Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 624,665 G→C 5.6% intergenic (‑155/‑220) fepB ← / → entC ferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913624,6650GC 5.6% 275.3 / 5.4 85intergenic (‑155/‑220)fepB/entCferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1
Reads supporting (aligned to +/- strand):  ref base G (42/38);  new base C (2/3);  total (44/41)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

TGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGC  >  NC_000913/624523‑624808
                                                                                                                                              |                                                                                                                                               
tGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAgg                                                                                                                                             >  7:56358/1‑147 (MQ=255)
  ttattaATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCCCCCCAGCCCTCACCCTGGAAGGga                                                                                                                                           >  1:214236/1‑147 (MQ=255)
  ttattaATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGga                                                                                                                                           <  4:15742/147‑1 (MQ=255)
  ttattaATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGga                                                                                                                                           >  7:308301/1‑147 (MQ=255)
  ttattaATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGga                                                                                                                                           >  3:96385/1‑147 (MQ=255)
  ttattaATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCATCACCCCAGCCCTCACCCTGGAAgaga                                                                                                                                           >  6:288590/1‑147 (MQ=255)
    attaATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGagag                                                                                                                                        <  2:214236/148‑1 (MQ=255)
      taataaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCAAAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCTCCCTTCCAGGGTGAggg                                                                                                                                      <  6:60478/148‑1 (MQ=25)
        ataaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCTCCCTTCCAGGGTGAgggctg                                                                                                                                   <  1:9789/149‑4 (MQ=25)
        ataaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCCTCCCCTCGACCCTTTGTGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAggggg                                                                                                                                    <  7:245032/148‑1 (MQ=255)
         taaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGTGTCAGCGGAACAGCCAGCACTGCTCCGAACATAAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCa                                                                                                                                  >  5:295874/1‑149 (MQ=37)
           aGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAg                                                                                                                                 >  6:287238/1‑148 (MQ=255)
              ttAAGGGCGTAATGACTAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTACCCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAAcc                                                                                                                             >  6:324675/1‑148 (MQ=255)
                    gCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGTTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAg                                                                                                                       <  4:27853/149‑1 (MQ=255)
                    gCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAg                                                                                                                       <  7:181588/149‑1 (MQ=255)
                          tGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATc                                                                                                                 <  5:297053/149‑1 (MQ=255)
                            aCAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATca                                                                                                                <  3:108493/148‑1 (MQ=255)
                            aCAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATca                                                                                                                >  3:196087/1‑148 (MQ=255)
                              aaaTTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACAt                                                                                                             >  7:150731/1‑149 (MQ=255)
                                aTTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTg                                                                                                           >  6:202972/1‑149 (MQ=255)
                                  tCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTgcg                                                                                                         >  8:40021/1‑149 (MQ=255)
                                     aCAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCt                                                                                                       >  7:115269/1‑148 (MQ=255)
                                       aatgcgcACAATCCGTCCCCTCGCCCGTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAAGCCTCACCCCAGCCCTGACGCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTAt                                                                                                    <  6:295874/146‑1 (MQ=37)
                                       aaaGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTAt                                                                                                    <  4:96385/149‑1 (MQ=255)
                                         aGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGc                                                                                                  <  6:307733/149‑1 (MQ=255)
                                         aGCGCACAATCCGTCCCCTCGCCCATTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGc                                                                                                  <  6:303859/149‑1 (MQ=255)
                                             cacaATCCGTCCGCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCTCTGGTGCGAACATTAAACCTCACCGCAGCCCTGACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAAt                                                                                              <  2:308118/149‑1 (MQ=37)
                                             cacaATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAAt                                                                                              <  4:196087/149‑1 (MQ=255)
                                                  tCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGga                                                                                                                                           >  2:260397/1‑99 (MQ=255)
                                                  tCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGga                                                                                                                                           <  1:260397/99‑1 (MQ=255)
                                                     gTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGATCATTAACCCTCCCCCCAGCCCTCACCCTGCAAGGGAGAGGGGGCAGAACGGCGCAGGACAGCACATTGCGCTTATGCGAATCCATCaa                                                                                       >  3:302031/1‑148 (MQ=255)
                                                        accTCGCCCCGTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGTAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCaataat                                                                                   <  6:305599/148‑1 (MQ=255)
                                                            cGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCAGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTCCGCTAATGCGAAGCCATCAATAATGCt                                                                                >  4:205168/1‑148 (MQ=255)
                                                             gCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTtc                                                                              >  5:290437/1‑149 (MQ=255)
                                                                ccTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCa                                                                           <  8:56358/149‑1 (MQ=255)
                                                                ccTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGCGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTtctcc                                                                           >  3:272282/1‑148 (MQ=255)
                                                                   ttGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCAttt                                                                        >  5:243307/1‑149 (MQ=255)
                                                                     ggggAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATg                                                                                  <  2:166418/137‑1 (MQ=255)
                                                                     ggggAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATg                                                                                  >  1:166418/1‑137 (MQ=255)
                                                                      gggAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCa                                                                     <  7:219397/149‑1 (MQ=255)
                                                                      gggAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCa                                                                     >  8:219397/1‑149 (MQ=255)
                                                                      gggAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCAGCCCTCACCCTGGAAGGGAGAGGGGGGAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCa                                                                     <  6:198319/148‑1 (MQ=255)
                                                                       ggAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCAt                                                                    <  6:290437/149‑1 (MQ=255)
                                                                        gagaGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCAtt                                                                   <  5:287238/149‑1 (MQ=255)
                                                                          gagGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCaataat                                                                                   <  1:115900/131‑1 (MQ=255)
                                                                          gagGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCaataat                                                                                   >  2:115900/1‑131 (MQ=255)
                                                                              gTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAAc                                                              >  7:25529/1‑148 (MQ=255)
                                                                               ttAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACca                                                            <  5:202972/149‑1 (MQ=255)
                                                                               ttAGGGTCAGGGGAACAGCCAGCACTGGTGCGAACATTAATCCTCAACCCAGCCCTCAACCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCGTCTCATTTTCATTGTAACca                                                            >  4:227038/1‑149 (MQ=255)
                                                                                   ggTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATca                                                                                                                >  7:267995/1‑93 (MQ=255)
                                                                                   ggTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATca                                                                                                                <  8:267995/93‑1 (MQ=255)
                                                                                   ggTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAAc                                                        <  7:40021/149‑1 (MQ=255)
                                                                                       aGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAg                                                     <  6:243307/148‑1 (MQ=255)
                                                                                         gggAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATg                                                  >  6:138595/1‑149 (MQ=255)
                                                                                            aaCAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCaa                                               >  1:53714/1‑149 (MQ=255)
                                                                                               agccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAAccc                                            >  5:277357/1‑149 (MQ=255)
                                                                                                 ccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACcac                                                           <  2:127611/132‑1 (MQ=255)
                                                                                                 ccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACcac                                                           >  1:127611/1‑132 (MQ=255)
                                                                                                       cTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACCTTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGc                                    <  4:22826/149‑1 (MQ=255)
                                                                                                          gTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCa                                   <  8:150731/147‑1 (MQ=255)
                                                                                                            gCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGAtt                               >  3:154073/1‑149 (MQ=255)
                                                                                                             cGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGCGGGCAGAACGGCGCAGGACATCACATTGCTCTTATGCGAATCCATCAATAATGCTTCTCATATTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTg                              >  2:113413/1‑149 (MQ=255)
                                                                                                              gAACATTAACCCTCACCCCAGCCCTCTCCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCCCTTTGCGCTTATGCGAATCCATCAATCATGCTTCTCATTTTCATTGTACCCACAACCAGATGCAACCCCGAGTTGCAGATTGc                             <  6:83754/149‑1 (MQ=255)
                                                                                                                aCATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGt                           <  8:308301/149‑1 (MQ=255)
                                                                                                                 cATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGt                           >  3:125470/1‑148 (MQ=255)
                                                                                                                  aTTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTa                         >  2:64433/1‑149 (MQ=255)
                                                                                                                  aTTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTa                         >  6:162826/1‑149 (MQ=255)
                                                                                                                    tAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAAc                                                              <  2:37444/110‑1 (MQ=255)
                                                                                                                    tAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAAc                                                              >  1:37444/1‑110 (MQ=255)
                                                                                                                     aaCCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTAcc                       <  6:277357/148‑1 (MQ=255)
                                                                                                                      aCCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCt                      >  4:321546/1‑148 (MQ=255)
                                                                                                                        ccTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTc                     >  4:268750/1‑147 (MQ=255)
                                                                                                                         cTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTc                     >  5:101119/1‑146 (MQ=255)
                                                                                                                             ccacAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGtt              <  1:233279/146‑1 (MQ=255)
                                                                                                                              cccAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTg             <  4:151230/149‑1 (MQ=255)
                                                                                                                                   cccTCTCCCTTCCAGGGTGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGtt              >  8:70444/1‑143 (MQ=255)
                                                                                                                                   cccTCTCCCTTCCAGGGTGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGtt              <  7:70444/143‑1 (MQ=255)
                                                                                                                                    ccTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAg       >  7:230521/1‑149 (MQ=255)
                                                                                                                                    ccTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAg       >  1:120971/1‑149 (MQ=255)
                                                                                                                                     cTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGt      <  3:321546/149‑1 (MQ=255)
                                                                                                                                     cTCACACTGGAAGGGAGAGGGGGCAGAACGTCGCAGTACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGt      <  5:288590/149‑1 (MQ=255)
                                                                                                                                      tCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCAtt                                                                   >  2:109159/1‑87 (MQ=255)
                                                                                                                                      tCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCAtt                                                                   <  1:109159/87‑1 (MQ=255)
                                                                                                                                      tCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTg     >  3:322403/1‑149 (MQ=255)
                                                                                                                                         cccTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTgcgc  >  5:249678/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                               
TGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGC  >  NC_000913/624523‑624808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: