209/149‑1 (MQ=255)
                    cTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCATTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGAAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCCTCCGCCTGGAGcc                                                                                                                               <  3:62485/149‑1 (MQ=255)
                       tttCAGCCAGACGTCACCGCGGGTTACATGGCCTTTGTCGTTCGTCATCCAGCCTTCAAGGGAGAACTGTTCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAg                                                                                                                             <  8:286927/148‑1 (MQ=255)
                       tttCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGa                                                                                                                            <  4:120072/149‑1 (MQ=255)
                         tCAGCGAGACGTCATCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTGAAGCGAGAACTGAGCCGTTTGCAGCGCAATATTGTCCTTCATCCATTTACCGAGCCTCGGCTTCAGGTGGATGTCATCCGCCTGGAGCCAGAc                                                                                                                           <  1:214850/148‑1 (MQ=255)
                            gCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCCTCCATTTACCGAGCCACGGCTTCAGGTCGACGTCATCCGCCTGGAGCCAGACGCGa                                                                                                                       <  7:133671/149‑1 (MQ=255)
                            gCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGa                                                                                                                       <  5:50750/149‑1 (MQ=255)
                             ccAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTgg                                                                                                                                   <  6:233954/136‑1 (MQ=255)
                             ccAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTgg                                                                                                                                   >  5:233954/1‑136 (MQ=255)
                             ccAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGAc                                                                                                                      >  8:309004/1‑149 (MQ=255)
                                gACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCCCGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCAt                                                                                                                   >  2:317637/1‑149 (MQ=255)
                                gACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATg                                                                                                                                               <  3:74720/121‑1 (MQ=255)
                                gACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATg                                                                                                                                               >  4:74720/1‑121 (MQ=255)
                                gACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCAt                                                                                                                   <  8:253328/149‑1 (MQ=255)
                                   gTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGc                                                                                                                >  2:25584/1‑149 (MQ=255)
                                    tCACCGCCGGTTACATCGCCTGTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCTAGCCACGGCTTCAGGTCGATGTACTCCGCCTGGAGACATACGCGACCAGTGCt                                                                                                               >  4:106065/1‑149 (MQ=255)
                                     cACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCCCGGCTTCAGGTCGATGTCATCCGCCGGGAGCCAGACGCGACCATTGCt                                                                                                               >  7:334198/1‑148 (MQ=255)
                                        cgccgGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTaac                                                                                                           <  7:296250/149‑1 (MQ=255)
                                          ccgGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTaacaa                                                                                                         <  1:317821/149‑1 (MQ=255)
                                                  aTCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCtcat                                                                                                 >  7:190064/1‑149 (MQ=255)
                                                      ccTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCatcatc                                                                                             >  6:333282/1‑149 (MQ=255)
                                                       cTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCATGAATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATAATcg                                                                                            >  3:222820/1‑149 (MQ=255)
                                                        tttGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCAGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATcgc                                                                                           >  3:214327/1‑149 (MQ=255)
                                                         ttGTCTATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGa                                                                                                                       <  8:310808/120‑1 (MQ=255)
                                                         ttGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGa                                                                                                                       >  7:310808/1‑120 (MQ=255)
                                                          tGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGCCCATTGCTTAACAACCCCTCATCATcgcg                                                                                          >  7:14134/1‑148 (MQ=255)
                                                            tCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGTGCaa                                                                                       <  7:309004/149‑1 (MQ=255)
                                                              gATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAAt                                                                                     >  3:145542/1‑149 (MQ=255)
                                                                tCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATc                                                                                    >  8:236899/1‑148 (MQ=255)
                                                                  gTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCAt                                                                                 >  1:211512/1‑149 (MQ=255)
                                                                  gTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGGCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCAt                                                                                 <  1:307694/149‑1 (MQ=255)
                                                                   tCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATg                                                                                <  3:147308/149‑1 (MQ=255)
                                                                        cAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCAc                                                                           >  5:107442/1‑149 (MQ=255)
                                                                         aGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGAc                                                                                                                           >  5:178070/1‑100 (MQ=255)
                                                                         aGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGAc                                                                                                                           <  6:178070/100‑1 (MQ=255)
                                                                         aGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCAcc                                                                          >  3:265375/1‑149 (MQ=255)
                                                                         aGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTACATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCAcc                                                                          >  2:222919/1‑149 (MQ=255)
                                                                          gCCTTCACGGGAGAACTCTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCt                                                                         <  3:106065/149‑1 (MQ=255)
                                                                           ccTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGCCCACTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTg                                                                        >  4:63311/1‑149 (MQ=255)
                                                                            cTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGc                                                                       <  4:145542/149‑1 (MQ=255)
