Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,447,508 T→A 5.2% F1133I (TTC→ATC)  tamB → translocation and assembly module for autotransporter export, inner membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,447,5080TA 5.2% 250.3 / 3.3 77F1133I (TTC→ATC) tamBtranslocation and assembly module for autotransporter export, inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (34/39);  new base A (2/2);  total (36/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

TGCGTAAAGGTGAGTTACTGTTCTCTGGTCCGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTGGGGGTTGCGCAAAGTGGCCAGATTGTG  >  NC_000913/4447362‑4447648
                                                                                                                                                  |                                                                                                                                            
tgCGTAAAGGTGAGTTACTGTTCTCTGGTCCGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGAtcttc                                                                                                                                            <  4:57747/149‑1 (MQ=255)
tgCGTAAAGGTGAGTTACTGTTCTCTGGTCCGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGAtcttc                                                                                                                                            <  2:102654/149‑1 (MQ=255)
   gTAAAGGTGAGTTACTGTTCTCTGGTCCGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTctct                                                                                                                                         >  3:125935/1‑149 (MQ=255)
         gTGAGTTACTGTTCTCTGGTCCGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCg                                                                                                                                   >  3:32732/1‑149 (MQ=255)
              ttACTGTTCTCTGGTCCGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGAt                                                                                                                              >  1:288844/1‑149 (MQ=255)
                 cTGTTCTCTGCTCCGCCAGAACAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTc                                                                                                                           <  2:346626/149‑1 (MQ=255)
                    ttCTCTGGTCCGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCg                                                                                                                          <  8:302143/147‑1 (MQ=255)
                              cGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGcc                                                                                                              <  2:140798/149‑1 (MQ=255)
                               gCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCAGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCt                                                                                                             >  4:198333/1‑149 (MQ=255)
                                ccAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGTCGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGa                                                                                                                               <  3:201272/130‑1 (MQ=255)
                                ccAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGTCGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGa                                                                                                                               >  4:201272/1‑130 (MQ=255)
                                ccAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCtt                                                                                                            <  4:32732/149‑1 (MQ=255)
                                       aaCCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTAt                                                                                                     <  6:205335/149‑1 (MQ=255)
                                       aaCCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTAt                                                                                                     >  1:175566/1‑149 (MQ=255)
                                        aCCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTAtt                                                                                                    <  2:175566/149‑1 (MQ=255)
                                        aCCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTAtt                                                                                                    >  4:197326/1‑149 (MQ=255)
                                              tCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTctct                                                                                                                                         <  3:111430/106‑1 (MQ=255)
                                              tCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTctct                                                                                                                                         >  4:111430/1‑106 (MQ=255)
                                               cTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCATCTCTGACCCGGCGAt                                                                                                                              >  3:142232/1‑116 (MQ=255)
                                               cTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCATCTCTGACCCGGCGAt                                                                                                                              >  3:147252/1‑116 (MQ=255)
                                               cTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCATCTCTGACCCGGCGAt                                                                                                                              <  4:147252/116‑1 (MQ=255)
                                               cTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCATCTCTGACCCGGCGAt                                                                                                                              <  4:142232/116‑1 (MQ=255)
                                                    tatTGAAGCTATTCGTAACCCGGATGCTACAGAAGCCGCCGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGa                                                                                         >  5:10104/1‑148 (MQ=255)
                                                    tatTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGa                                                                                         >  8:254746/1‑148 (MQ=255)
                                                        gAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAgg                                                                                    >  7:301323/1‑149 (MQ=255)
                                                             tATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTg                                                                                >  3:227876/1‑148 (MQ=255)
                                                              aTTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGc                                                                                                 >  4:102609/1‑130 (MQ=255)
                                                              aTTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGc                                                                                                 <  3:102609/130‑1 (MQ=255)
                                                                  gTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAgg                                                                                    <  8:24661/139‑1 (MQ=255)
                                                                  gTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAgg                                                                                    >  7:24661/1‑139 (MQ=255)
                                                                  gTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGc                                                                          >  1:46734/1‑149 (MQ=255)
                                                                       ccGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCa                                                                     <  2:147083/149‑1 (MQ=255)
                                                                         ggATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCaga                                                                   <  4:125935/149‑1 (MQ=255)
                                                                           aTGCTACAGAATACGACGTAATCGCCGGAGTTCGCGTCACTGTTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGAGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTTGACAAGGGCTGGAGAGCGATCAGAGc                                                                 <  6:23258/149‑1 (MQ=255)
                                                                                aCAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAg                                                            >  5:173376/1‑149 (MQ=255)
                                                                                  agaagaCTACGTAATCGCCGGAGTTAGCGTCACTGGTCTGGCGGACGAACCGAAAGAGGAGATCTTCTCTGACCCGGCGATTTCGCAACAAGCTGCATTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTg                                                          >  1:294033/1‑149 (MQ=255)
                                                                                     agaagaCGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGAAACAAGCTGCCTGGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCgg                                                       <  6:165003/149‑1 (MQ=255)
                                                                                         gacgTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGa                                                                        <  3:6670/128‑1 (MQ=255)
                                                                                         gacgTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGa                                                                        >  4:6670/1‑128 (MQ=255)
                                                                                         gacgTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAAt                                                   >  7:180355/1‑149 (MQ=255)
                                                                                         gacgTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAAt                                                   <  3:198333/149‑1 (MQ=255)
                                                                                         gacgTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAAt                                                   >  7:142950/1‑149 (MQ=255)
                                                                                         gacgTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGAGCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAAt                                                   <  5:189707/149‑1 (MQ=255)
                                                                                             tattagCCGGAGTTCGCGTCTCTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCATTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGAc                                                <  8:159758/143‑1 (MQ=255)
                                                                                             tAATCGCCGGAGTTCGCGTCACTGTTTTGTGTTAATTACAGATAGAGGAGATATTTTATGACACGGCTATGTCGCAACTAGCTGCCTTTTCTTATTTGGTTCTTTTACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGAcc                                               <  2:218630/149‑1 (MQ=255)
                                                                                               aTCGCCGGAGTTCGCGTGACTGGTCTGGCGGTCGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCt                                              <  6:253724/148‑1 (MQ=255)
                                                                                                  gCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTCCGTTGACAAGGGCTGGAGAGCGATCAGAGCTACAGTGCGGCAATGACCTAGAt                                          >  2:218236/1‑149 (MQ=255)
                                                                                                  gCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGAt                                          >  3:152422/1‑149 (MQ=255)
                                                                                                  gCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGAGCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGAt                                          <  3:211520/149‑1 (MQ=255)
                                                                                                         ttCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGAtt                                   >  7:172134/1‑149 (MQ=255)
                                                                                                         ttCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGAtt                                   <  6:10104/149‑1 (MQ=255)
                                                                                                         ttCGCGGCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGAtt                                   <  6:196008/149‑1 (MQ=255)
                                                                                                            gcgTCACTGGTCTGGCGGACGAACCGAAAGCGTAGATCTTCTCTGCCCCGGCGATGTCTCAACAAGCTGCCTTGTCTAATTTCCTACGTGAACAAGGGCTGGAGAGCCATCATAGCGACAGTGCGGAAATGAACTCGATGATGATAGGt                                >  6:270641/1‑149 (MQ=255)
                                                                                                             cgTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGtt                               <  8:301323/149‑1 (MQ=255)
                                                                                                             cgTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGtt                               <  8:172134/149‑1 (MQ=255)
                                                                                                               tCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGt                                <  4:185872/146‑1 (MQ=255)
                                                                                                               tCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGt                                >  3:185872/1‑146 (MQ=255)
                                                                                                                cACTGGTCTGGCGGACGAACCGAAAGGGGGGATCTTCTCTGAACCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCGGGAGAGCGATCAGAGCGCCGGTGCGGCTATGAGCTCGATGCTGAGTGGTTTgt                            >  4:194520/1‑148 (MQ=255)
                                                                                                                cACTGGTCGGGCGGACGAACCGAATGCGGAGATGTTCTCTGACCGTGCGATGTCGCAAGAAGGTGCCTTGTATTATTTGCTACGTGGACTAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTgg                            <  8:189586/149‑1 (MQ=255)
                                                                                                                 aCTGGTCTGGCTGACGAACCGAAAGCGGAGAGCTTCTCTGACCCGGCGATGTCGCACCAAGCTGCCTTGTCTTATTTGCTCCGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTggg                           <  2:294033/149‑1 (MQ=255)
                                                                                                                 aCTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGAAAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTgg                            <  5:270641/148‑1 (MQ=255)
                                                                                                                     gTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGttt                              >  7:212207/1‑142 (MQ=255)
                                                                                                                     gTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGttt                              <  8:212207/142‑1 (MQ=255)
                                                                                                                     gTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTggggg                         <  4:227876/147‑1 (MQ=255)
                                                                                                                     gTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTGGGGGtt                       <  2:288844/149‑1 (MQ=255)
                                                                                                                     gTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGAGCAGAGCGACAGTGCGGCAATGACCTCGATGCTGAATGGTTTggggg                         <  3:197326/147‑1 (MQ=255)
                                                                                                                       cTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTGGGGGTTgc                     <  6:173376/149‑1 (MQ=255)
                                                                                                                        tGGCGGACGAACCGAAAGCGGAGTTTTTGTCTGCCCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTGGGGGTTgcg                    <  7:128181/149‑1 (MQ=255)
                                                                                                                          gCGGACGCACCGAAAGCGGAGCTCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGCCCTCGATGCTGATTGGTTAGGGCGTTgcgc                   >  4:146774/1‑148 (MQ=255)
                                                                                                                             gACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTGGGGGTTGCGCAAAg               <  8:180355/149‑1 (MQ=255)
                                                                                                                                gAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTGGGGGTTGCGCAAAGTgg            >  6:12983/1‑149 (MQ=255)
                                                                                                                                  aCCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTCGGTTGGGGGTTCCGCAAAGTGGc           >  2:278816/1‑148 (MQ=255)
                                                                                                                                   ccGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCt                                              <  4:245968/112‑1 (MQ=255)
                                                                                                                                    cGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTGGGGGTTGCGCAAAGTGGc           >  7:159407/1‑146 (MQ=255)
                                                                                                                                     gAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTGGGGGTTGCGCAAAGTGGc           >  5:260166/1‑145 (MQ=255)
                                                                                                                                        aGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTGGGGGTTGCGCAAAGTGGCCAGATtg    >  2:227979/1‑149 (MQ=255)
                                                                                                                                         gCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTGGGGGTTGCGCAAAGTGGCCAGATtgt   <  8:142950/149‑1 (MQ=255)
                                                                                                                                         gCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGGGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTGGGGGTTGCGCAAAGTGGCCAGATtgt   <  1:218236/149‑1 (MQ=255)
                                                                                                                                          cGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTGGGGGGTTGCGCAAAGTGGCCAGATtgtg  >  8:244782/1‑149 (MQ=255)
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TGCGTAAAGGTGAGTTACTGTTCTCTGGTCCGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGAGTTCGCGTCACTGGTCTGGCGGACGAACCGAAAGCGGAGATCTTCTCTGACCCGGCGATGTCGCAACAAGCTGCCTTGTCTTATTTGCTACGTGGACAAGGGCTGGAGAGCGATCAGAGCGACAGTGCGGCAATGACCTCGATGCTGATTGGTTTGGGGGTTGCGCAAAGTGGCCAGATTGTG  >  NC_000913/4447362‑4447648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: