Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 755,096 C→T 5.3% intergenic (‑628/‑81) gltA ← / → sdhC citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913755,0960CT 5.3% 231.4 / 2.3 76intergenic (‑628/‑81)gltA/sdhCcitrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556
Reads supporting (aligned to +/- strand):  ref base C (33/37);  new base T (2/2);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

TGCCGCCAGGTCTCCGGAACACCCTGCAATCCCGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCAC  >  NC_000913/754948‑755244
                                                                                                                                                    |                                                                                                                                                    
tGCCGCCAGGTCTCCGGAACACCCTGCAATCCCGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATccc                                                                                                                                                      >  6:119952/1‑149 (MQ=255)
  ccgccAGGTCTCCGGAACACCCTGCAATCCCGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGa                                                                                                                                                    <  2:94098/149‑1 (MQ=255)
     ccAGGTCTCCGGAACACCCTGCAATCCCGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGt                                                                                                                                                 <  2:5650/149‑1 (MQ=255)
      cAGGTCTCCGGAACACCCTGCAATCCCGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGAc                                                                                                                                                   <  8:219277/146‑1 (MQ=255)
      cAGGTCTCCGGAACACCCTGCAATCCCGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGAc                                                                                                                                                   >  7:219277/1‑146 (MQ=255)
          tctcCGGAACACCCTGCAATCCCGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGtctc                                                                                                                                              >  2:67545/1‑147 (MQ=255)
          tctcCGGAACACCCTGCAATCCCGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTAAAGAAAGGACGCTGTCTGCCCCGCAAGCAGACCGGAGGAAGGAAATCCAGACGtctc                                                                                                                                              >  6:1422/1‑147 (MQ=255)
              cGGAACACCCTGCAATCCCGAGCCCCCCAGCGTTGAAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCCGTAATAGAAAGGCCGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGACACCAgga                                                                                                                                         >  2:88856/1‑147 (MQ=255)
              cGGAACACCCTGCAATCCCGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGt                                                                                                                                         >  1:180693/1‑148 (MQ=255)
              cGGAACACCCTGCAATCCCGAGCCACCCAGCGTTGTAAAGTGTCGTTTTCGAATATGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGATGTCTGACACGCAAGTAGACCGGAGTAATGAAATCCCGACGTCTCCAGGt                                                                                                                                         >  4:18154/1‑148 (MQ=255)
                gAACACCCTGCAATCCCGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAAc                                                                                                                                      <  1:67545/149‑1 (MQ=255)
                     cccTGCAATCCCGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGaaa                                                                                                                                 <  5:118433/149‑1 (MQ=255)
                       cTGCAATCCCGAGCCACCAAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGt                                                                                                                               >  3:175904/1‑149 (MQ=255)
                             tCCCGAGCCCCCCAGCGTTGTAACGTGTCGTTGTCGCATCTGGAAGCCGTGTTTTGCATGACGCGCAGTTATAGACAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACct                                                                                                                         >  2:319977/1‑149 (MQ=255)
                              cccGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCTCAAGCAGACCGGAGGAACGAAATCCCGACGACTCCAGGTAACAGAAAGTTAACctc                                                                                                                        >  2:87585/1‑149 (MQ=255)
                               ccGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAACGTTAACctct                                                                                                                       <  1:233723/149‑1 (MQ=255)
                               ccGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACctct                                                                                                                       <  5:49606/149‑1 (MQ=255)
                                 gAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCtgt                                                                                                                     >  5:116061/1‑149 (MQ=255)
                                      aCCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCg                                                                                                                >  8:23676/1‑149 (MQ=255)
                                        ccAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTa                                                                                                              <  8:33772/149‑1 (MQ=255)
                                          aGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGt                                                                                                            >  5:325378/1‑149 (MQ=255)
                                              ttGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGt                                                                                                                               >  8:226494/1‑126 (MQ=255)
                                              ttGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGt                                                                                                                               <  7:226494/126‑1 (MQ=255)
                                                gTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACTCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAg                                                                                                      >  3:328881/1‑149 (MQ=255)
                                                 tAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGTAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCACGTAGTCCCCAgg                                                                                                     <  3:18154/149‑1 (MQ=255)
                                                 tAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAg                                                                                                      <  7:5252/148‑1 (MQ=255)
                                                 tAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAg                                                                                                      <  4:283669/148‑1 (MQ=255)
                                                   aCGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAggg                                                                                                    <  7:296882/148‑1 (MQ=255)
                                                   aCGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAggg                                                                                                    <  2:180693/148‑1 (MQ=255)
                                                     gtgtCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACTCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGaa                                                                                                  >  1:83744/1‑148 (MQ=255)
                                                     gtgtCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGaa                                                                                                  >  1:79188/1‑148 (MQ=255)
                                                      tgtCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGaata                                                                                                <  7:23676/149‑1 (MQ=255)
                                                          gTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAAtaataa                                                                                            <  1:88856/149‑1 (MQ=255)
                                                           ttttCGCATCTGGAAGCAGTGTTTTGCATGACGCCCAGTTATAGAAAGGAAGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCACAACCTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGGAGTCCCAAGCGAAtaataa                                                                                            >  1:213216/1‑148 (MQ=255)
                                                              tCGCATCTGGACGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGaa                                                                                         <  1:66874/148‑1 (MQ=255)
                                                               cGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAAc                                                                                        <  5:119952/148‑1 (MQ=255)
                                                                gCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACctct                                                                                                                       >  1:289588/1‑116 (MQ=255)
                                                                gCAGCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACctct                                                                                                                       <  2:289588/116‑1 (MQ=255)
                                                                      ggAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTccca                                                                                                        <  5:326861/125‑2 (MQ=255)
                                                                      ggAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTccca                                                                                                        >  6:326861/1‑124 (MQ=255)