                                                                              tCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATcg                                                                                            <  2:118776/126‑1 (MQ=255)
                                                                              tCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATcg                                                                                            >  1:118776/1‑126 (MQ=255)
                                                                                       aaCTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTg                                                            <  7:310386/149‑1 (MQ=255)
                                                                                         cTGTGCCGTTTCCAGCGCAATAGTGTCCTGCATCCAGTTACCGCGACACGGCTTCATGGCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCt                                                          <  3:35445/149‑1 (MQ=255)
                                                                                               cGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGAGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCgg                                                    >  7:265249/1‑149 (MQ=255)
                                                                                                gTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGt                                                   <  1:4189/149‑1 (MQ=255)
                                                                                                     cAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGcc                                                               <  8:326006/132‑1 (MQ=255)
                                                                                                     cAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGcc                                                               >  7:326006/1‑132 (MQ=255)
                                                                                                      aGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCt                                             >  4:269747/1‑149 (MQ=255)
                                                                                                       gcgcAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCAcc                                                                          >  8:25546/1‑119 (MQ=255)
                                                                                                       gcgcAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCAcc                                                                          <  7:25546/119‑1 (MQ=255)
                                                                                                       gcgcAATATTGTCATGCAGCCATTTACCGAGCCACGTCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCTg                                            <  8:334198/149‑1 (MQ=255)
                                                                                                           aaTATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCTGgaga                                        <  2:16562/149‑1 (MQ=255)
                                                                                                           aaTATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCTGga                                          >  2:182621/1‑147 (MQ=255)
                                                                                                           aaTATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTCTCCGGTAAGGCTGga                                          >  7:277550/1‑147 (MQ=255)
                                                                                                           aaTATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCGGCATTACGCCGTGCTGTCCGGTAAGGCTGga                                          >  2:263513/1‑147 (MQ=255)
                                                                                                                  tCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCTGGAGAGGCTTAc                                 <  8:283082/149‑1 (MQ=255)
                                                                                                                    cTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCTGGAGAGGCTTACCa                               <  4:265375/149‑1 (MQ=255)
                                                                                                                      gCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCTGGAGAGGCTTACCAgg                             >  6:132189/1‑149 (MQ=255)
                                                                                                                       cATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCAc                                                                           <  1:166616/102‑1 (MQ=255)
                                                                                                                       cATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCAc                                                                           >  2:166616/1‑102 (MQ=255)
                                                                                                                        aTCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCTGGAGAGGCTTACCAGGcc                           >  4:233316/1‑149 (MQ=255)
                                                                                                                             tttACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGc                                                                <  6:311496/107‑1 (MQ=255)
                                                                                                                             tttACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGc                                                                >  5:311496/1‑107 (MQ=255)
                                                                                                                                aCCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCAc                                                                           <  8:148661/93‑1 (MQ=255)
                                                                                                                                aCCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCAc                                                                           >  7:148661/1‑93 (MQ=255)
                                                                                                                                 ccGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCTGGAGAGGCTTACCAGGCCTTCCGCACg                  <  8:265249/149‑1 (MQ=255)
                                                                                                                                      gtctggCTCCAGGCGGATGACATCGACCTGAAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCTGGAGAGGCTTACCAGGCCTTCCGCACGGTGTc             >  4:185269/5‑149 (MQ=38)
                                                                                                                                      ccACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCTGGAGAGGCTTACCAGGCCTTCCGCACGGTGTc             <  8:190064/149‑1 (MQ=255)
                                                                                                                                       cACGGCTTCAGGTCGATTTCATCCCCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATAACGCCGTGCTGTCCGGTAAGGCTGGAGAGGCTTACCAGGCCTTCCGCACGGTGTCg            <  2:150254/149‑1 (MQ=255)
                                                                                                                                           gCTTCAGGTGGTTGTCATCCGCCTGGAGCCAGACGCGACGATTGCTTAACAACCCCTCTTCATCGCGCAAATCGATGCGCACCTGCATCACGCCGTGCTGTCCGGTTAGGCTGGAGAGGCTTACCAGGCCTTCCGCACGGTGTCGACGt        <  4:222820/149‑1 (MQ=255)
                                                                                                                                           gCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCTGGAGAGGCTTACCAGGCCTTCCGCACGGTGTCGACGt        <  1:222919/149‑1 (MQ=255)
                                                                                                                                                aGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCCTGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCTGGAGAGGCTTACCAGGCCTTCCGCACGGTGTCGACGTGGAt    <  4:214327/148‑1 (MQ=255)
                                                                                                                                                aGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCTGGAGAGGCTTACCAGGCCTTCCGCACGGTGTCGACGTGGATc   <  2:141908/149‑1 (MQ=255)
                                                                                                                                                aGGTCGATGGCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCTGGAGAGGCTTACCAGGCCTTCCGCACGGTGTCGACGTGGATc   <  6:107442/149‑1 (MQ=255)
                                                                                                                                                 ggTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCTGGAGAGGCTTACCAGGCCTTCCGCACGGTGTCGACGTGGATCg  <  2:211512/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CCCAACCAGCTGGCACCGCCCTGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCATTTACCGAGCCACGGCTTCAGGTCGATGTCATCCGCCTGGAGCCAGACGCGACCATTGCTTAACAACCCCTCATCATCGCGCAAATCCATGCGCACCTGCATCACGCCGTGCTGTCCGGTAAGGCTGGAGAGGCTTACCAGGCCTTCCGCACGGTGTCGACGTGGATCG  >  NC_000913/3395444‑3395737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: