Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,076,604 G→A 6.7% L153L (CTG→CTA)  yihY → BrkB family putative transporter, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,076,6040GA 6.7% 233.0 / 4.7 74L153L (CTG→CTA) yihYBrkB family putative transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (32/37);  new base A (2/3);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

TTGGGGCGTGCGGGCTGATCGTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTGTTCCTAC  >  NC_000913/4076456‑4076750
                                                                                                                                                    |                                                                                                                                                  
ttGGGGCGTGCGGGCTGATCGTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGctgctg                                                                                                                                                    <  2:214731/149‑1 (MQ=255)
   gggCGTGCGGGCTGATCGTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCa                                                                                                                                                 <  3:211650/149‑1 (MQ=255)
    ggCGTGCGGGCTGATCGTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAg                                                                                                                                                <  3:296276/149‑1 (MQ=255)
    ggCGTGCGGGCTGATCGTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAg                                                                                                                                                >  4:56242/1‑149 (MQ=255)
     gagTGCGGGCTGAGCGTCACCGCGTTATTGTTTATGTACTCCAGCGATATCGCGTTCAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCAGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAgg                                                                                                                                               <  6:21449/147‑1 (MQ=255)
     gCGTGCGGGCTGATCGTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTTCTCGTTCGCCGTGTACTGGATGATTTTAAGGCTGGGGCCGCTGCTGGCAgg                                                                                                                                               <  8:188493/149‑1 (MQ=255)
     gCGTGCGGGCTGATCGTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAgg                                                                                                                                               <  5:90422/149‑1 (MQ=255)
        tGCGGGCTGATCGTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCCCTGCCAGCAGCGGc                                                                                                                                            <  4:201697/149‑1 (MQ=255)
        tGCGGGCTGATCGTCACCGCGGTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGc                                                                                                                                            <  2:219284/149‑1 (MQ=255)
            ggCTGATCGTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCCCTGCCAGCAGCGGcccca                                                                                                                                        <  2:200653/149‑4 (MQ=255)
                 aTCGTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTgg                                                                                                                                    <  3:56242/148‑1 (MQ=255)
                  tCGTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGc                                                                                                                                   >  3:261494/1‑148 (MQ=255)
                    gTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTTACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGa                                                                                                                                 <  6:259874/148‑1 (MQ=255)
                       aCCGCGTTATTGTTGATGTACTCCATCGATAGCGCTTTGAATAACATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCa                                                                                                                              <  2:346506/148‑1 (MQ=255)
                       aCCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAggg                                                                                                                                              >  3:250968/1‑132 (MQ=255)
                       aCCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAggg                                                                                                                                              <  4:250968/132‑1 (MQ=255)
                       aCCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAg                                                                                                                             >  5:116124/1‑149 (MQ=255)
                               aTTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTAt                                                                                                                      <  7:214677/148‑1 (MQ=255)
                                   ttgATGTACTCACTCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGct                                                                                                                 <  4:134679/149‑1 (MQ=255)
                                     gATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGctct                                                                                                               >  7:46225/1‑149 (MQ=255)
                                     gATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGctc                                                                                                                <  7:343602/148‑1 (MQ=255)
                                          aCTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCt                                                                                                           <  2:317458/148‑1 (MQ=255)
                                           cTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTAGTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTgc                                                                                                         <  8:165997/149‑1 (MQ=255)
                                            tCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTAGTTCGCCGTGTACTGGATGATTTTACCGCTGGTGCCGCTTCTGGCAGGGTCCAGTCTGGCGAGCAGGTCCTATTTGCTCTCACTgct                                                                                                        >  4:21827/1‑148 (MQ=255)
                                              cATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCt                                                                                                      >  2:143720/1‑149 (MQ=255)
                                               atagATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGCCCAAAAAATTACTCGTTCGCCGTGTACTCGATGATTTTAACGCTGGGGCCGCTGCTGGAAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTg                                                                                                     >  5:251589/4‑149 (MQ=255)
                                                  gATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGgc                                                                                                  <  1:61432/149‑1 (MQ=255)
                                                  gATAGCGCGTTGAATACAATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGgc                                                                                                  <  4:324358/149‑1 (MQ=255)
                                                       cgcgTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGgcgagcg                                                                                             <  6:41808/149‑1 (MQ=255)
                                                             gAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGAtctc                                                                                        >  6:38913/1‑148 (MQ=255)
                                                              aaTACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTGCGACGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCGCTGCGCTGGGCGAGCGATCTCaa                                                                                      <  5:142672/149‑1 (MQ=255)
                                                               aTACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCaa                                                                                      >  1:205963/1‑148 (MQ=255)
                                                                tACCATCTGGCGCAGTAAACTAGCGCGACCCAAAATTTACTCTTTCGCCGTGTACTTTATGATTTTAACGCTGGGTCCGCTGCTGGAAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATa                                                                                    <  8:155219/149‑1 (MQ=255)
                                                                tACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATa                                                                                    >  1:123660/1‑149 (MQ=255)
                                                                  ccATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGgcgagc                                                                                              >  1:255818/1‑137 (MQ=255)
                                                                  ccATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGgcgagc                                                                                              <  2:255818/137‑1 (MQ=255)
                                                                   cATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTACCGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGAGATCAGTTCCTATTAGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTg                                                                                 >  1:322014/1‑149 (MQ=255)
                                                                         gcgcAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTc                                                                               <  5:38913/145‑1 (MQ=255)
                                                                           gcAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATc                                                                            <  7:190576/146‑1 (MQ=255)
                                                                           gcAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATc                                                                            >  8:190576/1‑146 (MQ=255)
                                                                           gcAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGa                                                                          >  5:193644/1‑148 (MQ=255)
                                                                           gcAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGa                                                                          >  8:227051/1‑148 (MQ=255)
                                                                             aGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACaa                                                                       <  6:193644/149‑1 (MQ=255)
                                                                                  aCGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGtgc                                                                  <  6:233287/149‑1 (MQ=255)
                                                                                    gAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTACCGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGtgctg                                                                >  7:261610/1‑149 (MQ=255)
                                                                                      gcgcGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCg                                                              >  2:82722/1‑149 (MQ=255)
                                                                                          gcccAAAAATTTACTCGTTCGCCGTGTACTGGAGGATGTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTTGTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGGCATCGACCCCGTGCTGCGGAtt                                                          >  2:236948/3‑149 (MQ=255)
                                                                                             cAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTAttttt                                                       <  7:220151/149‑1 (MQ=255)
                                                                                             cAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTAttttt                                                       <  1:166088/149‑1 (MQ=255)
                                                                                                aaaTTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCg                                                    >  7:180803/1‑149 (MQ=255)
                                                                                                     tACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGc                                                <  1:143720/148‑1 (MQ=255)
                                                                                                      aCTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGtgct                                                                 >  4:181667/1‑130 (MQ=255)
                                                                                                      aCTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGtgct                                                                 <  3:181667/130‑1 (MQ=255)
                                                                                                       cTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCtgt                                             >  7:97801/1‑149 (MQ=255)
                                                                                                        tCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCtgtt                                            >  3:45506/1‑149 (MQ=255)
                                                                                                          gTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCtgttgt                                          >  1:84079/1‑149 (MQ=255)
                                                                                                              gCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGt                                       <  3:173917/148‑1 (MQ=255)
                                                                                                              gCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTg                                      <  8:180803/149‑1 (MQ=255)
                                                                                                                   gtACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGAtct                                 >  8:317148/1‑149 (MQ=255)
                                                                                                                    tACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGAtctc                                >  5:226485/1‑149 (MQ=255)
                                                                                                                       tGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCtt                             >  1:163747/1‑149 (MQ=255)
                                                                                                                         gatgatTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCt                           >  7:233730/1‑149 (MQ=255)
                                                                                                                            gatTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGt                        >  3:327093/1‑149 (MQ=255)
                                                                                                                              ttttAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGTCGAGCGATCTCACTACAGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTg                      >  4:263032/1‑149 (MQ=255)
                                                                                                                              ttttAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTc                                         >  1:241449/1‑130 (MQ=255)
                                                                                                                              ttttAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTc                                         <  2:241449/130‑1 (MQ=255)
                                                                                                                              ttttAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGAGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTg                      <  8:261610/149‑1 (MQ=255)
                                                                                                                                ttAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGc                     <  2:84079/148‑1 (MQ=255)
                                                                                                                                   ccagactggCCCCTGCCAGCAGCGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTa                 >  1:235055/8‑149 (MQ=255)
                                                                                                                                         gggCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCa            <  8:46225/148‑1 (MQ=255)
                                                                                                                                          ggCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCAtt          <  2:205963/149‑1 (MQ=255)
                                                                                                                                           ggcGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGGGCGATCTCAATACTGTCATCGACAACGTGGTGCGGATTTATCCGTGGCTGTTCTCGTTGATCTCCATCTGGTTGCAGTAAAGAATTg         >  7:158457/3‑149 (MQ=255)
                                                                                                                                           gCCGCTGCTAGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACa               <  7:289860/143‑1 (MQ=255)
                                                                                                                                           gCCGCTGCTAGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACa               >  8:289860/1‑143 (MQ=255)
                                                                                                                                             cGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTGtt       <  6:116124/149‑1 (MQ=255)
                                                                                                                                               ctgctgGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTTCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTGTTcc     <  2:163747/149‑1 (MQ=255)
                                                                                                                                                 gctgGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGAtct                                                                                         <  8:127521/63‑1 (MQ=255)
                                                                                                                                                 gctgGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGAtct                                                                                         >  7:127521/1‑63 (MQ=255)
                                                                                                                                                 gctgGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTGTTCCTa   <  8:97801/149‑1 (MQ=255)
                                                                                                                                                 gctgGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTGTTCCTa   <  4:261494/149‑1 (MQ=255)
                                                                                                                                                  gtgGCAGGGGCCCGTCTGGCGATGAGTTCCTATTTGCGCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACATCGTGCTTCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTTCTGTACAGCATTGTTCCTAc  <  6:251589/148‑1 (MQ=255)
                                                                                                                                                  ctgGCAGGGTCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTGTTCCTAc  <  6:226485/149‑1 (MQ=255)
                                                                                                                                                  ctgGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTGTTCCTAc  >  6:309768/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTGGGGCGTGCGGGCTGATCGTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTGTTCCTAC  >  NC_000913/4076456‑4076750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