                                                                       gAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACTCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCAtgt                                                                                 <  4:328881/147‑1 (MQ=255)
                                                                         aGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAATGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGACCAGCATGTGGGc                                                                             <  4:92705/149‑1 (MQ=255)
                                                                                 tttGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCa                                                                     <  4:324997/149‑1 (MQ=255)
                                                                                    gCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCAGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCAAGTGGGCGTTATTCATGa                                                                  >  3:192974/1‑149 (MQ=255)
                                                                                     cATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGa                                                                  >  3:29646/1‑148 (MQ=255)
                                                                                        gACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCTGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGaata                                                                                                <  3:195569/115‑1 (MQ=255)
                                                                                        gACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCTGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGaata                                                                                                >  4:199897/1‑115 (MQ=255)
                                                                                        gACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCTGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGaata                                                                                                >  4:195569/1‑115 (MQ=255)
                                                                                        gACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCTGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGaata                                                                                                <  3:199897/115‑1 (MQ=255)
                                                                                        gACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGAt                                                                 >  7:84170/1‑146 (MQ=255)
                                                                                            cgcAGTTATAGAAAGCACGCTGTCTGACACGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGCGAATAATAAGAACAGCATGAGGGCGTTATTGATGATAAGAAAt                                                          >  6:159988/1‑149 (MQ=255)
                                                                                             gcAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAAtg                                                         >  5:218873/1‑149 (MQ=255)
                                                                                              cAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAAtgt                                                        <  6:116061/149‑1 (MQ=255)
                                                                                                      gAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGAAGTCTCCAGGTAACAGAAAGTTAACATCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATCTGaaaaaa                                                 >  7:171202/1‑148 (MQ=255)
                                                                                                         aGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACa                                               >  1:75795/1‑147 (MQ=255)
                                                                                                         aGGACGCTGTCTGACCCGCAAGCAGACAGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACa                                               >  5:172300/1‑147 (MQ=255)
                                                                                                            aCGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTACCCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTAGTCATGATTAGAAATGTGAAAAAACAAAGAc                                          >  5:141651/1‑149 (MQ=255)
                                                                                                            aCGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGAc                                          >  4:2554/1‑149 (MQ=255)
                                                                                                              gCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATCCAAGATAAGAAATGTGAAAAAACAAAGACCt                                        <  1:319977/149‑1 (MQ=255)
                                                                                                                tGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAAACTCTGTGCCCGTAGTCCCCAGGCAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGt                                      >  4:214579/1‑149 (MQ=255)
                                                                                                                  tgtGACCCGCAAGCAGACCGGAGGAAGTAAATCCCGACGTCTCCATGTAACAGATAGTTAACCTCTGTTCCCGTAGTCCCCAGTTAATAATAAGAACAGCATGTGGGCGTTTTTCATGATAAGAAATGTGAAAAAACAAAGACCTGt                                      <  1:87585/145‑1 (MQ=255)
                                                                                                                      aCCCGCTAGCAGACCGGAGGAAGGAAATCCCGACGTCTCTAGGTAACAGAAAGTTTTCCTCTGTGCTCGTAGTCCCCAGGGAATAATAAGTACAGCTTGTGGGCGTTATTCATGATAAGAAATGTGAATATACAAAGACCTGTTAAt                                  <  4:175904/147‑1 (MQ=255)
                                                                                                                      aCCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCt                                >  6:57723/1‑149 (MQ=255)
                                                                                                                        ccGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTgg                              >  2:327807/1‑149 (MQ=255)
                                                                                                                        ccGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTgg                              >  7:100701/1‑149 (MQ=255)
                                                                                                                          gCAAGCTGACCGGTGGAAGGATATCCCGACGTCTCCAGTTATCAGAAAGTTAACCTCTGTGCCCGTAGTCGCCAGGGAATAATAATAACAGCATGTGGTCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGAc                            <  8:171202/149‑1 (MQ=255)
                                                                                                                          gCAAGCAGACCGGACGAAGGAAATCCCGACGTCACCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGAc                            <  8:136899/149‑1 (MQ=255)
                                                                                                                              gCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTa                         >  1:116961/1‑148 (MQ=255)
                                                                                                                              gCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTAc                        <  2:75795/149‑1 (MQ=255)
                                                                                                                                 gACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGa                     <  4:29646/149‑1 (MQ=255)
                                                                                                                                      gaggaAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTACCCAGGGAATAATAAGAACAGCAGGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTg                               <  5:153149/134‑1 (MQ=255)
                                                                                                                                       aggaaggaAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATcc               <  2:83744/149‑1 (MQ=255)
                                                                                                                                       aggaaggaAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATcc               <  2:79188/149‑1 (MQ=255)
                                                                                                                                             gaaATCCCGACGTCTCCATGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGTAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTcc         <  5:1422/149‑1 (MQ=255)
                                                                                                                                             gaaATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTcc         <  8:84170/149‑1 (MQ=255)
                                                                                                                                             gaaATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTcc         >  1:149701/1‑149 (MQ=255)
                                                                                                                                               aaTCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTcccc       <  5:57723/149‑1 (MQ=255)
                                                                                                                                                  cccGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCAt     >  3:136863/1‑148 (MQ=255)
                                                                                                                                                    cGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCAc  >  7:102917/1‑149 (MQ=255)
                                                                                                                                                    cGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCAc  <  2:116961/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGCCGCCAGGTCTCCGGAACACCCTGCAATCCCGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATGACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCAGGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCAC  >  NC_000913/754948‑755244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: