Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,792,767 A→T 5.6% intergenic (‑216/+59) kbl ← / ← yibB glycine C‑acetyltransferase/YibB family protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,792,7670AT 5.6% 335.0 / 8.2 107intergenic (‑216/+59)kbl/yibBglycine C‑acetyltransferase/YibB family protein, function unknown
Reads supporting (aligned to +/- strand):  ref base A (50/51);  new base T (3/3);  total (53/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.19e-01

Only 100 of 111 total aligned reads displayed.

CGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTC  >  NC_000913/3792620‑3792914
                                                                                                                                                    |                                                                                                                                                   
cgTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCaaa                                                                                                                                                    <  1:124238/149‑1 (MQ=255)
cgTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCaaa                                                                                                                                                    <  8:27058/149‑1 (MQ=255)
    aaCACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGca                                                                                                                                                 <  3:118360/148‑1 (MQ=255)
     acacTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGcat                                                                                                                                                >  8:316789/1‑148 (MQ=255)
         ttCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAttt                                                                                                                                           >  1:134356/1‑149 (MQ=255)
           cTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCAtttt                                                                                                                                          <  3:29763/148‑1 (MQ=255)
            tGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTc                                                                                                                                        >  6:53728/1‑149 (MQ=255)
              cAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAttttttc                                                                                                                                       <  4:25790/148‑3 (MQ=255)
              cAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCATTTTTTCt                                                                                                                                      <  2:134356/149‑1 (MQ=255)
              cAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTca                                                                                                                                       >  4:246691/1‑147 (MQ=255)
              cAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCt                                                                                                                                      <  4:205900/149‑1 (MQ=255)
              cAAAATTCAGCATAAATAACGTCTAACTAGATCGCTCTAAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATCATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTc                                                                                                                                        <  2:147392/147‑1 (MQ=255)
               aaaaTTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTa                                                                                                                                     >  3:249392/1‑149 (MQ=255)
                 aaTTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGCTTTGGCTGCGCCTAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTAtc                                                                                                                                   <  7:238679/149‑1 (MQ=255)
                  aTTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTAtct                                                                                                                                  >  7:189269/1‑149 (MQ=255)
                        gATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTg                                                                                                                            >  7:34292/1‑149 (MQ=255)
                          taaataaCGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGt                                                                                                                          <  1:195431/149‑1 (MQ=255)
                            aataaCGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTa                                                                                                                         <  7:287005/148‑1 (MQ=255)
                              taaCGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATc                                                                                                                       >  7:290784/1‑148 (MQ=255)
                                 agtgtAACTTGATGTCTCTCAACAACTACAGATAGTAGAAAAACTGTACAGATTTGGCTGCGCATCTAGTTGATTGATTTTACTGCATTGATATAAAAAGAAATATTGATCCCAAAGCATCATTTTTCCTATCTCAGTTTGAATCaaat                                                                                                                   <  2:147333/148‑2 (MQ=255)
                                  gtgtAACTTGATGTCTCTCAACACCTACATTTATTATAAAAACTGTACAGTTTTTGCTGGGCATAATGATTATTGATTTTTCTGCATTGATATAAAAATAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAAc                                                                                                                  <  5:187311/149‑1 (MQ=255)
                                  gtgtAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAAc                                                                                                                  <  5:333862/149‑1 (MQ=255)
                                  gtgtAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAAc                                                                                                                  <  8:58701/149‑1 (MQ=255)
                                    gtAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGc                                                                                                                >  2:328748/1‑149 (MQ=255)
                                       acaagATGTCTCTCAACACCTACAGATACTAGAAAAACTGAACACTTTTGGCTCGGCATAAAGATGATAGATTTTACTGCATTGATATAAAAAGAAAAAAAGAAGCCAAAGCATGATTTATCCTATCTCAGTTGGTATCAAAACGCAAc                                                                                                             >  4:209257/5‑149 (MQ=255)
                                       aCTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTa                                                                                                              <  4:301319/148‑1 (MQ=255)
                                       aCTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTa                                                                                                              <  5:236459/148‑1 (MQ=255)
                                       aCTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTa                                                                                                              <  2:64745/148‑1 (MQ=255)
                                         ttGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTAcc                                                                                                            >  8:208683/1‑148 (MQ=255)
                                            aTGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCAc                                                                                                         <  7:187324/148‑1 (MQ=255)
                                            aTGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATCATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCATATCTCAGTTGGTATCAAAACGCTACCCAc                                                                                                         >  6:305867/1‑148 (MQ=255)
                                                ctctcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAAt                                                                                                     <  8:189269/148‑1 (MQ=255)
                                                 tctcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGATGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAATAAAAAATGATGCCAACGCATCATTTTTCCTATCTCAGTTGTGATCAAAACGCTACCCACTAATg                                                                                                    >  5:208825/1‑148 (MQ=255)
                                                   tcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAACAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATg                                                                                                    <  1:223378/146‑1 (MQ=255)
                                                   tcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAttttttc                                                                                                                                       >  1:160192/1‑109 (MQ=39)
                                                   tcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAttttttc                                                                                                                                       <  2:160192/111‑3 (MQ=39)
                                                   tcAACAACTACATATATTATAAAAACTTTACAGTTTTGGATGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATg                                                                                                    <  1:160696/146‑1 (MQ=255)
                                                     aaCACCTACAGATAGTATAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTa                                                                                                              <  5:173226/134‑1 (MQ=255)
                                                     aaCACCTACAGATAGTAGAATAACTGTACAGTTTTGGCTGGGAATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAg                                                                                                 <  6:64594/147‑1 (MQ=255)
                                                     aaCACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTa                                                                                                              >  6:173226/1‑134 (MQ=255)
                                                     aaCACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAg                                                                                                 <  5:309194/147‑1 (MQ=255)
                                                          cTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAAc                                                                                                                  >  7:123984/1‑125 (MQ=255)
                                                              aGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTAtt                                                                                      >  3:136201/1‑149 (MQ=255)
                                                                aTAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTc                                                                                                                                        >  2:273473/1‑97 (MQ=255)
                                                                aTAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAAc                                                                                           <  2:145551/142‑1 (MQ=255)
                                                                aTAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAAc                                                                                           >  1:145551/1‑142 (MQ=255)
                                                                aTAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTAtttt                                                                                    <  7:208683/149‑1 (MQ=255)
                                                                 tagtagAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTc                                                                                   <  8:34292/149‑1 (MQ=255)
                                                                   gtagAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCtt                                                                                 <  7:316789/149‑1 (MQ=255)
                                                                   gtagAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCtt                                                                                 <  8:290784/149‑1 (MQ=255)
                                                                       aaaaaCTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGa                                                                               >  4:236807/1‑147 (MQ=255)
                                                                            cTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGa                                                                          >  2:79451/1‑148 (MQ=255)
                                                                            cTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGaa                                                                         >  6:69214/1‑148 (MQ=255)
                                                                                aCAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGTAAACCTATTTTCTTGAAAGGAAAATtc                                                                    >  7:288461/1‑149 (MQ=255)
                                                                                aCAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATtc                                                                    >  7:45102/1‑149 (MQ=255)
                                                                                  aGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATtctc                                                                  <  1:79799/149‑1 (MQ=255)
                                                                                   gTTTTGGCTGGTCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAACTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCa                                                                 <  7:340448/149‑1 (MQ=255)
                                                                                         gCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCt                                                           <  5:78049/149‑1 (MQ=255)
                                                                                         gCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCt                                                           >  2:159536/1‑149 (MQ=255)
                                                                                            gggCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCa                                                        >  2:75230/1‑149 (MQ=255)
                                                                                                 tacggatgatTGATGTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAATCATCATTTTTCCTATCTCAGTTGGTAGCAAAACGCTACACACTAATGGAGGGAAACCTATTTTCTTGCAAGGAAAATTCTCAGCGCCTGCAttttt                                                   <  6:208825/145‑1 (MQ=255)
                                                                                                 tAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAttttt                                                   <  4:179613/149‑1 (MQ=255)
                                                                                                   atgatgATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGc                                                 <  5:69214/149‑1 (MQ=255)
                                                                                                      atgatTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGccc                                               <  5:12633/148‑1 (MQ=255)
                                                                                                          ttgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaaa                                          <  5:53728/149‑1 (MQ=255)
                                                                                                          ttgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaaa                                          >  3:187708/1‑149 (MQ=255)
                                                                                                             attTTACTGCATTGATATAAAAAGAAAAACTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGAATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtg                                       <  3:216844/149‑1 (MQ=255)
                                                                                                             attTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtg                                       >  7:331320/1‑149 (MQ=255)
                                                                                                              ttttACTGCATTGATATAAAAAGAAAAAATGATGCCTAAGCATCATTTTTCCTATCTCATTTTGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTATTGAAAGGAAAATTCTCAGCTCCTGCATTTTTGCCCCTAAAgtg                                       <  6:105167/148‑1 (MQ=255)
                                                                                                              ttttACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                      >  3:207807/1‑149 (MQ=255)
                                                                                                              ttttACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                      >  5:339661/1‑149 (MQ=255)
                                                                                                              ttttACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                      <  4:136201/149‑1 (MQ=255)
                                                                                                                 tACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAATGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTa                                    <  4:249392/148‑1 (MQ=255)
                                                                                                                  aCTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAACCCTATTTTCTTGAAAGGAAAATTCTCCGCGCCTGCCTTTTTGCCCCTAAAGTGTTACt                                  >  6:162702/1‑149 (MQ=255)
                                                                                                                      cATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTcc                                <  2:102592/147‑1 (MQ=255)
                                                                                                                       aTTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTg                              >  2:12424/1‑148 (MQ=255)
                                                                                                                               aaaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTCCCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCg                      >  8:265018/1‑148 (MQ=255)
                                                                                                                               aaaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTa                                    >  3:148032/1‑134 (MQ=255)
                                                                                                                               aaaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTa                                    <  4:148032/134‑1 (MQ=255)
                                                                                                                                aaaaGAAAAAATGATGCCAAAGCCTCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGa                     >  5:53341/1‑148 (MQ=255)
                                                                                                                                aaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGa                     >  3:118109/1‑148 (MQ=255)
                                                                                                                                  ataggaaaaaTGATGCTTTGGCATCATTTTTTCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGaaa                   >  5:75893/6‑149 (MQ=255)
                                                                                                                                  aaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTTTCAAACCGCTACCCACGAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTACAGTGTTAAGCCTGAAGCAGCGcaa                   >  4:222958/1‑148 (MQ=255)
                                                                                                                                  aaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAAc                  <  1:328748/149‑1 (MQ=255)
                                                                                                                                  aaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAAc                  <  6:252313/149‑1 (MQ=255)
                                                                                                                                      aaaaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGGATCAAAACGCTACCCACTAATGGAGGGAACCCTAGTTTCTTGACAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCAAAAGTGTTACTCCTCAAGCAGAGAAACaa                >  5:215274/1‑148 (MQ=255)
                                                                                                                                       aaaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAAt              <  5:239740/149‑1 (MQ=255)
                                                                                                                                        aaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATc             <  3:236807/149‑1 (MQ=255)
                                                                                                                                         aaaTGATGCCAACGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACACACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAATTGTTACTCCTGAAGCAGCGAAACaaagc             >  5:274908/1‑146 (MQ=255)
                                                                                                                                         aaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATc             >  3:32579/1‑148 (MQ=255)
                                                                                                                                          aaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCg            >  8:137245/1‑148 (MQ=255)
                                                                                                                                           atgatgCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGaa          >  5:291309/1‑149 (MQ=255)
                                                                                                                                           atgatgCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGaa          <  6:339661/149‑1 (MQ=255)
                                                                                                                                           atgatgCCAAAGCATCATTTTTCCGATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCGTGAAAGGAAATTTCTCAGCGCCTTCATTTTTGCCCCTAAAGTGTTACTCATGAAGCAGCGAAACAAATctac          >  5:260239/1‑146 (MQ=255)
                                                                                                                                                  cAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAgcgc                                                             >  7:97053/1‑91 (MQ=255)
                                                                                                                                                  cAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTc                                 <  6:234835/119‑1 (MQ=255)
                                                                                                                                                  cAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTc                                 >  5:234835/1‑119 (MQ=255)
                                                                                                                                                  cAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCAtttt   >  5:170298/1‑149 (MQ=255)
                                                                                                                                                   aaaGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTc  <  1:159536/149‑1 (MQ=255)
                                                                                                                                                   aaaGCATCATTTTTCCTATCTCAGTTGGTAACAAAACGCTACCCTCTAATGGAGTGAAACCTATTTTCTTGAAAGTAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTc  <  1:280120/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTC  >  NC_000913/3792620‑3792914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: